Basic and applied pathology最新文献_第4页

Gastric mucin and beta-catenin are useful markers to predict submucosal invasion and lymph node metastasis in intestinal type early gastric cancer 胃粘蛋白和β -连环蛋白是预测肠型早期胃癌粘膜下浸润和淋巴结转移的有用标志物

Basic and applied pathology Pub Date : 2011-08-08 DOI: 10.1111/j.1755-9294.2011.01110.x

Woo-Gyeong Kim, Nari Shin, Min-Gyeong Park, Kyung-Bin Kim, Ahrong Kim, Joo-Yeon Kim, Dong Hoon Shin, Kyung-Un Choi, Jee-Yeon Kim, Do Youn Park

{"title":"Gastric mucin and beta-catenin are useful markers to predict submucosal invasion and lymph node metastasis in intestinal type early gastric cancer","authors":"Woo-Gyeong Kim, Nari Shin, Min-Gyeong Park, Kyung-Bin Kim, Ahrong Kim, Joo-Yeon Kim, Dong Hoon Shin, Kyung-Un Choi, Jee-Yeon Kim, Do Youn Park","doi":"10.1111/j.1755-9294.2011.01110.x","DOIUrl":"10.1111/j.1755-9294.2011.01110.x","url":null,"abstract":"<div>\u0000 \u0000 Background and aim: With the increasing therapeutic use of endoscopic resection for intestinal early gastric cancer (EGC), it is very important to predict biological behaviors of intestinal type EGC prior to endoscopic resection. On this background, we tried to elucidate the presumptive clinicopathologic factors and biologic markers to predict submucosal invasion and lymph node metastasis based on mucin expression in EGCs. Methods: One hundred and thirty cases of intestinal EGCs were divided as EGCs with intestinal mucin phenotypes (EGC-IPs) and EGCs with gastric mucin phenotypes (EGC-GPs) based on mucin expression. The expressions of mucins (Muc2, Muc5Ac, Muc6, CD10), other protein markers (p53, CDX2, beta-catenin, E-cadherin, Smad4) by immunohistochemistry were studied. Results: EGC-IPs showed significantly increased p53 expression, CDX2 expression and beta-catenin delocalization than EGC-GPs. Using binary logistic regression analysis, expression of both gastric mucin and nuclear beta-catenin expression could be independent predictive factors of lymph node metastasis and submucosal invasion in intestinal EGCs. Lymphovascular emboli, and size of lesion could be clinicopathological independent predictive factors of lymph node metastasis and submucosal invasion in intestinal EGCs, respectively. Conclusions: Taken together, we suggest that beta-catenin expression based on mucin phenotype might be used to predict biologic behavior prior to endoscopic mucosal resection in intestinal EGC.\u0000 </div>","PeriodicalId":92990,"journal":{"name":"Basic and applied pathology","volume":"4 3","pages":"86-92"},"PeriodicalIF":0.0,"publicationDate":"2011-08-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/j.1755-9294.2011.01110.x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"63684637","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Primary meningeal melanocytoma of cerebellopontine angle: A case report with 12 years follow up 脑桥小脑角原发性脑膜黑素细胞瘤1例，随访12年

Basic and applied pathology Pub Date : 2011-07-27 DOI: 10.1111/j.1755-9294.2011.01109.x

Sushila Jaiswal, Awadhesh Kumar Jaiswal, Mukul Vij, Sanjay Behari, Rakesh Pandey

引用次数: 2

Frequent activation of mammalian target of rapamycin in primary lung adenocarcinoma and preinvasive lesion 哺乳动物雷帕霉素靶蛋白在原发性肺腺癌和侵袭前病变中的频繁激活

Basic and applied pathology Pub Date : 2011-07-12 DOI: 10.1111/j.1755-9294.2011.01108.x

