Calcified Tissue Research最新文献

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The effects of 1,25-dihydroxycholecalciferol on embryonic bone in vitro: a biochemical and histological study. 1,25-二羟基胆骨化醇对体外胚胎骨的影响:生化和组织学研究。
Calcified Tissue Research Pub Date : 1978-05-26 DOI: 10.1007/BF02010759
M P Herrmann-Erlee, P J Gaillard
{"title":"The effects of 1,25-dihydroxycholecalciferol on embryonic bone in vitro: a biochemical and histological study.","authors":"M P Herrmann-Erlee,&nbsp;P J Gaillard","doi":"10.1007/BF02010759","DOIUrl":"https://doi.org/10.1007/BF02010759","url":null,"abstract":"<p><p>Explants from embryonic rat and mouse calvaria were cultivated in the presence of different concentrations of 1, 25-dihydroxycholecalciferol (1, 25-(OH)2D3). The bone resorbing effects of the vitamin D3 metabolite were evaluated by measuring the release of calcium, phosphate, lactate and citrate into the culture medium after 24 h of cultivation. The influence on bone morphology was studied using embryonic mouse radii, in which histological phenomena in the bony, cartilagenous and connective tissue compartments were observed. Both kinds of experiments show that 1, 25-(OH)2D3 has effects on embryonic bone which are typical for high concentrations of parathyroid hormone (PTH). However, the maximal effects on calcium release and on histology are 2.5 times less than those of PTH. In addition, some of the histological features such as the effects on epiphyseal cartilage and on osteoclasts were not observed. 1, 25-(OH)2D3 in concentrations up to 2 X 10(-9) M does not affect basal or PTH-stimulated cAMP levels in embryonic rat calvaria. In a concentration of 1 X 10(-8) M, however, a significant decrease in PTH-stimulated cAMP production was found. It is concluded from these in vitro experiments that (1) 1, 25-(OH)2D3 stimulates bone resorption, and (2) in 1, 25-(OH)2D3-induced bone resorption cAMP is apparently not involved as a second messenger.</p>","PeriodicalId":9389,"journal":{"name":"Calcified Tissue Research","volume":"25 2","pages":"111-8"},"PeriodicalIF":0.0,"publicationDate":"1978-05-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/BF02010759","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"11424549","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 10
Radiation induced free radicals as molecular probes in synthetic apatites. 辐射诱导自由基作为分子探针在合成磷灰石中的应用。
Calcified Tissue Research Pub Date : 1978-05-26 DOI: 10.1007/BF02010767
R A Peckauskas, I Pullman
{"title":"Radiation induced free radicals as molecular probes in synthetic apatites.","authors":"R A Peckauskas,&nbsp;I Pullman","doi":"10.1007/BF02010767","DOIUrl":"https://doi.org/10.1007/BF02010767","url":null,"abstract":"<p><p>Free radicals generated in synthetic apatitic calcium phosphates by X-ray radiation were investigated by electron spin resonance (ESR) spectroscopy. Among the species stable enough at -188 degrees C to be identified were hydrogen atoms, phosphate radicals, and oxygen anion radicals. The ESR spectra were markedly dependent on the specific surface of the mineral. Oxygen radicals dominated the spectra of low specific surface samples while phosphate radicals were the predominant species at higher specific surfaces. Our studies suggest that the oxygen radicals are more stable in the bulk of the crystal while the hydrogen atoms and the phosphate radicals are stabilized at or near the crystal surface. It was concluded that the surface species are potentially capable of serving as probes of biologically relevant mineral-organic interfaces.</p>","PeriodicalId":9389,"journal":{"name":"Calcified Tissue Research","volume":"25 2","pages":"191-5"},"PeriodicalIF":0.0,"publicationDate":"1978-05-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/BF02010767","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"11424551","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 6
The interaction of collagenase with hydroxyapatite and related materials and enzymatic properties of the adsorbed enzyme. 胶原酶与羟基磷灰石及相关物质的相互作用及吸附酶的酶学性质。
Calcified Tissue Research Pub Date : 1978-05-26 DOI: 10.1007/BF02010761
M Knuuttila, K U Paunio
