Biotechnology Research International最新文献_第8页

Optimization of the Nutritional Parameters for Enhanced Production of B. subtilis SPB1 Biosurfactant in Submerged Culture Using Response Surface Methodology. 响应面法优化枯草芽孢杆菌SPB1生物表面活性剂深层培养营养参数

Biotechnology Research International Pub Date : 2012-01-01 DOI: 10.1155/2012/795430

Ines Mnif, Semia Chaabouni-Ellouze, Dhouha Ghribi

{"title":"Optimization of the Nutritional Parameters for Enhanced Production of B. subtilis SPB1 Biosurfactant in Submerged Culture Using Response Surface Methodology.","authors":"Ines Mnif, Semia Chaabouni-Ellouze, Dhouha Ghribi","doi":"10.1155/2012/795430","DOIUrl":"https://doi.org/10.1155/2012/795430","url":null,"abstract":"Nutritional requirements can contribute considerably to the production cost and the bioprocess economics. Media optimisation using response surface methodology is one of the used methods to ameliorate the bioprocess economics. In the present study, biosurfactant production by Bacillus subtilis SPB1 was effectively enhanced by response surface methodology. A Plackett-Burman-based statistical screening procedure was adopted to determine the most important factor affecting lipopeptide production. Eleven variables are screened and results show that glucose, K(2)HPO(4), and urea concentrations influence the most biosurfactant production. A Central Composite Design was conducted to optimize the three selected factors. Statistical analyses of the data of model fitting were done by using NemrodW. Results show a maximum predicted biosurfactant concentration of 2.93 (±0.32) g/L when using 15 g/L glucose, 6 g/L urea, and 1 g/L K(2)HPO(4). The predicted value is approximately 1.65 much higher than the original production determined by the conventional one-factor-at-a-time optimization method.","PeriodicalId":9268,"journal":{"name":"Biotechnology Research International","volume":"2012 ","pages":"795430"},"PeriodicalIF":0.0,"publicationDate":"2012-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2012/795430","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9376240","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 45

Bioprocessing of "Hair Waste" by Paecilomyces lilacinus as a Source of a Bleach-Stable, Alkaline, and Thermostable Keratinase with Potential Application as a Laundry Detergent Additive: Characterization and Wash Performance Analysis. 淡紫色拟青霉对“头发废物”进行生物处理，作为一种具有漂白、碱性和热稳定性的角蛋白酶，并有可能应用于洗衣添加剂:表征和洗涤性能分析。

Biotechnology Research International Pub Date : 2012-01-01 DOI: 10.1155/2012/369308

Ivana A Cavello, Roque A Hours, Sebastián F Cavalitto

{"title":"Bioprocessing of \"Hair Waste\" by Paecilomyces lilacinus as a Source of a Bleach-Stable, Alkaline, and Thermostable Keratinase with Potential Application as a Laundry Detergent Additive: Characterization and Wash Performance Analysis.","authors":"Ivana A Cavello, Roque A Hours, Sebastián F Cavalitto","doi":"10.1155/2012/369308","DOIUrl":"https://doi.org/10.1155/2012/369308","url":null,"abstract":"Paecilomyces lilacinus (Thom) Samson LPS 876, a locally isolated fungal strain, was grown on minimal mineral medium containing \"hair waste,\" a residue from the hair-saving unhairing process, and produced a protease with keratinolytic activity. This enzyme was biochemically characterized. The optimum reaction conditions, determined with a response surface methodology, were 60°C and pH 6.0. It was remarkably stable in a wide range of pHs and temperatures. Addition of Ca(2+), Mg(2+), or sorbitol was found to be effective in increasing thermal stability of the protease. PMSF and Hg(2+) inhibited the proteolytic activity indicating the presence of a thiol-dependent serine protease. It showed high stability toward surfactants, bleaching agents, and solvents. It was also compatible with commercial detergents (7 mg/mL) such as Ariel, Skip, Drive, and Ace, retaining more than 70% of its proteolytic activity in all detergents after 1 h of incubation at 40°C. Wash performance analysis revealed that this protease could effectively remove blood stains. From these properties, this enzyme may be considered as a potential candidate for future use in biotechnological processes, as well as in the formulation of laundry detergents.","PeriodicalId":9268,"journal":{"name":"Biotechnology Research International","volume":"2012 ","pages":"369308"},"PeriodicalIF":0.0,"publicationDate":"2012-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2012/369308","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9376773","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 25

