Basic and applied pathology最新文献_第7页

ORIGINAL ARTICLE: Diagnostic immunomarkers for HepPar-1 negative areas of hepatocellular carcinoma 原文章:肝细胞癌HepPar-1阴性区域的诊断性免疫标志物

Basic and applied pathology Pub Date : 2010-09-06 DOI: 10.1111/j.1755-9294.2010.01074.x

Tae-Hui Lee, Hyunee Yim, Jae Yeon Seok, Young-Bae Kim

{"title":"ORIGINAL ARTICLE: Diagnostic immunomarkers for HepPar-1 negative areas of hepatocellular carcinoma","authors":"Tae-Hui Lee, Hyunee Yim, Jae Yeon Seok, Young-Bae Kim","doi":"10.1111/j.1755-9294.2010.01074.x","DOIUrl":"10.1111/j.1755-9294.2010.01074.x","url":null,"abstract":"<div>\u0000 \u0000 <p> <b>Background and aim:</b> HepPar-1 is a sensitive and specific antibody. However, when used for the diagnosis of hepatocellular carcinoma (HCC), especially in needle biopsy specimens, this diagnostic approach is limited by potential multifocal expression and/or poorly differentiated HCCs, where it may not be expressed. The goal of this study was to determine the antibodies most sensitive to hepatocyte differentiation in HepPar-1 negative areas including: CD13, CD10, polyclonal carcinoembryonic antigen (pCEA), alpha-fetoprotein (AFP) and glypican-3, for needle biopsy. <b>Methods:</b> Fifty cases of HCCs were obtained from surgically resected livers. Immunohistochemical staining for hepatocyte and biliary markers- HepPar-1, CD13, glypican-3, pCEA, CD10, AFP, cytokeratin (CK)7, CK19, and epithelial cell adhesion molecule (EpCAM) was performed from paraffin block samples. <b>Results:</b> HepPar-1 was diffusely negative in 12 cases. Twenty two cases showed partial expression of the HepPar-1 antibody. Among 34 HepPar-1 negative areas, CD13 was the most consistent antibody (85.3%, 29/34) showing the characteristic bile canaliculi pattern. Immunoexpression of biliary differentiation with CK7, CK19, and EpCAM showed no significant differences in HepPar-1 negative areas. <b>Conclusions:</b> CD13 could be used for the detection of hepatocyte differentiation in HCCs with HepPar-1 negative expression, especially in needle biopsy specimens. In addition, negative immunoexpression for HepPar-1 was not overtly related to biliary differentiation, from an immunohistochemical viewpoint.</p>\u0000 </div>","PeriodicalId":92990,"journal":{"name":"Basic and applied pathology","volume":"3 3","pages":"80-85"},"PeriodicalIF":0.0,"publicationDate":"2010-09-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/j.1755-9294.2010.01074.x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"63681383","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

ORIGINAL ARTICLE: Immunohistochemical identification of basal-like breast carcinoma and Its histopathologic correlation 原文章:基底样乳腺癌的免疫组织化学鉴定及其组织病理学相关性

Basic and applied pathology Pub Date : 2010-09-06 DOI: 10.1111/j.1755-9294.2010.01072.x

Susie Chin, Eun Suk Koh, Jeong Ja Kwak, Hee Kyung Kim, Hee Jung Lee, Cheol Wan Lim

