Journal of chromatography open最新文献

{"title":"Editorial on “Reviews 2025” Virtual Special Issue","authors":"Salvatore Fanali ((Editor-in-Chief of the Journal of Chromatography Open))","doi":"10.1016/j.jcoa.2026.100341","DOIUrl":"10.1016/j.jcoa.2026.100341","url":null,"abstract":"","PeriodicalId":93576,"journal":{"name":"Journal of chromatography open","volume":"9 ","pages":"Article 100341"},"PeriodicalIF":3.2,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148231047","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sustainable methods for flavonoid determination: Applications with modern green analytical techniques","authors":"Seyda Yayla ,&nbsp;Ahmet Cetinkaya ,&nbsp;M. Mesud Hurkul ,&nbsp;Sibel A. Ozkan","doi":"10.1016/j.jcoa.2026.100310","DOIUrl":"10.1016/j.jcoa.2026.100310","url":null,"abstract":"<div><div>Flavonoids are a diverse group of polyphenolic compounds. They are ubiquitously found in fruits, vegetables, tea, and medicinal plants. They play a pivotal role in both plant physiology and human health. Numerous studies have demonstrated that these compounds exhibit a wide range of biological activities, including antioxidant, anti-inflammatory, cardioprotective, neuroprotective, and anticancer properties. Accordingly, significant research attention has been directed toward these compounds in nutraceutical, pharmaceutical, and food science research. Evidence is growing that a diet rich in flavonoids can help to prevent and manage several chronic diseases, including cardiovascular disorders, diabetes, neurodegenerative diseases, and certain cancers. However, the accurate quantification and characterisation of flavonoids remain a scientific challenge. This is due to their structural diversity, low stability, and matrix complexity in natural samples. Conventional analytical approaches, while widely used for their effectiveness, raise environmental concerns due to their reliance on toxic solvents and the resulting hazardous waste, which runs counter to sustainable chemistry principles. The escalating ecological and regulatory demands underscore the imperative to promptly implement green analytical methodologies that are both analytically robust and ecologically compatible. The present review focuses on studies published within the past five years. In recent years, modern green analytical techniques have reshaped approaches to phytochemical analysis by prioritizing sustainability alongside analytical performance. Examples of these techniques include supercritical fluid chromatography (SFC), microextraction techniques, capillary electrophoresis, and miniaturised spectrometric systems. These methods follow the principles of Green Analytical Chemistry (GAC) by using fewer reagents, reducing analysis time, and consuming less energy. Also, the use of combined methods (e.g., LC-MS/MS, GC–MS, or HPLC-DAD) with microfluidic systems and biosensors has created new possibilities for fast, portable measurement of flavonoids. This review provides a comprehensive examination of recent advances in green analytical techniques, emphasizing their methodological innovations and practical applications. By critically evaluating these developments, the work contributes to the ongoing transformation of natural product analysis, fostering approaches that are not only more sustainable but also resource-efficient and aligned with contemporary environmental imperatives. Moreover, it underscores the pivotal role of green analytical methodologies in improving analytical performance while minimising ecological impact.</div></div>","PeriodicalId":93576,"journal":{"name":"Journal of chromatography open","volume":"9 ","pages":"Article 100310"},"PeriodicalIF":3.2,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146172856","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Applicability of wide-pore columns in reversed-phase liquid chromatography for human blood lipid profiling","authors":"Sergey Girel ,&nbsp;Davy Guillarme ,&nbsp;Serge Rudaz ,&nbsp;Isabel Meister","doi":"10.1016/j.jcoa.2025.100295","DOIUrl":"10.1016/j.jcoa.2025.100295","url":null,"abstract":"<div><div>For the resolution of isomer-rich lipid extracts, it is common practice during RPLC method development to evaluate column efficiency and mobile phase composition. However, further gains can be made by tuning other