Experimental Investigations on the Effects of Carbon and Nitrogen Sources on Concomitant Amylase and Polygalacturonase Production by Trichoderma viride BITRS-1001 in Submerged Fermentation. 碳氮源对绿木霉BITRS-1001深层发酵产伴随淀粉酶和聚半乳糖醛酸酶影响的实验研究

Biotechnology Research International Pub Date : 2012-01-01 DOI: 10.1155/2012/904763

Arotupin Daniel Juwon, Ogunmolu Funso Emmanuel

引用次数: 32

High-Frequency Regeneration of the Drought-Tolerant Tree Melia volkensii Gurke Using Low-Cost Agrochemical Thidiazuron. 低成本农化剂噻唑脲对抗旱树种白杨高频再生的影响。

Biotechnology Research International Pub Date : 2012-01-01 DOI: 10.1155/2012/818472

Eliud Sagwa Mulanda, Mark Ochieng Adero, Nelson Onzere Amugune, Elijah Akunda, Jenesio I Kinyamario

{"title":"High-Frequency Regeneration of the Drought-Tolerant Tree Melia volkensii Gurke Using Low-Cost Agrochemical Thidiazuron.","authors":"Eliud Sagwa Mulanda, Mark Ochieng Adero, Nelson Onzere Amugune, Elijah Akunda, Jenesio I Kinyamario","doi":"10.1155/2012/818472","DOIUrl":"https://doi.org/10.1155/2012/818472","url":null,"abstract":"Melia volkensii Gurke is a drought-tolerant tree native to East Africa's arid and semiarid lands (ASALs), with vast but underutilized potential for agroforestry and sustainable livelihoods in the ASALs. Its cultivation is limited by difficulties in propagation via conventional means. Full exploitation of the ability of thidiazuron (TDZ) to elicit regeneration in plant tissue cultures, as sole plant growth regulator (PGR), is hampered by high costs. This study tested the effectiveness of a low-cost agrochemical TDZ for in vitro propagation of M. volkensii. Zygotic embryos from mature seeds were cultured on Gamborg's B5 medium containing 0 to 4 mg/L of agrochemical TDZ from Kingtai Chemicals Co.,Ltd., China. Callus induction frequency was 96.67 to 100%. Significantly large callus fresh mass was produced at 0.05 mg/L TDZ concentration (ANOVA, P < 0.001). The effect of TDZ on embryogenicity was significant over certain ranges of concentrations (Anova, P < 0.001). Multiple somatic embryos developed within 14 days of subculture to hormone-free B5 medium. Somatic embryos developed into microshoots which elongated when transferred to 1/2 MS medium supplemented with 0.1 mg/L 6-benzylaminopurine plus 10% coconut water. The Kingtai-TDZ showed a high potency and suitability for use in M. volkensii tissue culture.","PeriodicalId":9268,"journal":{"name":"Biotechnology Research International","volume":"2012 ","pages":"818472"},"PeriodicalIF":0.0,"publicationDate":"2012-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2012/818472","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9744712","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 12

Biotechnological tools for environmental sustainability: prospects and challenges for environments in Nigeria-a standard review. 促进环境可持续性的生物技术工具:尼日利亚环境的前景和挑战-标准审查。

Biotechnology Research International Pub Date : 2012-01-01 DOI: 10.1155/2012/450802

Chukwuma S Ezeonu, Richard Tagbo, Ephraim N Anike, Obinna A Oje, Ikechukwu N E Onwurah

引用次数: 60

Cellulosic Ethanol Production by Recombinant Cellulolytic Bacteria Harbouring pdc and adh II Genes of Zymomonas mobilis. 含有活动单胞菌pdc和adh II基因的重组纤维素分解菌生产纤维素乙醇。

