Biochimie最新文献_第2页

Human CXCL17 binds and activates fish GPR25 orthologs. 人类CXCL17结合并激活鱼类GPR25同源物。

IF 3

Biochimie Pub Date : 2025-09-17 DOI: 10.1016/j.biochi.2025.09.009

Wen-Feng Hu, Juan-Juan Wang, Jie Yu, Jun-Jie Yao, Ya-Li Liu, Zeng-Guang Xu, Zhan-Yun Guo

{"title":"Human CXCL17 binds and activates fish GPR25 orthologs.","authors":"Wen-Feng Hu, Juan-Juan Wang, Jie Yu, Jun-Jie Yao, Ya-Li Liu, Zeng-Guang Xu, Zhan-Yun Guo","doi":"10.1016/j.biochi.2025.09.009","DOIUrl":"10.1016/j.biochi.2025.09.009","url":null,"abstract":"C-X-C motif chemokine ligand 17 (CXCL17) functions as a chemoattractant, though its receptor has been controversial. Recent independent studies, including our own, identified CXCL17 as an agonist for the orphan G protein-coupled receptor 25 (GPR25). While GPR25 orthologs are found across fishes to mammals, CXCL17 orthologs appear to be mammalian-specific, leaving the endogenous ligand for non-mammalian GPR25 orthologs unknown. This study unexpectedly found that human CXCL17 exhibits high activity towards GPR25 orthologs from the zebrafish (Danio rerio) and coelacanth (Latimeria chalumnae). Recombinant human CXCL17 efficiently activated both fish GPR25 orthologs in a NanoLuc Binary Technology (NanoBiT)-based β-arrestin recruitment assay, and induced chemotactic movement in transfected human embryonic kidney (HEK) 293T cells expressing fish GPR25. A human CXCL17 mutant lacking three C-terminal residues showed no such effect. A NanoBiT-based binding assay revealed that a SmBiT-tagged human CXCL17 C-terminal fragment specifically bound to secretory large NanoLuc fragment (sLgBiT)-fused fish GPR25 orthologs. Fish GPR25 orthologs had significantly higher cell surface expression in transfected HEK293T cells compared to human GPR25, improving β-arrestin recruitment assay data quality. Despite approximately 400 million years of divergence between humans and fishes, the high activity of human CXCL17 on fish GPR25 orthologs suggests that the CXCL17-GPR25 pair may be conserved across all vertebrates, even though non-mammalian CXCL17 orthologs remain unidentified.","PeriodicalId":93898,"journal":{"name":"Biochimie","volume":" ","pages":""},"PeriodicalIF":3.0,"publicationDate":"2025-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145093078","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

N-3 long-chain polyunsaturated fatty acids in fish physiology: From aquaculture to economic, ecological and public health challenges. N-3长链多不饱和脂肪酸在鱼类生理中的作用：从水产养殖到经济、生态和公共卫生挑战。

IF 3

Biochimie Pub Date : 2025-09-16 DOI: 10.1016/j.biochi.2025.09.007

Mickael Péron, Mathilde Bertrand, Elodie Baranek, Maud Martinat, Philippe Soudant, Marie Vagner, Jérôme Roy

{"title":"N-3 long-chain polyunsaturated fatty acids in fish physiology: From aquaculture to economic, ecological and public health challenges.","authors":"Mickael Péron, Mathilde Bertrand, Elodie Baranek, Maud Martinat, Philippe Soudant, Marie Vagner, Jérôme Roy","doi":"10.1016/j.biochi.2025.09.007","DOIUrl":"10.1016/j.biochi.2025.09.007","url":null,"abstract":"N-3 long-chain polyunsaturated fatty acids (n-3 LC PUFA), particularly eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), are essential molecules to fish physiology, influencing their development, metabolism, immunity, behavior, and reproduction. However, fish have a limited ability to synthesize these fatty acids endogenously, and must obtain them through diet. The dietary availability of these molecules is increasingly challenged by both ecological and aquaculture constraints linked to climate change and to sustainability of ressources (e.g. fisheries). Currently, global changes - including ocean warming - may reduce the availability of these fatty acids in marine food webs, raising concerns for fish population dynamics and aquaculture sustainability that still largely depends on forage fish. In this review, we first summarize the metabolic pathways and tissue distribution of n-3 LC PUFA in freshwater and marine fish, highlighting differences in bioconversion capacities. We then explore the physiological and behavioral consequences of varying dietary n-3 LC PUFA levels in aquaculture feeds and natural environments, including effects on growth, locomotion, cognition, metabolic performance, oxidative status, immune response, and reproductive investment. We also review current alternatives to fish meal and fish oil, such as plant, insect, microbial, and genetically modified sources, and discuss their potential to meet fish nutritional needs. Altogether, this synthesis underscores the current challenge of n-3 LC PUFA dietary shortage for fish health, aquaculture production and nutritional security of human population, and identifies knowledge gaps that must be addressed to ensure both ecological resilience and sustainable aquaculture development in a rapidly changing world.","PeriodicalId":93898,"journal":{"name":"Biochimie","volume":" ","pages":""},"PeriodicalIF":3.0,"publicationDate":"2025-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145088344","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Application of transposon expression system in recombinant protein expression and gene therapy. 转座子表达系统在重组蛋白表达及基因治疗中的应用。

