IF 3

Biochimie Pub Date : 2025-10-06 DOI: 10.1016/j.biochi.2025.10.001

Aditi Pathak, Ramanathan Sowdhamini

{"title":"Computational structural analysis sheds light on the molecular grammar of gap junction proteins.","authors":"Aditi Pathak, Ramanathan Sowdhamini","doi":"10.1016/j.biochi.2025.10.001","DOIUrl":"https://doi.org/10.1016/j.biochi.2025.10.001","url":null,"abstract":"Gap junctions are multimeric intercellular channels that permit ions and small molecules to pass directly from one cell to another. Despite being fundamental to multicellular life, these channels are formed by distantly related protein families: innexins in invertebrates and connexins in vertebrates. Vertebrates also express pannexins, distant homologues of primordial innexins, which only form hemichannels. While these families have diverse sequences and different oligomeric states, their monomeric structures are highly similar. We generated structure-guided sequence alignments to establish equivalent residues across innexins, connexins, and pannexins. Further, computational approaches for determining protein-protein interaction hotspots, residue conservation, accessible surface area and local conformations of residues, provide insights into the relationships between residue positions and channel properties. We find that exposed transmembrane residues of TM1 and TM2 are more conserved than those in TM3 and TM4, especially in connexins and pannexins. Moreover, we see that residues in the extracellular extended hairpins of pannexins show more conformational flexibility, in variable protein blocks, than equivalent residues in connexins. This hints that the rigidity of this element could be a prerequisite for hemichannel docking. Finally, we identify inter- and intra-hemichannel interface hotspots that are positionally conserved across the families, implying their role in hemichannel and, ultimately, gap junction formation. Such analyses reveal a molecular grammar that underlies gap junction design and offer a basis for targeted perturbation of channel properties.","PeriodicalId":93898,"journal":{"name":"Biochimie","volume":" ","pages":""},"PeriodicalIF":3.0,"publicationDate":"2025-10-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145254148","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Altered hemostatic dynamics and its regulation in follicular microenvironment of women with polycystic ovary syndrome. 多囊卵巢综合征妇女的止血动力学改变及其对卵泡微环境的调节。

IF 3

Biochimie Pub Date : 2025-09-27 DOI: 10.1016/j.biochi.2025.09.015

Roshan Dadachanji, Snehal Bhingardeve, Sadhana K Desai, Vijay Mangoli, Srabani Mukherjee

{"title":"Altered hemostatic dynamics and its regulation in follicular microenvironment of women with polycystic ovary syndrome.","authors":"Roshan Dadachanji, Snehal Bhingardeve, Sadhana K Desai, Vijay Mangoli, Srabani Mukherjee","doi":"10.1016/j.biochi.2025.09.015","DOIUrl":"https://doi.org/10.1016/j.biochi.2025.09.015","url":null,"abstract":"Polycystic ovary syndrome (PCOS), presents with gynecological and metabolic issues such as anovulatory infertility, insulin resistance, hyperandrogenism, and obesity, and long-term cardiometabolic risks. Emerging evidence highlights coagulation-fibrinolysis balance influences essential ovarian functions, including ovulation, corpus luteum function, granulosa cell luteinization, and ECM remodeling. This study explores the relatively understudied coagulation and fibrinolytic factors in the ovarian microenvironment in Indian women. This case-control study examined the hemostatic potential of the follicular microenvironment in PCOS (n=35) and controls (n=30) by analyzing coagulation and fibrinolytic profiles in follicular fluid (FF), and granulosa cells (GCs). We observed significantly reduced transcript expressions of fibrinolytic factors including serine proteinase inhibitor (SERPINE1), tissue-type plasminogen activator (PLAT), coagulation factor fibrinogen gamma (FGG) in PCOS GCs. Conversely, transcript levels of thrombomodulin (THBD) and urokinase plasminogen activator receptor (PLAUR) were significantly higher in GCs of PCOS women compared to controls. Levels of both plasminogen activators, PLAT and PLAU, along with anticoagulant proteins, tissue factor pathway inhibitor and protein S were markedly declined, while plasminogen, THBD and histidine rich glycoprotein levels were significantly raised in FF of women with PCOS. Further, the construction of miRNA-mRNA regulatory network suggested that miRNAs may also be involved in hemostatic regulation in follicle microenvironment of PCOS. Our study showed that altered profiles of coagulation and fibrinolysis factors within follicular microenvironment of PCOS could contribute to disrupted hemostatic balance, mainly evidenced by compromised fibrinolysis. This may have significant implications for ovulatory dysfunction due to altered rupture, ECM remodeling and cumulus expansion in affected women.","PeriodicalId":93898,"journal":{"name":"Biochimie","volume":" ","pages":""},"PeriodicalIF":3.0,"publicationDate":"2025-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145194177","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Beneficial effects of long-chain n-3 fatty acids on insulin-resistance: basic and clinical aspects. 长链n-3脂肪酸对胰岛素抵抗的有益作用：基础和临床方面。

