Archives of oral biology最新文献

{"title":"Are hypoxia-related proteins associated with the invasiveness of glandular odontogenic cysts? A multicenter study.","authors":"Rafaela de Albuquerque Dias, Karolyny Martins Balbinot, Karine Duarte da Silva, Ana Paula Neutzling Gomes, Carla Mosconi, Elismauro Franscisco de Mendonça, Sandra Beatriz Chaves Tarquinio, Sérgio de Melo Alves Junior, Maria Cássia Ferreira de Aguiar, João de Jesus Viana Pinheiro","doi":"10.1016/j.archoralbio.2024.106151","DOIUrl":"https://doi.org/10.1016/j.archoralbio.2024.106151","url":null,"abstract":"<p><strong>Objective: </strong>The study aimed to investigate the expression of hypoxia markers associated with invadopodia in glandular odontogenic cysts and to explore an association between this expression with the aggressive biological behaviour of this odontogenic cyst.</p><p><strong>Design: </strong>Immunohistochemistry was employed to assess the expression of hypoxia-inducible factor 1 alpha (HIF-1α), notch homologous protein of the neurogenic locus 1 (NOTCH-1), disintegrin and metalloproteinase-12 (ADAM-12), and heparin-binding epidermal growth factor (HB-EGF) in 17 samples of glandular odontogenic cysts, 10 samples of calcifying odontogenic cysts, and 10 samples of dental follicles.</p><p><strong>Results: </strong>The glandular odontogenic cyst samples exhibited increased expression of HIF-1α, NOTCH-1, ADAM-12 and HBEGF proteins compared with calcifying odontogenic cyst and dental follicle samples. HIF-1α demonstrated localization primarily within the nuclei of cystic epithelial cells of the glandular odontogenic cyst. NOTCH-1 and ADAM-12 exhibited expression in the cytoplasm and nuclei of epithelial and mucous cells of the glandular odontogenic cyst, of whereas HB-EGF was predominantly expressed in the cytoplasm. Weak labeling of these proteins was observed in the odontogenic epithelium of the calcifying odontogenic cyst and dental follicle samples.</p><p><strong>Conclusions: </strong>The hypoxia-related signaling proteins are overexpressed in glandular odontogenic cyst when compared with calcifying odontogenic cyst and dental follicle. The reported aggressiveness of glandular odontogenic cyst can be partially explained by the expression of these proteins.</p>","PeriodicalId":93882,"journal":{"name":"Archives of oral biology","volume":"171 ","pages":"106151"},"PeriodicalIF":0.0,"publicationDate":"2024-11-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142792938","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The activation level of the floor-of-the-mouth muscles is systematically modulated using tongue-pressing and swallowing tasks in healthy elderly.","authors":"Jong-Chi Oh","doi":"10.1016/j.archoralbio.2024.106155","DOIUrl":"https://doi.org/10.1016/j.archoralbio.2024.106155","url":null,"abstract":"<p><strong>Objective: </strong>This study aimed to examine how muscle activity of the floor-of-the-mouth (FOM) muscles changes with different target exercise intensities of 40 %, 60 %, 80 %, and 100 % of maximum isometric pressure (MIP) during tongue-pressing and swallowing tasks in healthy elderly.</p><p><strong>Design: </strong>This prospective, repeated-measures within-participant study included 35 participants (mean age: 75.2 ± 4.8 years, 26 women). Each participant performed 16 tasks using the Iowa Oral Performance Instrument (IOPI) in a randomized order, with two repetitions per task (anterior/posterior tongue × pressing/swallowing task × 40/60/80/100 % of the MIP). Furthermore, FOM-muscle activity during the task was simultaneously measured using surface electromyography. Statistical analysis was conducted using repeated-measures analysis of variance (ANOVA).</p><p><strong>Results: </strong>Significant differences in FOM-muscle activity were observed across most intensity levels during pressing tasks (p < 0.001), with fewer significant differences noted during swallowing tasks, particularly involving the posterior tongue. A significant correlation was found between tongue pressure of the anterior/posterior tongue and FOM-muscle activation level (p < 0.001 and r = 0.517 and 0.447 for the isometric tongue pressure of the anterior and posterior tongue, respectively; r = 0.370 for the swallowing pressure of the anterior tongue) except for posterior tongue swallowing task.