Status of single-cell RNA sequencing for reproductive toxicology in zebrafish and the transcriptomic trade-off

IF 4.6
Mackenzie L. Connell, Danielle N. Meyer, Alex Haimbaugh, Tracie R. Baker
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引用次数: 0

Abstract

The utilization of transcriptomic studies identifying profiles of gene expression, especially in toxicogenomics, has catapulted next-generation sequencing to the forefront of reproductive toxicology. An innovative yet underutilized RNA sequencing technique emerging into this field is single-cell RNA sequencing (scRNA-seq), which provides sequencing at the individual cellular level of gonad tissue. ScRNA-seq provides a novel and unique perspective for identifying distinct cellular profiles, including identification of rare cell subtypes. The specificity of scRNA-seq is a powerful tool for reproductive toxicity research, especially for translational animal models including zebrafish. Studies to date not only have focused on ‘tissue atlassing’ or characterizing what cell types make up different tissues but have also begun to include toxicant exposure as a factor that this review aims to explore. Future scRNA-seq studies will contribute to understanding exposure-induced outcomes; however, the trade-offs with traditional methods need to be considered.

用于斑马鱼生殖毒理学的单细胞 RNA-seq 现状和转录组权衡
利用转录组研究确定基因表达谱,特别是在毒物基因组学(TGx)中的应用,使新一代测序(NGS)成为生殖毒理学的前沿技术。单细胞 RNA 测序(scRNA-seq)是这一领域出现的一种创新但未得到充分利用的 RNA 测序技术,可在性腺组织的单个细胞水平上进行测序。ScRNA-seq 提供了一个新颖独特的视角,可用于识别不同的细胞特征,包括识别罕见的细胞亚型。scRNA-seq 的特异性是生殖毒性研究的有力工具,特别是对包括斑马鱼在内的转化动物模型而言。迄今为止的研究主要集中在 "组织分类 "或描述不同组织的细胞类型,但也开始将毒物暴露作为一个因素,本综述旨在探讨这一点。未来的 scRNA-seq 研究将有助于了解暴露诱导的结果,但需要考虑与传统方法的权衡。
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来源期刊
Current opinion in toxicology
Current opinion in toxicology Toxicology, Biochemistry
CiteScore
8.50
自引率
0.00%
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0
审稿时长
64 days
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