Biochimie最新文献_第7页

Assessment of membrane labelling mechanisms with exogenous fatty acids and detergents in bacteria. 用外源脂肪酸和洗涤剂评估细菌的膜标记机制。

Biochimie Pub Date : 2024-12-01 Epub Date: 2024-05-31 DOI: 10.1016/j.biochi.2024.05.024

Laila Zaatouf, Kiran Kumar, Isabelle Marcotte, Dror E Warschawski

{"title":"Assessment of membrane labelling mechanisms with exogenous fatty acids and detergents in bacteria.","authors":"Laila Zaatouf, Kiran Kumar, Isabelle Marcotte, Dror E Warschawski","doi":"10.1016/j.biochi.2024.05.024","DOIUrl":"10.1016/j.biochi.2024.05.024","url":null,"abstract":"Labelling of bacterial membranes using exogenous fatty acids has proven to be a valuable tool to investigate molecular interactions by in-cell solid-state nuclear magnetic resonance (ssNMR) spectroscopy, notably with antimicrobial peptides. However, the mechanism by which this labelling takes place in non-mutated bacteria has not yet been investigated. In this work, we propose a rapid method to assess the fate of the fatty acids during the labelling of bacteria, involving two different methylation schemes and gas chromatography coupled to mass spectrometry. We applied this approach to Gram(+) and Gram(-) bacteria grown with deuterated palmitic acid under different conditions. We assessed the extent of labelling, then the resulting membrane rigidity by 2H ssNMR. Our results reveal that the labelling mechanism depends on the detergent used to micellize the fatty acids. This labelling can be either active or passive, whether the fatty acids are metabolized and used in the phospholipids biosynthesis, or remain unmodified in the membrane. We discuss the best labelling protocol for studying peptide-membrane interactions.","PeriodicalId":93898,"journal":{"name":"Biochimie","volume":" ","pages":"12-18"},"PeriodicalIF":0.0,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141201583","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Tailor-made vincristine-liposomes for tumor targeting. "用于肿瘤靶向的定制长春新碱脂质体"。

Biochimie Pub Date : 2024-12-01 Epub Date: 2024-07-31 DOI: 10.1016/j.biochi.2024.07.017

Ariana Abawi, Ana-Maria Trunfio-Sfarghiu, Céline Thomann, Emma Petiot, Giovanna Lollo, Thierry Granjon, Agnès Girard-Egrot, Ofelia Maniti

引用次数: 0

Glucose concentration is determinant for the functioning of hydrogenase 1 and hydrogenase 2 in regulating the proton and potassium fluxes in Escherichia coli at pH 7.5. 在 pH 值为 7.5 的大肠杆菌中，葡萄糖浓度对氢化酶 1 和氢化酶 2 调节质子和钾通量的功能起着决定性作用。

Biochimie Pub Date : 2024-12-01 Epub Date: 2024-07-20 DOI: 10.1016/j.biochi.2024.07.013

Liana Vanyan, Karen Trchounian

{"title":"Glucose concentration is determinant for the functioning of hydrogenase 1 and hydrogenase 2 in regulating the proton and potassium fluxes in Escherichia coli at pH 7.5.","authors":"Liana Vanyan, Karen Trchounian","doi":"10.1016/j.biochi.2024.07.013","DOIUrl":"10.1016/j.biochi.2024.07.013","url":null,"abstract":"This study examines how FOF1-ATPase, hydrogenases (Hyd-1 and Hyd-2), and potassium transport systems (TrkA) interact to maintain the proton motive force (pmf) in E. coli during fermentation of different glucose concentrations (2 g L-1 and 8 g L-1). Our findings indicate that mutants lacking the hyaA-hyaC genes exhibited a 30 % increase in total proton flux compared to the wild type when grown with 2 g L-1 glucose. This has been observed during assays where similar glucose levels were supplemented. Disruptions in proton pumping, particularly in hyaB and hyaC single mutants, led to increased potassium uptake. The hyaB mutant showed a threefold increase in the contribution of FOF1-ATPase to proton flux, suggesting a significant role for Hyd-1 in proton translocation. In the hybC mutant grown in 2 g L-1 glucose conditions, DCCD-sensitive fluxes decreased by 70 %, indicating critical role of Hyd-2 in proton transport and FOF1 function. When cells were grown with 8 g L-1 glucose, the 2H+/1K+ ratio was significantly disturbed in both wild type and mutants. Despite these perturbances, mutants with disruptions in Hyd-1 and Hyd-2 maintained constant FOF1 function, suggesting that this enzyme remains stable in glucose-rich environments. These results provide valuable insights into how Hyd-1 and Hyd-2 contribute to the regulation of ion transport, particularly proton translocation, in response to glucose concentration. Our study uncovered potential complementary mechanisms between Hyd-1 and Hyd-2 subunits, suggesting a complex interplay between these enzymes via metabolic cross talk with FOF1 in response to glucose concentrations to maintain pmf.","PeriodicalId":93898,"journal":{"name":"Biochimie","volume":" ","pages":"205-216"},"PeriodicalIF":0.0,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141749997","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Lipids, from molecular mechanisms to diseases. 脂质，从分子机制到疾病。