parameters, such as the microstructure of the packing material. Today, lipid separations are mostly performed with columns packed with particles having narrow pore sizes (80–120 Å). This is unsurprising, given the expected hydrodynamic radius of lipids up to of 23 Å. However, specific investigations of pore size influence on lipid separations are scarce. Therefore, a systematic comparison of 150 mm columns packed with C₁₈ particles having pore sizes of 130 and 300 Å was performed with respect to selectivity, peak capacity and chromatographic resolution. An RPLC/HRMS-based lipidomic analysis was developed on 150 mm columns. Similar loadability, selectivity, sensitivity and average peak capacity were demonstrated by 300 Å column compared to its narrow-pore counterpart. Overall, current experimental results indicate that wide-pore columns are equally applicable for RPLC/HRMS-based discovery lipidomics and may be even beneficial for the analysis of lipids with high structural complexity. In addition, these columns appear to be better suitable for high-throughput analysis due to lower lipid retention via smaller surface area.</div></div>","PeriodicalId":93576,"journal":{"name":"Journal of chromatography open","volume":"9 ","pages":"Article 100295"},"PeriodicalIF":3.2,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145684633","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Magnetic polyamidoamine dendrimer with thiol terminal groups for magnetic solid-phase extraction of selected organophosphorus pesticides followed by quantification through high-performance liquid chromatography-ultraviolet detection","authors":"Milad Ghani ,&nbsp;Seyedeh Bentolhoda Hosseinian ,&nbsp;Behrooz Maleki ,&nbsp;Pouya Ghamari kargar","doi":"10.1016/j.jcoa.2026.100306","DOIUrl":"10.1016/j.jcoa.2026.100306","url":null,"abstract":"<div><div>Herein, polyamidoamine-SH dendrimer@Fe₃O₄ (PAMAM-SH@Fe₃O₄) was synthesized using a simple, green, fast, cost-effective, and efficient method. Firstly, polyamidoamine dendrimer with SH terminal groups (PAMAM-SH) was prepared by the Michael addition method. Subsequently, PAMAM-SH was immobilized on the surface of Fe₃O₄ nanoparticles. The proposed PAMAM-SH@Fe₃O₄ material exhibits superior extraction efficiency compared to conventional sorbents due to its abundant functional groups and thiol modifications, enabling highly effective MSPE of organophosphorus pesticides in complicated matrices. The obtained PAMAM-SH@Fe₃O₄ nanocomposite was utilized as a sorbent for magnetic solid-phase extraction (MSPE) of four selected organophosphorus pesticides (OPPs), including diazinon, malathion, chlorpyrifos, and phosalone. The extracted analytes were then desorbed using methanol (as the appropriate eluent) and analyzed by high-performance liquid chromatography with ultraviolet detection (HPLC-UV). Under optimized condition, the method exhibited good linearity in the range of 0.10–500.00 µg L⁻¹ for the target analytes (corresponding coefficients of determination (R²) &gt; 0.9923). The limits of detection (LODs) and quantification (LOQs) were in the ranges of 0.02–0.13 µg L⁻¹ and 0.08–0.42 µg L⁻¹, respectively. Inter- and intra-day relative standard deviations (RSDs) were between 1.60% and 5.90%. The enrichment factors (EFs) were determined to be 79.00, 84.00, 72.00, and 81.00 for diazinon, malathion, chlorpyrifos, and phosalone, respectively. The proposed method was successfully applied to real samples, including apples, pears, peaches, grapes, and agricultural wastewater, demonstrating its reliability and applicability for complex matrices.</div></div>","PeriodicalId":93576,"journal":{"name":"Journal of chromatography open","volume":"9 ","pages":"Article 100306"},"PeriodicalIF":3.2,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145924373","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of simplified method for the detection of capsaicin using high performance liquid chromatography","authors":"Jiang Huo ,&nbsp;J. Heinrich Lieth ,&nbsp;Qi Zhang ,&nbsp;Pramod Pandey","doi":"10.1016/j.jcoa.2025.100296","DOIUrl":"10.1016/j.jcoa.2025.100296","url":null,"abstract":"<div><div>Peppers are widely used for their pungency, and Scoville Heat Unit (SHU) is often used to measure the pungency of the peppers caused by compounds called capsaicinoids. Liquid Chromatography (LC) methods have emerged as effective tools for determining capsaicinoids. However, accurate extraction and quantification of capsaicin remain challenging. Here, we developed a simple, rapid, and robust high-performance liquid chromatography-ultraviolet detector (HPLC-UV) method for quantifying capsaicin in green Thai pepper (Bird’s eye chili). Capsaicin separation was achieved using a SiELC Newcrom R1 column (100 × 3.2 mm, 3 µm) under isocratic mobile phase consisting of 0.5 % formic acid in water and acetonitrile (30 %:70 %) at a flow rate of 0.4 mL/min. Detection of capsaicin was done at 220 nm and 280 nm wavelengths. Capsaicin extraction from pepper samples was evaluated at 20 min, 60 min, and 120 min intervals. Results showed that a 60-min extraction at 60 °C with orbital mixing 150 rpm was sufficient to recover the majority of the capsaicin from green Thai pepper. The method showed excellent linearity across 11 concentration levels (0.5 - 600 ppm) with an R² value of 0.99. While capsaicin analysis has been investigated previously, this streamlined HPLC-UV method provides an accessible and effective alternative for improving capsaicin extraction and detection in green chili peppers.</div></div>","PeriodicalId":93576,"journal":{"name":"Journal of chromatography open","volume":"9 ","pages":"Article 100296"},"PeriodicalIF":3.2,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145924374","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of metal oxide-based solid phase Microextraction fiber for the determination of volatile organic compounds","authors":"Berfu Yığrık ,&nbsp;Duygu Oğuz Kılıç ,&nbsp;Levent Pelit","doi":"10.1016/j.jcoa.2026.100320","DOIUrl":"10.1016/j.jcoa.2026.100320","url":null,"abstract":"<div><div>Volatile organic compounds (VOCs) have high vapor pressures and readily evaporate at room temperature, thereby accumulating in the indoor environment, so their levels frequently exceed outdoor levels. Due to the harmful effects of many VOCs, such as benzene, toluene, ethylbenzene and xylenes (BTEX) on human health, effective monitoring of these compounds in the indoor environment is quite important. The conventional techniques are often poor sensitivity, costly and necessitate the expertise of specialists or the procurement of sophisticated equipment. Therefore, the development of rapid, sensitive, cost-effective, and straightforward analytical methods for the monitoring of them in indoor environments is required. In this study, a fast, easy, cost-effective, and sensitive gas chromatographic method based on the metal oxide solid phase microextraction (SPME) fiber was developed for the monitoring of BTEX in the indoor environment. The performance of different metal oxide coatings, such as TiO₂, RuO₂, and IrO₂, was tested. The best BTEX extraction efficiencies were obtained by IrO₂-SPME-Fiber. To get the best extraction efficiency, IrO₂-SPME-Fiber coating conditions, including Ir, IPA, and HCl concentrations, calcination temperature and coating cycle number, were optimized by Taguchi experimental design. Then, the sampling conditions, including extraction time, extraction temperature and sample volume, were optimized to get better extraction efficiency. Under optimized conditions, the method exhibited linearity over the range of 0.04–700 ppmv, with R² = 0.998–0.9995. The limits of detection for BTEX compounds were 0.015–0.142 ppmv, with acceptable repeatability (RSD &lt; 10%). The method was successfully applied for the analysis of BTEX in indoor air samples with an acceptable recovery (85–107%).</div></div>","PeriodicalId":93576,"journal":{"name":"Journal of chromatography open","volume":"9 ","pages":"Article 100320"},"PeriodicalIF":3.2,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147384618","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Magnetic extraction and preconcentration of perfluorooctanoic acid in aqueous solutions using magnetic tea waste activated carbon","authors":"Nompumelelo Malatji ,&nbsp;Anele Mpupa ,&nbsp;Boris Mizaikoff ,&nbsp;Philiswa Nosizo Nomngongo","doi":"10.1016/j.jcoa.2025.100290","DOIUrl":"10.1016/j.jcoa.2025.100290","url":null,"abstract":"<div><div>This study developed a method to determine perfluorooctanoic acid in real water samples using ultra-high performance liquid chromatography-electrospray-tandem