{"title":"Neuromesodermal Progenitor Derived Mesenchymal Stem Cells: A new source for osteogenesis and adipogenesis in vitro.","authors":"Ayşegül Doğan, Selinay Şenkal-Turhan, Ezgi Bulut-Okumuş","doi":"10.1016/j.biochi.2025.06.016","DOIUrl":"https://doi.org/10.1016/j.biochi.2025.06.016","url":null,"abstract":"<p><p>Derivation of adult mesenchymal stem cells (MSCs) from induced pluripotent stem cells (iPSCs) in culture might be an important approach to generate unlimited cell source for clinical therapies. Identification of appropriate progenitor populations during development is of interest in recent years to establish accurate steps in cell differentiation protocols. In the current study, iPSC derived MSCs (iMSC) via neuromesodermal progenitors (NMP) were generated using an established in vitro differentiation protocol. Osteogenic and adipogenic differentiation potential of iMSCs were evaluated by in vitro cell culture assays, gene and protein expression analysis. Protein expression profile of iMSCs and secretome of iMSCs were determined by membrane array and LC-MS/MS analysis. Conditioned medium derived from iMSCs was used in differentiation protocol of preadipocytes and preosteoblast cells. Adipogenic and osteogenic cells were derived from iMSCs in vitro. Protein profile of iMSCs were different compared to dental pulp and adipose stem cells. iMSC secretome has a more prominent role to promote adipogenesis in cultured preadipocytes. In conclusion, MSCs with an adipogenic and osteogenic differentiation capacity can be obtained from iPSC derived NMPs in vitro. Secretome of iMSCs have adiponenic potential on preadipocytes and can be a promising option for future studies.</p>","PeriodicalId":93898,"journal":{"name":"Biochimie","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144531628","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
BiochimiePub Date : 2025-06-25DOI: 10.1016/j.biochi.2025.06.013
Nishanth Kuganesan, Samkeliso Dlamini, Safiyyah Hasan, L M Viranga Tillekeratne, William R Taylor
{"title":"Regulation of Ferroptosis by Transcription Factor E2F1.","authors":"Nishanth Kuganesan, Samkeliso Dlamini, Safiyyah Hasan, L M Viranga Tillekeratne, William R Taylor","doi":"10.1016/j.biochi.2025.06.013","DOIUrl":"10.1016/j.biochi.2025.06.013","url":null,"abstract":"<p><p>The E2F family of transcription factors plays multiple roles in cell cycle regulation. E2F can be inhibited by binding to RB proteins, an interaction that is regulated by CDK phosphorylation of RB. We previously observed that CDKs, RB, and E2F regulate ferroptosis, a type of programmed cell death characterized by catastrophic peroxidation of membrane lipids. Here we investigate the impact of E2F on ferroptosis. E2F1 regulates both pro and anti-ferroptotic proteins including ALOX5, MYC SLC7A11, ATF4, and GPX4 and finally renders a net inhibitory role in ferroptosis. Interestingly, we also obtained evidence for a cell type dependent compensatory effect of E2F3 upon E2F1 depletion. Specifically, downregulation of ferroptotic genes upon E2F1 knockdown fails to occur in an osteosarcoma cell line which upregulates E2F3 under these conditions. Taken together, our study identifies a number of E2F targets with the potential to affect ferroptotic sensitivity.</p>","PeriodicalId":93898,"journal":{"name":"Biochimie","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144512924","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
BiochimiePub Date : 2025-06-25DOI: 10.1016/j.biochi.2025.06.015
Louise Lambert, Amandine Moretton, Géraldine Farge
{"title":"Post-Transcriptional Modifications and Regulation of mRNAs in Human Mitochondria.","authors":"Louise Lambert, Amandine Moretton, Géraldine Farge","doi":"10.1016/j.biochi.2025.06.015","DOIUrl":"https://doi.org/10.1016/j.biochi.2025.06.015","url":null,"abstract":"<p><p>Mitochondria contain their own circular genome (mtDNA), which encodes essential components of the oxidative phosphorylation (OXPHOS) system. Mitochondrial DNA transcription is a unique and relatively simple process, requiring a specialized transcription machinery that consists of a RNA polymerase (POLRMT), two transcription factors (TFAM and TFB2M), and an elongation factor (TEFM). During transcription, a non-canonical initiating nucleotide (NCIN) can be incorporated as the first nucleotide, serving as a 5' cap. Mitochondrial transcription produces large polycistronic transcripts, which are subsequently processed by ribonucleases to generate individual messenger RNAs (mt-mRNAs), ribosomal RNAs (mt-rRNAs), and transfer RNAs (mt-tRNAs). This review will specifically focus on the maturation and regulation of mt-mRNAs. Following their release from the primary