Biochimie Pub Date : 2024-12-01 Epub Date: 2024-11-12 DOI: 10.1016/j.biochi.2024.11.001

Isabelle Dugail, Sylvie Demignot, Lhousseine Touqui, Abdou Rachid Thiam

引用次数: 0

Established and emerging players in phospholipid scrambling: A structural perspective. 磷脂争夺中的成熟和新兴参与者：结构视角。

Biochimie Pub Date : 2024-12-01 Epub Date: 2024-09-18 DOI: 10.1016/j.biochi.2024.09.008

Heitor Gobbi Sebinelli, Camille Syska, Alenka Čopič, Guillaume Lenoir

{"title":"Established and emerging players in phospholipid scrambling: A structural perspective.","authors":"Heitor Gobbi Sebinelli, Camille Syska, Alenka Čopič, Guillaume Lenoir","doi":"10.1016/j.biochi.2024.09.008","DOIUrl":"10.1016/j.biochi.2024.09.008","url":null,"abstract":"The maintenance of a diverse and non-homogeneous lipid composition in cell membranes is crucial for a multitude of cellular processes. One important example is transbilayer lipid asymmetry, which refers to a difference in lipid composition between the two leaflets of a cellular membrane. Transbilayer asymmetry is especially pronounced at the plasma membrane, where at resting state, negatively-charged phospholipids such as phosphatidylserine (PS) are almost exclusively restricted to the cytosolic leaflet, whereas sphingolipids are mostly found in the exoplasmic leaflet. Transbilayer movement of lipids is inherently slow, and for a fast cellular response, for example during apoptosis, transmembrane proteins termed scramblases facilitate the movement of polar/charged lipid headgroups through the membrane interior. In recent years, an expanding number of proteins from diverse families have been suggested to possess a lipid scramblase activity. Members of TMEM16 and XKR proteins have been implicated in blood clotting and apoptosis, whereas the scrambling activity of ATG9 and TMEM41B/VMP1 proteins contributes to the synthesis of autophagosomal membrane during autophagy. Structural studies, in vitro reconstitution of lipid scrambling, and molecular dynamics simulations have significantly advanced our understanding of the molecular mechanisms of lipid scrambling and helped delineate potential lipid transport pathways through the membrane. A number of examples also suggest that lipid scrambling activity can be combined with another activity, as is the case for TMEM16 proteins, which also function as ion channels, rhodopsin in the photoreceptor membrane, and possibly other G-protein coupled receptors.","PeriodicalId":93898,"journal":{"name":"Biochimie","volume":" ","pages":"111-122"},"PeriodicalIF":0.0,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142303292","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Combined therapeutic use of umbilical cord blood serum and amniotic membrane in diabetic wounds. 脐带血血清和羊膜在糖尿病伤口中的联合治疗应用。

Biochimie Pub Date : 2024-12-01 Epub Date: 2024-07-21 DOI: 10.1016/j.biochi.2024.07.012

C Montague, Y Holt, M Vlok, P Dhanraj, K Boodhoo, M Maartens, K Buthelezi, C U Niesler, M van de Vyver