IF 3

Biochimie Pub Date : 2025-09-26 DOI: 10.1016/j.biochi.2025.09.014

Jacques Delarue

{"title":"Beneficial effects of long-chain n-3 fatty acids on insulin-resistance: basic and clinical aspects.","authors":"Jacques Delarue","doi":"10.1016/j.biochi.2025.09.014","DOIUrl":"https://doi.org/10.1016/j.biochi.2025.09.014","url":null,"abstract":"This review aims to provide a comprehensive overview of the available data on the effects of long-chain n-3 polyunsaturated fatty acids (LC n-3 PUFAs) on insulin resistance (IR) associated with non-communicable diseases (NCDs), including obesity and type 2 diabetes. In recent years, inflammation of adipose tissue (AT) has emerged as a pivotal contributor to IR in these NCDs. Basic studies conducted on isolated adipocytes and rodents consistently demonstrate that LC n-3 PUFAs attenuate AT inflammation through multiple mechanisms. Furthermore, rodent studies have shown that even low doses of LC n-3 PUFAs can effectively prevent IR, particularly when induced by a high-fat diet (HFD). However, trials conducted in humans, using primarily hyperinsulinemic clamp methods, have yielded more varied results, with several studies indicating a sensitising effect on liver and, occasionally, muscle (as assessed by plasma glucose utilisation). In patients with polycystic ovary syndrome (PCOS), gestational diabetes, and metabolic dysfunction-associated steatohepatitis (MASLD), LC n-3 PUFAs have been observed to decrease IR, as measured by the Homeostasis Model Assessment-Insulin Resistance (HOMA-IR) score. In contrast, most meta-analyses of studies conducted in patients with type 2 diabetes (T2D) have concluded to the inefficacy of LC n-3 PUFAs in reducing IR. However, several meta-analyses have identified a protective effect of LC n-3 PUFAs against T2D in Asians, with no heterogeneity observed, contrasting the findings in Western populations, where heterogeneity exists. The most recent analysis and the large UK Biobank cohort have concluded to a protective effect of LC n-3 PUFAs. Therefore, it can be proposed that LC n-3 PUFAs should be administered to individuals with NCD-associated IR and at high risk of T2D, in conjunction with a healthy diet, such as the Mediterranean diet.","PeriodicalId":93898,"journal":{"name":"Biochimie","volume":" ","pages":""},"PeriodicalIF":3.0,"publicationDate":"2025-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145187998","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The role of UCP5 in cardiac aging and metabolism in Drosophila. UCP5在果蝇心脏衰老和代谢中的作用。