{"title":"The interaction of collagenase with hydroxyapatite and related materials and enzymatic properties of the adsorbed enzyme.","authors":"M Knuuttila,&nbsp;K U Paunio","doi":"10.1007/BF02010761","DOIUrl":"https://doi.org/10.1007/BF02010761","url":null,"abstract":"<p><p>The commercial collagenase from Clostridium histolyticum was adsorbed on hydroxyapatite, on bovine femur shaft and on enamel and dentin powders. The substrate specificity of the adsorbed enzyme tested, with chromophore substrate, azocoll and native collagen, differed from that obtained with the soluble enzyme. The adsorption and the substrate specificity was also dependent on the adsorbent used. A pretreatment of hydroxyapatite with chondroitin sulphate, hyaluronate and DNA lowered the adsorption of collagenase. Phosphate ion caused desorption of the enzyme from hydroxyapatite. Sodium fluoride caused partial desorption of the enzyme from hydroxyapatite and enamel and dentin powders. Colangenase adsorbed on root surfaces of teeth liberated hydroxyproline containing material.</p>","PeriodicalId":9389,"journal":{"name":"Calcified Tissue Research","volume":"25 2","pages":"127-31"},"PeriodicalIF":0.0,"publicationDate":"1978-05-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/BF02010761","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"11424550","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
The fine structure of decalcified cartilage and bone: a comparison between decalcification procedures performed before and after embedding. 脱钙软骨和骨的精细结构:包埋前后脱钙程序的比较。
Calcified Tissue Research Pub Date : 1978-05-26 DOI: 10.1007/BF02010766
E Bonucci, J Reurink
{"title":"The fine structure of decalcified cartilage and bone: a comparison between decalcification procedures performed before and after embedding.","authors":"E Bonucci,&nbsp;J Reurink","doi":"10.1007/BF02010766","DOIUrl":"https://doi.org/10.1007/BF02010766","url":null,"abstract":"","PeriodicalId":9389,"journal":{"name":"Calcified Tissue Research","volume":"25 2","pages":"179-90"},"PeriodicalIF":0.0,"publicationDate":"1978-05-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/BF02010766","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"11562437","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 26
Metacarpal morphometry in monozygotic dizygotic elderly twins. 老年同卵异卵双胞胎掌骨形态测定。
Calcified Tissue Research Pub Date : 1978-05-26 DOI: 10.1007/BF02010768
M Møller, A Horsman, B Harvald, M Hauge, K Henningsen, B E Nordin
{"title":"Metacarpal morphometry in monozygotic dizygotic elderly twins.","authors":"M Møller,&nbsp;A Horsman,&nbsp;B Harvald,&nbsp;M Hauge,&nbsp;K Henningsen,&nbsp;B E Nordin","doi":"10.1007/BF02010768","DOIUrl":"https://doi.org/10.1007/BF02010768","url":null,"abstract":"<p><p>The relative importance of genetic factors in the pathogenesis of age related bone loss has been investigated in a study involving 17 monozygotic (MZ) and 8 dizygotic (DZ) pairs of twins aged 64 to 75 years. Radiographic morphometry was performed at the midpoints of the 2nd, 3rd and 4th metacarpals of both hands and the mean total and cortical widths were evaluated. The heritability, h2, was calculated as the difference between the intrapair variances in same sexed DZ and MZ pairs divided by the intrapair variance in DZ pairs. The mean intrapair variance of both total and cortical width was found to be four to five times higher in DZ than in MZ pairs. The differences are highly significant with an h2 value between 0.7 and 0.8, indicating a predominant genetic influence. It is stressed that this result applies only to the population from which the twin sample was drawn.</p>","PeriodicalId":9389,"journal":{"name":"Calcified Tissue Research","volume":"25 2","pages":"197-201"},"PeriodicalIF":0.0,"publicationDate":"1978-05-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/BF02010768","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"11770783","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 21
Paramagnetic and crystallographic effects of low temperature ashing on human bone and tooth enamel. 低温灰化对人体骨骼和牙釉质的顺磁性和晶体学影响。
Calcified Tissue Research Pub Date : 1978-02-28 DOI: 10.1007/BF02010757
H J Tochon-Danguy, J M Very, M Geoffroy, C A Baud
{"title":"Paramagnetic and crystallographic effects of low temperature ashing on human bone and tooth enamel.","authors":"H J Tochon-Danguy,&nbsp;J M Very,&nbsp;M Geoffroy,&nbsp;C A Baud","doi":"10.1007/BF02010757","DOIUrl":"https://doi.org/10.1007/BF02010757","url":null,"abstract":"<p><p>Low temperature ashing by excited gas (LTA) causes crystallographic and paramagnetic alterations of the human bone and tooth enamel mineral. On the one hand, LTA induces variations of the alpha lattice parameter. These variations depend upon the nature of the gas used, but are little affected by its degree of excitation. Trapping of gas molecules in the crystal structure is demonstrated. On the other hand, LTA produces two preponderant paramagnetic centers in bone and enamel samples at 20 degrees C. Their inorganic origin clearly indicated. One of the two radicals has been identified as O3- (g1 = 2.002, g2 = 2.010, g3 = 2.016) and the other as (CO3-3 (parallel = 1.996, g = perpendicular 2.003). Variations of the alpha lattice parameter and trapping of paramagnetic gas species do not seem to be directly related.</p>","PeriodicalId":9389,"journal":{"name":"Calcified Tissue Research","volume":"25 1","pages":"99-104"},"PeriodicalIF":0.0,"publicationDate":"1978-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/BF02010757","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"11422011","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 7
