International journal of molecular biology (Edmond, Okla.)最新文献

{"title":"Retrospective analysis: the research on Mycobacteria in Basilicata from 1977 to 2012","authors":"L. Buono, C. Colonna, G. Montesano","doi":"10.15406/ijmboa.2018.03.00079","DOIUrl":"https://doi.org/10.15406/ijmboa.2018.03.00079","url":null,"abstract":"The mycobacterial laboratory was devoted to the search of the Koch bacillus in the pathological materials through the microscopical examination, the examination of the culture, identification and antibiogram. The scientific progress and technical innovation have remarkably improved the mycobacterial diagnostic possibilities and consequently rapid tests of high sensibility and specificity have been developed such as the gene amplification tests used directly on the clinical samples and gene probes for the identification of non-tuberculosis mycobacteria (NTMs) which have substituted the biochemical tests of uncertain identification.","PeriodicalId":93110,"journal":{"name":"International journal of molecular biology (Edmond, Okla.)","volume":"46 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88911766","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The emergence of specific HDAC inhibitors and their clinical efficacy in the treatment of hematologic malignancies and breast cancer","authors":"David A Pride, Alyssa R Summers","doi":"10.15406/ijmboa.2018.03.00078","DOIUrl":"https://doi.org/10.15406/ijmboa.2018.03.00078","url":null,"abstract":"Epigenetic modification of gene expression through inhibition of histone deacetylases (HDAC) represents a promising potential clinical strategy for a variety of conditions, including cancer, autoimmunity and transplantation, polyglutamine disorders, neurological diseases, and heart disease. 1–4 Numerous histone deacetylase inhibitors (HDI) have been developed, showing varying degrees of specificity across classes or isotypes of HDACs. Early experience demonstrated the efficacy of less specific, “pan/global” HDIs (e.g. vorinostat, panbinostat, and abexinostat) in the treatment of specific hematological malignancies, but was tempered by significant toxicity exhibited by the inhibitor class. As the preclinical and clinical evaluation of this strategy has progressed, the field has shifted toward a focus on the development and investigation of more specific HDIs;5 hoping to limit toxicity and unintended non-target effects of HDI. Herein, we seek to review the trend in clinical research toward the development of class and isoformspecific HDIs, highlighting their clinical efficacies and limitations in the treatment of hematologic and breast cancers in order to underscore their potential to allow for the improvement of clinical outcomes with less toxicity and unintended non-target effects.","PeriodicalId":93110,"journal":{"name":"International journal of molecular biology (Edmond, Okla.)","volume":"8 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74316175","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hematology and external egg quality parameters of three Nigerian indigenous chicken genotypes","authors":"Adeyemo Go, Bo-Cheng Mo, Samuel Ehiabhi Okhale","doi":"10.15406/IJMBOA.2018.03.00077","DOIUrl":"https://doi.org/10.15406/IJMBOA.2018.03.00077","url":null,"abstract":"Information about haematological parameters and external egg quality parameters of Nigerian indigenous chickens is limited and not even available to farmers. In many species of birds especially chicken, normal values for hematological parameters were measured and a comprehensive data base was established of their blood profile. The Nigerian indigenous chicken breeds have been reported to have many advantageous gene complexes that could be harnessed in the development of meat or egg type chicken suitable for use in the tropics.1 Among these major genes are the Naked necked, Frizzled and Normal feathered. In the other hand, many researchers have evaluated normal haematological parameters of industrial and commercial hybrid chickens.2,3 Information about hematological and external egg quality parameters of indigenous chickens are limited therefore, this study was carried out to evaluate the haematological and external egg quality parameters of three strains of indigenous chickens.","PeriodicalId":93110,"journal":{"name":"International journal of molecular biology (Edmond, Okla.)","volume":"396 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-08-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76435124","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Altered protein product of PGAP2 