IF 3

Biochimie Pub Date : 2025-09-24 DOI: 10.1016/j.biochi.2025.09.012

Yanxu Zhang, Yiyuan Zhai, Ying Bai, Qun Zeng, Min Tang

{"title":"The role of UCP5 in cardiac aging and metabolism in Drosophila.","authors":"Yanxu Zhang, Yiyuan Zhai, Ying Bai, Qun Zeng, Min Tang","doi":"10.1016/j.biochi.2025.09.012","DOIUrl":"10.1016/j.biochi.2025.09.012","url":null,"abstract":"Uncoupling proteins, as mitochondrial transporters, allow protons to enter the mitochondrial matrix without generating ATP, a process known as oxidative phosphorylation uncoupling. Mammalian UCPs have been demonstrated to regulate metabolism, modulate reactive oxygen species levels, and maintain calcium homeostasis, which is closely linked to cardiac disease. In Drosophila, four homologs of uncoupling protein have been identified, with only UCP5 being detected in the adult heart proteome by mass spectrometry. The essential role of Drosophila UCP5 in the heart remains unknown. Our results showed that cardiac-specific UCP5 overexpression increased the incidence of fibrillation in an age-dependent trend, while cardiac-specific UCP5 knockdown induced an age-dependent increase in the incidence of asystoles, likely due to tachycardia. Additionally, UCP5 RNA levels significantly decline with age, indicating a role of UCP5 in cardiac aging. Cardiac-specific UCP5 overexpression reduced the reactive oxygen species levels within the cardiomyocyte nuclei and extended the lifespan. UCP5 RNA levels increased under high-fat diet conditions, and systemic overexpression of UCP5 can lower triglyceride levels under such dietary conditions, indicating an adaptive role of UCP5 in metabolism.","PeriodicalId":93898,"journal":{"name":"Biochimie","volume":" ","pages":""},"PeriodicalIF":3.0,"publicationDate":"2025-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145180856","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

RNA at the Crossroads of Structure, Function, and Disease. RNA在结构、功能和疾病的十字路口。

IF 3

Biochimie Pub Date : 2025-09-24 DOI: 10.1016/j.biochi.2025.09.013

Didier Auboeuf, Cyril F Bourgeois, Francesca Fiorini, Virginie Marcel

引用次数: 0

2-Aminothiophene derivatives reduce resistance to fluoroquinolones in Staphylococcus aureus strains which overexpress NorA and MepA efflux pumps. 2-氨基噻吩衍生物降低了过表达NorA和MepA外排泵的金黄色葡萄球菌菌株对氟喹诺酮类药物的耐药性。

IF 3

Biochimie Pub Date : 2025-09-20 DOI: 10.1016/j.biochi.2025.09.001

Priscilla Augusta de Sousa Fernandes, Rodrigo Santos Aquino de Araújo, Gabriel Gonçalves Alencar, Sheila Alves Gonçalves, Gildênia Alves de Araújo, Ewerton Yago de Sousa Rodrigues, Daniel Sampaio Alves, Ray Silva de Almeida, Cícera Datiane de Morais Oliveira-Tintino, Maria Gabriella S Sidrônio, Valnês S Rodrigues, Emanuelly Karla Araújo Padilha, Edeildo Ferreira da Silva, Anuraj Nayarisseri, Teresinha Gonçalves da Silva, Henrique Douglas de Melo Coutinho, Francisco Jaime Bezerra Mendonça-Junior