Younghye Kim, Hoiseon Jeong, Aeree Kim, Han Kyeom Kim, Bong Kyung Shin

{"title":"Frequent activation of mammalian target of rapamycin in primary lung adenocarcinoma and preinvasive lesion","authors":"Younghye Kim, Hoiseon Jeong, Aeree Kim, Han Kyeom Kim, Bong Kyung Shin","doi":"10.1111/j.1755-9294.2011.01108.x","DOIUrl":"10.1111/j.1755-9294.2011.01108.x","url":null,"abstract":"<div>\u0000 \u0000 Background and aim: The mammalian target of rapamycin (mTOR) pathway is important for cell survival, growth and proliferation and may be involved in the histological progression of lung adenocarcinoma (AC). Methods: We compared the expression of the mTOR signal pathway molecules, i.e., phosphorylated m-TOR (p-mTOR), phosphatase and tensin homolog deleted on chromosome ten (PTEN), epidermal growth factor receptor (EGFR), and cyclin D1, among lung AC, adenocarcinoma in situ (AIS) including both pure bronchioloalveolar carcinoma (BAC) and peripheral BAC-like lesions around invasive carcinoma, atypical adenomatous hyperplasia (AAH), and normal parenchyma surrounding tumor by immunohistochemistry. The protein expression was also correlated with patients’ clinicopathological data on smoking history, tumor stage at diagnosis, recurrence and survival. Results: Intermediate or strong immunoreactivity for p-mTOR was seen in 84.1% of AC and 90.2% of AIS. Partial or complete loss of PTEN was demonstrated in 88.8% of AC and 30.4% of AIS. A significant trend of increasing EGFR, loss of PTEN, p53 expression and Ki-67 labeling index were observed by histological progression from AAH to invasive AC through AIS. Expression of p-mTOR was associated with smoking status. Conclusions: Activation of mTOR can occur by various stimuli and may be an early event in the carcinogenesis of lung AC, and may be associated with sex and smoking status of the patient.\u0000 </div>","PeriodicalId":92990,"journal":{"name":"Basic and applied pathology","volume":"4 3","pages":"78-85"},"PeriodicalIF":0.0,"publicationDate":"2011-07-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/j.1755-9294.2011.01108.x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"63684776","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Histopathological alterations in organ structures of hypercholesterolemic rats fed Ortanique peel polymethoxylated flavones 喂枸杞皮多甲氧基黄酮对高胆固醇血症大鼠器官结构的组织病理学改变

Basic and applied pathology Pub Date : 2011-07-12 DOI: 10.1111/j.1755-9294.2011.01107.x

Curtis O. Green, Andrew O. Wheatley, Barrie Hanchard, Tracey N. Gibson, Donovan A. McGrowder, Lowell L. Dilworth, Helen N. Asemota

{"title":"Histopathological alterations in organ structures of hypercholesterolemic rats fed Ortanique peel polymethoxylated flavones","authors":"Curtis O. Green, Andrew O. Wheatley, Barrie Hanchard, Tracey N. Gibson, Donovan A. McGrowder, Lowell L. Dilworth, Helen N. Asemota","doi":"10.1111/j.1755-9294.2011.01107.x","DOIUrl":"10.1111/j.1755-9294.2011.01107.x","url":null,"abstract":"<div>\u0000 \u0000 Background and aim: Studies have shown that polymethoxylated flavones have potent hypolipidemic properties. There are, however, gaps in the literature on their effects on organ structures. This study evaluated the histopathological effects of Ortanique peel polymethoxylated flavones extract (PMFort) on the organ structures of diet-induced hypercholesterolemic rats. Methods: Thirty Sprague–Dawley rats were fed normal rat chow as well as high cholesterol diets supplemented with PMFort and niacin, respectively. Light microscopic evaluation of the liver, spleen, kidney and bowel of hypercholesterolemic rats fed PMFort and niacin was performed to determine possible morphologic alterations, compared to untreated hypercholesterolemic rats. Results: Untreated hypercholesterolemic rats showed significantly altered intestinal morphology, with an increase in the number of absorptive cells and in villus length when compared to untreated normal rats. They also exhibited hepatic steatosis (100%) and inflammation (20%) and lymphoid hyperplasia of the spleen (33%). Hypercholesterolemic rats treated with 1.5% PMFort showed reductions in absorptive cell number, villus length and hepatic steatosis compared to the untreated hypercholesterolemic rats. They also showed significant reduction in splenic lymphoid hyperplasia. Conclusions: The current results would suggest that PMFort consumption has no toxic effects on the organs of hypercholesterolemic rats but may ameliorate hypercholesterolemia-associated alterations in organ structures.\u0000 </div>","PeriodicalId":92990,"journal":{"name":"Basic and applied pathology","volume":"4 3","pages":"71-77"},"PeriodicalIF":0.0,"publicationDate":"2011-07-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/j.1755-9294.2011.01107.x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"63684541","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 15

Mutations in isocitrate dehydrogenase isoforms 1 and 2 are rare events in primary central nervous system and non-central nervous system diffuse large B cell lymphoma 异柠檬酸脱氢酶亚型1和2的突变在原发性中枢神经系统和非中枢神经系统弥漫性大B细胞淋巴瘤中是罕见的

Basic and applied pathology Pub Date : 2011-06-14 DOI: 10.1111/j.1755-9294.2011.01105.x

