Insights of biomedical research最新文献

Endothelial Cell Activation Mediates Platelet Response to Pulsatile Shear Stress 内皮细胞活化介导血小板对脉动剪应力的反应

Insights of biomedical research Pub Date : 2022-01-17 DOI: 10.36959/584/461

Steadman Elisabeth, Bond Kyle, Laljee Sehar, F. Marina, R. David, Yin-Chu Wei

{"title":"Endothelial Cell Activation Mediates Platelet Response to Pulsatile Shear Stress","authors":"Steadman Elisabeth, Bond Kyle, Laljee Sehar, F. Marina, R. David, Yin-Chu Wei","doi":"10.36959/584/461","DOIUrl":"https://doi.org/10.36959/584/461","url":null,"abstract":"Thrombin plays critical roles in both hemostasis and thrombosis. Many studies were carried out to examine the effects of different thrombogenic surfaces on thrombin generation. In the present study, how activated endothelial cells affected platelet thrombin generation under dynamic shear stress was investigated. Human platelets were exposed to pulsatile shear stress (physiological, low or elevated) in a cone-and-plate shearing device for 30 minutes in the presence of quiescent or activated human coronary artery endothelial cells. Coagulation factors including Factor II, Factor Xa and calcium were immediately available. Thrombin generation rates were measured using a modified prothrombinase assay. Platelet expression of surface P- selectin, glycoprotein Ibα (GPIbα or CD42b), integrin αIIb (CD41a), and phosphatidylserine was measured using flow cytometry. Endothelial cell expression of intracellular adhesion molecule 1 (ICAM-1) and von Willebrand factor was measured using a solid-phase ELISA and fluorescence microscopy, respectively. Platelet adhesion to the endothelial cells was measured using fluorescence microscopy. The results indicated that the activation state of endothelial cells, but not the pulsatile shear stress, had a significant effect in thrombin generation rates. The activation state of endothelial cells also affected platelet surface GPIbα and P-selectin expression, which may have contributed to the overall change in thrombin generation rates.","PeriodicalId":92909,"journal":{"name":"Insights of biomedical research","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43084785","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Bilateral Subclavian CT Angiography via Lower Limb Contrast Injection 双侧锁骨下CT血管造影下肢注射造影剂

Insights of biomedical research Pub Date : 2022-01-01 DOI: 10.36959/584/464

S.M. Kim

引用次数: 0

Sensitizing Breast Cancer Cells Using Standard Microwave Devices and Temperature Monitoring With Non-Invasive Thermochemical Thermometers 使用标准微波装置和无创热化学温度计进行温度监测使乳腺癌细胞敏感

Insights of biomedical research Pub Date : 2022-01-01 DOI: 10.36959/584/463

L. Ounalli, M. Notter

引用次数: 0

Chemical and Physiological Change from Calcium Carbonate to Calcium Phosphate in Skeletal Structures 从碳酸钙到磷酸钙在骨骼结构中的化学生理变化

Insights of biomedical research Pub Date : 2021-01-23 DOI: 10.36959/584/460

H. W. Dee, Preskitt Caroline, Gresham-Fiegel Carolyn

{"title":"Chemical and Physiological Change from Calcium Carbonate to Calcium Phosphate in Skeletal Structures","authors":"H. W. Dee, Preskitt Caroline, Gresham-Fiegel Carolyn","doi":"10.36959/584/460","DOIUrl":"https://doi.org/10.36959/584/460","url":null,"abstract":"The substrates that comprise skeletal structures have evolved over more than 480 million years. We review chemical and physiological factors that may have driven the evolutionary process from an original calcium carbonate composition of skeletal structures to the calcium phosphate-based bone physiology that we observe in vertebrates today. A review of this topic is not without challenges, however. Many of these changes occurred nearly 480 million years ago, as evidenced by fossils from extinct creatures; these relics, and their correlations to extant organisms, are the only tools we have to develop insight into these changes [1]. In this paper, we discuss factors that may have contributed to the divergence of skeletal constituents to try to gain insights into this intriguing and notable physiological evolution. While the biology of these conditions is ultimately complex and no process can fully account for the millions of years of change, the goal is to propose a model and initiate a dialogue that could lead to, for example, targets for drug delivery, orthopedic treatment, prosthetics, bone pathologies, and other ideas for readers to build upon for future research and investigation. Locomotion and the storage and rapid retrieval of phosphorus, a critical mineral, provided a significant impetus for evolution. This review focuses on the chemical evolution toward CaPO4 from CaCO3 that addressed the need for 1) Storage of energy metabolites in the body and 2) Improved retrieval of these metabolites.","PeriodicalId":92909,"journal":{"name":"Insights of biomedical research","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44161891","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

TRANSCUP: A Scalable Workflow for Predicting Cancer of Unknown Primary Based on Next-Generation Transcriptome Profiling TRANSCUP:基于下一代转录组分析预测未知原发癌症的可扩展工作流程

Insights of biomedical research Pub Date : 2020-12-31 DOI: 10.36959/584/457

Li Peng

引用次数: 0

Prediction of Childhood Diarrhea in Bangladesh using Machine Learning Approach 用机器学习方法预测孟加拉国儿童腹泻

