Brazilian Journal of Pharmaceutical Sciences最新文献_第2页

Advancements in artificial intelligence and machine learning in revolutionising biomarker discovery 人工智能和机器学习在彻底改变生物标志物发现方面的进展

IF 1.3 4区医学

Brazilian Journal of Pharmaceutical Sciences Pub Date : 2023-08-28 DOI: 10.1590/s2175-97902023e23146

G. S. Raikar, A. S. Raikar, S. Somnache

引用次数: 0

Effect of polycaprolactone nanocapsules loaded with essential oils on biofilm formation by Staphylococcus aureus strains isolated from bovine mastitis cases 精油负载聚己内酯纳米胶囊对牛乳腺炎金黄色葡萄球菌生物膜形成的影响

IF 1.3 4区医学

Brazilian Journal of Pharmaceutical Sciences Pub Date : 2023-08-28 DOI: 10.1590/s2175-97902023e23068

Gabriela Suthovski, Alcione Santa Catarina, Diana Paula Perin, R. Mainardes, Karina Ramirez Starikoff, A. Gallina, Maiara Garcia Blagitz Azevedo, F. Dalmolin, Luciana Velasques Cervo, D. M. Benvegnú

{"title":"Effect of polycaprolactone nanocapsules loaded with essential oils on biofilm formation by Staphylococcus aureus strains isolated from bovine mastitis cases","authors":"Gabriela Suthovski, Alcione Santa Catarina, Diana Paula Perin, R. Mainardes, Karina Ramirez Starikoff, A. Gallina, Maiara Garcia Blagitz Azevedo, F. Dalmolin, Luciana Velasques Cervo, D. M. Benvegnú","doi":"10.1590/s2175-97902023e23068","DOIUrl":"https://doi.org/10.1590/s2175-97902023e23068","url":null,"abstract":"Bovine infectious mastitis is largely resistant to antibacterial treatment, mainly due to mechanisms of bacterial resistance in the biofilms formed by Staphylococcus aureus. Melaleuca (MEO) and citronella essential oils (CEO) are promising agents for reducing or eliminating biofilms. Free melaleuca oil presented a medium Minimum Inhibitory Concentration (MIC) of 0.625% and a Minimum Bactericidal Concentration (MBC) of 1.250%, while free citronella oil showed medium MIC and MBC of 0.313%. Thus, free CEO and MEO demonstrate bacteriostatic and bactericidal potential. We generated polymeric nanocapsules containing MEO or CEO and evaluated their efficacy at reducing biofilms formed by S. aureus . Glass and polypropylene spheres were used as test surfaces. To compare the responses of free and encapsulated oils, strains were submitted to 10 different procedures, using free and nanoencapsulated essential oils (EOs) in vitro . We observed no biofilm reduction by MEO, free or nanoencapsulated. However, CEO nanocapsules reduced biofilm formation on glass (p = 0.03) and showed a tendency to diminish biofilms on polypropylene (p = 0.051). Despite nanoencapsulated CEO reducing biofilms in vitro , the formulation could be improved to modify the CEO component polarity and, including MEO, to obtain more interactions with surfaces and the biofilm matrix.","PeriodicalId":9218,"journal":{"name":"Brazilian Journal of Pharmaceutical Sciences","volume":"1 1","pages":""},"PeriodicalIF":1.3,"publicationDate":"2023-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67743188","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Swertiamarin attenuates paraquat-induced pulmonary epithelial-like cell apoptosis via NOX4-mediated regulation of redox and mitochondrial function Swertiamarin通过NOX4介导的氧化还原和线粒体功能调节减轻百草枯诱导的肺上皮样细胞凋亡

IF 1.3 4区医学

Brazilian Journal of Pharmaceutical Sciences Pub Date : 2023-08-28 DOI: 10.1590/s2175-97902023e22476

