Biotechnology Research International最新文献_第7页

Antioxidant and Hepatoprotective Properties of Tofu (Curdle Soymilk) against Acetaminophen-Induced Liver Damage in Rats. 豆腐（凝固豆浆）对对乙酰氨基酚引起的大鼠肝损伤的抗氧化和保肝作用

Biotechnology Research International Pub Date : 2013-01-01 Epub Date: 2013-02-28 DOI: 10.1155/2013/230142

Ndatsu Yakubu, Ganiyu Oboh, Amuzat Aliyu Olalekan

{"title":"Antioxidant and Hepatoprotective Properties of Tofu (Curdle Soymilk) against Acetaminophen-Induced Liver Damage in Rats.","authors":"Ndatsu Yakubu, Ganiyu Oboh, Amuzat Aliyu Olalekan","doi":"10.1155/2013/230142","DOIUrl":"10.1155/2013/230142","url":null,"abstract":"The antioxidant and hepatoprotective properties of tofu using acetaminophen to induce liver damage in albino rats were evaluated. Tofus were prepared using calcium chloride, alum, and steep water as coagulants. The polyphenols of tofu were extracted and their antioxidant properties were determined. The weight gain and feed intake of the rats were measured. The analysis of serum alanine aminotransferase (ALT), alkaline phosphatase (ALP), aspartate aminotransferase (AST), and lactate dehydrogenase (LDH) activities and the concentrations of albumin, total protein, cholesterol, and bilirubin were analyzed. The result reveals that the antioxidant property of both soluble and bound polyphenolic extracts was significantly higher in all tofus, but the steep water coagulated tofu was recorded higher. Rats fed with various tofus and acetaminophen had their serum ALP, ALT, AST, and LDH activities; total cholesterol; and bilirubin levels significantly (P < 0.05) reduced, and total protein and albumin concentrations increased when compared with basal diet and acetaminophen administered group. Therefore, all tofus curdled with various coagulants could be used to prevent liver damage caused by oxidative stress.","PeriodicalId":9268,"journal":{"name":"Biotechnology Research International","volume":" ","pages":"230142"},"PeriodicalIF":0.0,"publicationDate":"2013-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3600259/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40228636","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Structural Variations of Human Glucokinase Glu256Lys in MODY2 Condition Using Molecular Dynamics Study. MODY2条件下人葡萄糖激酶Glu256Lys结构变化的分子动力学研究

Biotechnology Research International Pub Date : 2013-01-01 Epub Date: 2013-02-13 DOI: 10.1155/2013/264793

Nanda Kumar Yellapu, Kalpana Kandlapalli, Koteswara Rao Valasani, P V G K Sarma, Bhaskar Matcha

{"title":"Structural Variations of Human Glucokinase Glu256Lys in MODY2 Condition Using Molecular Dynamics Study.","authors":"Nanda Kumar Yellapu, Kalpana Kandlapalli, Koteswara Rao Valasani, P V G K Sarma, Bhaskar Matcha","doi":"10.1155/2013/264793","DOIUrl":"https://doi.org/10.1155/2013/264793","url":null,"abstract":"Glucokinase (GK) is the predominant hexokinase that acts as glucose sensor and catalyses the formation of Glucose-6-phosphate. The mutations in GK gene influence the affinity for glucose and lead to altered glucose levels in blood causing maturity onset diabetes of the young type 2 (MODY2) condition, which is one of the prominent reasons of type 2 diabetic condition. In view of the importance of mutated GK resulting in hyperglycemic condition, in the present study, molecular dynamics simulations were carried out in intact and 256 E-K mutated GK structures and their energy values and conformational variations were correlated. Energy variations were observed in mutated GK (3500 Kcal/mol) structure with respect to intact GK (5000 Kcal/mol), and it showed increased γ -turns, decreased β -turns, and more helix-helix interactions that affected substrate binding region where its volume increased from 1089.152 Å(2) to 1246.353 Å(2). Molecular docking study revealed variation in docking scores (intact = -12.199 and mutated = -8.383) and binding mode of glucose in the active site of mutated GK where the involvement of A53, S54, K56, K256, D262 and Q286 has resulted in poor glucose binding which probably explains the loss of catalytic activity and the consequent prevailing of high glucose levels in MODY2 condition.","PeriodicalId":9268,"journal":{"name":"Biotechnology Research International","volume":"2013 ","pages":"264793"},"PeriodicalIF":0.0,"publicationDate":"2013-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2013/264793","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"31295119","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Display of the Viral Epitopes on Lactococcus lactis: A Model for Food Grade Vaccine against EV71. 显示病毒抗原表位的Lactococcus lactis:食品级EV71疫苗的典范。

