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Enhanced regenerative potential of human dental pulp stem cells for the pulp-dentin complex through coculture with iPSC-derived endothelial cells: An in vitro study. 通过与ipsc来源的内皮细胞共培养增强人牙髓干细胞对牙髓-牙本质复合体的再生潜力：一项体外研究

IF 2.1

Archives of oral biology Pub Date : 2025-10-02 DOI: 10.1016/j.archoralbio.2025.106409

Hsu Myat Cho, Ukseong Kim, Sunil Kim, Stephanie Myeong Choi, Sukjoon Lee, Euiseong Kim

{"title":"Enhanced regenerative potential of human dental pulp stem cells for the pulp-dentin complex through coculture with iPSC-derived endothelial cells: An in vitro study.","authors":"Hsu Myat Cho, Ukseong Kim, Sunil Kim, Stephanie Myeong Choi, Sukjoon Lee, Euiseong Kim","doi":"10.1016/j.archoralbio.2025.106409","DOIUrl":"https://doi.org/10.1016/j.archoralbio.2025.106409","url":null,"abstract":"Objectives: Although cell-based therapies using human dental pulp stem cells (hDPSCs) with other cell lineages and growth factors show promise in regenerative endodontics, combining hDPSCs with induced pluripotent stem cell-derived endothelial cells (iPSC-ECs) is unexplored. Moreover, iPSC-ECs overcome ethical and practical challenges related to primary endothelial cells. This study explored the odontogenic and angiogenic potential of hDPSCs and iPSC-ECs in direct coculture.Design: hDPSCs were isolated from extracted human teeth, and iPSC‑ECs were generated via episomal reprogramming of hDPSCs followed by endothelial differentiation. Four groups were established for differentiation assays: hDPSCs in basal medium, osteogenic medium, modified osteogenic medium (D‑MOD), and coculture with iPSC‑ECs (1:5) in D‑MOD. Mineralization was assessed by alkaline phosphatase and alizarin red S staining; gene expression of odontogenic (DSPP, IBSP, ALPL) and angiogenic (PECAM1, MCAM, KDR) markers was measured by RT‑qPCR; protein levels were evaluated by Western blot and nestin immunofluorescence; and angiogenic capacity in the D‑MOD and coculture groups was quantified via Matrigel tube‑formation assay.Results: The coculture group showed enhanced mineralization and significantly increased expression of DSPP, IBSP, and PECAM1. Protein analysis confirmed elevated DSPP and nestin levels. Tube formation assays revealed significantly more junctions, segments, and meshes in the coculture group.Conclusions: This study demonstrated in vitro that coculturing hDPSCs with iPSC-ECs enhances both odontogenic and angiogenic differentiation compared to hDPSCs cultured alone. These findings highlight the potential of iPSC technology in regenerative endodontics and indicate a promising cell-based approach for future therapeutic applications.","PeriodicalId":93882,"journal":{"name":"Archives of oral biology","volume":"180 ","pages":"106409"},"PeriodicalIF":2.1,"publicationDate":"2025-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145245253","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ferulic acid suppresses Porphyromonas gingivalis biofilm formation via the inhibition of autoinducer-2 production and receptor activity. 阿魏酸通过抑制自诱导剂-2的产生和受体活性来抑制牙龈卟啉单胞菌生物膜的形成。

IF 2.1

Archives of oral biology Pub Date : 2025-10-01 Epub Date: 2025-07-26 DOI: 10.1016/j.archoralbio.2025.106365

Daiki Ando, Hnin Yu Lwin, Yukari Aoki-Nonaka, Aoi Matsugishi-Nasu, Yukako Minato, Yuko Warita, Naoki Takahashi, Koichi Tabeta

{"title":"Ferulic acid suppresses Porphyromonas gingivalis biofilm formation via the inhibition of autoinducer-2 production and receptor activity.","authors":"Daiki Ando, Hnin Yu Lwin, Yukari Aoki-Nonaka, Aoi Matsugishi-Nasu, Yukako Minato, Yuko Warita, Naoki Takahashi, Koichi Tabeta","doi":"10.1016/j.archoralbio.2025.106365","DOIUrl":"10.1016/j.archoralbio.2025.106365","url":null,"abstract":"Objective: This study aimed to clarify the antibacterial and antibiofilm effects of ferulic acid against periodontal pathogenic bacteria.Design: The cytotoxicity of ferulic acid was examined using the MTT assay on the human oral epithelial cell line Ca9-22. To determine the minimum inhibitory concentration (MIC) and minimum bactericidal concentration, Porphyromonas gingivalis ATCC 33277, Fusobacterium nucleatum ATCC 25586, Prevotella intermedia ATCC 25611, Aggregatibacter actinomycetemcomitans JP2, and Streptococcus mitis ATCC 903 were treated with ferulic acid. The inhibition of biofilm formation was evaluated by crystal violet staining. The inhibition of P. gingivalis autoinducer-2 (AI-2) production and receptor activity was evaluated by luminescence measurements using the sensor strain Vibrio harveyi BB170.Results: Ferulic acid did not exhibit any cytotoxicity on human oral epithelial cells. The MICs of ferulic acid against P. gingivalis and A. actinomycetemcomitans were 1000 and 500 µg/mL, respectively. It did not show antibacterial activity against F. nucleatum, P. intermedia, and S. mitis, indicating the weak antibacterial activity of ferulic acid. However, ferulic acid significantly inhibited P. gingivalis biofilm formation at low concentrations below 1/8 MIC. It specifically inhibited AI-2 production from P. gingivalis below 1/8 MIC and suppressed the receptor activity of AI-2.Conclusions: Although ferulic acid showed weak antibacterial activity against periodontopathogenic bacteria, it had low cytotoxicity and inhibited P. gingivalis biofilm formation. Ferulic acid inhibited AI-2 production and receptor activity, suggesting that ferulic acid is an efficient quorum-sensing inhibitor for controlling P. gingivalis biofilm formation.","PeriodicalId":93882,"journal":{"name":"Archives of oral biology","volume":"178 ","pages":"106365"},"PeriodicalIF":2.1,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144812780","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A novel mutation in GPR68 causes hypomaturation amelogenesis imperfecta GPR68的一种新型突变会导致发育不全髓质发育不全症

Archives of oral biology Pub Date : 2024-05-01 DOI: 10.1016/j.archoralbio.2024.105991

Shunlan Yu, Dandan Liu, Changqing Yan, Chao Yuan, Chenying Zhang, Shuguo Zheng

引用次数: 0