transcript, mt-mRNAs undergo various post-transcriptional modifications, including methylation, pseudouridylation, and polyadenylation. These modifications play a crucial role in determining mt-mRNAs fate by influencing their stability, translation efficiency, and overall mitochondrial function. Additionally, the spatial organization of these processes within mitochondrial RNA granules (MRGs) suggests a compartmentalized system for mitochondrial gene regulation, ensuring precise coordination between transcription, processing, and translation. A deeper understanding of these post-transcriptional modifications provides valuable insights into mitochondrial gene expression and its broader impact on cellular metabolism.</p>","PeriodicalId":93898,"journal":{"name":"Biochimie","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144512923","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"5.8S rRNA forms and ribosome heterogeneity in breast cancer.","authors":"Giulia Venturi, Federico Zacchini, Francesca Ruzzi, Angelo Gianluca Corradini, Margherita Serra, Marianna Penzo, Davide Treré, Pier-Luigi Lollini, Lorenzo Montanaro","doi":"10.1016/j.biochi.2025.05.010","DOIUrl":"10.1016/j.biochi.2025.05.010","url":null,"abstract":"<p><p>Ribosome heterogeneity can contribute to translation regulation in terms of mRNA selection and translation efficiency. This is particularly true for cancer cells in which oncoribosomes are reported to translate mRNAs encoding for proteins involved in cancer progression. Among other factors, a source of ribosome heterogeneity not yet characterized could be represented by 5.8S rRNA and its three forms distinguished based on their 5' sequence. So far, little is known about the role of the presence of these isoforms in mature ribosomes and how they may contribute to human pathology. Here we investigated the relative abundance of the three 5.8S rRNA isoforms in different contexts. Analyzing total and polysomal RNA from cancer cell lines we detected all the three forms recruited in actively translating ribosomes consistently to the basal levels of their expression. Moreover, we showed that changes in the relative abundance of 5.8S rRNA isoforms can be linked to the process of tumorigenesis in a human HER2 transgenic mouse model which develops spontaneous mammary carcinomas. Finally, from the analysis of breast cancer samples, we observed significant correlations between tumor grade, estrogen receptor status and patient prognosis with the relative abundance of 5.8S rRNA isoforms. These results suggest an additional level of complexity involving 5.8S rRNA and ribosome heterogeneity in cancer pathology.</p>","PeriodicalId":93898,"journal":{"name":"Biochimie","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144188674","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
BiochimiePub Date : 2025-05-16DOI: 10.1016/j.biochi.2025.05.005
Fiz da Costa, Margaux Mathieu-Resuge, Fabienne Le Grand, Claudie Quéré, Gabriel V Markov, Gary H Wikfors, Philippe Soudant
{"title":"Sterol biosynthesis and phytosterol bioconversion in Crassostrea gigas larvae: new evidence from mass-balance feeding studies.","authors":"Fiz da Costa, Margaux Mathieu-Resuge, Fabienne Le Grand, Claudie Quéré, Gabriel V Markov, Gary H Wikfors, Philippe Soudant","doi":"10.1016/j.biochi.2025.05.005","DOIUrl":"10.1016/j.biochi.2025.05.005","url":null,"abstract":"<p><p>Dietary sterols are important for bivalve larval growth and survival. The aim of the study was to determine quantitatively sterol incorporation and synthesis in Pacific oyster Crassostrea gigas larvae by means of a mass-balance approach. The flow-through larval rearing technique allowed accurate quantification of microalgal ingestion and consequently of sterol ingestion. Sterol incorporation was calculated using the sterol composition of the larvae between 2 sample points. Two sets of experiments were done using two cultured microalgae: Tisochrysis lutea (T) and Chaetoceros neogracile (Cg) as mono- and bi-specific diets (TCg). Accumulation of tissue sterols in oyster larvae, in addition to those present in the diet, indicate that C. gigas larvae appear to have the ability to synthetize sterols de novo under low dietary sterol supply, e.g., when fed the T diet. Sterol synthesis was dependent upon sterol dietary supply; larvae fed T exhibited greater sterol incorporation at the pediveliger stage than larvae fed TCg. Larval sterol compositions under the different dietary regimes indicate likely bioconversion pathways modifying dietary sterols. Larvae fed T bioconverted dietary brassicasterol mainly to cholesterol via a 22-dehydrocholesterol intermediate. Brassicasterol was also actively synthetized in larvae fed T and TCg, suggesting a possible metabolic role of this sterol in C. gigas larvae. Apparent desmosterol synthesis under all experimental conditions suggests a role as a membrane component or as an intermediate in cholesterol synthesis. Our data also indicate that C. gigas larvae require approximately 13 ng cholesterol larvae<sup>-1</sup> to achieve competence for metamorphosis. This mass-balance approach will allow the determination of other biochemical requirements in larval nutrition.