IF 3

Biochimie Pub Date : 2025-09-16 DOI: 10.1016/j.biochi.2025.09.008

Zimeng Han, Xuelian Han, Wanting Cheng, Shaolei Geng, Weidong Li, Tianyun Wang, Xiaoyin Wang

引用次数: 0

Exploring glycobiomarkers beyond senescence-associated β-galactosidase for studying aging in vitro. 探索除衰老相关β-半乳糖苷酶外的糖生物标志物用于体外衰老研究。

IF 3

Biochimie Pub Date : 2025-09-11 DOI: 10.1016/j.biochi.2025.09.005

Lucia Račková, Rebeka Kodríková, Marek Nemčovič, Erika Csekes, Zuzana Pakanová

{"title":"Exploring glycobiomarkers beyond senescence-associated β-galactosidase for studying aging in vitro.","authors":"Lucia Račková, Rebeka Kodríková, Marek Nemčovič, Erika Csekes, Zuzana Pakanová","doi":"10.1016/j.biochi.2025.09.005","DOIUrl":"10.1016/j.biochi.2025.09.005","url":null,"abstract":"Cellular senescence, a phenomenon believed to contribute significantly to aging and age-related diseases, can be readily achieved under cell culture conditions. Alterations in the N-glycosylation of human blood glycoproteins have emerged as promising biomarkers of aging. However, knowledge about the in vitro use of glycobiomarkers for assessing cellular senescence remains limited. Our study utilized MALDI-TOF mass spectrometry to compare the alterations in the N-glycome of replicatively senescent human dermal fibroblasts and glyoxal- and H2O2-executed SIPS cells. In this regard, fibroblasts at both early and late stages of H2O2-induced SIPS were evaluated. In all models, the onset of senescence was marked by an increase in levels of paucimannose Hex2-3GlcNAc2Fuc1 species along with reductions in fucosylated di-galactosylated biantennary N-glycans (Hex5HexNAc4Fuc1-2), which coincided with an upregulation of β-galactosidase (β-gal) activities. A nearly 40 % and 30 % decline in complex sialylated N-glycans, and around a 60 % and 40 % reduction in free oligosaccharides were also observed in the terminal passage of RS fibroblasts and late-stage H2O2-induced SIPS, respectively. Our findings suggest that the onset of RS and SIPS generally influenced the same types of N-glycans. However, the extent of influences varied and was more or less proportional to SA-β-gal expression levels. In contrast to standard markers of senescence, such as SA-β-gal, the MALDI-TOF MS glycomics analysis also allows for discrimination between the early onset and the late stages of senescence.","PeriodicalId":93898,"journal":{"name":"Biochimie","volume":" ","pages":""},"PeriodicalIF":3.0,"publicationDate":"2025-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145058772","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Decoding G-quadruplex stability: The role of loop architecture and sequence context in the human genome. 解码g -四重体稳定性：环结构和序列背景在人类基因组中的作用。