{"title":"Combined therapeutic use of umbilical cord blood serum and amniotic membrane in diabetic wounds.","authors":"C Montague, Y Holt, M Vlok, P Dhanraj, K Boodhoo, M Maartens, K Buthelezi, C U Niesler, M van de Vyver","doi":"10.1016/j.biochi.2024.07.012","DOIUrl":"10.1016/j.biochi.2024.07.012","url":null,"abstract":"Diabetic wounds are hard-to-heal due to complex multifactorial dysregulation within the micro-environment, necessitating the development of novel regenerative approaches to stimulate healing. This study investigated whether the combined therapeutic application of two novel cellular tissue products, namely a decellularized collagen-rich amniotic membrane (AmR) and growth factor-rich umbilical cord blood serum (UCBS) could have a positive synergistic effect on long-term healing outcomes by stimulating both superficial wound closure and wound bed regeneration. Full thickness excisional wounds were induced on obese diabetic mice (B6.Cg-lepob/J, ob/ob, n = 23) and treated with either: 1) Standard wound care (control); 2) UCBS; 3) AmR or 4) UCBS + AmR. Macroscopic wound closure was assessed on days 0, 3, 7, 10 and 14 post wounding. To determine the potential impact on wound recurrence, endpoint analysis was performed to determine both the overall quality of healing histologically as well as the molecular state of the wounds on day 14 via proteomic analysis. The data demonstrated the presence of both healers and non-healers. Re-epithelization took place in the healers of all treatment groups, but underlying tissue regeneration was far more pronounced following application of the combined treatment (UCBS + AmR), suggesting improved quality of healing and potentially a reduced change of recurrence long term. In non-healers, wounds failed to heal due to excessive slough formation and a reduction in LTB4 expression, suggesting impaired antimicrobial activity. Care should thus be taken since the cellular tissue product therapy could pose an increased risk for infection in some patients.","PeriodicalId":93898,"journal":{"name":"Biochimie","volume":" ","pages":"193-204"},"PeriodicalIF":0.0,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141753551","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Infection by zika virus increase angiotensin I-converting enzyme activity in mouse brain. 寨卡病毒感染小鼠脑内血管紧张素 I 转换酶活性增加：对治疗的影响？

Biochimie Pub Date : 2024-12-01 Epub Date: 2024-07-02 DOI: 10.1016/j.biochi.2024.07.001

William Y Oyadomari, Raissa R Christoff, João V Nani, Tailene Rabello, Vitor Oliveira, Luiza M Higa, Patrícia P Garcez, Mirian A F Hayashi

{"title":"Infection by zika virus increase angiotensin I-converting enzyme activity in mouse brain.","authors":"William Y Oyadomari, Raissa R Christoff, João V Nani, Tailene Rabello, Vitor Oliveira, Luiza M Higa, Patrícia P Garcez, Mirian A F Hayashi","doi":"10.1016/j.biochi.2024.07.001","DOIUrl":"10.1016/j.biochi.2024.07.001","url":null,"abstract":"Congenital zika virus syndrome (CZS) has become a significant worldwide concern since the sudden rise of microcephaly related to zika virus (ZIKV) in Brazil. Primarily transmitted by Aedes mosquitoes, ZIKV shares serologic similarities with dengue virus (DENV), complicating the diagnosis and/or clinical management. The Angiotensin I-Converting Enzyme (ACE) was associated with either neuroprotective or anti-inflammatory properties in the central nervous system (CNS). The possible role(s) of ACE in these two flaviviruses infection remain largely unexplored. In this study, we evaluate ACE activity in the brain of ZIKV- or DENV-infected mice, both compared to MOCK, showing about 30 % increased ACE activity only in ZIKV-infected mice (p = 0.024), while no change was noticed in brain from DENV-infected animals (p = 0.888). In addition, the treatment with interferon beta (IFNβ), under conditions previously demonstrated to rescue the normal size of microcephalic brains determined by ZIKV infection, also restored ACE activity in ZIKV-infected animals to levels close to that of the MOCK control group. Although inflammatory responses expected for either ZIKV or DENV infections, only ZIKV was associated with microcephaly, as well as with increased ACE activity and reversion by treatment with IFNβ. Furthermore, this increase in ACE activity was observed only after intracerebroventricular (ICV) injection (F (2, 16) = 7.907, p = 0.004), but not for intraperitoneal (IP) administration of ZIKV (F (2, 26) = 1.996, p = 0.156), suggesting that the observed central ACE activity modulation may be associated with the presence of this specific flavivirus in the brain.","PeriodicalId":93898,"journal":{"name":"Biochimie","volume":" ","pages":"116-118"},"PeriodicalIF":0.0,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141499885","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Characterization of the Arabidopsis thaliana chromatin remodeler DEK3 for its interaction with histones and DNA. 拟南芥染色质重塑器 DEK3 与组蛋白和 DNA 相互作用的特征。