{"title":"2-Aminothiophene derivatives reduce resistance to fluoroquinolones in Staphylococcus aureus strains which overexpress NorA and MepA efflux pumps.","authors":"Priscilla Augusta de Sousa Fernandes, Rodrigo Santos Aquino de Araújo, Gabriel Gonçalves Alencar, Sheila Alves Gonçalves, Gildênia Alves de Araújo, Ewerton Yago de Sousa Rodrigues, Daniel Sampaio Alves, Ray Silva de Almeida, Cícera Datiane de Morais Oliveira-Tintino, Maria Gabriella S Sidrônio, Valnês S Rodrigues, Emanuelly Karla Araújo Padilha, Edeildo Ferreira da Silva, Anuraj Nayarisseri, Teresinha Gonçalves da Silva, Henrique Douglas de Melo Coutinho, Francisco Jaime Bezerra Mendonça-Junior","doi":"10.1016/j.biochi.2025.09.001","DOIUrl":"10.1016/j.biochi.2025.09.001","url":null,"abstract":"In response to the growing threat of antibiotic resistance, this study aimed to identify novel compounds capable of modulating and/or even restoring antibiotic efficacy by inhibiting bacterial efflux pumps. Thirteen 2-aminothiophene (2-AT) derivatives were synthesized and tested against Staphylococcus aureus strains overexpressing NorA and MepA pumps, which confer resistance to fluoroquinolones. Although the 2-ATs displayed little inherent antibacterial activity, several-particularly compounds P4, P7, and P8-significantly potentiated the effects of norfloxacin, ciprofloxacin, and ethidium bromide (EtBr), reducing their Minimum Inhibitory Concentrations (MICs) by up to fourfold. P7 and P8, both 2-aminoselenophene bioisosteres, emerged as especially effective, demonstrating strong efflux pump inhibitory (EPI) activity and, for the first time, confirming their ability to inhibit MepA-mediated efflux in S. aureus. Cytotoxicity assays on VeroE6 and HepG2 cell lines confirmed the safety profile of selected compounds. EtBr accumulation assays and molecular dynamics simulations further supported the mechanism of action, confirming that these derivatives inhibit efflux activity. Overall, the results highlight the potential of 2-AT derivatives-especially P7 and P8-as promising EPIs to combat fluoroquinolone-resistant S. aureus.","PeriodicalId":93898,"journal":{"name":"Biochimie","volume":" ","pages":""},"PeriodicalIF":3.0,"publicationDate":"2025-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145126201","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Microbial degradation of hydrocarbons from petroleum assisted by biosurfactants: Pathways and bioremediation potential. 生物表面活性剂对石油烃类的微生物降解：途径和生物修复潜力。

IF 3

Biochimie Pub Date : 2025-09-19 DOI: 10.1016/j.biochi.2025.09.011

Jiaxin Cui, Peter Dörmann

{"title":"Microbial degradation of hydrocarbons from petroleum assisted by biosurfactants: Pathways and bioremediation potential.","authors":"Jiaxin Cui, Peter Dörmann","doi":"10.1016/j.biochi.2025.09.011","DOIUrl":"10.1016/j.biochi.2025.09.011","url":null,"abstract":"Petroleum pollution from oil extraction, transportation, and industrial activities poses significant threats to marine ecosystems and socioeconomic stability due to the high toxicity of alkanes and polycyclic aromatic hydrocarbons (PAHs) to many organisms. Traditional remediation methods, including physical removal and chemical oxidation, are often costly and ecologically disruptive. Microbial degradation, facilitated by hydrocarbonoclastic bacteria like Pseudomonas aeruginosa and Alcanivorax borkumensis, offers a sustainable alternative by converting hydrocarbons into non-toxic CO2 and water. This review examines the degradation pathways of aliphatic and aromatic hydrocarbons by these bacteria, highlighting key enzymatic mechanisms and the pivotal role of biosurfactants-specifically rhamnolipids, and glycine-glucolipid-in enhancing hydrocarbon bioavailability. It also delves into the biosynthesis of these biosurfactants, along with the involvement of non-ribosomal peptide synthetases (NRPS) in producing lipopeptide biosurfactants such as surfactin. Additionally, the review addresses the challenges associated with scaling up biosurfactant production for bioremediation applications. Through a synopsis of recent research, this work proposes strategies to optimize biosurfactant efficacy, contributing to environmental sustainability and advancing the field of microbial ecology.","PeriodicalId":93898,"journal":{"name":"Biochimie","volume":" ","pages":""},"PeriodicalIF":3.0,"publicationDate":"2025-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145115422","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Salmon and krill phospholipids: Two nanocarriers with interesting physico-chemical properties. 鲑鱼和磷虾磷脂：两种具有有趣物理化学性质的纳米载体。

