British journal of experimental pathology最新文献_第2页

Amelioration of established Sendai viral pneumonia in the nude mouse using a monoclonal antibody to the virus fusion protein. 利用病毒融合蛋白单克隆抗体改善裸鼠仙台病毒性肺炎。

British journal of experimental pathology Pub Date : 1989-12-01

P Carthew, J Riley, D Dinsdale

引用次数: 0

Enhancement of inflammatory reactions in a non-immunological air pouch model in rats. 大鼠非免疫气囊模型炎症反应增强。

British journal of experimental pathology Pub Date : 1989-12-01

M Isaji, Y Momose, J Naito

{"title":"Enhancement of inflammatory reactions in a non-immunological air pouch model in rats.","authors":"M Isaji, Y Momose, J Naito","doi":"","DOIUrl":"","url":null,"abstract":"In a carboxymethyl cellulose (CMC) air pouch inflammation model, accumulation of exudate decreased at a relatively rapid rate and almost disappeared 3 days after a 2% CMC injection into the preformed air pouch. After a second injection of 2% CMC solution into the 1-day-old CMC pouch on the day following the first CMC injection, the decrease in rate of exudate was similar to the change seen after the first CMC injection. In another group of rats, 3 days after the first CMC injection when inflammation had subsided, a second injection of 2% CMC solution into the 3-day-old CMC pouch resulted in a marked increase of exudate accumulation, inflammatory cell infiltration and vascular permeability. Histologically, large numbers of macrophages accumulated in the 3-day-old CMC pouch and fibroblast proliferation and newly formed blood vessels were also visible. The enhanced exudative reaction was significantly inhibited by dexamethasone but not by indomethacin. These results indicate that the enhanced inflammatory reactions appear to be closely correlated with the increase of reactivity at the site of inflammation and the exudative reaction was not mediated by cyclo-oxygenase products.","PeriodicalId":9248,"journal":{"name":"British journal of experimental pathology","volume":"70 6","pages":"705-16"},"PeriodicalIF":0.0,"publicationDate":"1989-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2040720/pdf/brjexppathol00150-0105.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13746820","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biosynthesis of interleukin-I beta at inflammatory site in rabbits: kinetics and producing cells. 兔炎症部位白细胞介素- 1 β的生物合成:动力学和生成细胞。

British journal of experimental pathology Pub Date : 1989-12-01

F Goto, K Goto, S Mori, S Ohkawara, M Yoshinaga

引用次数: 0

Quantitative evaluation of myocardial injury induced by acute cerebral ischaemia and its prevention by beta 1-adrenergic blockade. An ultrastructural morphometry study. 急性脑缺血心肌损伤的定量评价及β 1-肾上腺素能阻断剂的预防作用。超微结构形态学研究。