gene disrupts cognitive pathway","authors":"Shoaib Ur Rehman, Rahmat Ali Khan","doi":"10.15406/ijmboa.2018.03.00076","DOIUrl":"https://doi.org/10.15406/ijmboa.2018.03.00076","url":null,"abstract":"","PeriodicalId":93110,"journal":{"name":"International journal of molecular biology (Edmond, Okla.)","volume":"108 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74832060","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of Peristrophe bicalyculata powder on diabetic and lipid parameters in the stomach of albino rats","authors":"Sheneni Victor Duniya, Shaibu Isaac Eleojo, Jegede Rapheal Eneji, O. Ameh, Abaniwo Rose Mafo, Mamudu Ojonugwa Collins","doi":"10.15406/ijmboa.2018.03.00075","DOIUrl":"https://doi.org/10.15406/ijmboa.2018.03.00075","url":null,"abstract":"Peristrophe bicalyculata is a herbaceous plants which belongs to the family Acanthaceae. The Hausas call it tubanin dawaki in Nigeria, which means horse flour. In Senegal the serer and Wolof language call it buben and moto.1 It is called chotiharjori2 by the Indore zone of the Indians. Naturally it is found in the warm tropical areas of Africa, in the sahel area of Mauritania, northern part of Nigeria and also in Thailand, India and Burma. The plant is used locally in the treatment of diabetes in the south western part of Nigeria. The plant is a stimulant, stomachic and a diuretic. Chewing the plant leaves suppresses the sweet and bitter in it. The consumers of this usually expects a reducing effect on weight and betterment of diabetes due to its ability to reduce taste of sweet and prevent the absorption of glucose by the cells. Diabetes mellitus is a metabolic disorder characterized by elevated blood sugar level, abnormalities in lipoprotein metabolism and intermediary embolism of major food substances.3 Rare cases of hepatic inflammation induced by anthraquinone have been documented,4 and are dose depended. Anthrax quinines are metabolised in the small intestines, forming hepatic toxic substances that some people are sensitive to. This substances result to reversible liver abnormality. Gastrointestinal disorder is one of the prevailing reasons why people seek medical advice. About approximately 15– 30% of full grown adults suffer from this condition. The discovery of functional drugs is one of the major areas of phototherapeutic treatment today. From the day immemorial, bitter herbal drugs from neem or vernonia amyygdalina has been implicated in the treatment of patients with dyspeptic complaint. The mechanism of action of how this bitter herb elicits their biological function is not fully understood. But it is believed stimulate the stomach secretion, digestive glands and strengthens gastrointestinal tract thus stimulating the CNS, leading to notification. Bitters, especially at a very high dose affect positively the mucous membrane lining of the stomach and bowel. They are usually combined with essential oil, which are known for their spasmolytic, aesthetic and carminative effect.5 This study shows the effect of various concentrations of Peristrophe bicalyculata in albino rats. Methodology","PeriodicalId":93110,"journal":{"name":"International journal of molecular biology (Edmond, Okla.)","volume":"44 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-07-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86068730","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Metabolic regulation in innate immunity","authors":"Samin Ayromlou, L. Hatamnezhad, H. Ayromlou, S. Siamak, oghchian Shotorbani","doi":"10.15406/ijmboa.2018.03.00074","DOIUrl":"https://doi.org/10.15406/ijmboa.2018.03.00074","url":null,"abstract":"Abbreviations: CNS, central nervous system; G6P, glucose 6-phosphate; GluT, glucose transferase; ATP, Adenosine three phosphate; NADPH, nicotinamide adenine dinucleotide phosphate; MAPKS, mitogen activated protein kinases; ROS/RNS, reactive oxygen/reactive nitrogen species; G6PD, glucose-6-phosphate dehydrogenase; NF-κB, nuclear factor-κB nox: NADPH Oxidase 2; MIF, migration inhibitory factor; PKC, protein kinase C; TLR, tolllike receptors; PRRS, pattern recognition receptor; Myd88, myeloid differentiation primary response gene 88; TIR, toll/Interleukin-1 receptor; IRAK4, IL-1 receptor-associated kinase 4; TRAF6, TNF receptor associated factor; AP-1, activator protein 1 IKK: IκB kinase; JNK, c-Jun N-terminal kinases; TCR, T-cell receptor; TNF-α, tumor necrosis factor-α; Il-6, Interleukin-6; LIF, leukemia inhibitory factor; Il-1ß, Interleukin-1ß Il-1; Acp, The IL-1 accessory protein; Gp130, Glycoprotein 130; JAK, Janus kinase; STAT, signal transducer