IF 3

Biochimie Pub Date : 2025-09-18 DOI: 10.1016/j.biochi.2025.09.010

Maureen Gerlei, Louis Pruvost, Michel Linder

{"title":"Salmon and krill phospholipids: Two nanocarriers with interesting physico-chemical properties.","authors":"Maureen Gerlei, Louis Pruvost, Michel Linder","doi":"10.1016/j.biochi.2025.09.010","DOIUrl":"10.1016/j.biochi.2025.09.010","url":null,"abstract":"This study compares salmon heads and krill as natural marine sources of omega-3-rich phospholipids for liposome formulation, serving as potential carriers of bioactive compounds in nutrition and preventive health. Salmon and krill oil obtained by green processes, yielded lipid fractions enriched in both neutral and polar lipids, containing esterified long-chain polyunsaturated fatty acids (LC-PUFAs) such as EPA and DHA. The triacylglycerol/polar lipid ratios were 0.71 for salmon and 0.39 for krill, with significant differences in lipid class distribution and phospholipid concentrations, determined via acetone fractionation. Polar fractions exhibited high LC-PUFA content: 5.36 % EPA and 13.90 % DHA respectively for salmon, whereas polar lipids in krill contained 12.96 % EPA and 7.26 % DHA. Nutritional and health-related indices including atherogenicity, thrombogenicity, polyene, health-promoting, oxidisability, peroxidisability, and hypo/hypercholesterolemic ratios, highlighted the specific advantages and limitations of each source. The relative proportions of fatty acids influenced the physicochemical properties of the resulting liposomes. Dynamic Light Scattering and Nanoparticle Tracking Analysis revealed particle sizes of ∼86.56 nm for salmon and 176.22 nm for krill liposomes, with highly negative zeta potentials ensuring long-term colloidal stability. Both liposome types showed good size homogeneity, low polydispersity indices (∼0.2), and favorable particle mobility under imaging. Overall, these marine by-products represent sustainable sources of polar lipids naturally enriched in EPA, DHA and astaxanthin, the main carotenoid present with well-documented antioxidant properties, providing functional and nutritional benefits. This highlights their significant potential for the development of lipid-based delivery systems in food, nutraceutical and health applications.","PeriodicalId":93898,"journal":{"name":"Biochimie","volume":" ","pages":""},"PeriodicalIF":3.0,"publicationDate":"2025-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145103188","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Human CXCL17 binds and activates fish GPR25 orthologs. 人类CXCL17结合并激活鱼类GPR25同源物。

IF 3

Biochimie Pub Date : 2025-09-17 DOI: 10.1016/j.biochi.2025.09.009

Wen-Feng Hu, Juan-Juan Wang, Jie Yu, Jun-Jie Yao, Ya-Li Liu, Zeng-Guang Xu, Zhan-Yun Guo