Biotechnology Research International Pub Date : 2013-01-01 Epub Date: 2013-02-13 DOI: 10.1155/2013/431315

Nadimpalli Ravi S Varma, Haryanti Toosa, Hooi Ling Foo, Noorjahan Banu Mohamed Alitheen, Mariana Nor Shamsudin, Ali S Arbab, Khatijah Yusoff, Raha Abdul Rahim

{"title":"Display of the Viral Epitopes on Lactococcus lactis: A Model for Food Grade Vaccine against EV71.","authors":"Nadimpalli Ravi S Varma, Haryanti Toosa, Hooi Ling Foo, Noorjahan Banu Mohamed Alitheen, Mariana Nor Shamsudin, Ali S Arbab, Khatijah Yusoff, Raha Abdul Rahim","doi":"10.1155/2013/431315","DOIUrl":"https://doi.org/10.1155/2013/431315","url":null,"abstract":"In this study, we have developed a system for display of antigens of Enterovirus type 71 (EV71) on the cell surface of L. lactis. The viral capsid protein (VP1) gene from a local viral isolate was utilized as the candidate vaccine for the development of oral live vaccines against EV71 using L. lactis as a carrier. We expressed fusion proteins in E. coli and purified fusion proteins were incubated with L. lactis. We confirmed that mice orally fed with L. lactis displaying these fusion proteins on its surface were able to mount an immune response against the epitopes of EV71. This is the first example of an EV71 antigen displayed on the surface of a food grade organism and opens a new perspective for alternative vaccine strategies against the EV71. We believe that the method of protein docking utilized in this study will allow for more flexible presentations of short peptides and proteins on the surface of L. lactis to be useful as a delivery vehicle.","PeriodicalId":9268,"journal":{"name":"Biotechnology Research International","volume":"2013 ","pages":"431315"},"PeriodicalIF":0.0,"publicationDate":"2013-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2013/431315","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"31295121","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 11

Production of alkaline cellulase by fungi isolated from an undisturbed rain forest of peru. 从秘鲁原始雨林中分离的真菌生产碱性纤维素酶。

Biotechnology Research International Pub Date : 2012-01-01 DOI: 10.1155/2012/934325

Karin Vega, Gretty K Villena, Victor H Sarmiento, Yvette Ludeña, Nadia Vera, Marcel Gutiérrez-Correa

{"title":"Production of alkaline cellulase by fungi isolated from an undisturbed rain forest of peru.","authors":"Karin Vega, Gretty K Villena, Victor H Sarmiento, Yvette Ludeña, Nadia Vera, Marcel Gutiérrez-Correa","doi":"10.1155/2012/934325","DOIUrl":"https://doi.org/10.1155/2012/934325","url":null,"abstract":"Alkaline cellulase producing fungi were isolated from soils of an undisturbed rain forest of Peru. The soil dilution plate method was used for the enumeration and isolation of fast growing cellulolytic fungi on an enriched selective medium. Eleven out of 50 different morphological colonies were finally selected by using the plate clearing assay with CMC as substrate at different pH values. All 11 strains produced cellulases in liquid culture with activities at alkaline pH values without an apparent decrease of them indicating that they are true alkaline cellulase producers. Aspergillus sp. LM-HP32, Penicillium sp. LM-HP33, and Penicillium sp. LM-HP37 were the best producers of FP cellulase (>3 U mL(-1)) with higher specific productivities (>30 U g(-1) h(-1)). Three strains have been found suitable for developing processes for alkaline cellulase production. Soils from Amazonian rain forests are good sources of industrial fungi with particular characteristics. The results of the present study are of commercial and biological interest. Alkaline cellulases may be used in the polishing and washing of denim processing of the textile industry.","PeriodicalId":9268,"journal":{"name":"Biotechnology Research International","volume":"2012 ","pages":"934325"},"PeriodicalIF":0.0,"publicationDate":"2012-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2012/934325","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9368590","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 38