IF 3

Biochimie Pub Date : 2025-09-11 DOI: 10.1016/j.biochi.2025.09.006

Jiye Fu, Tianyu Chen, Na Lu, Xuan Pan, Jing Tu

{"title":"Decoding G-quadruplex stability: The role of loop architecture and sequence context in the human genome.","authors":"Jiye Fu, Tianyu Chen, Na Lu, Xuan Pan, Jing Tu","doi":"10.1016/j.biochi.2025.09.006","DOIUrl":"10.1016/j.biochi.2025.09.006","url":null,"abstract":"Guanine-rich sequences are widely distributed throughout the human genome and are capable of forming intramolecular G-quadruplex (G4) structures through Hoogsteen hydrogen bonding. These structures have been implicated in diverse regulatory processes. While extensive studies have established that loop architecture-particularly loop length and composition-profoundly affects G4 structural stability, most investigations have relied on synthetic sequences with predefined loop configurations that do not accurately reflect genomic contexts. In the current study, we analyzed the chain composition and stability of G-quadruplexes within the human genome to clarify the relationship between them by high throughput sequencing data. We utilized G4-forming sequences identified by G4-seq and G4-miner-two sequencing-based methods that detect G4s through polymerase stalling-associated drops in sequencing quality scores, where more stable structures produce stronger signals and thus higher detection rates-as the primary dataset. Our analysis revealed a negative correlation between total loop length and G4 stability, whereas individual loop length distributions exhibited minimal influence. Interestingly, G4s with short loops frequently occur in the genome as microsatellites or tandem atypical G4 arrays, resulting in structural stability profiles that deviate from those observed in synthetic G4 motifs in vitro. Molecular dynamics simulations incorporating native flanking sequences further corroborated these findings, underscoring the importance of genomic context in determining G4 stability. We note that the research was restricted to canonical G4s, which may limit the generality of our conclusions.","PeriodicalId":93898,"journal":{"name":"Biochimie","volume":" ","pages":""},"PeriodicalIF":3.0,"publicationDate":"2025-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145058804","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Molecular-level lipid studies on marine microphytobenthic biofilms: a mini-review. 海洋微底栖植物生物膜的分子水平脂质研究综述。

IF 3

Biochimie Pub Date : 2025-09-09 DOI: 10.1016/j.biochi.2025.09.004

Sara Finnerty, Jose Antonio Galán-Pérez, Yunhai Li, Brian Kelleher, Shane O'Reilly

{"title":"Molecular-level lipid studies on marine microphytobenthic biofilms: a mini-review.","authors":"Sara Finnerty, Jose Antonio Galán-Pérez, Yunhai Li, Brian Kelleher, Shane O'Reilly","doi":"10.1016/j.biochi.2025.09.004","DOIUrl":"10.1016/j.biochi.2025.09.004","url":null,"abstract":"Microphytobenthic biofilms in tidal flats are globally extensive microbial ecosystems that play crucial roles in supporting benthic and seabird diversity and in coastal carbon cycling. Despite their importance and global extent, few studies have measured the molecular composition and biotechnological potential of these biofilms. A systematic and bibliometric approach was performed to curate and analyse peer-reviewed original research articles from this perspective. Network visualization highlighted the core focus for molecular-level studies on microphytobenthic biofilms was gas chromatography-mass spectrometry (GC-MS) analysis of major fatty acids and their use as tracers for ecological, diet and biogeochemical purposes. 9Z-hexadecenoic acid (C16:1n-7), 5Z,8Z,11Z,14Z, 17Z-eicosapentaenoic acid (C20:5n-3)) and tetradecanoic acid (C14:0) were the major fatty acid species common to MPB across all studies. However, few studies to date have used a comprehensive untargeted mass spectrometric approach and none have combined these approaches with assessing the functional role of lipids and their biotechnological application by bioactivity screening. The review highlights the need such work including more representative studies globally. The sensitivity of these biofilms to anthropogenic pollution highlights the urgency of research on this topic and that biodiscovery should be underpinned by environmental protection and circular economy principles.","PeriodicalId":93898,"journal":{"name":"Biochimie","volume":" ","pages":""},"PeriodicalIF":3.0,"publicationDate":"2025-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145042704","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

NFATc3 and PML synergistically regulate tumor-associated gene expression in a SUMOylation-Independent manner. NFATc3和PML以不依赖于sumoyla的方式协同调节肿瘤相关基因表达。