Songmi Noh, Eun Ju Park, Junjeong Choi, Sarah Lee, Seon Jung Jang, Se Hoon Kim

{"title":"Mutations in isocitrate dehydrogenase isoforms 1 and 2 are rare events in primary central nervous system and non-central nervous system diffuse large B cell lymphoma","authors":"Songmi Noh, Eun Ju Park, Junjeong Choi, Sarah Lee, Seon Jung Jang, Se Hoon Kim","doi":"10.1111/j.1755-9294.2011.01105.x","DOIUrl":"10.1111/j.1755-9294.2011.01105.x","url":null,"abstract":"<div>\u0000 \u0000 Background and aim: Mutations in isocitrate dehydrogenase enzyme isoforms 1 (IDH1) and 2 (IDH2) have been identified in many cancers, including gliomas and acute myeloid leukemia. The aim of this study was to determine whether these genes are mutated in the primary central nervous system lymphoma (PCNSL). Methods: We analyzed IDH1 and IDH2 mutations in 20 PCNSL and 30 non-central nervous system (CNS) diffuse large B-cell lymphomas (DLBCLs) using routine formalin-fixed paraffin-embedded tissues and a polymerase chain reaction assay focusing on the known mutation hot spots, Arg 132 of IDH1 and Arg 172 of IDH2. Results: We did not detect mutations in IDH1 or IDH2 in PCNSL nor non-CNS DLBCL. Conclusions: Although IDH1 and IDH2 mutations are known to be important in gliomas and in some hematologic malignancies, they appear to be very rare events in PCNS and non-CNS DLBCLs. Further studies on other IDH mutations in larger, non-CNS DLBCL populations are needed.\u0000 </div>","PeriodicalId":92990,"journal":{"name":"Basic and applied pathology","volume":"4 2","pages":"58-62"},"PeriodicalIF":0.0,"publicationDate":"2011-06-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/j.1755-9294.2011.01105.x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"63684001","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Dissemination of knowledge on health: multiple resources and multiple steps 传播卫生知识:多种资源和多种步骤

Basic and applied pathology Pub Date : 2011-06-14 DOI: 10.1111/j.1755-9294.2011.01106.x

Jeong-Wook Seo MD, Fang An PhD, Young Mee Han MD, Dai Tao MD

引用次数: 1

Clinicopathologic significance of serum response factor expression in colorectal adenocarcinomas 结直肠腺癌血清反应因子表达的临床病理意义

Basic and applied pathology Pub Date : 2011-06-14 DOI: 10.1111/j.1755-9294.2011.01103.x

Se Min Jang, Young Jin Jun, Hulin Han, Kang Hong Lee, Ki-Seok Jang, Seung Sam Paik

{"title":"Clinicopathologic significance of serum response factor expression in colorectal adenocarcinomas","authors":"Se Min Jang, Young Jin Jun, Hulin Han, Kang Hong Lee, Ki-Seok Jang, Seung Sam Paik","doi":"10.1111/j.1755-9294.2011.01103.x","DOIUrl":"10.1111/j.1755-9294.2011.01103.x","url":null,"abstract":"<div>\u0000 \u0000 Background and aim: The purpose of this study was to investigate the role of serum response factor (SRF) expression and to evaluate its correlation with various clinicopathological parameters in colorectal adenocarcinomas. Methods: We used tissue microarrays consisting of 24 normal mucosa, 50 tubular adenomas, 496 adenocarcinomas, and 128 metastatic lesions. Results: The expression of SRF was rare in normal colonic mucosa with a mean expression score of 0.67 ± 0.17. Tubular adenomas had a mean expression score of 2.48 ± 0.31, adenocarcinomas 2.82 ± 0.13 and lymph node metastases 2.82 ± 0.36. Interestingly, SRF expression was high in distant metastases with a mean expression score of 4.83 ± 0.43. The mean SRF expression was increased significantly early in the normal-adenoma-carcinoma sequence and again in distant metastases. The positive SRF expression was strongly correlated with non-mucinous tumor type (P < 0.001). Moderately and poorly differentiated adenocarcinomas had higher mean expression scores than well-differentiated adenocarcinomas (2.88 ± 0.13 vs 1.33 ± 0.32) (P= 0.015). There were significant associations between SRF expression and expression of p53 (P= 0.034) and Ki-67 (P= 0.001). Conclusions: Our results suggest that SRF expression may be the early event of normal-adenoma-carcinoma sequence of colorectal cancer, especially in adenomatous change of colonic mucosa and may play an important role in distant metastasis of colorectal cancers.\u0000 </div>","PeriodicalId":92990,"journal":{"name":"Basic and applied pathology","volume":"4 2","pages":"46-52"},"PeriodicalIF":0.0,"publicationDate":"2011-06-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/j.1755-9294.2011.01103.x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"63683886","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Renal mucormycosis in immunocompetent patients: Report of three cases 免疫功能正常患者的肾毛霉病:附3例报告

Basic and applied pathology Pub Date : 2011-06-14 DOI: 10.1111/j.1755-9294.2011.01102.x

Ritu Verma, Mukul Vij, Vinita Agrawal, Manoj Jain

引用次数: 21

Pseudoxanthomatous salpingitis: Report of two cases with distinctive microscopical findings 假性黄瘤性输卵管炎:两例具有独特显微镜表现的报告

