ACS ES&T waterPub Date : 2025-04-29eCollection Date: 2025-05-09DOI: 10.1021/acsestwater.4c01092
Trent Biggs, Natalie Mladenov, Stephany Garcia, Yongping Yuan, Daniel Sousa, Alexandra Grant, Elise Piazza, Trinity Magdalena-Weary, Callie Summerlin, Doug Liden
{"title":"Fluorescence-Based Indicators of <i>Escherichia coli</i> and Untreated Wastewater: Turbidity Correction and Comparison of <i>In Situ</i> and Benchtop Fluorometers in a Sewage-Polluted Urban River.","authors":"Trent Biggs, Natalie Mladenov, Stephany Garcia, Yongping Yuan, Daniel Sousa, Alexandra Grant, Elise Piazza, Trinity Magdalena-Weary, Callie Summerlin, Doug Liden","doi":"10.1021/acsestwater.4c01092","DOIUrl":"https://doi.org/10.1021/acsestwater.4c01092","url":null,"abstract":"<p><p>Fluorescence-based sensors of tryptophan (TRP) and chromophoric dissolved organic matter (CDOM) can provide real-time information on water quality. We 1) evaluate the accuracy of benchtop (Aqualog) and <i>in situ</i> (Manta) fluorometers for estimating percent untreated wastewater (pctWW) in laboratory experiments; 2) propose a correction for turbidity; 3) test for correlations between TRP or CDOM and <i>Escherichia coli</i> (<i>E. coli</i>) concentrations in river water samples; and 4) quantify the impact of stormflow on wastewater discharge and bacteria in the Tijuana River. In laboratory experiments, Aqualog TRP correlated closely with pctWW (R<sup>2</sup> > 0.98), while Manta TRP increased with pctWW up to 20-50% but then decreased thereafter. In river water samples, Aqualog TRP and CDOM had stronger correlations with <i>E. coli</i> concentrations than did Manta TRP, which correlates with <i>E. coli</i> but the relationship varies by event. Wastewater flow (m<sup>3</sup> s<sup>-1</sup>) increased during storm events, but much less than total storm discharge, so the percent wastewater decreased during storms. Benchtop fluorometers provide reliable estimates of wastewater percentage and bacteria concentrations, while <i>in situ</i> fluorometers indicate wastewater and bacteria presence, but may not provide reliable estimates in highly polluted waters or stormflow without further correction of turbidity, inner filter effects, and variations among storm events.</p>","PeriodicalId":93847,"journal":{"name":"ACS ES&T water","volume":"5 5","pages":"2212-2222"},"PeriodicalIF":4.8,"publicationDate":"2025-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12070408/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144082656","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
ACS ES&T waterPub Date : 2025-04-29eCollection Date: 2025-05-09DOI: 10.1021/acsestwater.4c01213
Panagiota Adamou, James Entwistle, David W Graham, Anke Neumann
{"title":"Mineral-Based Advanced Oxidation Processes for Enhancing the Removal of Antibiotic Resistance Genes from Domestic Wastewater.","authors":"Panagiota Adamou, James Entwistle, David W Graham, Anke Neumann","doi":"10.1021/acsestwater.4c01213","DOIUrl":"https://doi.org/10.1021/acsestwater.4c01213","url":null,"abstract":"<p><p>Wastewater treatment plants (WWTPs) release antibiotic-resistant bacteria (ARB) and antibiotic-resistant genes (ARGs) into the environment. Advanced oxidation processes (AOPs) can remove ARB and ARGs, but they often require impractically high chemical or energy use. Here, we explore a low-energy AOP that uses Fe-bearing clay mineral (NAu-1) either combined with H<sub>2</sub>O<sub>2</sub> (H<sub>2</sub>O<sub>2</sub>/NAu-1) or as prereduced structural Fe (rNAu-1) to degrade selected ARGs (i.e., <i>tet</i>M, <i>tet</i>Q, and <i>bla</i> <sub>OXA-10</sub>), <i>int</i>1 (a mobile genetic element), and the 16S rRNA gene in postsecondary WWTP effluents. Addition of H<sub>2</sub>O<sub>2</sub>/NAu-1 significantly increased <i>tet</i>M and <i>int</i>1 removals relative to UV irradiation and H<sub>2</sub>O<sub>2</sub>/UV (<i>p</i> ≤ 0.02). Removals increased with greater H<sub>2</sub>O<sub>2</sub> doses and contact times, reaching maximum values of 1.2 and 2.3 log units at H<sub>2</sub>O<sub>2</sub> doses of 0.26 and 10 mM and contact times of 4 and 8 h, respectively. Bacterial regrowth after 24 h of contact was probably due to H<sub>2</sub>O<sub>2</sub> depletion. However, the addition of rNAu-1 achieved the highest removals, up to 2.9 log units after 0.5 h, and suppressed bacterial regrowth over 24 h. Similar removals were observed with rNAu-1 under oxic and anoxic conditions. Results show that mineral-based AOPs offer the potential for elevated ARG removal and lower chemical and energy demands in tertiary wastewater treatment.</p>","PeriodicalId":93847,"journal":{"name":"ACS ES&T water","volume":"5 5","pages":"2310-2321"},"PeriodicalIF":4.8,"publicationDate":"2025-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12070418/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144082719","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