Production of a Thermostable and Alkaline Chitinase by Bacillus thuringiensis subsp. kurstaki Strain HBK-51. 苏云金芽孢杆菌产耐热碱性几丁质酶的研究。kurstaki菌株HBK-51。

Biotechnology Research International Pub Date : 2012-01-01 DOI: 10.1155/2012/135498

Secil Berna Kuzu, Hatice Korkmaz Güvenmez, Aziz Akin Denizci

{"title":"Production of a Thermostable and Alkaline Chitinase by Bacillus thuringiensis subsp. kurstaki Strain HBK-51.","authors":"Secil Berna Kuzu, Hatice Korkmaz Güvenmez, Aziz Akin Denizci","doi":"10.1155/2012/135498","DOIUrl":"https://doi.org/10.1155/2012/135498","url":null,"abstract":"This paper reports the isolation and identification of chitinase-producing Bacillus from chitin-containing wastes, production of a thermostable and alkaline chitinasese, and enzyme characterization. Bacillus thuringiensis subsp. kurstaki HBK-51 was isolated from soil and was identified. Chitinase was obtained from supernatant of B. thuringiensis HBK-51 strain and showed its optimum activity at 110°C and at pH 9.0. Following 3 hours of incubation period, the enzyme showed a high level of activity at 110°C (96% remaining activity) and between pH 9.0 and 12.0 (98% remaining activity). Considering these characteristics, the enzyme was described as hyperthermophile-thermostable and highly alkaline. Two bands of the enzyme weighing 50 and 125 kDa were obtained following 12% SDS-PAGE analyses. Among the metal ions and chemicals used, Ni(2+) (32%), K(+) (44%), and Cu(2+) (56%) increased the enzyme activity while EDTA (7%), SDS (7%), Hg(2+) (11%), and ethyl-acetimidate (20%) decreased the activity of the enzyme. Bacillus thuringiensis subsp. kurstaki HBK-51 is an important strain which can be used in several biotechnological applications as a chitinase producer.","PeriodicalId":9268,"journal":{"name":"Biotechnology Research International","volume":"2012 ","pages":"135498"},"PeriodicalIF":0.0,"publicationDate":"2012-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2012/135498","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9368606","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 45

Functional Cloning and Expression of the Schizophyllum commune Glucuronoyl Esterase Gene and Characterization of the Recombinant Enzyme. 裂叶菌葡糖醛酸酯酶基因的功能克隆、表达及重组酶的鉴定。

Biotechnology Research International Pub Date : 2012-01-01 DOI: 10.1155/2012/951267

Dominic W S Wong, Victor J Chan, Amanda A McCormack, Ján Hirsch, Peter Biely

引用次数: 20

Optimization of Fermentation Medium for the Production of Glucose Isomerase Using Streptomyces sp. SB-P1. 链霉菌SB-P1产葡萄糖异构酶发酵培养基的优化

Biotechnology Research International Pub Date : 2012-01-01 DOI: 10.1155/2012/874152