</p><p><strong>Conclusions: </strong>These findings suggest that healthy elderly can systematically modulate suprahyoid muscle activity using a tongue-pressure-measurement device when performing accuracy training. However, they may require personalized systematic feedback and sufficient practice. The study underscores the potential of the IOPI in swallowing rehabilitation for elderly, while also highlighting the need for further research on patients with dysphagia.</p>","PeriodicalId":93882,"journal":{"name":"Archives of oral biology","volume":"171 ","pages":"106155"},"PeriodicalIF":0.0,"publicationDate":"2024-11-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142795875","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multi-strain probiotic formula modulates expression of β-defensin-2, β-defensin-3, and TLR-4 in male rats with apical periodontitis.","authors":"Leopoldo Cosme-Silva, Renan Dal-Fabbro, Fernanda de Lima Pontes, Leticia Cabrera Capalbo, Edilson Ervolino, Luciano Tavares Angelo Cintra, Juan José Segura-Egea, João Eduardo Gomes-Filho","doi":"10.1016/j.archoralbio.2024.106137","DOIUrl":"https://doi.org/10.1016/j.archoralbio.2024.106137","url":null,"abstract":"<p><strong>Objective: </strong>Evaluate whether a multi-strain probiotic formula affects blood parameters (hematologic, calcium, and phosphorus levels) and alters the expression of β-defensin-2, β-defensin-3, and toll-like receptor 4 in male rats with induced apical periodontitis (AP).</p><p><strong>Design: </strong>Wistar rats were divided into two groups (n = 8 each): (1) rats with AP on a regular diet (Control) and (2) rats with AP on a regular diet supplemented with the multi-strain probiotic GNC Probiotic Complex (GCP) at one billion CFU. AP was induced by exposing the dental pulp of the first molars to the oral environment. GCP was administered daily via gavage for 30 days during AP development. After 30 days, animals were anesthetized, a cardiac puncture was performed, and 5 mL of blood was collected for hematologic, calcium, and phosphorus analysis. Animals were then euthanized, and mandibles were removed for histological and immunochemical analysis of β-defensin-2, β-defensin-3, and toll-like receptor 4. Statistical analyses used Mann-Whitney U and Student's t-tests, with significance at P < 0.05.</p><p><strong>Results: </strong>No significant differences were observed in blood parameters between the Control and GCP groups (P > 0.05). In AP, the Control group showed more intense inflammatory infiltrates and higher median severity scores than the GCP group (P < 0.05). Immunoreactivity levels for β-defensin-2, β-defensin-3, and toll-like receptor 4 were significantly increased in the GCP group (P < 0.05).</p><p><strong>Conclusion: </strong>Probiotic complex reduces inflammation and enhances immunolabeling of β-defensin-2, β-defensin-3, and toll-like receptor 4 in AP.</p>","PeriodicalId":93882,"journal":{"name":"Archives of oral biology","volume":"170 ","pages":"106137"},"PeriodicalIF":0.0,"publicationDate":"2024-11-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142792925","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"m6A-related genes ALKBH5 and RBMX as prognostic and progression biomarkers in Chinese oral squamous cell carcinoma patients.","authors":"Yong Liu, Jiaying Nie, Ying Huang, Yunyan Yang, Wenen Su, Yumei Zhang, Zhuoqiao Gao, Shaohui Deng, Meilin Li, Shaoyan Lian, Jieying Li, Chaoqun Liu","doi":"10.1016/j.archoralbio.2024.106149","DOIUrl":"https://doi.org/10.1016/j.archoralbio.2024.106149","url":null,"abstract":"<p><strong>Objective: </strong>N6-methyladenosine (m6A) RNA dysregulation is crucial for cancer development. The study aimed to explore the effects of m6A modification in oral squamous cell carcinoma (OSCC) and its potential as a biomarker and therapeutic target.</p><p><strong>Design: </strong>We first analyzed m6A-related gene expression and its impact on OSCC prognosis and progression using the TCGA database. Subsequently, a Chinese cohort of 134 samples was used for validation. Bioinformatics analysis was conducted with TCGA data, and m6A levels were measured in the validation cohort using a quantification kit. Survival analysis was performed to study the relationship between m6A-related genes and OSCC prognosis in the Chinese population. The expression of m6A-related genes was assessed by using quantitative real-time PCR, Western blot analysis, and immunohistochemistry.