Ji Wu, Yong Li, Xinju Li, Tao Wu

{"title":"Swertiamarin attenuates paraquat-induced pulmonary epithelial-like cell apoptosis via NOX4-mediated regulation of redox and mitochondrial function","authors":"Ji Wu, Yong Li, Xinju Li, Tao Wu","doi":"10.1590/s2175-97902023e22476","DOIUrl":"https://doi.org/10.1590/s2175-97902023e22476","url":null,"abstract":"The aim of the present study was to investigate the effect of swertiamarin (STM) in attenuating paraquat (PQ)-induced human lung alveolar epithelial-like cell (A549) apoptosis and the underlying mechanisms. A549 cells were pretreated with different concentrations of STM for 2 hr and then cultured with or without PQ (700 μM) for 24 hr. Cell survival was determined using the CCK8 assay. Morphological changes, MDA content, inflammatory factors, fibrogenesis parameters, apoptosis rates, redox status and mitochondrial membrane potential (MMP) were evaluated. The expression of several genes involved in the modulation of redox status was measured by Western blotting. Cell viability and MMP were decreased, but the apoptosis rate and DCFH oxidation were elevated by PQ exposure. STM pretreatment notably increased cell viability and MMP and reduced the apoptosis rate and DCFH oxidation. Furthermore, TLR4-NOX4 signaling was significantly inhibited by STM. The downregulation of NOX4 by siRNA exerted the same protective effects as STM. This study provides the first evidence that STM attenuates PQ-induced pulmonary epithelial-like cell apoptosis via NOX4-mediated regulation of redox and mitochondrial function.","PeriodicalId":9218,"journal":{"name":"Brazilian Journal of Pharmaceutical Sciences","volume":"1 1","pages":""},"PeriodicalIF":1.3,"publicationDate":"2023-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67741942","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Research on Anti-inflammatory Targets and Mechanisms of alkaloids in Picrasma quassioides Benn Through Network Pharmacology 网络药理学研究拟甾体坏死病生物碱抗炎作用靶点及机制

IF 1.3 4区医学

Brazilian Journal of Pharmaceutical Sciences Pub Date : 2023-08-28 DOI: 10.1590/s2175-97902023e22394

Qijia Xu, Kai Wang, Yaoyao Xu, Yinhe Gao, Ge Wang, Sheng Liu, Feng Zhao

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Long-Term Care medicines formularies: any reasons for pharmacists’ concern? 长期护理药物处方:药剂师担心的原因是什么?

IF 1.3 4区医学

Brazilian Journal of Pharmaceutical Sciences Pub Date : 2023-08-28 DOI: 10.1590/s2175-97902023e22802

J. R. Gonçalves, Giordano Avancini Sole, A. Cavaco

{"title":"Long-Term Care medicines formularies: any reasons for pharmacists’ concern?","authors":"J. R. Gonçalves, Giordano Avancini Sole, A. Cavaco","doi":"10.1590/s2175-97902023e22802","DOIUrl":"https://doi.org/10.1590/s2175-97902023e22802","url":null,"abstract":"This study aimed to characterize and compare medicines formularies (MFs) used in Long-Term Care (LTC) facilities in Portugal, and to identify the prevalence of Potentially Inappropriate Medicines (PIMs). A systematic contact with LTC facilities was undertaken in December 2021. MFs were systematized according to the Anatomical Therapeutical Chemical classification system (ATC), followed by descriptive content analysis. A structured comparison between MFs developed by public organizations and private LTC facilities was performed. After duplicate removal and exclusion of medicines not for systemic use, two explicit criteria - the Algorithm of medication review in frail older people and the EU(7)-PIM list - were employed for PIMs identification. Five MFs were obtained and assessed. The three MFs developed by private institutions covered 23% of the national LTC facilities and approximately 34% of the national total of beds. Heterogeneity was particularly high for the Alimentary tract and metabolism , Blood and blood-forming organs , Musculoskeletal system , and Respiratory system ATC groups. A PIM prevalence of 29,4% was identified. Medicines distribution between the MFs suggests the need to develop national guidelines towards harmonizing medicines usage in LTC. The prevalence of PIMs found highlights the importance of a particular optimized use of this health technology in aged sub-populations.","PeriodicalId":9218,"journal":{"name":"Brazilian Journal of Pharmaceutical Sciences","volume":"1 1","pages":""},"PeriodicalIF":1.3,"publicationDate":"2023-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67743300","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mechanical properties and immunotherapeutic effects of dissolving microneedles with different drug loadings based on hyaluronic acid 基于透明质酸的不同载药量溶解微针的力学性能和免疫治疗效果

IF 1.3 4区医学

Brazilian Journal of Pharmaceutical Sciences Pub Date : 2023-08-28 DOI: 10.1590/s2175-97902023e22690