Biochimie Pub Date : 2024-12-01 Epub Date: 2024-08-06 DOI: 10.1016/j.biochi.2024.07.018

Rajivgandhi Sundaram, Surajit Gandhi, Claudia Jonak, Dileep Vasudevan

{"title":"Characterization of the Arabidopsis thaliana chromatin remodeler DEK3 for its interaction with histones and DNA.","authors":"Rajivgandhi Sundaram, Surajit Gandhi, Claudia Jonak, Dileep Vasudevan","doi":"10.1016/j.biochi.2024.07.018","DOIUrl":"10.1016/j.biochi.2024.07.018","url":null,"abstract":"Chromatin structure and dynamics regulate all DNA-templated processes, such as transcription, replication, and repair. Chromatin binding factors, chromatin architectural proteins, and nucleosome remodelers modulate chromatin structure and dynamics and, thereby, the various DNA-dependent processes. Arabidopsis thaliana DEK3, a member of the evolutionarily conserved DEK domain-containing chromatin architectural proteins, is an important factor for chromatin structure and function, involved in transcriptional programming to regulate flowering time and abiotic stress tolerance. AtDEK3 contains an uncharacterized N-terminal domain, a middle SAF domain (winged helix-like domain), and a C-terminal DEK domain, but their role in the interaction of AtDEK3 with histones and DNA remained poorly understood. Using biochemical and biophysical analyses, we provide a comprehensive in vitro characterization of the different AtDEK3 domains for their interaction with histone H3/H4 and DNA. AtDEK3 directly interacts with histone H3/H4 tetramers through its N-terminal domain and the C-terminal DEK domain in a 1:1 stoichiometry. Upon interaction with H3/H4, the unstructured N-terminal domain of AtDEK3 undergoes a conformational change and adopts an alpha-helical conformation. In addition, the in-solution envelope structures of the AtDEK3 domains and their complex with H3/H4 have been characterized. The SAF and DEK domains associate with double-stranded and four-way junction DNA. As DEK3 possesses a histone-interacting domain at the N- and the C-terminus and a DNA-binding domain in the middle and at the C-terminus, the protein might play a complex role as a chromatin remodeler.","PeriodicalId":93898,"journal":{"name":"Biochimie","volume":" ","pages":"248-261"},"PeriodicalIF":0.0,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141891255","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A new isoxazolyl-urea derivative induces apoptosis, paraptosis, and ferroptosis by modulating MAPKs in pancreatic cancer cells. 一种新的异噁唑基脲衍生物通过调节胰腺癌细胞中的 MAPKs，诱导细胞凋亡、副凋亡和铁凋亡。

Biochimie Pub Date : 2024-12-01 Epub Date: 2024-08-02 DOI: 10.1016/j.biochi.2024.08.001

Young Yun Jung, Rajaghatta N Suresh, Chakrabhavi Dhananjaya Mohan, Kachigere B Harsha, Chilkunda Sannaiah Shivakumara, Kanchugarakoppal S Rangappa, Kwang Seok Ahn