{"title":"ORIGINAL ARTICLE: Immunohistochemical identification of basal-like breast carcinoma and Its histopathologic correlation","authors":"Susie Chin, Eun Suk Koh, Jeong Ja Kwak, Hee Kyung Kim, Hee Jung Lee, Cheol Wan Lim","doi":"10.1111/j.1755-9294.2010.01072.x","DOIUrl":"10.1111/j.1755-9294.2010.01072.x","url":null,"abstract":"<div>\u0000 \u0000 <p> <b>Background and aim:</b> DNA expression profiling studies of breast cancer have identified subtypes including luminal A, luminal B, human epidermal growth factor receptor 2 (HER2) overexpression, and basal-like type. Among them, the basal-like type was associated with poor outcome. The purpose of this study was to classify breast carcinoma by immunohistochemistry and evaluate its characteristic histopathologic features. <b>Methods:</b> One hundred and four cases of resected infiltrating ductal carcinoma were immunostained for estrogen receptor, HER2, cytokeratin 5/6 and p63 to classification, and basal-like subtype were evaluated for its histopathologic characteristics. <b>Results:</b> The prevalence of subtypes was luminal A 48.1%, luminal B 24.0%, HER2 overexpression 11.5%, normal breast-like 4.8%, and basal-like 11.5%. There was significant correlation between the basal-like type and histologic grade. And it was significantly associated with several morphological features including expanding growth pattern, presence of tumor necrosis and peritumoral lymphocytic infiltration. <b>Conclusions:</b> The results of this study showed that the basal-like type occupied 11.5% of infiltrating ductal carcinoma and was associated with expanding growth pattern, presence of tumor necrosis and peritumoral lymphocytic infiltration.</p>\u0000 </div>","PeriodicalId":92990,"journal":{"name":"Basic and applied pathology","volume":"3 3","pages":"86-92"},"PeriodicalIF":0.0,"publicationDate":"2010-09-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/j.1755-9294.2010.01072.x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"63681575","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

ORIGINAL ARTICLE: Expression pattern of glypican-3, HSP70, and glutamine synthetase in chronic hepatitis B and hepatocellular carcinoma glypican-3、HSP70和谷氨酰胺合成酶在慢性乙型肝炎和肝细胞癌中的表达模式

Basic and applied pathology Pub Date : 2010-09-06 DOI: 10.1111/j.1755-9294.2010.01083.x

Soo Hee Kim, Kyoung Bun Lee, Hyeong-Ju Kwon, Ja-June Jang

{"title":"ORIGINAL ARTICLE: Expression pattern of glypican-3, HSP70, and glutamine synthetase in chronic hepatitis B and hepatocellular carcinoma","authors":"Soo Hee Kim, Kyoung Bun Lee, Hyeong-Ju Kwon, Ja-June Jang","doi":"10.1111/j.1755-9294.2010.01083.x","DOIUrl":"10.1111/j.1755-9294.2010.01083.x","url":null,"abstract":"<div>\u0000 \u0000 <p> <b>Background and aim:</b> The three-panel marker, glypican-3, HSP70 and glutamine synthetase, is helpful for making a diagnosis of early hepatocellular carcinoma. To further characterize the diagnostic values of these three markers, we performed immunostaining studies in non-neoplastic hepatocytes (chronic hepatitis B) and hepatocellular carcinoma. <b>Methods:</b> Immunostaining was performed for all three markers. The cases were 60 needle biopsies of chronic hepatitis B (30 with low-grade activity and 30 with high-grade activity) and 40 hepatocellular carcinomas (HCCs). <b>Results:</b> Glypican-3 staining was detected in only one of the 60 chronic hepatitis B cases, and this case expressed moderate immunoreactivity for glypican-3 and showed high-grade inflammatory activity. HSP70 expression was not observed in any cases. Glutamine synthetase was strongly immunoreactive at peri-venular hepatocytes with cytoplasmic staining in all cases. These findings reflected a normal expression pattern. On the other hand, all three markers were positive in 15 (37.5%) HCCs. At least two of three markers were positive in 26 (65.0%). Moreover, all three markers were negative in 2 (5.0%) HCCs. <b>Conclusions:</b> The combination of these three markers is highly specific for detecting hepatocellular carcinoma. However, caution should be exercised because non-neoplastic hepatocytes in chronic hepatitis B with high necro-inflammatory activity tend to show focal positivity for glypican-3. Also we emphasize that all three markers can show negative immunoreactivity in HCC.</p>\u0000 </div>","PeriodicalId":92990,"journal":{"name":"Basic and applied pathology","volume":"3 3","pages":"75-79"},"PeriodicalIF":0.0,"publicationDate":"2010-09-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/j.1755-9294.2010.01083.x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"63682233","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

CASE REPORT: Extralobar pulmonary sequestration presenting a paravertebral mass with extensive fibroadenomatous change in an adult 病例报告:一例成人椎外肺隔离表现为椎旁肿块伴广泛纤维腺瘤改变