ACS ES&T waterPub Date : 2025-04-28DOI: 10.1021/acsestwater.4c0111310.1021/acsestwater.4c01113
Catherine S. Moody*, Nicholle G. A. Bell, C. Logan Mackay and Ezra Kitson,
{"title":"Spatial and Temporal Variations in Aquatic Organic Matter Composition in UK Surface Waters","authors":"Catherine S. Moody*, Nicholle G. A. Bell, C. Logan Mackay and Ezra Kitson, ","doi":"10.1021/acsestwater.4c0111310.1021/acsestwater.4c01113","DOIUrl":"https://doi.org/10.1021/acsestwater.4c01113https://doi.org/10.1021/acsestwater.4c01113","url":null,"abstract":"<p >Drinking water is becoming more difficult to treat, especially in the UK, due to the changing concentration and composition of aquatic dissolved organic matter (DOM). The spatial and temporal variations in the DOM composition are not well understood. This study investigated how DOM composition varies along a north/south gradient in the UK, over four years, and between headwaters and reservoirs. There were trends in DOM composition metrics from north to south; carbohydrate and peptide-like compounds were lower in northern sites, while lipid-like compounds were lower further south, suggesting different sources of DOM in north/south catchments. DOM collected in Autumn 2021, after a Summer of low rainfall, was more aromatic, less oxidized, and more diverse than DOM collected in 2018–2020. Decreased lipid content and increased oxy-aromatic content occurred in Autumn, at the end of the plant growing season, when increased rainfall rewets catchments and mobilizes soil OM into surface waters. These seasonal changes in DOM composition coincide with increased DOM concentrations in raw drinking water, leading to more challenges for drinking water treatment, especially as climate change alters rainfall distribution in the UK.</p><p >Understanding natural variations in organic matter composition is important for UK drinking water treatment. This study used four years of data to identify trends in organic matter composition with latitude, climate, and time of year.</p>","PeriodicalId":93847,"journal":{"name":"ACS ES&T water","volume":"5 5","pages":"2233–2243 2233–2243"},"PeriodicalIF":4.8,"publicationDate":"2025-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.acs.org/doi/epdf/10.1021/acsestwater.4c01113","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143921380","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
ACS ES&T waterPub Date : 2025-04-28DOI: 10.1021/acsestwater.5c0007310.1021/acsestwater.5c00073
Ian J. Vander Meulen, Jason M. E. Ahad, Danna M. Schock, Lukas J. Mundy, Bruce D. Pauli, Dena W. McMartin and John V. Headley*,
{"title":"Exploring Surface Water-Bitumen Interactions in Athabasca Oil Sands Wetlands Using Stable and Radiocarbon Isotopes","authors":"Ian J. Vander Meulen, Jason M. E. Ahad, Danna M. Schock, Lukas J. Mundy, Bruce D. Pauli, Dena W. McMartin and John V. Headley*, ","doi":"10.1021/acsestwater.5c0007310.1021/acsestwater.5c00073","DOIUrl":"https://doi.org/10.1021/acsestwater.5c00073https://doi.org/10.1021/acsestwater.5c00073","url":null,"abstract":"<p >Naphthenic acid fraction compounds (NAFCs) have been identified as aquatic contaminants of concern associated with bitumen extraction in Canada’s Athabasca oil sands region. Previous investigations found that NAFCs occur and degrade in young (<100-year-old) wetlands, but the degree to which these NAFCs might be bitumen-derived remains unclear. To quantify contributions of modern versus fossil NAFCs, carbon isotope-based methods were applied to two intensively sampled wetlands suspected to be influenced by oil sands. Carbon-13 isotope ratios measured by pyrolytic decarboxylation (δ<sup>13</sup>C<sub>pyr</sub>) fell within a narrow range (−27.5 to −26.0‰) and radiocarbon measurements (Δ<sup>14</sup>C) revealed