Biotechnology Research International Pub Date : 2012-01-01 DOI: 10.1155/2012/817549

P Sobana Piriya, P Thirumalai Vasan, V S Padma, U Vidhyadevi, K Archana, S John Vennison

{"title":"Cellulosic Ethanol Production by Recombinant Cellulolytic Bacteria Harbouring pdc and adh II Genes of Zymomonas mobilis.","authors":"P Sobana Piriya, P Thirumalai Vasan, V S Padma, U Vidhyadevi, K Archana, S John Vennison","doi":"10.1155/2012/817549","DOIUrl":"https://doi.org/10.1155/2012/817549","url":null,"abstract":"The ethanol fermenting genes such as pyruvate decarboxylase (pdc) and alcohol dehydrogenase II (adh II) were cloned from Zymomonas mobilis and transformed into three different cellulolytic bacteria, namely Enterobacter cloacae JV, Proteus mirabilis JV and Erwinia chrysanthemi and their cellulosic ethanol production capability was studied. Recombinant E. cloacae JV was found to produce 4.5% and 3.5% (v/v) ethanol, respectively, when CMC and 4% NaOH pretreated bagasse were used as substrates, whereas recombinant P. mirabilis and E. chrysanthemi with the same substrates could only produce 4%, 3.5%, 1%, and 1.5 % of ethanol, respectively. The recombinant E. cloacae strain produced twofold higher percentage of ethanol than the wild type. The recombinant E. cloacae strain could be improved further by increasing its ethanol tolerance capability through media optimization and also by combining multigene cellulase expression for enhancing ethanol production from various types of lignocellulosic biomass so that it can be used for industrial level ethanol production.","PeriodicalId":9268,"journal":{"name":"Biotechnology Research International","volume":"2012 ","pages":"817549"},"PeriodicalIF":0.0,"publicationDate":"2012-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2012/817549","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9432595","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 17

The diversity of endophytic methylotrophic bacteria in an oil-contaminated and an oil-free mangrove ecosystem and their tolerance to heavy metals. 含油和无油红树林生态系统内生甲基营养细菌的多样性及其对重金属的耐受性。

Biotechnology Research International Pub Date : 2012-01-01 DOI: 10.1155/2012/759865

Manuella Nobrega Dourado, Anderson Ferreira, Welington Luiz Araújo, João Lúcio Azevedo, Paulo Teixeira Lacava

{"title":"The diversity of endophytic methylotrophic bacteria in an oil-contaminated and an oil-free mangrove ecosystem and their tolerance to heavy metals.","authors":"Manuella Nobrega Dourado, Anderson Ferreira, Welington Luiz Araújo, João Lúcio Azevedo, Paulo Teixeira Lacava","doi":"10.1155/2012/759865","DOIUrl":"https://doi.org/10.1155/2012/759865","url":null,"abstract":"Methylobacterium strains were isolated from mangrove samples collected in Bertioga, SP, Brazil, from locations either contaminated or uncontaminated by oil spills. The tolerances of the strains to different heavy metals were assessed by exposing them to different concentrations of cadmium, lead, and arsenic (0.1 mM, 0.5 mM, 1 mM, 2 mM, 4 mM, and 8 mM). Additionally, the genetic diversity of Methylobacterium spp. was determined by sequence analysis of the 16S rRNA genes. The isolates from the contaminated locations were grouped, suggesting that oil can select for microorganisms that tolerate oil components and can change the methylotrophic bacterial community. Cadmium is the most toxic heavy metal assessed in this work, followed by arsenic and lead, and two isolates of Methylobacterium were found to be tolerant to all three metals. These isolates have the potential to bioremediate mangrove environments contaminated by oil spills by immobilizing the heavy metals present in the oil.","PeriodicalId":9268,"journal":{"name":"Biotechnology Research International","volume":"2012 ","pages":"759865"},"PeriodicalIF":0.0,"publicationDate":"2012-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2012/759865","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9374551","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 47