IF 3

Biochimie Pub Date : 2025-09-08 DOI: 10.1016/j.biochi.2025.09.002

Ting Kang, Ruizhe Huang, Ruiheng Wang, Han Liu, Siyu Chen

{"title":"NFATc3 and PML synergistically regulate tumor-associated gene expression in a SUMOylation-Independent manner.","authors":"Ting Kang, Ruizhe Huang, Ruiheng Wang, Han Liu, Siyu Chen","doi":"10.1016/j.biochi.2025.09.002","DOIUrl":"10.1016/j.biochi.2025.09.002","url":null,"abstract":"The nuclear factor of activated T cells 3 (NFATc3) plays a significant role in various cancer-related processes, but its interactions with transcriptional modulators, particularly Promyelocytic Leukemia protein (PML), remain poorly understood. PML, a nuclear scaffold protein, is involved in tumor suppression and transcriptional regulation. This study investigates the interaction between NFATc3 and PML, focusing on the role of SUMOylation and its impact on downstream target genes. In vitro experiments, including mass spectrometry and Co-immunoprecipitation (Co-IP), were conducted to explore this interaction. Additionally, constructs with lysine-to-arginine (K→R) mutations at key SUMOylation sites were generated to determine whether PML SUMOylation is necessary for its interaction with NFATc3. We also assessed the impact of NFATc3 SUMOylation on its binding to PML. Chromatin immunoprecipitation (ChIP) and quantitative real-time PCR (qRT-PCR) were employed to measure the expression of downstream genes (Lgr5 and Olfm4) under NFATc3 and PML overexpression or knockdown conditions. Pharmacological treatment with arsenic sulfide (As4S4) was used to further investigate modulation of the PML-NFATc3 axis. Our findings revealed that the NFATc3-PML interaction is independent of the SUMOylation status of PML. Additionally, mutations in NFATc3 SUMOylation sites did not affect its binding to PML. The PML-NFATc3 axis regulates Lgr5 and Olfm4 expression, and co-expression of NFATc3 and PML synergistically upregulated these genes. Arsenic sulfide treatment reduced this synergistic effect, indicating its potential as a modulator. This study provides new insights into the regulatory mechanisms of NFATc3 and PML, suggesting potential therapeutic targets in cancer.","PeriodicalId":93898,"journal":{"name":"Biochimie","volume":" ","pages":""},"PeriodicalIF":3.0,"publicationDate":"2025-09-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145034798","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Novel alternative transcripts of TLR8 and TLR9 reveal evolutionary pressure to conserve protein structure. TLR8和TLR9的新转录本揭示了保护蛋白质结构的进化压力。

IF 3

Biochimie Pub Date : 2025-09-05 DOI: 10.1016/j.biochi.2025.09.003

Jorge Martinez-Laso, Isabel Cervera, María José Muñoz-Gómez, Clara Sánchez-Menéndez, Ana Salamanca-Soto, Montserrat Torres, Mayte Coiras

{"title":"Novel alternative transcripts of TLR8 and TLR9 reveal evolutionary pressure to conserve protein structure.","authors":"Jorge Martinez-Laso, Isabel Cervera, María José Muñoz-Gómez, Clara Sánchez-Menéndez, Ana Salamanca-Soto, Montserrat Torres, Mayte Coiras","doi":"10.1016/j.biochi.2025.09.003","DOIUrl":"10.1016/j.biochi.2025.09.003","url":null,"abstract":"TLR8 and TLR9 are innate immune receptors belonging to the TLR family that are essential for viral recognition and early immune activation. Their dysfunction is linked to increased susceptibility to infections. TLR8 detects viral single- and double-stranded RNA, while TLR9 recognizes viral DNA molecules with CpG motifs. Six TLR8 alternative transcripts (V1, V2, V5, V6, V7, and V8) and nine TLR9 (V1, A, B, C, D, E, V8, V9, and V10) have been previously described in humans. In the present study, we have performed a comprehensive analysis of TLR8 and TLR9 transcripts in a healthy population and two new TLR8 transcripts (V3 and V4) and four new TLR9 transcripts (V2, V5, V6, and V7) were found. The main mechanisms for the generation of different mRNA variants were the insertion of non-coding regions and the loss of whole or partial exons. These changes result in the loss or insertion of new amino acids but only modify the initial leucine-rich repeat (LRR) region and preserve the rest of the receptor's complete structure. From the results obtained, we can deduce that there seems to be a strong evolutionary drive to maintain TLR8 and TLR9 functionality, unlike other innate immune response receptors.","PeriodicalId":93898,"journal":{"name":"Biochimie","volume":" ","pages":""},"PeriodicalIF":3.0,"publicationDate":"2025-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145016936","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The unconventional RNA-binding activity of metabolic enzymes. 代谢酶的非常规rna结合活性。