a significant fossil carbon contribution (−795 to −290‰), presumably from bitumen. Along the flow pathway of one wetland, a positive shift of ∼1.5‰ in δ<sup>13</sup>C<sub>pyr</sub> correlated with increasing distance from a suspected oil sands point source. Lower Δ<sup>14</sup>C values (i.e., less <sup>14</sup>C) at this site corresponded to lighter δ<sup>13</sup>C<sub>pyr r</sub> values – the opposite trend found in previous applications of δ<sup>13</sup>C<sub>pyr</sub> analyses, whereby bitumen-derived NAFSs were around −22 to −21‰. This study shows how source attribution of NAFCs in surface water using δ<sup>13</sup>C<sub>pyr</sub> may be less straightforward compared to groundwater, possibly due to differences in natural attenuation processes between these two environments.</p><p >Carbon isotope-based forensic techniques applied in the Athabasca oil sands region wetlands generated contrasting results, highlighting ambiguities in bitumen carbon mobility.</p>","PeriodicalId":93847,"journal":{"name":"ACS ES&T water","volume":"5 5","pages":"2512–2520 2512–2520"},"PeriodicalIF":4.8,"publicationDate":"2025-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.acs.org/doi/epdf/10.1021/acsestwater.5c00073","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143921401","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
ACS ES&T waterPub Date : 2025-04-28DOI: 10.1021/acsestwater.5c0010410.1021/acsestwater.5c00104
William Chen, and , Kyle Bibby*,
{"title":"Temporal, Spatial, and Methodological Considerations in Wastewater-Based Epidemiology for Sexually Transmitted Infections","authors":"William Chen, and , Kyle Bibby*, ","doi":"10.1021/acsestwater.5c0010410.1021/acsestwater.5c00104","DOIUrl":"https://doi.org/10.1021/acsestwater.5c00104https://doi.org/10.1021/acsestwater.5c00104","url":null,"abstract":"<p >Sexually transmitted infections (STIs) result in millions of cases and cost over $16 billion annually in the U.S. High asymptomatic rates and stigma hinder timely diagnosis and elimination efforts. Wastewater-based epidemiology (WBE) offers a potential solution through noninvasive surveillance, facilitating earlier detection and response than clinical surveillance alone. Here, we use a model-based approach to evaluate the suitability of STIs for WBE. Human papillomavirus (HPV) is the most suitable STI for WBE, requiring 1–3 PCR replicates at a maximum process limit of detection (PLOD) of 5.0 log10 genome copies (GC)/L to achieve a 50% detection rate, due to its high median shedding rate and prevalence. Oncogenic HPV-16 and 18 are also highly detectable in wastewater in all countries analyzed using three PCR replicates at the minimum PLOD. Chlamydia shows strong WBE potential, detectable in 90% of countries analyzed, while <i>Mycoplasma genitalium</i>, hepatitis B, and gonorrhea require more PCR replicates under similar conditions. Hepatitis C WBE is predicted to be challenging, requiring a 10–300-fold increase in disease prevalence for feasible wastewater detection in most countries. Reducing the PLOD to 2.6–3.6 log10 GC/L and sampling at STI hotspots are critical for enhancing WBE detection capabilities.</p><p >We evaluate the potential of applying WBE to six STIs, identifying when, where, and how it can be successfully applied.</p>","PeriodicalId":93847,"journal":{"name":"ACS ES&T water","volume":"5 5","pages":"2533–2546 2533–2546"},"PeriodicalIF":4.8,"publicationDate":"2025-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143921385","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
ACS ES&T waterPub Date : 2025-04-27DOI: 10.1021/acsestwater.5c0025110.1021/acsestwater.5c00251
Ziyi Wang, Zhengjian Yang, Jiahui Shang, Longfei Wang* and Yi Li*,