mass spectrometry after dispersive magnetic solid phase extraction. The adsorbent used in dispersive magnetic solid phase extraction was tea waste activated carbon modified with magnetic nanoparticles. Successful synthesis of the adsorbent was confirmed by Fourier Transform infrared spectroscopy, X-ray diffraction, and energy-dispersive X-ray spectroscopy. The morphological and surface properties were investigated using scanning and transmission electron microscopy. Various experimental factors affecting the extraction and preconcentration efficiency were optimised using the design of experiments. Under the optimised conditions, the developed method had a wide linear range (39-5000 ng/L) with a correlation coefficient of 0.9915. The limit of detection was 11.7 ng/L, whereas the limit of quantification was 39.0 ng/L. The precision in terms of repeatability (intraday, n = 4 replicates) and reproducibility (interday, n = 3 days) expressed as %RSD were 2.40% and 3.10% respectively, which indicated that the developed method had good precision. The trueness of the developed method was investigated using a spike recovery test, and percentage recoveries ranged from 88.2% to 98.4 %. The magnetic activated carbon adsorbent could be reused up to 4 cycles. The developed method was successfully applied to detect perfluorooctanoic acid in three river water samples. Finally, the extent of greenness of the developed method was evaluated using six metric tools, and the findings showed that the method was environmentally friendly, cost-effective, highly practical and applicable.</div></div>","PeriodicalId":93576,"journal":{"name":"Journal of chromatography open","volume":"9 ","pages":"Article 100290"},"PeriodicalIF":3.2,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145618378","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of dispersive solid-phase extraction on multi-residue pesticide analysis in honey bees","authors":"Julia Kehmstedt ,&nbsp;Jens Pistorius ,&nbsp;Silvio Erler","doi":"10.1016/j.jcoa.2026.100343","DOIUrl":"10.1016/j.jcoa.2026.100343","url":null,"abstract":"<div><div>The QuEChERS method is one of the most popular methods to analyse pesticides in food. In recent years, it was also established in the protection of bees, as plant protection products (PPPs) are still involved in bee poisoning incidents. An additional purification step using dispersive solid-phase extraction (dSPE) is often considered necessary to obtain sufficiently pure sample extracts and increase sensitivity. Our aim was to improve the existing QuEChERS method based on honey bees, using 273 active substances and to determine differences between purification with dSPE and without.</div><div>The results show that 271 substances can be detected using either dSPE, without purification, or both. Pentachlorophenol and dichlobenil were the only substances that were not detectable using both methods. Over 70% of the active substances achieved recoveries between 70 and 120%. However, it turned out that there were more substances that did not reach the limit of acceptable recovery after purification with dSPE.</div><div>The excellent sensitivity of both methods was demonstrated by the limit of detection and quantification (LOD, LOQ), which coincided in approximately 40% of cases. For the remaining 60% of active substances, LODs and LOQs differed by one calibration point up or down for both methods. Regardless of the method, the most frequent LOQ was 10 µg/kg for LC-MS/MS and the LOQ for GC-MS(/MS) was 100 µg/kg with dSPE and 200 µg/kg without dSPE.</div><div>We can conclude that by using the improved method further purification steps are not necessarily required to reliably determine the ingredients within our analytical spectrum.</div><div>In fact, it even turned out that for substances that are sometimes toxic to bees, it is better not to further purify analytical samples. This adapted method saves time and costs in routine laboratory work and can be easily adapted to other analytes or research questions.