British journal of experimental pathology Pub Date : 1989-12-01

A Kolin, A Brezina, A J Lewis, J W Norris

{"title":"Quantitative evaluation of myocardial injury induced by acute cerebral ischaemia and its prevention by beta 1-adrenergic blockade. An ultrastructural morphometry study.","authors":"A Kolin, A Brezina, A J Lewis, J W Norris","doi":"","DOIUrl":"","url":null,"abstract":"The protective effects of beta 1-adrenergic blockade with metoprolol (Betaloc Astra) were demonstrated in the gerbil model of myocardial injury provoked by acute ischaemic brain lesions. The myocardial injury was reversible and lipid droplet accumulation was its most striking morphological feature. These droplets were easy to measure in EM photographs and their size was expressed as percentage of sarcoplasmic volume. The EM data of fat accumulation were compared in hearts of carotid-ligated animals with and without metoprolol pretreatment, and in animals with the carotid isolated only, at standard intervals 3-48 h after operation. While in carotid-ligated-only animals the average myocardial fat contents rose to a peak of 1.9% at 10 h, in metoprolol pretreated animals the amount of fat was always significantly lower and started to return earlier to basal values (peak at 6 h, 1.1%). In carotid-isolated-only animals, fat accumulation peaked at 6-10 h (1.1%) and returned quickly to normal levels (0.34 +/- 0.18%). This effective pharmacological blockade with metoprolol strongly supports the concept of catecholamine mediation between acute intracranial lesions and myocardial injury. The background and significance of myocardial fat accumulation is discussed. The EM morphometry of fat droplets appears to be a suitable tool for quantification of reversible myocardial damage most useful for experimental evaluation of cardioprotective measures. As changes in succinic dehydrogenase histochemistry (from 'myofibrillar' to 'granular' pattern) correlated with EM measured fat accumulation, the simplicity and speed of the SDH method recommends itself for fast orientation about presence of myocardial damage.","PeriodicalId":9248,"journal":{"name":"British journal of experimental pathology","volume":"70 6","pages":"659-67"},"PeriodicalIF":0.0,"publicationDate":"1989-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2040714/pdf/brjexppathol00150-0063.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13746816","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Heterogeneity of hepatocyte antigen expression in rat liver carcinogenesis: concordance between neoplastic nodules and tumours. 大鼠肝癌发生过程中肝细胞抗原表达的异质性:肿瘤结节与肿瘤的一致性。

British journal of experimental pathology Pub Date : 1989-12-01

M J Embleton, H S James, A J Haynes, P C Butler

{"title":"Heterogeneity of hepatocyte antigen expression in rat liver carcinogenesis: concordance between neoplastic nodules and tumours.","authors":"M J Embleton, H S James, A J Haynes, P C Butler","doi":"","DOIUrl":"","url":null,"abstract":"Fischer F344 rats were given a cyclical diet of 0.06% 2-acetylaminofluorene (AAF), which progressively induced oval cell proliferation, cirrhosis and hyperplastic (or neoplastic) nodules. Primary liver tumours developed from 7 months after ceasing the diet. Liver samples taken during and after AAF administration and specimens of primary tumours were processed into frozen sections and examined microscopically for morphological changes in cell populations, stained histochemically for gamma-glutamyl transpeptidase (GGTase) and four phosphatases, and stained by the immunoperoxidase technique for the presence of antigens detected by seven anti-liver cell monoclonal antibodies and monoclonal antibodies to six oncoproteins. During and after AAF treatment several of the anti-liver antibodies revealed foci of aberrantly or heterogeneously-stained cells, although anti-oncoprotein antibodies showed no consistent changes. Foci of cells positive for GGTase and heterogeneous for adenosine triphosphatase (ATPase) were also seen. Nodules invariably showed heterogeneous antigenicity, raised GGTase and abnormal ATPase expression. Primary tumours exhibited varying degrees of positivity, negativity and heterogeneity with the anti-liver monoclonal antibodies, and all were positive for GGTase. Comparison between various parameters and different lesions showed the greatest concordance between nodules and tumours, suggesting that nodules are probably the precursors of malignant tumours in this system.","PeriodicalId":9248,"journal":{"name":"British journal of experimental pathology","volume":"70 6","pages":"647-57"},"PeriodicalIF":0.0,"publicationDate":"1989-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2040721/pdf/brjexppathol00150-0052.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13676361","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Immunoregulation in Heymann nephritis. II. Functional studies. 海曼肾炎的免疫调节。2功能的研究。