{"title":"Bistable States of Microbial Communities Driven by Nutrient Loading in the Hyporheic Zone of Effluent-Dominated Rivers: Predicting Taxonomic Composition and Metabolic Functions","authors":"Ziyi Wang, Zhengjian Yang, Jiahui Shang, Longfei Wang* and Yi Li*, ","doi":"10.1021/acsestwater.5c0025110.1021/acsestwater.5c00251","DOIUrl":"https://doi.org/10.1021/acsestwater.5c00251https://doi.org/10.1021/acsestwater.5c00251","url":null,"abstract":"<p >Effluent discharge from wastewater treatment plants alters nitrogen and sulfur cycling in the hyporheic zone (HZ), potentially shifting microbial communities to alternative stable states. However, these transitions remain poorly understood in such specific subsurface environments. Here, we characterized and predicted multiple stable states of communities in the HZ of representative effluent-dominated rivers by integrating molecular techniques, alternative stable states theory, and machine learning models. The results revealed the existence of bistable states in terms of microbial taxa, functional genes, and metabolic pathways. The potential analysis demonstrated that with increases in nitrogen and sulfur loading, the taxonomic composition shifted from a state with higher diversity and lower stability to one with more prominent interspecific competition. The regime shift in metabolic functions was likely the initial transformation, as it was subsequently followed by alterations in the taxonomic composition. Optimized random forest and XGBoost models combined with network embedding achieved over 90% accuracy in predicting taxonomic composition and metabolic functions, outperforming stand-alone machine learning models. The generated results demonstrated that the accurate description and prediction of microbial responses to anthropogenic disturbances, e.g., effluent discharge, required the joint evaluation of variability in community structure and metabolic function.</p>","PeriodicalId":93847,"journal":{"name":"ACS ES&T water","volume":"5 5","pages":"2672–2683 2672–2683"},"PeriodicalIF":4.8,"publicationDate":"2025-04-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143921247","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
ACS ES&T waterPub Date : 2025-04-26DOI: 10.1021/acsestwater.5c0013810.1021/acsestwater.5c00138
Peilian Zhang, Hanxiao Zhang*, Zhanyao Shi, Weihui Huang, Jing Wang and Shouliang Huo*,
{"title":"Microbial Control on Dissolved Organic Matter Composition along the South-to-North Water Diversion Middle Route Project","authors":"Peilian Zhang, Hanxiao Zhang*, Zhanyao Shi, Weihui Huang, Jing Wang and Shouliang Huo*, ","doi":"10.1021/acsestwater.5c0013810.1021/acsestwater.5c00138","DOIUrl":"https://doi.org/10.1021/acsestwater.5c00138https://doi.org/10.1021/acsestwater.5c00138","url":null,"abstract":"<p >Drinking water safety is vital in large-scale water transfer systems such as the South-to-North Water Diversion Middle Route Project (SNWDP-M). Dissolved organic matter (DOM) significantly affects water quality, although factors driving its compositional changes along the SNWDP-M remain insufficiently explored. This study integrated DOM spectral analyses and ultrahigh resolution mass spectrometry with 16S rRNA gene sequencing to explore DOM–bacterial community (BC) interplay. Results demonstrated that the midstream section (MS) of the SNWDP-M exhibited higher protein-like content compared to the upstream section (US) and downstream section (DS), while the DS had relatively higher humic-like content. DOM molecular complexity and aromaticity increased from US to DS was observed, alongside a rise in bacterial diversity. Bipartite network analysis revealed evolving DOM-BC interactions, with generalized metabolic interactions DS, where BCs exerting a stronger influence on DOM composition downstream (RDA, <i>R</i><sup>2</sup> = 0.62) compared to the US (<i>R</i><sup>2</sup> = 0.29) and MS (<i>R</i><sup>2</sup> = 0.24). It highlights enhanced microbial control in the DS, emphasizing the need to integrate microbial dynamics into water quality management. Taken together, this study elucidates the dynamic DOM processes along the SNWDP-M and the BC influence, providing a basis for improving water quality management in large-scale water transfer projects.</p>","PeriodicalId":93847,"journal":{"name":"ACS ES&T water","volume":"5 5","pages":"2568–2580 2568–2580"},"PeriodicalIF":4.8,"publicationDate":"2025-04-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143921239","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
ACS ES&T waterPub Date : 2025-04-26DOI: 10.1021/acsestwater.4c0118010.1021/acsestwater.4c01180
Emine N. Fidan*, Brian J. Reich, Ryan E. Emanuel, Angela Harris, Sophia Kathariou and Natalie G. Nelson*,