</p>","PeriodicalId":93898,"journal":{"name":"Biochimie","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-05-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144096198","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
BiochimiePub Date : 2025-05-12DOI: 10.1016/j.biochi.2025.05.003
Oriana Puidebat, Sylvain Egloff
{"title":"The 7SK snRNP complex: a critical regulator in carcinogenesis.","authors":"Oriana Puidebat, Sylvain Egloff","doi":"10.1016/j.biochi.2025.05.003","DOIUrl":"10.1016/j.biochi.2025.05.003","url":null,"abstract":"<p><p>Cyclin-Dependent Kinase 9 (CDK9) is a critical regulator of transcriptional elongation, functioning within the Positive Transcription Elongation Factor b (P-TEFb) complex alongside Cyclin T1. P-TEFb facilitates the release of RNA polymerase II (RNAPII) from promoter-proximal pausing, thereby enabling productive transcriptional elongation. CDK9 activity is tightly controlled by the 7SK small nuclear ribonucleoprotein (7SK snRNP) complex, comprising 7SK snRNA, LARP7, MEPCE, and HEXIM1/2. Under homeostatic conditions, the 7SK snRNP sequesters and inactivates a fraction of P-TEFb, maintaining it in a repressed state. However, in response to cellular stress or increased transcriptional demand, P-TEFb is released from 7SK snRNP, activating CDK9 to ensure precise, context-dependent transcriptional control. This regulatory switch allows dynamic adaptation to environmental and intracellular cues. Emerging evidence implicates 7SK snRNP deregulation in cancer progression. This review explores the intricate interplay between 7SK snRNP and CDK9, highlighting how disruptions in individual 7SK snRNP components drive transcriptional imbalances, amplify oncogenic programs, and promote a tumorigenic environment.</p>","PeriodicalId":93898,"journal":{"name":"Biochimie","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-05-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144082693","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"The complex post-transcriptional regulation of genes coding for methionine adenosyl transferase: New insights for liver cancer.","authors":"Amina Doudou Tellai, Vincent Haghnejad, Justine Antoine, Basma Khemiri Merouani, Jean-Pierre Bronowicki, Natacha Dreumont","doi":"10.1016/j.biochi.2025.05.001","DOIUrl":"10.1016/j.biochi.2025.05.001","url":null,"abstract":"<p><p>Methionine adenosyltransferases (MATs) catalyze the synthesis of S-adenosylmethionine (SAM), the universal methyl donor involved in methylation reactions, redox balance, and polyamine synthesis. In mammals, three MAT genes, MAT1A, MAT2A, and MAT2B, exhibit tissue-specific expression, with MAT1A predominating in healthy liver and MAT2A/MAT2B upregulated during liver injury and malignancy. A shift from MAT1A to MAT2A/MAT2B expression is a hallmark of hepatocellular carcinoma (HCC), contributing to decreased SAM levels and promoting tumorigenesis. Recent findings highlight the pivotal role of post-transcriptional regulation in controlling MAT gene expression. N6-methyladenosine (m6A) modification, the most prevalent internal mRNA modification, plays a dynamic role in determining the fate of MAT2A mRNA. m6A marks regulate MAT2A mRNA splicing and stability in response to stress and metabolic changes. Additionally, RNA-binding proteins (RBPs) such as ELAVL1 and hnRNPD bind to MAT mRNAs, modulating their stability and translation. Dysregulation of these RBPs in liver disease alters MAT expression profiles. Non-coding RNAs, including microRNAs such as miR-29, miR-21, and miR-485, and long non-coding RNAs such as LINC00662 and SNGH6, modulate MAT expression post-transcriptionally by targeting MAT transcripts directly or influencing RNA-binding proteins (RBPs) and m6A writers/readers. Together, these mechanisms form a complex and intricate post-transcriptional regulatory network that governs MAT activity in physiological and pathological states. This review examines emerging insights into MAT post-transcriptional regulation, focusing on its implications for liver cancer, and opens new avenues for developing therapies that target these regulatory mechanisms.