British journal of experimental pathology Pub Date : 1989-10-01

J Cornish, A Z Barabas, R Lannigan, J Rozing

{"title":"Immunoregulation in Heymann nephritis. II. Functional studies.","authors":"J Cornish, A Z Barabas, R Lannigan, J Rozing","doi":"","DOIUrl":"","url":null,"abstract":"In this study, the functional properties of the cells involved in the immunoregulation of Heymann nephritis (HN) have been investigated. HN is a disease model in the rat where the pathology closely resembles membranous glomerulonephropathy (MGN) in man. This autoimmune model is induced by injection of renal tubular antigen (RTA) incorporated in Freund's complete adjuvant (FCA). The strong B cell and plasma cell response in the chronic phase of HN, as determined by cell marker analyses, is predominantly antigen-non-specific. The secondary response pattern found was not only to RTA upon repeated immunization, but also to non-related antigen (SRBC). Although cell marker studies have indicated no major quantitative changes in the T cell population throughout the development of HN, a severe deregulation of the cellular immune response is observed especially during the induction period of HN. This was shown by a strong decrease of the mitogen-induced proliferative response and IL-2 production. This phenomenon is caused by both defective cellular components and inhibitory serological factors. Finally, in the chronic phase, these aberrations gradually return to normal.","PeriodicalId":9248,"journal":{"name":"British journal of experimental pathology","volume":"70 5","pages":"505-13"},"PeriodicalIF":0.0,"publicationDate":"1989-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2040602/pdf/brjexppathol00149-0015.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13956227","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Immunoregulation in Heymann nephritis. I. Cell marker studies. 海曼肾炎的免疫调节。1 .细胞标记研究。

British journal of experimental pathology Pub Date : 1989-10-01

J Cornish, A Z Barabas, R Lannigan, J Rozing

引用次数: 0

Localization of antiglomerular basement membrane-binding polyclonal antibodies in rat lung and kidney using post-embedding immunogold electron microscopy. 应用包埋后免疫金电镜定位大鼠肺、肾抗肾小球基底膜结合多克隆抗体。

British journal of experimental pathology Pub Date : 1989-10-01

N S Fernando, N W Boyce, S R Holdsworth, T J Neale

引用次数: 0

Membrane N-acetylglucosamine: expression by cells in rheumatoid synovial fluid, and by pre-cultured monocytes. 膜n -乙酰氨基葡萄糖:类风湿性滑膜液细胞和预培养单核细胞表达。

British journal of experimental pathology Pub Date : 1989-10-01

M Sharif, L S Wilkinson, J Edwards, G A Rook

{"title":"Membrane N-acetylglucosamine: expression by cells in rheumatoid synovial fluid, and by pre-cultured monocytes.","authors":"M Sharif, L S Wilkinson, J Edwards, G A Rook","doi":"","DOIUrl":"","url":null,"abstract":"After 21-48 h in culture, 2-8% of human peripheral blood monocytes strongly express terminal N-acetylglucosamine (GlcNAc) on their membranes. This can be detected with a monoclonal antibody selected for binding to asialo-agalacto-fetuin, and is eliminated by incubating the cells in pure N-acetylglucosaminidase. Expression of GlcNAc is transient, and can no longer be detected by day 4. These cells are a subset of macrophages since they are positive for non-specific esterase and stained by the monoclonal antibody EBM 11. GlcNAc-positive cells showing double staining with monoclonal antibodies UCHM1 and RFD7 were detected. Their numbers were not influenced by the addition of GM-CSF, IFN-gamma, 1,25-(OH)2 cholecalciferol or indomethacin. Macrophages which give membrane staining for terminal GlcNAc were also found in rheumatoid synovial fluid, and in synovial tissue, though in the peripheral blood their frequency was the same in samples from normal donors and from patients with rheumatoid arthritis. Immunoblots of 24-48-h monocyte cultures or of fresh synovial fluid cells using the anti-GlcNAc monoclonal, show the anticipated agalactosyl IgG heavy chains, and an additional band of 70-80kDa.","PeriodicalId":9248,"journal":{"name":"British journal of experimental pathology","volume":"70 5","pages":"567-77"},"PeriodicalIF":0.0,"publicationDate":"1989-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2040600/pdf/brjexppathol00149-0072.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13824912","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Prostanoid release by macrophages at a distance from an inflammatory site. 巨噬细胞在远离炎症部位的地方释放前列腺素。

British journal of experimental pathology Pub Date : 1989-10-01

M Tissot, S Strzalko, A Thuret, J P Giroud

引用次数: 0