</p><p><strong>Results: </strong>In the TCGA database, we found dysregulated expressions of METTL14, ALKBH5, YTHDF2, HNRNPC, LRPPRC, HNRNPA2B1, IGF2BP2, and RBMX in OSCC. Based on this, we observed significantly elevated total m6A content in OSCC tissues compared to normal controls in the validation cohort. Among the m6A candidate genes, only ALKBH5 and RBMX upregulation were found to be independent prognostic risk factors for poor OSCC survival in the Chinese population. And the inclusion of these two genes had a higher area under the curve for 3-year (0.705, 0.826), and 5-year (0.715, 0.788) overall survival compared to the model that only considered clinical parameters.</p><p><strong>Conclusions: </strong>We found the upregulation of m6A status in OSCC, of which, ALKBH5 and RBMX may serve as promising diagnostic and prognostic biomarkers for Chinese patients with OSCC.</p>","PeriodicalId":93882,"journal":{"name":"Archives of oral biology","volume":"170 ","pages":"106149"},"PeriodicalIF":0.0,"publicationDate":"2024-11-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142792882","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Tensile stress induced osteogenesis of periodontal ligament cells via Piezo1 mediated TAZ-Cbfα1 signaling.","authors":"Sisi Li, Ting Jin, Yi Wang, Hui Deng, Rongdang Hu","doi":"10.1016/j.archoralbio.2024.106152","DOIUrl":"https://doi.org/10.1016/j.archoralbio.2024.106152","url":null,"abstract":"<p><strong>Objective: </strong>Cyclic tensile stress (CTS) is known to induce osteogenesis of periodontal ligament cells (PDLCs), in which the molecular mechanism remains to be elucidated. This study aimed to investigate the role of the mechanosensitive calcium channel Piezo1 in the osteogenesis of PDLCs under tensile strain, as well as the signal regulation of the TAZ-Cbfα1 pathway in this process.</p><p><strong>Design: </strong>PDLCs were isolated from periodontal ligament tissues and subjected to CTS. Alizarin red staining (ARS) and alkaline phosphatase (ALP) assay were used to detect the osteogenesis of PDLCs. RT-qPCR and Western blot were used to detect the transcripts and protein expression levels of Piezo1, Transcriptional co-activator with PDZ binding motif (TAZ), and Core-binding factor α1 (Cbfα1) respectively. Immunofluorescence staining was used to detect the nuclear aggregation of TAZ. Small interfering RNA (siRNA) targeting Piezo1 (Piezo1-siRNA) was adopted to inhibit Piezo1 mRNA expression.</p><p><strong>Results: </strong>The results showed that the osteogenic differentiation capacity of PDLCs was significantly enhanced under CTS, along with elevated mRNA and protein expression levels of Piezo1, TAZ, and Cbfα1. Moreover, the ALP activity and the formation of calcium nodules by ARS staining were significantly increased. In addition, Piezo1 siRNA infection significantly inhibited the CTS-induced TAZ-Cbfα1 pathway and the osteogenesis of PDLCs, suggesting the regulatory role of Piezo1.</p><p><strong>Conclusions: </strong>We provided evidence that the application of CTS encourages the osteogenic differentiation of PDLCs, which could be mediated by the Piezo1 targeted TAZ-Cbfα1 signaling.</p>","PeriodicalId":93882,"journal":{"name":"Archives of oral biology","volume":"171 ","pages":"106152"},"PeriodicalIF":0.0,"publicationDate":"2024-11-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142815241","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Truncated recombinant Jagged1 fused with human IgG1 Fc activates Notch target genes in human periodontal ligament cells.","authors":"Y Nhu Tran, Ajjima Chansaenroj, Araya Jivaphetthai, Thanaphum Osathanon, Wanatchaporn Arunmanee","doi":"10.1016/j.archoralbio.2024.106138","DOIUrl":"https://doi.org/10.1016/j.archoralbio.2024.106138","url":null,"abstract":"<p><strong>Objective: </strong>Jagged1, a Notch ligand, is essential for osteogenic differentiation in human periodontal ligament cells (hPDLs) by interacting with Notch2 to induce osteogenic markers, alkaline phosphatase activity, and mineral deposition. However, its large size hampers absorption and distribution of biomaterials. This study aimed to identify the critical region of Jagged1 necessary for its interaction with Notch2 to create a truncated version that retains osteogenic activity but with improved delivery characteristics.