Basic and applied pathology Pub Date : 2010-06-14 DOI: 10.1111/j.1755-9294.2010.01076.x

Song Yi Choi, Jungsuk An, Jae Jun Lee, Joungho Han, Young Mog Shim, Chin A. Yi

引用次数: 0

ORIGINAL ARTICLE: Morphometric evaluation for relationship of atherosclerosis developed in coronary and peripheral muscular arteries: An autopsy study 原文章:冠状动脉和外周肌肉动脉粥样硬化关系的形态学评估:一项尸检研究

Basic and applied pathology Pub Date : 2010-06-14 DOI: 10.1111/j.1755-9294.2010.01080.x

Seong Muk Jeong, Jung Hee Lee, Won Young Park, Chang Hun Lee, Do Youn Park, Kwang Hoon Kim, Gam Rae Jo, Sang Yong Lee, Gi Yeong Huh

{"title":"ORIGINAL ARTICLE: Morphometric evaluation for relationship of atherosclerosis developed in coronary and peripheral muscular arteries: An autopsy study","authors":"Seong Muk Jeong, Jung Hee Lee, Won Young Park, Chang Hun Lee, Do Youn Park, Kwang Hoon Kim, Gam Rae Jo, Sang Yong Lee, Gi Yeong Huh","doi":"10.1111/j.1755-9294.2010.01080.x","DOIUrl":"10.1111/j.1755-9294.2010.01080.x","url":null,"abstract":"<div>\u0000 \u0000 <p> <b>Background and aims:</b> Atherosclerosis is a systemic disorder that develops in any blood vessel of the body. Atherosclerosis can result in fatal complications such as myocardial infarction or stroke. The aim of this study was to compare the severity of atherosclerosis between the coronary arteries and other peripheral arteries. To our knowledge, no such morphometric tissue studies through autopsy samples so far are available in Korea. <b>Methods:</b> The left anterior descending artery, right coronary artery and bilateral carotid, renal and femoral arteries were obtained from 50 autopsies during the period from June to October 2008. Detailed gross examination of the each arteries was performed to look for the most severe site of luminal narrowing. <b>Results:</b> There was a significant correlation between the maximal carotid artery intima-media thickness and the largest plaque area of the coronary artery (<i>P</i> < 0.05). However, some discordant cases were also noted. Seven cases had more severe coronary atherosclerosis compared to the carotid artery. <b>Conclusions:</b> The careful examination of peripheral arteries including the carotid artery can be helpful in clinical prediction of coronary artery disease. However, such prediction should include other risk factors such as age, hypertension, diabetes and dyslipidemia and other relevant clinical conditions.</p>\u0000 </div>","PeriodicalId":92990,"journal":{"name":"Basic and applied pathology","volume":"3 3","pages":"93-97"},"PeriodicalIF":0.0,"publicationDate":"2010-06-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/j.1755-9294.2010.01080.x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"63682162","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

CASE REPORT: Epithelial-myoepithelial carcinoma of the bronchus 病例报告:支气管上皮-肌上皮癌

Basic and applied pathology Pub Date : 2010-06-14 DOI: 10.1111/j.1755-9294.2010.01077.x

Young Jin Jun, Se Min Jang, Woong Na, Ki-Seok Jang, Won Sang Chung, Seung Sam Paik