</div></div>","PeriodicalId":93576,"journal":{"name":"Journal of chromatography open","volume":"9 ","pages":"Article 100343"},"PeriodicalIF":3.2,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147950877","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Analytical methods for in-depth characterisation of cell culture bioreactors: A case study","authors":"Janik D. Seidel ,&nbsp;Clifford Young ,&nbsp;Manuela Klingler-Hoffmann ,&nbsp;Mark R. Condina ,&nbsp;Alok Shah ,&nbsp;Sri Ramarathinam ,&nbsp;Woan Mei Kok ,&nbsp;Craig Kyngdon ,&nbsp;Peter Hoffmann","doi":"10.1016/j.jcoa.2025.100248","DOIUrl":"10.1016/j.jcoa.2025.100248","url":null,"abstract":"<div><div>Biopharmaceuticals and especially antibodies represent an expanding segment of pharmaceutical drug products. Production processes for this group of complex molecules use biological expression systems such as Chinese hamster ovary (CHO) cells, that are instrumental in their assembly, folding, and post-translational modification. Process development and optimization is challenging due to the complexity of cell culture systems and interdependency of process parameters on product quality attributes. This case study presents newly applied and developed analytical technologies able to monitor the cell culture process to increase the understanding of the interconnection of process parameters and quality attribute relationships in biopharmaceutical production processes. Specifically, a high-performance liquid chromatography (HPLC) -based workflow routinely measures amino acids and other cell culture media component profiles, while a mass spectrometry-based workflow monitors and quantifies changes in expressions of host cell proteins (HCPs) both globally and for individual HCP during the culture duration. These methods were applied to an industrial process with variations in seeding density, glucose and media feeding strategy, and media composition. Observations on product titre, global HCP and individual high-risk HCPs, throughout the culture progress, as well as in the end of the culture, provide insight for potential further optimization.</div></div>","PeriodicalId":93576,"journal":{"name":"Journal of chromatography open","volume":"8 ","pages":"Article 100248"},"PeriodicalIF":3.2,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144892109","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Selective purification of antibody-based protein scaffolds with a novel variable heavy chain 3 affinity resin","authors":"Anthony S. Ransdell,&nbsp;Melora Reed,&nbsp;John Herrington,&nbsp;Maria Hougland,&nbsp;Ronan M. Kelly","doi":"10.1016/j.jcoa.2025.100263","DOIUrl":"10.1016/j.jcoa.2025.100263","url":null,"abstract":"<div><div>The evolution of biotherapeutics to effectively treat diseases has resulted in complex molecule scaffolds compared to traditional monoclonal antibodies (mAbs). While Protein A (ProA) affinity chromatography continues to be the predominant choice for affinity-based purification of traditional mAbs, it has also been deployed for primary capture of Fc-fusions, Fabs, single variable domain on a variable heavy chain (VHHs), and heteromAbs. When binding these different molecular scaffolds, ProA offers a high-affinity interaction with the Fc-domain, or in the case of antibody-like fragments such as Fabs and VHHs, a weaker affinity interaction with certain human VH3 sequences. As a result of the dual binding mechanism of ProA, the removal of product-related impurities, such as homodimer and half-body, can be challenging when deployed in the primary purification step of a heteromAb process. To address some of the key challenges faced in the purification of these novel scaffolds, we evaluated the unique purification capabilities of the MabSelect™ VH3 affinity resin compared to MabSelect PrismA™ for the capture of VHHs, Fab-VHH fusions, mAbs, and an asymmetric antibody. Our studies showed that the purification of mAb-based scaffolds with the new MabSelect VH3 resin has similar binding capacity with comparable product quality, decreased host cell protein (HCP) content of the eluted product pool, and increased binding affinity for human VH3 sequence compared to MabSelect PrismA. Furthermore, we highlighted the advantages of utilizing this novel selective affinity resin to remove molecule-specific impurities to purify an asymmetric antibody.</div></div>","PeriodicalId":93576,"journal":{"name":"Journal of chromatography open","volume":"8 ","pages":"Article 100263"},"PeriodicalIF":3.2,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145264955","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}