and activator of transcription; CRP, C-reactive protein.","PeriodicalId":93110,"journal":{"name":"International journal of molecular biology (Edmond, Okla.)","volume":"111 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-07-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79192187","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enzyme kinetics of RNase present in testes","authors":"Eswari Beeram, Divya Bysani, C. Pallavi, K. Thyagaraju","doi":"10.15406/ijmboa.2018.03.00073","DOIUrl":"https://doi.org/10.15406/ijmboa.2018.03.00073","url":null,"abstract":"Enzyme kinetics is one of the important parameter to be studied to know the inhibition studies of RNase A. RNase A is an allosteric enzyme which consists of an allosteric site in addition to the active site of the enzyme. With respect to allosteric enzymes two types of modulators namely positive and negative regulates the enzyme. Positive allosteric modulators increase the cooperitivity to other sites in positive manner for a multi subunit protein. So, K0.5 is usually decreased and Vmax found to be increased.1 Where as negative modulators decrease the affinity of substrate binding at one subunit of enzyme compared to other subunits of it. So, K0.5 found to be increased and Vmax decreases.1 O2 binding to the Hb is an example of positive cooperivity and feedback inhibition by allosteric enzyme is an example of negative cooperitivity. UV visible spectroscopic analysis in vitro proved RNase A is an allosteric enzyme and agarose gel electrophoresis analysis2 has proved that metosartan is an inhibitor of RNase A. Various plots of enzyme like Michaelis–Menton plot, Line weaverburk plot, Dixon plot and Eadiehofstee plot are used to know the K0.5 and Vmax of the enzyme in the presence and absence of drug. As RNase A is an allosteric enzyme it doesn’t follows Michaelsmenton kinetics as it consists of more than one active site. Allosteric enzymes give profound sigmoid curve with negative modulator and hyperbolic curve with positive modulator. Line weaver – Burk plot is not useful in case of allosteric enzymes to know whether the inhibition pattern is allosteric or not. Dixon plots are used to know the potency of inhibitor over the enzyme and Eadie – Hofstee plot is useful to know the Km/Vmax and Km. But it is difficult to know the Km and Vmax of the enzymes by EadieHofstee plot. Enzyme kinetics for each concentration is plotted and its effect of drug on enzyme was studied. The term Km is not used for allosteric enzymes instead K0.5 is used as the enzyme doesn’t follows Michaels menton kinetics. Properties of allosteric enzymes are as follows. Higher substrate concentration favours R state of the enzyme where as lower concentration favours T state where as other molecules like O2 and 2,3-Bis phosphor glycerate are regulators of allosteric enzymes.","PeriodicalId":93110,"journal":{"name":"International journal of molecular biology (Edmond, Okla.)","volume":"27 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-07-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76159312","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Invasive alien plant species in Ethiopia: ecological impacts on biodiversity a review paper","authors":"Wakshum Shiferaw, S. Demissew, T. Bekele","doi":"10.15406/IJMBOA.2018.03.00072","DOIUrl":"https://doi.org/10.15406/IJMBOA.2018.03.00072","url":null,"abstract":"Ethiopia has great geographic diversity, and macro and micro -climatic variability. However, there are threats its biodiversity by habitat conversion, invasive species, unsustainable utilization of biodiversity resources, replacement of local varieties and breeds, climate change and pollution, demographic change, poverty, and lack of awareness and coordination.1 Invasive species are either indigenous or non indigenous species that can heavily colonize a particular habitat.2 Alien species are non-native or exotic organisms that occur outside their adaptive ranges and dispersal ranges.3,4 IAS are all categories of living organisms (plants, mammals and insects) which comprise the most common types in terrestrial environments.4 They are also widely distributed in all kinds of ecosystems throughout the world. IAS have the ability to establish themselves, invade, outcompete natives and take over the new environment.3,5 They are now affecting every ecosystem types on the planet and they are the second greatest global threat to biodiversity after habitat destruction.4,6 They are pausing threats to ecosystems of the earth, and the services that they provide to humanity are under risk.5 IAS