Properties and therapeutic application of bromelain: a review. 菠萝蛋白酶的特性和治疗应用：综述。

Biotechnology Research International Pub Date : 2012-01-01 Epub Date: 2012-12-10 DOI: 10.1155/2012/976203

Rajendra Pavan, Sapna Jain, Shraddha, Ajay Kumar

{"title":"Properties and therapeutic application of bromelain: a review.","authors":"Rajendra Pavan, Sapna Jain, Shraddha, Ajay Kumar","doi":"10.1155/2012/976203","DOIUrl":"10.1155/2012/976203","url":null,"abstract":"Bromelain belongs to a group of protein digesting enzymes obtained commercially from the fruit or stem of pineapple. Fruit bromelain and stem bromelainare prepared differently and they contain different enzymatic composition. \"Bromelain\" refers usually to the \"stem bromelain.\" Bromelain is a mixture of different thiol endopeptidases and other components like phosphatase, glucosidase, peroxidase, cellulase, escharase, and several protease inhibitors. In vitro and in vivo studies demonstrate that bromelain exhibits various fibrinolytic, antiedematous, antithrombotic, and anti-inflammatory activities. Bromelain is considerably absorbable in the body without losing its proteolytic activity and without producing any major side effects. Bromelain accounts for many therapeutic benefits like the treatment of angina pectoris, bronchitis, sinusitis, surgical trauma, and thrombophlebitis, debridement of wounds, and enhanced absorption of drugs, particularly antibiotics. It also relieves osteoarthritis, diarrhea, and various cardiovascular disorders. Bromelain also possesses some anticancerous activities and promotes apoptotic cell death. This paper reviews the important properties and therapeutic applications of bromelain, along with the possible mode of action.","PeriodicalId":9268,"journal":{"name":"Biotechnology Research International","volume":"2012 ","pages":"976203"},"PeriodicalIF":0.0,"publicationDate":"2012-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3529416/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9375629","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Isolation and characterization of some phytochemicals from Indian traditional plants. 印度传统植物中某些植物化学物质的分离与鉴定。

Biotechnology Research International Pub Date : 2012-01-01 DOI: 10.1155/2012/549850

Neeharika Srivastava, Aishwarya Singh Chauhan, Bechan Sharma

{"title":"Isolation and characterization of some phytochemicals from Indian traditional plants.","authors":"Neeharika Srivastava, Aishwarya Singh Chauhan, Bechan Sharma","doi":"10.1155/2012/549850","DOIUrl":"https://doi.org/10.1155/2012/549850","url":null,"abstract":"The present study was designed to evaluate relative contribution of different polyphenols (total phenolics, flavonoids, flavonols) and their antioxidants activities in aqueous extracts of different parts of some plants; Argemone mexicana, Datura metel, Calotropis procera, Thevetia peruviana, and Cannabis sativa. The antioxidants (total phenolics, flavonoids, flavones) were determined by chemical methods. The antioxidant capacities of these extracts were evaluated by FRAP assay. The results demonstrated that phenolic content was maximally present in leaves of T. peruviana. This plant exhibited minimum phenolic content in its flower as compared to other plants. The flower of D. metel contained maximum phenolic content. The flavonoids were present in highest quantity in leaves of C. procera while T. peruviana flowers showed maximum flavonoid content. The fruits of C. sativa contained maximum quantity of flavonoid as compared to other plants tested. The flower extract of C. sativa possessed highest FRAP value followed by A. mexicana and fruit of C. procera. The values of ratios of different polyphenolic compounds present in plant extracts indicated that flower of D. metel contained maximum total flavonoids and minimum phenolics. These results suggested that levels of total phenolics, flavonoids and their FRAP indices exhibited specificity to different plants and their parts.","PeriodicalId":9268,"journal":{"name":"Biotechnology Research International","volume":"2012 ","pages":"549850"},"PeriodicalIF":0.0,"publicationDate":"2012-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2012/549850","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9368604","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 50

Recent advances in the genetic transformation of coffee. 咖啡基因转化的最新进展。

Biotechnology Research International Pub Date : 2012-01-01 DOI: 10.1155/2012/580857

M K Mishra, A Slater

引用次数: 105