{"title":"Human CXCL17 binds and activates fish GPR25 orthologs.","authors":"Wen-Feng Hu, Juan-Juan Wang, Jie Yu, Jun-Jie Yao, Ya-Li Liu, Zeng-Guang Xu, Zhan-Yun Guo","doi":"10.1016/j.biochi.2025.09.009","DOIUrl":"10.1016/j.biochi.2025.09.009","url":null,"abstract":"C-X-C motif chemokine ligand 17 (CXCL17) functions as a chemoattractant, though its receptor has been controversial. Recent independent studies, including our own, identified CXCL17 as an agonist for the orphan G protein-coupled receptor 25 (GPR25). While GPR25 orthologs are found across fishes to mammals, CXCL17 orthologs appear to be mammalian-specific, leaving the endogenous ligand for non-mammalian GPR25 orthologs unknown. This study unexpectedly found that human CXCL17 exhibits high activity towards GPR25 orthologs from the zebrafish (Danio rerio) and coelacanth (Latimeria chalumnae). Recombinant human CXCL17 efficiently activated both fish GPR25 orthologs in a NanoLuc Binary Technology (NanoBiT)-based β-arrestin recruitment assay, and induced chemotactic movement in transfected human embryonic kidney (HEK) 293T cells expressing fish GPR25. A human CXCL17 mutant lacking three C-terminal residues showed no such effect. A NanoBiT-based binding assay revealed that a SmBiT-tagged human CXCL17 C-terminal fragment specifically bound to secretory large NanoLuc fragment (sLgBiT)-fused fish GPR25 orthologs. Fish GPR25 orthologs had significantly higher cell surface expression in transfected HEK293T cells compared to human GPR25, improving β-arrestin recruitment assay data quality. Despite approximately 400 million years of divergence between humans and fishes, the high activity of human CXCL17 on fish GPR25 orthologs suggests that the CXCL17-GPR25 pair may be conserved across all vertebrates, even though non-mammalian CXCL17 orthologs remain unidentified.","PeriodicalId":93898,"journal":{"name":"Biochimie","volume":" ","pages":""},"PeriodicalIF":3.0,"publicationDate":"2025-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145093078","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

N-3 long-chain polyunsaturated fatty acids in fish physiology: From aquaculture to economic, ecological and public health challenges. N-3长链多不饱和脂肪酸在鱼类生理中的作用：从水产养殖到经济、生态和公共卫生挑战。

IF 3

Biochimie Pub Date : 2025-09-16 DOI: 10.1016/j.biochi.2025.09.007

Mickael Péron, Mathilde Bertrand, Elodie Baranek, Maud Martinat, Philippe Soudant, Marie Vagner, Jérôme Roy

{"title":"N-3 long-chain polyunsaturated fatty acids in fish physiology: From aquaculture to economic, ecological and public health challenges.","authors":"Mickael Péron, Mathilde Bertrand, Elodie Baranek, Maud Martinat, Philippe Soudant, Marie Vagner, Jérôme Roy","doi":"10.1016/j.biochi.2025.09.007","DOIUrl":"10.1016/j.biochi.2025.09.007","url":null,"abstract":"N-3 long-chain polyunsaturated fatty acids (n-3 LC PUFA), particularly eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), are essential molecules to fish physiology, influencing their development, metabolism, immunity, behavior, and reproduction. However, fish have a limited ability to synthesize these fatty acids endogenously, and must obtain them through diet. The dietary availability of these molecules is increasingly challenged by both ecological and aquaculture constraints linked to climate change and to sustainability of ressources (e.g. fisheries). Currently, global changes - including ocean warming - may reduce the availability of these fatty acids in marine food webs, raising concerns for fish population dynamics and aquaculture sustainability that still largely depends on forage fish. In this review, we first summarize the metabolic pathways and tissue distribution of n-3 LC PUFA in freshwater and marine fish, highlighting differences in bioconversion capacities. We then explore the physiological and behavioral consequences of varying dietary n-3 LC PUFA levels in aquaculture feeds and natural environments, including effects on growth, locomotion, cognition, metabolic performance, oxidative status, immune response, and reproductive investment. We also review current alternatives to fish meal and fish oil, such as plant, insect, microbial, and genetically modified sources, and discuss their potential to meet fish nutritional needs. Altogether, this synthesis underscores the current challenge of n-3 LC PUFA dietary shortage for fish health, aquaculture production and nutritional security of human population, and identifies knowledge gaps that must be addressed to ensure both ecological resilience and sustainable aquaculture development in a rapidly changing world.","PeriodicalId":93898,"journal":{"name":"Biochimie","volume":" ","pages":""},"PeriodicalIF":3.0,"publicationDate":"2025-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145088344","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

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