Are hypoxia-related proteins associated with the invasiveness of glandular odontogenic cysts? A multicenter study

IF 2.2 4区医学 Q2 DENTISTRY, ORAL SURGERY & MEDICINE

Archives of oral biology Pub Date : 2024-11-30 DOI:10.1016/j.archoralbio.2024.106151

Rafaela de Albuquerque Dias , Karolyny Martins Balbinot , Karine Duarte da Silva , Ana Paula Neutzling Gomes , Carla Mosconi , Elismauro Franscisco de Mendonça , Sandra Beatriz Chaves Tarquinio , Sérgio de Melo Alves Junior , Maria Cássia Ferreira de Aguiar , João de Jesus Viana Pinheiro

{"title":"Are hypoxia-related proteins associated with the invasiveness of glandular odontogenic cysts? A multicenter study","authors":"Rafaela de Albuquerque Dias , Karolyny Martins Balbinot , Karine Duarte da Silva , Ana Paula Neutzling Gomes , Carla Mosconi , Elismauro Franscisco de Mendonça , Sandra Beatriz Chaves Tarquinio , Sérgio de Melo Alves Junior , Maria Cássia Ferreira de Aguiar , João de Jesus Viana Pinheiro","doi":"10.1016/j.archoralbio.2024.106151","DOIUrl":null,"url":null,"abstract":"<div><h3>Objective</h3><div>The study aimed to investigate the expression of hypoxia markers associated with invadopodia in glandular odontogenic cysts and to explore an association between this expression with the aggressive biological behaviour of this odontogenic cyst.</div></div><div><h3>Design</h3><div>Immunohistochemistry was employed to assess the expression of hypoxia-inducible factor 1 alpha (HIF-1α), notch homologous protein of the neurogenic locus 1 (NOTCH-1), disintegrin and metalloproteinase-12 (ADAM-12), and heparin-binding epidermal growth factor (HB-EGF) in 17 samples of glandular odontogenic cysts, 10 samples of calcifying odontogenic cysts, and 10 samples of dental follicles.</div></div><div><h3>Results</h3><div>The glandular odontogenic cyst samples exhibited increased expression of HIF-1α, NOTCH-1, ADAM-12 and HBEGF proteins compared with calcifying odontogenic cyst and dental follicle samples. HIF-1α demonstrated localization primarily within the nuclei of cystic epithelial cells of the glandular odontogenic cyst. NOTCH-1 and ADAM-12 exhibited expression in the cytoplasm and nuclei of epithelial and mucous cells of the glandular odontogenic cyst, of whereas HB-EGF was predominantly expressed in the cytoplasm. Weak labeling of these proteins was observed in the odontogenic epithelium of the calcifying odontogenic cyst and dental follicle samples.</div></div><div><h3>Conclusions</h3><div>The hypoxia-related signaling proteins are overexpressed in glandular odontogenic cyst when compared with calcifying odontogenic cyst and dental follicle. The reported aggressiveness of glandular odontogenic cyst can be partially explained by the expression of these proteins.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"171 ","pages":"Article 106151"},"PeriodicalIF":2.2000,"publicationDate":"2024-11-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Archives of oral biology","FirstCategoryId":"3","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0003996924002723","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"DENTISTRY, ORAL SURGERY & MEDICINE","Score":null,"Total":0}

引用次数: 0

Abstract

Objective

The study aimed to investigate the expression of hypoxia markers associated with invadopodia in glandular odontogenic cysts and to explore an association between this expression with the aggressive biological behaviour of this odontogenic cyst.

Design

Immunohistochemistry was employed to assess the expression of hypoxia-inducible factor 1 alpha (HIF-1α), notch homologous protein of the neurogenic locus 1 (NOTCH-1), disintegrin and metalloproteinase-12 (ADAM-12), and heparin-binding epidermal growth factor (HB-EGF) in 17 samples of glandular odontogenic cysts, 10 samples of calcifying odontogenic cysts, and 10 samples of dental follicles.

Results

The glandular odontogenic cyst samples exhibited increased expression of HIF-1α, NOTCH-1, ADAM-12 and HBEGF proteins compared with calcifying odontogenic cyst and dental follicle samples. HIF-1α demonstrated localization primarily within the nuclei of cystic epithelial cells of the glandular odontogenic cyst. NOTCH-1 and ADAM-12 exhibited expression in the cytoplasm and nuclei of epithelial and mucous cells of the glandular odontogenic cyst, of whereas HB-EGF was predominantly expressed in the cytoplasm. Weak labeling of these proteins was observed in the odontogenic epithelium of the calcifying odontogenic cyst and dental follicle samples.

Conclusions

The hypoxia-related signaling proteins are overexpressed in glandular odontogenic cyst when compared with calcifying odontogenic cyst and dental follicle. The reported aggressiveness of glandular odontogenic cyst can be partially explained by the expression of these proteins.

查看原文本刊更多论文

缺氧相关蛋白与腺性牙源性囊肿的侵袭性有关吗？多中心研究。

目的：研究与侵足相关的缺氧标志物在腺性牙源性囊肿中的表达，并探讨其表达与牙源性囊肿侵袭性生物学行为之间的关系。设计：采用免疫组化方法检测17例腺性牙源性囊肿、10例钙化牙源性囊肿和10例牙毛囊中缺氧诱导因子1α (HIF-1α)、神经源性位点1缺口同源蛋白（notch -1）、崩解素和金属蛋白酶-12 （ADAM-12）、肝素结合表皮生长因子（HB-EGF）的表达。结果：与钙化性牙源性囊肿和牙毛囊相比，腺样牙源性囊肿HIF-1α、NOTCH-1、ADAM-12和HBEGF蛋白表达增加。HIF-1α主要定位于腺性牙源性囊肿的囊性上皮细胞的细胞核内。NOTCH-1和ADAM-12在腺性牙源性囊肿上皮细胞和黏液细胞的细胞质和细胞核中表达，而HB-EGF主要在细胞质中表达。在钙化的牙源性囊肿和牙囊样本的牙源性上皮中观察到这些蛋白的弱标记。结论：与钙化性牙源性囊肿和牙毛囊相比，腺性牙源性囊肿中缺氧相关信号蛋白表达过高。这些蛋白的表达可以部分解释腺性牙源性囊肿的侵袭性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Archives of oral biology 医学-牙科与口腔外科

CiteScore

5.10

自引率

3.30%

发文量

177

审稿时长

26 days

期刊介绍： Archives of Oral Biology is an international journal which aims to publish papers of the highest scientific quality in the oral and craniofacial sciences. The journal is particularly interested in research which advances knowledge in the mechanisms of craniofacial development and disease, including: Cell and molecular biology Molecular genetics Immunology Pathogenesis Cellular microbiology Embryology Syndromology Forensic dentistry