Basic and applied pathology Pub Date : 2011-06-14 DOI: 10.1111/j.1755-9294.2011.01104.x

Dimas Suárez-Vilela, Francisco Izquierdo, Jose Ramón Méndez, Juliana Escobar

{"title":"Pseudoxanthomatous salpingitis: Report of two cases with distinctive microscopical findings","authors":"Dimas Suárez-Vilela, Francisco Izquierdo, Jose Ramón Méndez, Juliana Escobar","doi":"10.1111/j.1755-9294.2011.01104.x","DOIUrl":"10.1111/j.1755-9294.2011.01104.x","url":null,"abstract":"<div>\u0000 \u0000 Background and aim: We describe two cases of pseudoxanthomatous salpingitis (PXS), an uncommon entity, often unknown for clinicians and pathologists. We review the literature on this topic and perform immunohistochemistry in order to study its pathogenesis. Methods: Both cases are studied by histochemical and immnunohistochemical methods with a wide panel of antibodies. Results: One case showed bilateral PXS, ovarian endometriosis and an adenomatoid tumor in the right tube. The second case showed a unilateral focus of tubal endometriosis. The first case displayed pigmented histiocytes, positive with periodic acid-Schiff (PAS), PAS with diastase digestion and focally with Fontana-Masson stains. Abundant hemosiderin granules were present in stromal subepithelial cells but no in histiocytes. In the second case Fontana-Masson and Ziehl-Neelsen methods stained diffusely the histiocytes. Perls stain was positive in some stromal cells and in numerous granular macrophages. The lymphocytic infiltrates presented mainly T cells with different proportions of CD4 and CD8 lymphocytes in each case. The tubal stroma was positive for CD10 in both cases. Conclusions: PXS is a rare condition usually associated with ovarian endometriosis. The physiopathologic way of pseudoxanthomatous histiocytes to accumulate inside the plicae of the tubes is not clear and can be diverse in different cases.\u0000 </div>","PeriodicalId":92990,"journal":{"name":"Basic and applied pathology","volume":"4 2","pages":"53-57"},"PeriodicalIF":0.0,"publicationDate":"2011-06-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/j.1755-9294.2011.01104.x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"63683980","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Wnt pathway is affected by endocrine therapy in breast carcinomas 乳腺癌内分泌治疗对Wnt通路的影响

Basic and applied pathology Pub Date : 2011-05-09 DOI: 10.1111/j.1755-9294.2011.01101.x

Yong Kyun Joo, Angela Flávia Logullo, André Mattar, Suely Nonogaki, Fernando Augusto Soares, Luíz Henrique Gebrim

{"title":"Wnt pathway is affected by endocrine therapy in breast carcinomas","authors":"Yong Kyun Joo, Angela Flávia Logullo, André Mattar, Suely Nonogaki, Fernando Augusto Soares, Luíz Henrique Gebrim","doi":"10.1111/j.1755-9294.2011.01101.x","DOIUrl":"10.1111/j.1755-9294.2011.01101.x","url":null,"abstract":"<div>\u0000 \u0000 Background and aim: Identifying potential predictive biomarkers of response to endocrine therapy would benefit most estrogen receptor-positive (ER+) breast cancer patients. Our aim was to compare the expression of the wingless type (Wnt) pathway-related proteins (adenomatous polyposis coli [APC], E-cadherin, beta-catenin, cyclin D1, and c-myc) in postmenopausal women with ER+ invasive ductal carcinomas (IDC), prior to and after tamoxifen (n= 18) or anastrozole (n= 15) treatment, in a double-blind, placebo-controlled (n= 25), prospective study for 26 days prior to surgery. Methods: Tissue microarray blocks were constructed from pre- and post-treatment biopsy samples. Nuclear immunostaining of c-myc, APC, estrogen and progesterone receptor levels were assessed using the Allred scoring system, and cytoplasmic immunostaining of cyclin D1, beta-catenin and E-cadherin was assessed with the Hercep-Test system. An anova statistical analysis estimated general equations and analysis of variance with a significance level of 0.05. Results: Tamoxifen increased c-myc (P= 0.0061) and APC (P= 0.0452) expression. Anastrozole did not significantly affect expression of any Wnt-related proteins. Conclusions: In post-menopausal ER+ IDC, tamoxifen for 26 days prior to IDC surgery influenced statistically the expression of important cell cycle regulators as APC and c-myc, whereas anastrozole therapy did not interfere with this pathway during the same period. These Wnt related proteins may contribute to selective estrogen receptor modulator resistance.\u0000 </div>","PeriodicalId":92990,"journal":{"name":"Basic and applied pathology","volume":"4 2","pages":"38-45"},"PeriodicalIF":0.0,"publicationDate":"2011-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/j.1755-9294.2011.01101.x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"63683976","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1