Sheetal Bhasin, H A Modi

{"title":"Optimization of Fermentation Medium for the Production of Glucose Isomerase Using Streptomyces sp. SB-P1.","authors":"Sheetal Bhasin, H A Modi","doi":"10.1155/2012/874152","DOIUrl":"https://doi.org/10.1155/2012/874152","url":null,"abstract":"The combination of medium ingredients has a profound influence on the metabolic pathways running in the microorganism which regulates the production of numerous metabolites. Glucose isomerase (GI), an enzyme with huge potential in the market, can isomerise glucose into fructose. GI is used widely for the production of High-Fructose Corn Syrup (HFCS). HFCS is used as a sweetener in food and pharmaceutical industries. Streptomyces are well-known producers of numerous enzymes including glucose isomerase. An array of 75 isolates was screened for the production of glucose isomerase. The isolate Streptomyces sp. SB-P1 was found to produce maximum amount of extracellular GI. Sucrose and raffinose among pure carbon sources and corn cob and wheat husk among crude agro residues were found to yield high enzyme titers. Potassium nitrate among pure nitrogen sources and soy residues among crude sources gave maximum production. Quantitative effect of carbon, nitrogen, and inducer on GI was also determined. Plackett-Burman design was used to study the effect of different medium ingredients. Sucrose and xylose as carbon sources and peptone and soy residues as nitrogen sources proved to be beneficial for GI production.","PeriodicalId":9268,"journal":{"name":"Biotechnology Research International","volume":"2012 ","pages":"874152"},"PeriodicalIF":0.0,"publicationDate":"2012-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2012/874152","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9368102","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 27

DNA Damage Protecting Activity and Free Radical Scavenging Activity of Anthocyanins from Red Sorghum (Sorghum bicolor) Bran. 红高粱(高粱双色)麸皮花青素的DNA损伤保护活性和自由基清除活性。

Biotechnology Research International Pub Date : 2012-01-01 DOI: 10.1155/2012/258787

P Suganya Devi, M Saravana Kumar, S Mohan Das

引用次数: 40

Biodegradation of used motor oil in soil using organic waste amendments. 利用有机废物改良剂在土壤中降解废旧机油。

Biotechnology Research International Pub Date : 2012-01-01 Epub Date: 2012-06-20 DOI: 10.1155/2012/587041

O P Abioye, P Agamuthu, A R Abdul Aziz

{"title":"Biodegradation of used motor oil in soil using organic waste amendments.","authors":"O P Abioye, P Agamuthu, A R Abdul Aziz","doi":"10.1155/2012/587041","DOIUrl":"https://doi.org/10.1155/2012/587041","url":null,"abstract":"Soil and surface water contamination by used lubricating oil is a common occurrence in most developing countries. This has been shown to have harmful effects on the environment and human beings at large. Bioremediation can be an alternative green technology for remediation of such hydrocarbon-contaminated soil. Bioremediation of soil contaminated with 5% and 15% (w/w) used lubricating oil and amended with 10% brewery spent grain (BSG), banana skin (BS), and spent mushroom compost (SMC) was studied for a period of 84 days, under laboratory condition. At the end of 84 days, the highest percentage of oil biodegradation (92%) was recorded in soil contaminated with 5% used lubricating oil and amended with BSG, while only 55% of oil biodegradation was recorded in soil contaminated with 15% used lubricating oil and amended with BSG. Results of first-order kinetic model to determine the rate of biodegradation of used lubricating oil revealed that soil amended with BSG recorded the highest rate of oil biodegradation (0.4361 day(-1)) in 5% oil pollution, while BS amended soil recorded the highest rate of oil biodegradation (0.0556 day(-1)) in 15% oil pollution. The results of this study demonstrated the potential of BSG as a good substrate for enhanced remediation of hydrocarbon contaminated soil at low pollution concentration.","PeriodicalId":9268,"journal":{"name":"Biotechnology Research International","volume":" ","pages":"587041"},"PeriodicalIF":0.0,"publicationDate":"2012-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2012/587041","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"30856175","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 143

Involvement of the ligninolytic system of white-rot and litter-decomposing fungi in the degradation of polycyclic aromatic hydrocarbons. 白腐和凋落物分解真菌木质素分解系统在多环芳烃降解中的作用。

Biotechnology Research International Pub Date : 2012-01-01 DOI: 10.1155/2012/243217

Natalia N Pozdnyakova

引用次数: 92