Electrospinning最新文献

筛选

英文中文

Modeling early stage bone regeneration with biomimetic electrospun fibrinogen nanofibers and adipose-derived mesenchymal stem cells 用仿生电纺丝纤维蛋白原纳米纤维和脂肪来源的间充质干细胞模拟早期骨再生

Electrospinning Pub Date : 2016-01-04 DOI: 10.1515/esp-2016-0002

Michael P. Francis, Yas M. Moghaddam-White, Patrick C. Sachs, M. Beckman, Stephen M. Chen, G. Bowlin, L. Elmore, S. Holt

{"title":"Modeling early stage bone regeneration with biomimetic electrospun fibrinogen nanofibers and adipose-derived mesenchymal stem cells","authors":"Michael P. Francis, Yas M. Moghaddam-White, Patrick C. Sachs, M. Beckman, Stephen M. Chen, G. Bowlin, L. Elmore, S. Holt","doi":"10.1515/esp-2016-0002","DOIUrl":"https://doi.org/10.1515/esp-2016-0002","url":null,"abstract":"Abstract The key events of the earliest stages of bone regeneration have been described in vivo although not yet modeled in an in vitro environment, where mechanistic cell-matrix-growth factor interactions can be more effectively studied. Here, we explore an early-stage bone regeneration model where the ability of electrospun fibrinogen (Fg) nanofibers to regulate osteoblastogenesis between distinct mesenchymal stem cells populations is assessed. Electrospun scaffolds of Fg, polydioxanone (PDO), and a Fg:PDO blend were seeded with adipose-derived mesenchymal stem cells (ASCs) and grown for 7-21 days in osteogenic differentiation media or control growth media. Scaffolds were analyzed weekly for histologic and molecular evidence of osteoblastogenesis. In response to osteogenic differentiation media, ASCs seeded on the Fg scaffolds exhibit elevated expression of multiple genes associated with osteoblastogenesis. Histologic stains and scanning electron microscopy demonstrate widespread mineralization within the scaffolds, as well as de novo type I collagen synthesis. Our data demonstrates that electrospun Fg nanofibers support ASC osteogenic differentiation, yet the scaffold itself does not appear to be osteoinductive. Together, ASCs and Fg recapitulate early stages of bone regeneration ex vivo and presents a prospective autologous therapeutic approach for bone repair.","PeriodicalId":92629,"journal":{"name":"Electrospinning","volume":"53 1","pages":"10 - 19"},"PeriodicalIF":0.0,"publicationDate":"2016-01-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1515/esp-2016-0002","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67352069","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

Sub-micron sized saccharide fibres via electrospinning 通过静电纺丝制备亚微米大小的糖纤维

Electrospinning Pub Date : 2015-12-01 DOI: 10.1515/esp-2016-0001

Pablo G. T. Lepe, N. Tucker, Lyall D. Simmons, A. Watson, A. Fairbanks, M. Staiger

{"title":"Sub-micron sized saccharide fibres via electrospinning","authors":"Pablo G. T. Lepe, N. Tucker, Lyall D. Simmons, A. Watson, A. Fairbanks, M. Staiger","doi":"10.1515/esp-2016-0001","DOIUrl":"https://doi.org/10.1515/esp-2016-0001","url":null,"abstract":"Abstract In this work, the production of continuous submicron diameter saccharide fibres is shown to be possible using the electrospinning process. The mechanism for the formation of electrospun polymer fibres is usually attributed to the physical entanglement of long molecular chains. The ability to electrospin continuous fibre from a low molecular weight saccharides was an unexpected phenomenon. The formation of sub-micron diameter “sugar syrup” fibres was observed in situ using highspeed video. The trajectory of the electrospun saccharide fibre was observed to follow that typical of electrospun polymers. Based on initial food grade glucose syrup tests, various solutions based on combinations of syrup components, i.e. mono-, di- and tri-saccharides, were investigated to map out materials and electrospinning conditions thatwould lead to the formation of fibre. Thiswork demonstrated that sucrose exhibits the highest propensity for fibre formation during electrospinning amongst the various types of saccharide solutions studied. The possibility of electrospinning low molecular weight saccharides into sub-micron fibres has implications for the electrospinability of supramolecular polymers and other biomaterials.","PeriodicalId":92629,"journal":{"name":"Electrospinning","volume":"1 1","pages":"1 - 9"},"PeriodicalIF":0.0,"publicationDate":"2015-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1515/esp-2016-0001","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67352054","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

Fabrication and characterization of air-impedance electrospun polydioxanone templates 空气阻抗静电纺聚二氧环酮模板的制备与表征

Electrospinning Pub Date : 2015-04-26 DOI: 10.1515/esp-2016-0003

Gretchen S. Selders, A. Fetz, Shannon L. Speer, G. Bowlin

{"title":"Fabrication and characterization of air-impedance electrospun polydioxanone templates","authors":"Gretchen S. Selders, A. Fetz, Shannon L. Speer, G. Bowlin","doi":"10.1515/esp-2016-0003","DOIUrl":"https://doi.org/10.1515/esp-2016-0003","url":null,"abstract":"Abstract Electrospinning, a fabrication technique used to create non-woven, porous templates from natural and synthetic polymers, is commonly used in tissue engineering because it is highly tailorable. However, traditional electrospinning creates restrictive pore sizes that limit the required cell migration. Therefore, tissue engineering groups have sought to enhance and regulate porosity of tissue engineering templates. Air-impedance electrospinning generates templates with tailorable, patterned areas of low and high density fiber deposition. Here we demonstrate an improved air-impedance electrospinning system, consisting of a newly designed funnel equipped to hold changeable porous deposition plates and administer air flow in a confined and focused manner, with parameters that maintain template integrity. In this preliminary study, we quantify the increase in porosity of polydioxanone templates with use of traditional fiber and pore analysis as well as with mercury porosimetry. Additionally, we validate the system’s significance in fabricating enhanced porosity templates that maintain their mechanical properties (i.e. elastic modulus, tensile strength, and suture retention strength) despite the deliberate increase in porosity. This is of exceptional value to the template’s integrity and efficacy as these parameters can be further optimized to induce the desired template porosity, strength, and texture for a given application.","PeriodicalId":92629,"journal":{"name":"Electrospinning","volume":"1 1","pages":"20 - 30"},"PeriodicalIF":0.0,"publicationDate":"2015-04-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1515/esp-2016-0003","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67352190","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6

首页上一页