{"title":"CASE REPORT: Epithelial-myoepithelial carcinoma of the bronchus","authors":"Young Jin Jun, Se Min Jang, Woong Na, Ki-Seok Jang, Won Sang Chung, Seung Sam Paik","doi":"10.1111/j.1755-9294.2010.01077.x","DOIUrl":"10.1111/j.1755-9294.2010.01077.x","url":null,"abstract":"<div>\u0000 \u0000 <p>Pulmonary epithelial-myoepithelial carcinoma is a rare low-grade malignant neoplasm that has the characteristic histologic features, epithelial and myoepithelial differentiation, similar to the salivary gland counterpart. This tumor is thought to arise from bronchial submucosal glands distributed throughout the lower respiratory tract. We recently experienced a case of epithelial-myoepithelial carcinoma of the bronchus in a 61-year-old man. Computed tomography of his chest revealed an endobronchial mass in the bronchus of the left lower lobe. The lobectomy specimen showed a solitary endobronchial mass, measuring 2.3 cm in greatest diameter. Histologically, the tumor was composed of variable proportions of epithelial and myoepithelial cells. The epithelial cells represented an inner layer of duct-like structures and the outer clear cell layer was myoepithelial-type cells. The epithelial cells were positive for pancytokeratin and the clear myoepithelial cells were positive for vimentin, alpha-smooth muscle actin and S-100 protein. Finally our diagnosis was epithelial-myoepithelial carcinoma of the bronchus.</p>\u0000 </div>","PeriodicalId":92990,"journal":{"name":"Basic and applied pathology","volume":"3 3","pages":"101-104"},"PeriodicalIF":0.0,"publicationDate":"2010-06-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/j.1755-9294.2010.01077.x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"63681876","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

West-Libyan propolis and rosemary have synergistic anti-tumor effect against 12-O-tetradecanoylphorbol 13-acetate-induced skin tumor in BULB/C mice previously initiated with 7,12-dimethylbenz[a]anthracene 西利比亚蜂胶和迷迭香对先前用7,12-二甲基苯[a]蒽引发的12- o -十四烷酚13-醋酸酯诱导的BULB/C小鼠皮肤肿瘤具有协同抗肿瘤作用

Basic and applied pathology Pub Date : 2010-06-02 DOI: 10.1111/j.1755-9294.2010.01079.x

Sahar S Abd El-Rahman

{"title":"West-Libyan propolis and rosemary have synergistic anti-tumor effect against 12-O-tetradecanoylphorbol 13-acetate-induced skin tumor in BULB/C mice previously initiated with 7,12-dimethylbenz[a]anthracene","authors":"Sahar S Abd El-Rahman","doi":"10.1111/j.1755-9294.2010.01079.x","DOIUrl":"10.1111/j.1755-9294.2010.01079.x","url":null,"abstract":"<div>\u0000 \u0000 <p> <b>Background and aim:</b> Many dietary constituents are chemopreventive in animal models and experiments with cultured cells are revealing various potential mechanisms of action. Many of these compounds can prevent, or greatly reduce, initiation of carcinogenesis, or can act on cell proliferation. In this study we examined the effects of topical application of propolis extract, rosemary extract and a mixture of both extracts together on 12-O-tetradecanoylphorbol 13-acetate-induced tumor promotion in mice previously initiated with 7,12-dimethylbenz[a]anthracene. <b>Methods:</b> The propolis extract, rosemary extract and a mixture of both extracts together were applied topically 15 min prior to the application of 5 nmol TPA for 20 weeks. <b>Results:</b> A decrease in the number of skin tumors per mouse by 27, 39, 71% was seen, as well as a decrease in the number of mice with tumors by 22, 31, 75% and inhibited the tumor size per mouse by 46, 62, 72% for propolis, rosemary and both together respectively. Also TUNEL data showed more increased incidence of TUNEL positivity among cancer cells in both topical applications together than their separate application on the back skin of mice. <b>Conclusions:</b> It is likely that the combination of their activities such as antioxidant and cytotoxic ones as well as the combination of several components in both are responsible for their inhibitory effect on carcinogenesis. Further investigations are recommended in order to establish the conditions under which topical application of propolis as well as rosemary produces either protective or deleterious effects.</p>\u0000 </div>","PeriodicalId":92990,"journal":{"name":"Basic and applied pathology","volume":"3 2","pages":"46-51"},"PeriodicalIF":0.0,"publicationDate":"2010-06-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/j.1755-9294.2010.01079.x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"63682054","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

Comparison of the diagnostic methods on the canine adenovirus type 2 infection 犬腺病毒2型感染诊断方法的比较

Basic and applied pathology Pub Date : 2010-06-02 DOI: 10.1111/j.1755-9294.2010.01073.x

Soon-Seek Yoon, Jae-Won Byun, Young-Il Park, Min-Jeong Kim, You-Chan Bae, Jae-Young Song