IF 3

Biochimie Pub Date : 2025-09-03 DOI: 10.1016/j.biochi.2025.08.017

Ana Luisa Dian, Stéphan Vagner

{"title":"The unconventional RNA-binding activity of metabolic enzymes.","authors":"Ana Luisa Dian, Stéphan Vagner","doi":"10.1016/j.biochi.2025.08.017","DOIUrl":"10.1016/j.biochi.2025.08.017","url":null,"abstract":"Metabolism involves a wide range of pathways and chemical reactions catalysed by specialized enzymes whose activity is fundamental for living cells. In the past three decades, metabolic enzymes have emerged as critical regulators of gene expression, thus revealing unexpected functions beyond their canonical metabolic roles. In this Review, we discuss the evidences that these enzymes, with a particular focus on enzymes participating in the glucose metabolism, can directly bind RNA. This binding has been recurrently shown to be involved in the post-trasncriptional gene regulation, by influencing processes such as RNA stability, localization, translation, and degradation. Although the mechanisms underlying RNA-enzyme interactions and their regulation are still not fully elucidated, several reports suggest that some of these interactions can be influenced by substrates, metabolites, and cellular metabolic states. In contrast, direct and specific binding of RNAs was also shown to regulate the activity, stability, interaction and localization of the enzymes. The discovery of the non-canonical RNA-binding activity of metabolic enzymes not only expands our understanding of these seemingly well-characterized proteins, but also provides new perspectives on the integration of metabolic and gene regulatory networks, besides revealing potential therapeutic vulnerabilities.","PeriodicalId":93898,"journal":{"name":"Biochimie","volume":" ","pages":""},"PeriodicalIF":3.0,"publicationDate":"2025-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145006980","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Functional characterization of a plastidial cytochrome b5-fused Δ4-desaturase from Ostreococcus tauri in higher plants. 高等植物中金黄色葡萄球菌质体细胞色素b5融合Δ4-desaturase的功能表征。

IF 3

Biochimie Pub Date : 2025-09-02 DOI: 10.1016/j.biochi.2025.08.019

M Miklaszewska, R E Gomez, P Van Delft, M Le Guédard, C Chambaud, C Mirande-Bret, L Fouillen, F Corellou, F Domergue

{"title":"Functional characterization of a plastidial cytochrome b5-fused Δ4-desaturase from Ostreococcus tauri in higher plants.","authors":"M Miklaszewska, R E Gomez, P Van Delft, M Le Guédard, C Chambaud, C Mirande-Bret, L Fouillen, F Corellou, F Domergue","doi":"10.1016/j.biochi.2025.08.019","DOIUrl":"10.1016/j.biochi.2025.08.019","url":null,"abstract":"Marine microalgae are the primary producers of important lipids in oceanic ecosystems. In particular, they sustain the food web with omega-3 very-long-chain polyunsaturated fatty acids (n-3 PUFAs), which play a protective role against various human metabolic disorders and are thus considered highly beneficial to health. Ostreococcus tauri is a marine pico-eukaryote that contains high levels of several n-3 PUFAs, including docosahexaenoic acid (22:6n3; DHA), octadecapentaenoic acid (18:5n3, OPA), and hexadecatetraenoic acid (16:4n3), each with a distinct distribution. While DHA and OPA are restricted to microsomal and plastidial lipids, respectively, 16:4n3 is found in galactolipids as well as in betaine and neutral lipids. The genome of O. tauri contains 14 genes encoding fatty acid desaturases. In this study, we characterized the enzyme encoded by OT_ostta13g01550 (Ot13bDES) as a plastidial cytochrome b5-fused delta-4 desaturase involved in 16:4n3 biosynthesis. Transient heterologous expression of Ot13bDES in Nicotiana benthamiana led to the production of 16:4n3 and 16:3n6, but failed to produce 18:5n3 when Ot13bDES was coexpressed with plastidial Δ6-desaturases, suggesting Ot13bDES has a strict Δ4 regioselectivity. Lipidomic analyses of stable transgenic Arabidopsis lines further showed a nearly 100 % conversion rate of 16:3n3 to 16:4n3 in the best-performing lines, demonstrating that Ot13bDES has a very high catalytic activity. Additionally, 16:4n3 was predominantly localized to monogalactosyldiacylglycerol (MGDG). This study provides the first functional characterization of a plastidial cytochrome b5-fused delta-4 desaturase through heterologous expression in higher plants.","PeriodicalId":93898,"journal":{"name":"Biochimie","volume":" ","pages":""},"PeriodicalIF":3.0,"publicationDate":"2025-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145002130","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0