</p>","PeriodicalId":93898,"journal":{"name":"Biochimie","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144060767","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Master of disguise: ribosomal protein L5 beyond translation.","authors":"Guglielmo Rambaldelli, Lorenza Bacci, Daniela Pollutri, Kamil Filipek, Marianna Penzo","doi":"10.1016/j.biochi.2025.03.009","DOIUrl":"10.1016/j.biochi.2025.03.009","url":null,"abstract":"<p><p>Ribosomal proteins (RPs), key components of ribosomes, are traditionally associated with protein synthesis. However, emerging evidence suggests their involvement in diverse cellular functions beyond ribosomal biogenesis and translation, including transcriptional regulation. This study aimed at investigating the potential of RPs as transcriptional regulators by analyzing their interacting protein network. A subset of RP interactors exhibiting transcriptional regulatory functions was subjected to Gene Ontology analysis to identify enriched functional pathways. The results indicated that these interactions may play a role in different cellular pathways relevant to a number of biological processes, including cancer. To further explore this hypothesis, a virtual knockdown of RPL5 was performed in ovarian and breast cancer public data. As proof of concept the same experiments were conducted in vitro to validate the computational findings, confirming the potential of RPL5 in transcriptional regulation in cancer. This seminal study provides a foundation for future investigations into the multifaceted roles of RPs in the regulation of gene expression in physiological and pathological contexts.</p>","PeriodicalId":93898,"journal":{"name":"Biochimie","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143789395","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
BiochimiePub Date : 2025-02-20DOI: 10.1016/j.biochi.2025.02.002
Jean-Philippe Robin, Vincent Mocquet
{"title":"Hijacking a real time detection thermocycler for enzymology: Improvement of a fluorescent bulk assay monitoring helicase activity.","authors":"Jean-Philippe Robin, Vincent Mocquet","doi":"10.1016/j.biochi.2025.02.002","DOIUrl":"10.1016/j.biochi.2025.02.002","url":null,"abstract":"<p><p>Helicases are enzymes involved in all aspects of nucleic acid synthesis, regulation and degradation. As a consequence, several methods were developed to monitor their enzymatic activity. In this report, we described an improvement of bulk fluorescent helicase assays to overcome their specific limitations (cost, health and safety regulations, etc.). Using a real time detection thermocycler to monitor the fluorescence in real-time, we managed to precisely control the initiation of the helicase reaction through temperature tuning. Therefore, we were able to demonstrate that this setup could provide a qualitative and a quantitative evaluation of the helicase domain of the UPF1 helicase (UPF1-HD) and that several fluorophores could be used in parallel during the same run.</p>","PeriodicalId":93898,"journal":{"name":"Biochimie","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143476917","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
BiochimiePub Date : 2024-05-15DOI: 10.1016/j.biochi.2024.05.014
Ivan Petushkov, Andrey Feklistov, Andrey Kulbachinskiy
{"title":"Highly specific aptamer trap for extremophilic RNA polymerases.","authors":"Ivan Petushkov, Andrey Feklistov, Andrey Kulbachinskiy","doi":"10.1016/j.biochi.2024.05.014","DOIUrl":"https://doi.org/10.1016/j.biochi.2024.05.014","url":null,"abstract":"<p><p>During transcription initiation, the holoenzyme of bacterial RNA polymerase (RNAP) specifically recognizes promoters using a dedicated σ factor. During transcription elongation, the core enzyme of RNAP interacts with nucleic acids mainly nonspecifically, by stably locking the DNA template and RNA transcript inside the main cleft. Here, we present a synthetic DNA aptamer that is specifically recognized by both core and holoenzyme RNAPs from extremophilic bacteria of the Deinococcus-Thermus lineage. The aptamer binds RNAP with subnanomolar affinities, forming extremely stable complexes even at high ionic strength conditions, blocks RNAP interactions with the DNA template and inhibits RNAP activity during transcription elongation. We propose that the aptamer binds at a conserved site within the downstream DNA-binding cleft of RNAP and traps it in an inactive conformation. The aptamer can potentially be used for structural studies to reveal RNAP conformational states, affinity binding of RNAP and associated factors, and screening of transcriptional inhibitors.</p>","PeriodicalId":93898,"journal":{"name":"Biochimie","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140960867","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}