</p><p><strong>Methods: </strong>Truncated versions of Jagged1 were designed by deleting C-terminal regions, focusing on the importance of the N-terminal domain. Both truncated and full-length Jagged1 were fused with human IgG1 Fc (Jagged1-Fc) and expressed in Chinese hamster ovary cells. hPDLs treated with these constructs were analyzed for Notch target gene expression using real-time PCR. Mineral deposition was assessed using alizarin red S staining.</p><p><strong>Results: </strong>Both truncated and full-length Jagged1-Fc increased the expression of Notch target genes (Hes1, Hey1, and ALP) in hPDLs, indicating successful activation of Notch signaling. However, only the full-length Jagged1-Fc enhanced mineral deposition, while the truncated version did not.</p><p><strong>Conclusions: </strong>Full-length Jagged1-Fc is required for mineral deposition and complete osteogenic differentiation in hPDLs. The truncated versions, while capable of activating Notch signaling, are ineffective in promoting mineralization, underscoring the importance of the entire protein for clinical applications in bone regeneration.</p>","PeriodicalId":93882,"journal":{"name":"Archives of oral biology","volume":"170 ","pages":"106138"},"PeriodicalIF":0.0,"publicationDate":"2024-11-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142792931","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antibacterial and antibiofilm potential of Thuja orientalis L. extract targeting cariogenic Enterococcus faecalis ATCC 29212: A combined in-vitro, in-silico study, and cytotoxicity screening.","authors":"Khyati Koul, Ishwerpreet Kaur Jawanda, Thomson Soni, Kashish Madaan, Sunidhi Bhatt, Pranjali Singh, Divyani Sharma, Sonia Bhonchal Bhardwaj, Seema Kumari","doi":"10.1016/j.archoralbio.2024.106107","DOIUrl":"https://doi.org/10.1016/j.archoralbio.2024.106107","url":null,"abstract":"<p><strong>Objectives: </strong>In this study, we explored the efficacy of methanolic extract of Thuja orientalis (TOME) as a novel antibacterial and antibiofilm agent against a cariogenic bacterium, Enterococcus faecalis ATCC 29212.</p><p><strong>Design: </strong>Antibacterial susceptibility studies were conducted and surface morphology analysis was performed using field emission scanning electron microscopy (FESEM). Antibiofilm activity was evaluated through both qualitative and quantitative biofilm inhibition assays and validated by microscopic analysis. In-silico molecular docking studies were conducted using the EDock server. The effectiveness of TOME was substantiated by biofilm model on dentin discs and cytotoxicity towards the HaCaT cell line was assessed using the MTT assay.</p><p><strong>Results: </strong>TOME exhibited significant bactericidal activity with minimum inhibitory concentration of 12.5 mg/mL and additionally, it effectively compromised bacterial cell wall integrity. Qualitative, quantitative and microscopic studies depicted the inhibition of biofilm formation. TOME significantly impacted the production of extracellular polymeric substance and extracellular DNA. Molecular docking studies identified beta-caryophyllene as a potent inhibitor of the Enterococcal surface protein (Esp). Biofilm model depicted the reduction of bacterial load on dentin discs. Additionally, TOME showed reduced cytotoxicity on HaCaT cells, indicating its potential as a safe therapeutic agent.</p><p><strong>Conclusion: </strong>These findings highlight TOME's promise for developing novel treatments for dental infections and biofilm-associated diseases. Further research should focus on isolating and characterizing the active compounds within TOME, particularly beta-caryophyllene, to elucidate their precise mechanisms of action.</p>","PeriodicalId":93882,"journal":{"name":"Archives of oral biology","volume":"171 ","pages":"106107"},"PeriodicalIF":0.0,"publicationDate":"2024-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142796736","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A novel mutation in GPR68 causes hypomaturation amelogenesis imperfecta","authors":"Shunlan Yu, Dandan Liu, Changqing Yan, Chao Yuan, Chenying Zhang, Shuguo Zheng","doi":"10.1016/j.archoralbio.2024.105991","DOIUrl":"https://doi.org/10.1016/j.archoralbio.2024.105991","url":null,"abstract":"","PeriodicalId":93882,"journal":{"name":"Archives of oral biology","volume":"111 6","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141033312","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}