{"title":"A new isoxazolyl-urea derivative induces apoptosis, paraptosis, and ferroptosis by modulating MAPKs in pancreatic cancer cells.","authors":"Young Yun Jung, Rajaghatta N Suresh, Chakrabhavi Dhananjaya Mohan, Kachigere B Harsha, Chilkunda Sannaiah Shivakumara, Kanchugarakoppal S Rangappa, Kwang Seok Ahn","doi":"10.1016/j.biochi.2024.08.001","DOIUrl":"10.1016/j.biochi.2024.08.001","url":null,"abstract":"MAPK pathway regulates the major events including cell division, cell death, migration, invasion, and angiogenesis. Small molecules that modulate the MAPK pathway have been demonstrated to impart cytotoxicity in cancer cells. Herein, the synthesis of a new isoxazolyl-urea derivative (QR-4) has been described and its effect on the growth of pancreatic cancer cells has been investigated. QR-4 reduced the cell viability in a panel of pancreatic cancer cells with minimal effect on normal hepatocytes. QR-4 induced the cleavage of PARP and procaspase-3, reduced the expression of antiapoptotic proteins, increased SubG1 cells, and annexin V/PI-stained cells indicating the induction of apoptosis. QR-4 also triggered paraptosis as witnessed by the reduction of mitochondrial membrane potential, decrease in the expression of Alix, increase in the levels of ATF4 and CHOP, and enhanced ER stress. QR-4 also modulated ferroptosis-related events such as elevation in iron levels, alteration in GSH/GSSG ratio, and increase in the expression of TFRC with a parallel decrease in the expression of GPX4 and SLC7A11. The mechanistic approach revealed that QR-4 increases the phosphorylation of all three forms of MAPKs (JNK, p38, and ERK). Independent application of specific inhibitors of these MAPKs resulted in a partial reversal of QR-4-induced effects. Overall, these reports suggest that a new isoxazolyl-urea imparts cell death via apoptosis, paraptosis, and ferroptosis by regulating the MAPK pathway in pancreatic cancer cells.","PeriodicalId":93898,"journal":{"name":"Biochimie","volume":" ","pages":"262-272"},"PeriodicalIF":0.0,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141891254","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Polyamines putrescine and spermidine as modulators of protein aggregation rate: The effect on DTT-induced aggregation of α-lactalbumin. 作为蛋白质聚集率调节剂的多胺--腐胺和亚精胺：对 DTT 诱导的 α-乳清蛋白聚集的影响。

Biochimie Pub Date : 2024-12-01 Epub Date: 2024-07-20 DOI: 10.1016/j.biochi.2024.07.011

Dmitriy A Kara, Vera A Borzova, Svetlana G Roman, Sergey Yu Kleymenov, Natalia A Chebotareva

{"title":"Polyamines putrescine and spermidine as modulators of protein aggregation rate: The effect on DTT-induced aggregation of α-lactalbumin.","authors":"Dmitriy A Kara, Vera A Borzova, Svetlana G Roman, Sergey Yu Kleymenov, Natalia A Chebotareva","doi":"10.1016/j.biochi.2024.07.011","DOIUrl":"10.1016/j.biochi.2024.07.011","url":null,"abstract":"Protein aggregation is undesirable for cells due to its possible toxicity, and is also undesirable in biotechnology and pharmaceuticals. Polyamines are known to be capable of both suppressing and stimulating protein aggregation. In the present work polyamines (spermidine, putrescine) have been shown to alter the pathway of α-lactalbumin aggregation induced by dithiothreitol, leading to the formation of larger protein particles during the initial stages of aggregation and promoting the later stage of sticking of aggregates. According to the aggregation kinetics data, polyamines accelerate protein aggregation in a concentration-dependent manner, with a maximum at 50 mM spermidine and 100 mM putrescine. With a further increase in polyamines concentration the effect of aggregation acceleration decreased, thus, the modulation of the aggregation rate by polyamines was shown. A comparison of the aggregation kinetics and hydrodynamic radii growth data registered by dynamic light scattering with the data obtained by asymmetric flow field-flow fractionation and analytical ultracentrifugation allowed us to describe the early stages of aggregation and formation of initial α-lactalbumin clusters. Our results provide a deeper insight into the mechanism of amorphous aggregation of α-lactalbumin and polyamines action on protein aggregation and protein-protein interaction in general.","PeriodicalId":93898,"journal":{"name":"Biochimie","volume":" ","pages":"182-192"},"PeriodicalIF":0.0,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141735980","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0