Calcified Tissue Research最新文献_第4页

Potentiation of the effects of Solanum malacoxylon extracts on rat intestinal phosphate and calcium absorption by incubation with ruminal fluid. 用瘤胃液培养增强马来氧隆提取物对大鼠肠道磷酸盐和钙吸收的影响。

Calcified Tissue Research Pub Date : 1978-12-08 DOI: 10.1007/BF02013261

A R de Boland, M I Skliar, S Gallego, M Esparza, R L Boland

引用次数: 8

Stimulation of duodenal and ileal absorption of phosphate in the chick by low-calcium and low-phosphorus diets. 低钙低磷饲粮刺激雏鸡十二指肠和回肠对磷酸盐的吸收

Calcified Tissue Research Pub Date : 1978-12-08 DOI: 10.1007/BF02013265

J Fox, A D Care

引用次数: 6

Lead-loaded ion-exchange resin bead as a calcergen. 铅离子交换树脂珠作为钙原。

Calcified Tissue Research Pub Date : 1978-12-08 DOI: 10.1007/BF02013267

G Ellender, D K Sewell, K N Ham, E Storey

引用次数: 1

Some observations on the calcium ion binding to the eggshell matrix. 钙离子与蛋壳基质结合的一些观察。

Calcified Tissue Research Pub Date : 1978-12-08 DOI: 10.1007/BF02013266

G Abatangelo, D Daga-Gordini, I Castellani, R Cortivo

引用次数: 7

Localization of prostaglandin synthetase in chicken epiphyseal cartilage. 前列腺素合成酶在鸡骨骺软骨中的定位。

Calcified Tissue Research Pub Date : 1978-12-08 DOI: 10.1007/BF02013263

F K Northington, T D Oglesby, Y Ishikawa, R E Wuthier

{"title":"Localization of prostaglandin synthetase in chicken epiphyseal cartilage.","authors":"F K Northington, T D Oglesby, Y Ishikawa, R E Wuthier","doi":"10.1007/BF02013263","DOIUrl":"https://doi.org/10.1007/BF02013263","url":null,"abstract":"Prostaglandin synthetase activity in high-speed particulate fractions of chick epiphyseal cartilage has been characterized with respect to cofactor requirements, pH optimum, buffer-ion effects, types of prostaglandins formed, and the distribution of prostaglandin synthetase activity in zones of the epiphyseal plate. Direct homogenization of cartilage was found to be more efficacious than releasing chondrocytes by enzymatic digestion for preparation of prostaglandin synthetase, a homogenization time of 4 min yielding maximal activity. The optimal incubation medium contained 50 mM Tris buffer (pH 7.5), 2.5 mM epinephrine, 1 micronM hemoglobin, 3.25 mM glutathione, 200 microgram/ml enzyme protein, and 5 micronM substrate. Glutathione was effective only if present during homogenization. Rates of PGE2 biosynthesis were linear up to 15 min and then rapidly declined, indicative of self-deactivation. The low levels of PGF2alpha formed, and their decrease after 20 min incubation, suggests the possible presence of degradative enzymes. Prostaglandin synthetase was inhibited by aspirin, indomethacin, and vitamin E, but not vitamin K1. Cation concentrations in the physiological range had only modest effects on prostaglandin biosynthesis, and then only if present during tissue homogenization. In the presence of phosphate buffer, Ca2+ was somewhat inhibitory. Since in the absence of phosphate Ca2+ had no deleterious effect, it is probably that the inhibitory effect was caused by precipitation of calcium phosphate. Hypertrophic and calcified cartilage exhibited significantly higher prostaglandin synthetase activity than the proliferating and maturing zones. The increased synthesis of prostaglandins in the low layers of the growth plate may indicate a role of these factors in chondrocyte differentiation and/or calcification.","PeriodicalId":9389,"journal":{"name":"Calcified Tissue Research","volume":"26 3","pages":"227-36"},"PeriodicalIF":0.0,"publicationDate":"1978-12-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/BF02013263","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"11327565","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Electrical osteogenesis--pro and con. 电成骨——正反两方面。

Calcified Tissue Research Pub Date : 1978-12-08 DOI: 10.1007/BF02013241

R O Becker

引用次数: 8

Effect of solubilized bone matrix components on cultured fibroblasts derived from neonatal rat tissues. 溶解骨基质成分对新生大鼠组织培养成纤维细胞的影响。

Calcified Tissue Research Pub Date : 1978-12-08 DOI: 10.1007/BF02013253

T Anastassiades, O Puzic, R Puzic

引用次数: 9

Intestinal calcium absorption, serum phosphate, and parathyroid hormone in patients with chronic renal failure and osteodystrophy before and during hemodialysis. 慢性肾功能衰竭和骨营养不良患者在血液透析前和透析期间的肠钙吸收、血清磷酸盐和甲状旁腺激素。

Calcified Tissue Research Pub Date : 1978-12-08 DOI: 10.1007/BF02013246

J R Juttmann, J C Hagenouw-Taal, L D Lameyer, A M Ruis, J C Birkenhäger

引用次数: 4

Effect of chronic low-level cadmium intoxication on the Haversian remodeling system in dogs. 慢性低水平镉中毒对犬Haversian重塑系统的影响。

Calcified Tissue Research Pub Date : 1978-12-08 DOI: 10.1007/BF02013249

C Anderson, K D Danylchuk

引用次数: 5

Correlation of freeze-fracture and scanning electron microscopy of epiphyseal chondrocytes. 骨骺软骨细胞冷冻骨折与扫描电镜的相关性。

Calcified Tissue Research Pub Date : 1978-12-08 DOI: 10.1007/BF02013264

T K Borg, R B Runyan, R E Wuthier

{"title":"Correlation of freeze-fracture and scanning electron microscopy of epiphyseal chondrocytes.","authors":"T K Borg, R B Runyan, R E Wuthier","doi":"10.1007/BF02013264","DOIUrl":"https://doi.org/10.1007/BF02013264","url":null,"abstract":"Chondrocytes in epiphyseal cartilage were examined by scanning electron microscopy (SEM) and transmission electron microscopy (TEM) using freeze-fracture techniques. Freeze-fracture replicas showed large numbers of fingerlike, 0.11-0.15 micrometer diameter, projections from the chondrocyte surface, with numerous 95-180 A diameter intramembranous particles associated with both the cell membrane surface and these projections. With SEM, these cytoplasmic projections were also obvious, but appeared collapsed into clusters of globular-shaped projections on the surface of the chondrocytes. With freeze-fracture techniques, in which shrinkage artifacts were essentially eliminated, the cytoplasmic projections were often seen in intimate contact with the extracapsular matrix. However, with chondrocytes prepared by both SEM and conventional TEM, there was evidence of shrinkage, the cytoplasmic projections having little contact with the extracapsular matrix. These findings show that the cytoplasmic processes are not artifacts of tissue processing and provide morphological evidence in support of the hypothesis that matrix vesicles are of cellular origin.","PeriodicalId":9389,"journal":{"name":"Calcified Tissue Research","volume":"26 3","pages":"237-41"},"PeriodicalIF":0.0,"publicationDate":"1978-12-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/BF02013264","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"11952036","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 15