have induce impacts on native species directly competing for resource such as: food and breeding sites indirectly by altering habitat and modify hydrology, nutrient cycling and other ecosystem processes. Their impacts are on native biodiversity in ecosystems like agricultural and range lands, national parks, water ways, lakes, rivers, power dams, road sides and urban green spaces with huge economical as well as social consequences. These dramatically change the ecosystem both positively and negatively. Across impact types, the positive impacts reported by most authors were found to be largely outweighed by negative impacts.7–9","PeriodicalId":93110,"journal":{"name":"International journal of molecular biology (Edmond, Okla.)","volume":"10 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-07-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88575852","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of preservative on foetus tissues and DNA profiling in forensic cases","authors":"Naresh Kumar, Aanchal Maitray, Ritika Gupta, S. Shukla","doi":"10.15406/ijmboa.2018.03.00071","DOIUrl":"https://doi.org/10.15406/ijmboa.2018.03.00071","url":null,"abstract":"It was in the news when a 10 year old girl became pregnant at Chandigarh in India after being repeatedly raped by a relative in 2017. There are lots of cases of teenage pregnancies involving 14 to 17 year olds. The girl in question has been described as a happy smiley child. On 28 July, Supreme Court of India rejected a petition filed on her behalf to allow her to abort, on the grounds that it is 32 weeks pregnancy. A doctors’ panel was formed and advised the court that a termination at this stage would be too risky for the girl, and foetus was doing well. It was a huge disappointment for the girl’s family. In another case a girl eloped with some friend on 6 September at 7 AM in the morning when she went to the school and did not turn back. First Information report was registered in the nearby police station. The girl was recovered on 31 March and medical examination was done and she was declared pregnant. MTP was done on the 12 May. Total five items were collected including part of foetus in the form of tissue in normal Saline (NS), complete foetus in formalin and placenta in two parts in formalin as well as normal saline, along with blood sample of the victim.","PeriodicalId":93110,"journal":{"name":"International journal of molecular biology (Edmond, Okla.)","volume":"30 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87973530","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biodegradation of pesticide complex chlorpyrifos + cypermethrin by microorganisms isolated from the Southern Aral Sea region","authors":"Tashpulatov Jj, Zaynitdinova Li, Juraeva Rn, Lobanova, Kukanova Si","doi":"10.15406/ijmboa.2018.03.00070","DOIUrl":"https://doi.org/10.15406/ijmboa.2018.03.00070","url":null,"abstract":"Anthropogenic natural disaster of the Aral Sea and decreasing outflow of rivers Amudarya and Syrdarya caused a number of irreversible changes in soil microbial communities in the Southern Aral Sea region. Common features of these soils is their high salinization, extreme instability of humidity level up to total draught, and high content of different xenobiotics.1,2 Soil borne microorganisms are one of the main factors controlling degradation of xenobiotics in the natural ecosystem and essential bioindicators reflecting changes in soil, which is related to their enormous importance in soil formatting processes. There is possibility of emergence of strains in microbial populations of such soils that are capable to active degradation of pesticides.3,4 Such cultures, undoubtedly, are perspective for rehabilitation of natural environments.5 The approach engaging microorganismsbiodestructors for rehabilitation of soils polluted with agrochemicals has certain efficiency and effectiveness. Indigenous strains of soil borne microorganisms possessing both ability to destroy pesticides and natural resistance to the complex of pollutants and sharp changes of climatic conditions stipulate importance and feasibility of this approach. In these regards, the microbial biota of the Southern Aral Sea region, characterized by extreme natural conditions, was studied. Different microorganisms were isolated and their ability to destroy pesticides was determined.","PeriodicalId":93110,"journal":{"name":"International journal of molecular biology (Edmond, Okla.)","volume":"101 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89926556","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}