Dazhi Wang, Meihua Jiang, Xiaowen Wang, Chen Wang, Xiaoyu Ou, Lei Shang

{"title":"Mechanical properties and immunotherapeutic effects of dissolving microneedles with different drug loadings based on hyaluronic acid","authors":"Dazhi Wang, Meihua Jiang, Xiaowen Wang, Chen Wang, Xiaoyu Ou, Lei Shang","doi":"10.1590/s2175-97902023e22690","DOIUrl":"https://doi.org/10.1590/s2175-97902023e22690","url":null,"abstract":". Improving vaccine immunity and reducing antigen usage are major challenges in the clinical application of vaccines. Microneedles have been proven to be painless, minimally invasive, highly efficient, and have good patient compliance. Compared with traditional transdermal drug delivery, it can effectively deliver a large-molecular-weight drug into the skin, resulting in a corresponding immune response. However, few studies have examined the relationship between microneedle loading dose and immune effects. In this study, the hyaluronic acid (HA) conical and pyramidal dissolving microneedles were prepared by the two-step vacuum drying method, respectively. The model drug ovalbumin (OVA) was added to HA to prepare dissolving microneedles with different loading amounts. The mass ratios of HA to OVA were 5:1, 5:3, and 5:5. The mechanical properties of the dissolving microneedles were characterized using nanoindentation and in vitro puncture studies. The immune effects of the matrix and drug content were studied in Sprague–Dawley (SD) rats. Finally, the diffusion behavior of OVA and the binding mode of HA and OVA in the microneedles were simulated using Materials Studio and Autodocking software. The experimental results showed that the conical microneedles exhibited better mechanical properties. When the mass ratio of HA to OVA was 5:3, the immune effect can be improved by 37.01% compared to subcutaneous injection, and achieved a better immune effect with relatively fewer drugs. This conclusion is consistent with molecular simulations. This study provides theoretical and experimental support for the drug loading and efficacy of microneedles with different drug loadings.","PeriodicalId":9218,"journal":{"name":"Brazilian Journal of Pharmaceutical Sciences","volume":"1 1","pages":""},"PeriodicalIF":1.3,"publicationDate":"2023-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67742370","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Existence of advanced glycation / lipoxidation end-products in parenteral nutrition solutions and effects of infusion conditions on their levels 肠外营养液中晚期糖基化/脂氧化终产物的存在及其输注条件对其水平的影响

IF 1.3 4区医学

Brazilian Journal of Pharmaceutical Sciences Pub Date : 2023-08-28 DOI: 10.1590/s2175-97902023e23017

N. Kahramansoy, M. Çelebier

{"title":"Existence of advanced glycation / lipoxidation end-products in parenteral nutrition solutions and effects of infusion conditions on their levels","authors":"N. Kahramansoy, M. Çelebier","doi":"10.1590/s2175-97902023e23017","DOIUrl":"https://doi.org/10.1590/s2175-97902023e23017","url":null,"abstract":"Infusion solutions must be stable from the production stage until the infusion stage. Some infusion fluids contain degradation products, known as advanced glycation end products (AGEs); however, it is unknown whether AGEs exist in parenteral nutrition solutions. We aimed to investigate this question and test the effect of infusion conditions on AGE formation in parenteral nutrition solution. Nine parenteral nutrition solutions were supplied by the pharmacy with which we collaborated. To simulate the infusion conditions, the solutions were held in a patient room with standard lighting and temperature for 24 hours. Samples were taken at the beginning (group A) and the end (24th hour, group B) of the infusion period. The degradation products were 3-deoxyglucosone, pentosidine, N-carboxymethyl lysine, and 4-hydroxynonenal, which we investigated by high-performance liquid chromatography-mass spectrometry (LC-MS) and Q-TOF LC/MS methods. Two of four degradation products, 4-hydroxynonenal and N-carboxymethyl lysine, were detected in all samples, and Group B had higher levels of both compounds compared to Group A, who showed that the quantities of these compounds increased in room conditions over time. The increase was significant for 4-hydroxynonenal (p=0.03), but not for N-carboxymethyl lysine (p=0.23). Moreover, we detected in the parenteral nutrition solutions a compound that could have been 4-hydroxy-2-butynal or furanone.","PeriodicalId":9218,"journal":{"name":"Brazilian Journal of Pharmaceutical Sciences","volume":"1 1","pages":""},"PeriodicalIF":1.3,"publicationDate":"2023-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67742947","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Egr-1 Enhances Drug Resistance of Breast Cancer Cells by MDR1 Dependence Egr-1通过MDR1依赖性增强乳腺癌细胞耐药