The intestinal handling of calcium by the rat in vivo, as affected by cortisol. Effect of dietary calcium supplements. 体内大鼠对钙的肠道处理,受皮质醇的影响膳食钙补充剂的作用。
Calcified Tissue Research Pub Date : 1978-02-28 DOI: 10.1007/BF02010743
J L Ferretti, J L Bazán, D Alloatti, R C Puche
{"title":"The intestinal handling of calcium by the rat in vivo, as affected by cortisol. Effect of dietary calcium supplements.","authors":"J L Ferretti,&nbsp;J L Bazán,&nbsp;D Alloatti,&nbsp;R C Puche","doi":"10.1007/BF02010743","DOIUrl":"https://doi.org/10.1007/BF02010743","url":null,"abstract":"","PeriodicalId":9389,"journal":{"name":"Calcified Tissue Research","volume":"25 1","pages":"1-6"},"PeriodicalIF":0.0,"publicationDate":"1978-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/BF02010743","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"11849576","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 8
Anticonvulsant osteomalacia induced in the rat by diphenylhydantoin. 二苯基苯妥英致大鼠抗惊厥性骨软化。
Calcified Tissue Research Pub Date : 1978-02-28 DOI: 10.1007/BF02010745
M Harris, D J Rowe, A J Darby
{"title":"Anticonvulsant osteomalacia induced in the rat by diphenylhydantoin.","authors":"M Harris,&nbsp;D J Rowe,&nbsp;A J Darby","doi":"10.1007/BF02010745","DOIUrl":"https://doi.org/10.1007/BF02010745","url":null,"abstract":"","PeriodicalId":9389,"journal":{"name":"Calcified Tissue Research","volume":"25 1","pages":"13-7"},"PeriodicalIF":0.0,"publicationDate":"1978-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/BF02010745","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"11519253","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 7
Purification of alkaline phosphatase from extracellular vesicles of fracture callus cartilage. 骨折骨痂软骨细胞外囊泡碱性磷酸酶的纯化。
Calcified Tissue Research Pub Date : 1978-02-28 DOI: 10.1007/BF02010755
S E Kahn, A M Jafri, N J Lewis, C Arsenis
{"title":"Purification of alkaline phosphatase from extracellular vesicles of fracture callus cartilage.","authors":"S E Kahn,&nbsp;A M Jafri,&nbsp;N J Lewis,&nbsp;C Arsenis","doi":"10.1007/BF02010755","DOIUrl":"https://doi.org/10.1007/BF02010755","url":null,"abstract":"","PeriodicalId":9389,"journal":{"name":"Calcified Tissue Research","volume":"25 1","pages":"85-92"},"PeriodicalIF":0.0,"publicationDate":"1978-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/BF02010755","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"11849420","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 13
The effect of electric fields on osteoporosis of disuse. 电场对废用性骨质疏松的影响。
Calcified Tissue Research Pub Date : 1978-02-28 DOI: 10.1007/BF02010747
R B Martin, W Gutman
{"title":"The effect of electric fields on osteoporosis of disuse.","authors":"R B Martin,&nbsp;W Gutman","doi":"10.1007/BF02010747","DOIUrl":"https://doi.org/10.1007/BF02010747","url":null,"abstract":"<p><p>An experiment originally done by McElhaney et al. was repeated to obtain additional information about the effects of electrical fields on osteoporosis of disuse. The right femurs of 35 male rats were immobilized in plaster casts. Sixteen rats were treated with transcutaneous electrical fields of 30 Hz and approximately 100 V/cm for periods of 2 or 8 h a day. While the right femurs of the untreated rats were found to be atrophic with respect to the opposite limb, in the treated rats the immobilized femur was made larger than the opposite bone. Longer daily treatments exaggerated this effect. The tumors found in the previous study were not seen in our experiments. Other similarities and differences in the 2 studies are discussed.</p>","PeriodicalId":9389,"journal":{"name":"Calcified Tissue Research","volume":"25 1","pages":"23-7"},"PeriodicalIF":0.0,"publicationDate":"1978-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/BF02010747","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"11519254","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 13
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