DNA and RNA nanotechnology最新文献_第2页

Nucleic Acid Computing and its Potential to Transform Silicon-Based Technology 核酸计算及其改变硅基技术的潜力

DNA and RNA nanotechnology Pub Date : 2015-12-17 DOI: 10.1515/rnan-2015-0003

Seth G. Abels, Emil F Khisamutdinov

引用次数: 5

Structural identification of the novel 3 way-junction motif 新型三路结基序的结构鉴定

DNA and RNA nanotechnology Pub Date : 2015-01-18 DOI: 10.1515/rnan-2015-0004

D. Jedrzejczyk, A. Chworos

引用次数: 3

Triggering RNAi with multifunctional RNA nanoparticles and their delivery. 利用多功能 RNA 纳米粒子及其传输技术触发 RNAi。

DNA and RNA nanotechnology Pub Date : 2015-01-01 Epub Date: 2015-07-27 DOI: 10.1515/rnan-2015-0001

Bich Ngoc Dao, Mathias Viard, Angelica N Martins, Wojciech K Kasprzak, Bruce A Shapiro, Kirill A Afonin

{"title":"Triggering RNAi with multifunctional RNA nanoparticles and their delivery.","authors":"Bich Ngoc Dao, Mathias Viard, Angelica N Martins, Wojciech K Kasprzak, Bruce A Shapiro, Kirill A Afonin","doi":"10.1515/rnan-2015-0001","DOIUrl":"10.1515/rnan-2015-0001","url":null,"abstract":"Proteins are considered to be the key players in structure, function, and metabolic regulation of our bodies. The mechanisms used in conventional therapies often rely on inhibition of proteins with small molecules, but another promising method to treat disease is by targeting the corresponding mRNAs. In 1998, Craig Mellow and Andrew Fire discovered dsRNA-mediated gene silencing via RNA interference or RNAi. This discovery introduced almost unlimited possibilities for new gene silencing methods, thus opening new doors to clinical medicine. RNAi is a biological process that inhibits gene expression by targeting the mRNA. RNAi-based therapeutics have several potential advantages (i) a priori ability to target any gene, (ii) relatively simple design process, (iii) site-specificity, (iv) potency, and (v) a potentially safe and selective knockdown of the targeted cells. However, the problem lies within the formulation and delivery of RNAi therapeutics including rapid excretion, instability in the bloodstream, poor cellular uptake, and inefficient intracellular release. In an attempt to solve these issues, different types of RNAi therapeutic delivery strategies including multifunctional RNA nanoparticles are being developed. In this mini-review, we will briefly describe some of the current approaches.","PeriodicalId":93282,"journal":{"name":"DNA and RNA nanotechnology","volume":"2 1","pages":"1-12"},"PeriodicalIF":0.0,"publicationDate":"2015-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/6b/b8/nihms-1723228.PMC8315566.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39254691","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Engineered RNA Nanodesigns for Applications in RNA Nanotechnology. 用于 RNA 纳米技术应用的工程 RNA 纳米设计。

DNA and RNA nanotechnology Pub Date : 2015-01-01 Epub Date: 2013-05-31 DOI: 10.2478/rnan-2013-0001

Kirill A Afonin, Brian Lindsay, Bruce A Shapiro

引用次数: 0

Vehicles for Small Interfering RNA transfection: Exosomes versus Synthetic Nanocarriers 小干扰RNA转染载体:外泌体与合成纳米载体

DNA and RNA nanotechnology Pub Date : 2013-05-31 DOI: 10.2478/rnan-2013-0002

M. Duechler

{"title":"Vehicles for Small Interfering RNA transfection: Exosomes versus Synthetic Nanocarriers","authors":"M. Duechler","doi":"10.2478/rnan-2013-0002","DOIUrl":"https://doi.org/10.2478/rnan-2013-0002","url":null,"abstract":"Abstract Therapies based on RNA interference (RNAi) hold a great potential for targeted interference of the expression of specific genes. Small-interfering RNAs (siRNA) and micro-RNAs interrupt protein synthesis by inducing the degradation of messenger RNAs or by blocking their translation. RNAibased therapies can modulate the expression of otherwise undruggable target proteins. Full exploitation of RNAi for medical purposes depends on efficient and safe methods for delivery of small RNAs to the target cells. Tremendous effort has gone into the development of synthetic carriers to meet all requirements for efficient delivery of nucleic acids into particular tissues. Recently, exosomes unveiled their function as a natural communication system which can be utilized for the transport of small RNAs into target cells. In this review, the capabilities of exosomes as delivery vehicles for small RNAs are compared to synthetic carrier systems. The step by step requirements for efficient transfection are considered: production of the vehicle, RNA loading, protection against degradation, lack of immunogenicity, targeting possibilities, cellular uptake, cytotoxicity, RNA release into the cytoplasm and gene silencing efficiency. An exosomebased siRNA delivery system shows many advantages over conventional transfection agents, however, some crucial issues need further optimization before broad clinical application can be realized.","PeriodicalId":93282,"journal":{"name":"DNA and RNA nanotechnology","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2013-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.2478/rnan-2013-0002","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68797237","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 10

DNA Nanotechnology: From Structure to Function DNA纳米技术:从结构到功能

DNA and RNA nanotechnology Pub Date : 2013-01-01 DOI: 10.1007/978-3-642-36077-0

Chunhai Fan

引用次数: 9