{"title":"Spatiotemporal Dynamics and Drivers of Microbial Contaminants in Hurricane Florence Floodwaters","authors":"Emine N. Fidan*, Brian J. Reich, Ryan E. Emanuel, Angela Harris, Sophia Kathariou and Natalie G. Nelson*, ","doi":"10.1021/acsestwater.4c0118010.1021/acsestwater.4c01180","DOIUrl":"https://doi.org/10.1021/acsestwater.4c01180https://doi.org/10.1021/acsestwater.4c01180","url":null,"abstract":"<p >To strengthen our understanding regarding the signatures and drivers of floodwater contamination, this study aimed to investigate spatiotemporal patterns of pathogens, fecal indicator bacteria, and fecal biomarker detections, and identify the watershed characteristics that best explain water quality signatures in floodwaters. To accomplish this, we collected water samples across 51 sites during and after Hurricane Florence impacted North Carolina in 2018. Each site was visited four times, and samples were assessed for <i>Arcobacter</i>, <i>Salmonella</i>, <i>Listeria</i>, <i><i>Escherichia coli</i></i>, and source-specific biomarkers HF183 and Pig2Bac. Water quality responses were explained using a multivariate Bayesian logistic regression model incorporating land characteristics, pollution point sources, and hydroclimatic factors contributing to water quality degradation. Model results suggested that during flood conditions, pollution point sources were the dominant contributors to surface water contamination, likely due to direct connectivity with floodwaters. In contrast, nonpoint sources and rainfall-driven processes played a greater role in pollutant transport during nonflooded conditions. Overall, the high prevalence of contaminants during flood conditions underscores public health concerns associated with floodwater exposure. In particular, modeling results reveal potential drivers and sources of water quality contamination across spatiotemporal scales and can inform targeted strategies for improved water quality management and enhanced public health protection.</p>","PeriodicalId":93847,"journal":{"name":"ACS ES&T water","volume":"5 5","pages":"2255–2267 2255–2267"},"PeriodicalIF":4.8,"publicationDate":"2025-04-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143921240","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
ACS ES&T waterPub Date : 2025-04-24DOI: 10.1021/acsestwater.5c0015210.1021/acsestwater.5c00152
Tingting Zhu, Zhiwen Wang, Jiazeng Ding, Yan Gong, Jiaxing Huang, Yufen Wang and Yiwen Liu*,
{"title":"The Biochar Pretreatment Addition Coupled with Codigestion for Facilitating Methane Production from Anaerobic Digestion: Performance and Mechanisms","authors":"Tingting Zhu, Zhiwen Wang, Jiazeng Ding, Yan Gong, Jiaxing Huang, Yufen Wang and Yiwen Liu*, ","doi":"10.1021/acsestwater.5c0015210.1021/acsestwater.5c00152","DOIUrl":"https://doi.org/10.1021/acsestwater.5c00152https://doi.org/10.1021/acsestwater.5c00152","url":null,"abstract":"<p >A new technology using biochar pretreatment coupled with codigestion (BPC) to increase methane production of waste-activated sludge (WAS) in anaerobic digestion (AD) was introduced in this study. The findings indicated that BPC dramatically increased methane production with a maximum cumulative methane production of 74.34 L CH<sub>4</sub>/kg VS at 2.0 g biochar/g VS pretreatment, which increased by 57.6% compared to the control. Model fitting analysis revealed that BPC significantly promoted both the reaction rate and the biochemical methane potential (BMP). In the pretreatment process, the high alkalinity and stable pH buffer ability of biochar further enhance the release of organics, facilitating subsequent methanogenic fermentation. In the AD process, oxygen-containing functional groups such as C═O and C–OH, related to redox properties, play an essential role in augmenting methane generation. This might be attributed to the biochar improving the direct interspecies electron transfer from methanogenic bacteria through conductive material. Microbial community analysis demonstrated that the functional microorganisms that participated in the AD process were enriched from 28.16% in the control to 35.15 and 35.45% in traditional biochar addition and BPC group, respectively. It will be clear for the application of BPC technology to enhance methane yield.</p>","PeriodicalId":93847,"journal":{"name":"ACS ES&T water","volume":"5 5","pages":"2605–2618 2605–2618"},"PeriodicalIF":4.8,"publicationDate":"2025-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143921374","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}