{"title":"Comparison of the diagnostic methods on the canine adenovirus type 2 infection","authors":"Soon-Seek Yoon, Jae-Won Byun, Young-Il Park, Min-Jeong Kim, You-Chan Bae, Jae-Young Song","doi":"10.1111/j.1755-9294.2010.01073.x","DOIUrl":"10.1111/j.1755-9294.2010.01073.x","url":null,"abstract":"<p> <b>Background and aims:</b> Canine adenovirus type 2 (CAV-2) infection is typically diagnosed histopathologically since intranuclear inclusion bodies (IN/IBs) are demonstrable in the infected lung. However, it is sometimes difficult to identify IN/IBs, particularly in autolyzed tissues or samples from both early and late stages of infection, and other methods were presently developed. <b>Methods:</b> Stray dog samples were evaluated by histopathology, polymerase chain reaction (PCR), and immunohistochemistry (IHC) to investigate the status of the CAV-2 infection on the stray dogs in Korea. Histologic tests were performed, and dogs with pneumonic lungs were further evaluated by IHC and PCR. <b>Results:</b> Pathognomonic IN/IBs were identified in 3 of 213 lungs; CAV-2 PCR was positive for 27 of 213 pneumonic lungs. A total of 7 of 27 CAV-2 PCR-positive lungs were IHC-positive. No PCR-negative lung was IHC-positive. Positive results were primarily detected in the IN/IBs of the bronchial epithelial cells, macrophages, and very rarely in the cytoplasm of bronchial epithelial cells. <b>Conclusions:</b> IHC was a more reliable diagnostic method than conventional pathologic methods in the present study, and suggests that IHC should be routinely used in the diagnosis of CAV-2 infection. Further, PCR alone may not be adequate for CAV-2 diagnosis.</p>","PeriodicalId":92990,"journal":{"name":"Basic and applied pathology","volume":"3 2","pages":"52-56"},"PeriodicalIF":0.0,"publicationDate":"2010-06-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/j.1755-9294.2010.01073.x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"37895888","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 13

Primary retroperitoneal meningioma: A case report 原发性腹膜后脑膜瘤1例

Basic and applied pathology Pub Date : 2010-06-02 DOI: 10.1111/j.1755-9294.2009.01066.x

So Young Lee, Ji Hun Kim, Mi Jung Kim, Kyung Ja Cho, Eun Sil You, Shin Kwang Khang

{"title":"Primary retroperitoneal meningioma: A case report","authors":"So Young Lee, Ji Hun Kim, Mi Jung Kim, Kyung Ja Cho, Eun Sil You, Shin Kwang Khang","doi":"10.1111/j.1755-9294.2009.01066.x","DOIUrl":"10.1111/j.1755-9294.2009.01066.x","url":null,"abstract":"<div>\u0000 \u0000 <p>Primary extracranial meningiomas are rare disease entities, occurring primarily in the head and neck. We herein present a very rare case of primary extracranial meningioma of the retroperitoneum, observed in a 56-year-old man who presented with a retroperitoneal mass measuring 11 cm in its greatest dimension. Histological examination revealed sheets of spindle cells with frequent whorling patterns. The neoplastic cells had abundant light eosinophilic cytoplasm with minimal mitosis or cellular atypism. Immunohistochemical analysis demonstrated diffuse and strong positivity for vimentin and epithelial membrane antigen. Ultrastructural findings revealed abundant intermediate filaments, complex interdigitating cellular processes, and desmosome-like intercellular junctions, all of which are characteristic ultrastructural features of meningiomas. The patient was free of recurrence or metastasis 11 months after surgery. To the best of our knowledge, this is only the second reported case of primary retroperitoneal meningioma.</p>\u0000 </div>","PeriodicalId":92990,"journal":{"name":"Basic and applied pathology","volume":"3 2","pages":"63-66"},"PeriodicalIF":0.0,"publicationDate":"2010-06-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/j.1755-9294.2009.01066.x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"63679864","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

From the Editor 来自编辑器

Basic and applied pathology Pub Date : 2010-06-02 DOI: 10.1111/j.1755-9294.2010.01082.x

Jeong-Wook Seo M.D. , Michele Hernandez Diwa M.D.

引用次数: 0