Insights of biomedical research Pub Date : 2020-12-31 DOI: 10.36959/584/456

Md. Maniruzzaman, Md. Shaykhul Islam, M. Abedin, M. Amanullah, Sadiq Hussain

{"title":"Prediction of Childhood Diarrhea in Bangladesh using Machine Learning Approach","authors":"Md. Maniruzzaman, Md. Shaykhul Islam, M. Abedin, M. Amanullah, Sadiq Hussain","doi":"10.36959/584/456","DOIUrl":"https://doi.org/10.36959/584/456","url":null,"abstract":"Diarrhea has remained a major health problem among under-five (U5) children that leads high level of morbidity and mortality. This study is to determine the socio-demographic risk factors of diarrhea as well as predict of diarrhea status using machine learning (ML) based approach among U5 children in Bangladesh. Bangladesh Demographic and Health Survey, 2014 dataset is used in this study. This dataset consisted of 7,538 respondents who had 371 (4.9%) child’s diarrhea. Logistic regression (LR) is used to determine the high-risk factors of diarrhea. Then four ML-based approach namely naïve Bayes (NB), linear discriminant analysis (LDA), quadratic discriminant analysis (QDA), and support vector machine (SVM) was applied to predict the child’s diarrhea status and accuracy, sensitivity, and specificity are used to evaluate the performance of these classifiers. Around 4.9% women reported that their children have experienced an episode of diarrhea in two weeks before the survey. LR model showed that the child’s age, region (Khulna and Rangpur), mothers who had completed secondary education, and respondents who were rich wealth index, significantly associated risk factors for diarrhea disease. Our findings indicate that SVM with radial basis kernel yielded 65.61% accuracy, 66.27% sensitivity, and 52.28% specificity which are comparatively better than others. The prevalence of diarrhea disease is more common among Bangladeshi children. Our study shows that SVM is capable of predicting child diarrhea status (generally highly imbalanced data). This study allows policy makers towards appropriate decisions to reduce childhood diarrhea in Bangladesh.","PeriodicalId":92909,"journal":{"name":"Insights of biomedical research","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45286121","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

An Approximate Overview of COVID-19 Treatment Approaches by Registered Physicians of Bangladesh 孟加拉国注册医生对COVID-19治疗方法的大致概述

Insights of biomedical research Pub Date : 2020-12-31 DOI: 10.36959/584/459

F. Rashid, M. Ashrafuzzaman, S. Ayesha, S. Islam, Niaz Mahmud, Md. Asadur Rahman

{"title":"An Approximate Overview of COVID-19 Treatment Approaches by Registered Physicians of Bangladesh","authors":"F. Rashid, M. Ashrafuzzaman, S. Ayesha, S. Islam, Niaz Mahmud, Md. Asadur Rahman","doi":"10.36959/584/459","DOIUrl":"https://doi.org/10.36959/584/459","url":null,"abstract":"Objectives: With the other countries around the world, Bangladesh is also fighting against the COVID-19 pandemic about which the current treatment and the medication procedure are not revealed worldwide. The aim of this study is to reveal the approximate treatment approaches served by the registered physicians of Bangladesh, especially for the last three months of coronavirus pandemic situation. Study design: The study design was set by generating questionnaires like multiple choice questions and dichotomous questions based on the current perspective of commonly used and different scholarly suggestions for treating corona patients. Methods: The working methodology was based on both qualitative research and descriptive research by evaluating the observed facts and explains the reasons behind them. Online Google questionnaire survey forms were used to collect data from the randomly picked fifty registered doctors (team leaders) of Bangladesh who are serving corona patients either in government or private health sectors during the last three months. The physicians selected have licenses from Bangladesh Medical and Dental Council (BMDC) and are authorized to prescribe medicines to COVID patients. Concerning our study, the physicians selected are specialized in medicines and mostly are Professors at different medical colleges or medical universities. Results: According to their clinical observations and practice experiences, this study found that Bangladeshi registered doctor’s treatment approaches for COVID-19 patients are scientifically co-related with the recent different researchbased work on treatment protocol to combat this coronavirus pandemic situation. Conclusions: Eventually our concluding remarks are regardless of various limitations in the health sectors of Bangladesh, the physicians have accurately followed the current research and WHO guidelines and are unconditionally serving a country of 165 million people for saving the nation from this deadly virus.","PeriodicalId":92909,"journal":{"name":"Insights of biomedical research","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47286458","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of Co-occurring Genomic Alterations on Survival in Patients with KRAS-Mutant Pancreatic Cancer 共同发生的基因组改变对kras突变胰腺癌患者生存的影响

Insights of biomedical research Pub Date : 2020-09-16 DOI: 10.36959/584/454

Moon Kangeun, Jae Kim Min, Chang Hyeonmin, D. Oh

引用次数: 0

An Overview of Sixteen Years Control Program against Rift Valley Fever in Saudi Arabia: A Review Study 沙特阿拉伯裂谷热16年防治计划综述：回顾性研究

Insights of biomedical research Pub Date : 2019-10-13 DOI: 10.36959/584/451

Alhaj Ms

引用次数: 1

Adrenal Lesion Core Biopsy: A New Approach and an Incidental Benefit 肾上腺病变核心活检:一种新方法和偶然的好处

Insights of biomedical research Pub Date : 2019-09-30 DOI: 10.36959/584/450

K. Smith

引用次数: 0