IF 1.3 4区医学

Brazilian Journal of Pharmaceutical Sciences Pub Date : 2023-08-28 DOI: 10.1590/s2175-97902023e18705

P. Gong

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Development and characterization of photoprotective nanoemulsions containing Babassu (Orbignya phalerata Mart.) lipophilic extract 含巴巴苏(Orbignya phalerata Mart.)亲脂提取物的光防护纳米乳液的研制与表征

IF 1.3 4区医学

Brazilian Journal of Pharmaceutical Sciences Pub Date : 2023-08-28 DOI: 10.1590/s2175-97902023e23011

I. Vieira, Ana Clara Lima Nunes Silva, N. Castro, Cristal S C Pinto, Z. Freitas, E. Ricci-Júnior, E. P. Santos, A. L. Camara, M. C. P. Costa, Carlos Adam Conte-Junior

{"title":"Development and characterization of photoprotective nanoemulsions containing Babassu (Orbignya phalerata Mart.) lipophilic extract","authors":"I. Vieira, Ana Clara Lima Nunes Silva, N. Castro, Cristal S C Pinto, Z. Freitas, E. Ricci-Júnior, E. P. Santos, A. L. Camara, M. C. P. Costa, Carlos Adam Conte-Junior","doi":"10.1590/s2175-97902023e23011","DOIUrl":"https://doi.org/10.1590/s2175-97902023e23011","url":null,"abstract":"Oil-in-water photoprotective nanoemulsions (NEs) were developed using Babassu (BBS) lipophilic extract, nonionic surfactants, and low concentrations of organic sunscreens by ultrasonic processing. BBS extract was chosen due to its suitable physicochemical properties (acidity index, peroxide index, refraction index, and relative density) and predominance of saturated fatty acids, identified by gas chromatography-mass spectrometry (GC-MS), which promote biological activities and high oxidative stability. NEs were characterized by mean droplet size, morphology, polydispersity index (PdI), pH, and organoleptic properties, and the physical stability of the NEs was evaluated for 120 days at room temperature. The sun protection factor (SPF) was determined, and the photostability and in vitro cytotoxicity assays were performed for NEs. All NEs remained stable for 120 days, with a droplet size <150 nm and a monomodal distribution profile. The pH values were compatible with the skin’s pH. NE3 showed a spherical morphology, with a mean droplet size of 125.15 ± 0.16 nm and PdI of 0.145 ± 0.032. NE3 containing BBS extract and sunscreens presented an SPF of 35.5 ± 3.0, was photostable after 6 h of radiation and was non-cytotoxic to fibroblast cells. Thus, NE3 could be considered a promising formulation for developing synergic plant-extract sunscreen photoprotective products for the market.","PeriodicalId":9218,"journal":{"name":"Brazilian Journal of Pharmaceutical Sciences","volume":"1 1","pages":""},"PeriodicalIF":1.3,"publicationDate":"2023-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67743420","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Doxorubicin cardiotoxicity and Camellia sinensis cardioprotection determined by speckle-tracking echocardiography 斑点跟踪超声心动图测定阿霉素的心脏毒性和对中华山茶的心脏保护作用

IF 1.3 4区医学

Brazilian Journal of Pharmaceutical Sciences Pub Date : 2023-08-28 DOI: 10.1590/s2175-97902023e23063

M. Barreto, J. L. Carvalho, M. Melo, A. Michel, M. G. Ferreira, Ruthnéa Aparecida Lázaro Muzzi, A. Goes, M. M. Melo

{"title":"Doxorubicin cardiotoxicity and Camellia sinensis cardioprotection determined by speckle-tracking echocardiography","authors":"M. Barreto, J. L. Carvalho, M. Melo, A. Michel, M. G. Ferreira, Ruthnéa Aparecida Lázaro Muzzi, A. Goes, M. M. Melo","doi":"10.1590/s2175-97902023e23063","DOIUrl":"https://doi.org/10.1590/s2175-97902023e23063","url":null,"abstract":"Doxorubicin (Dox) is a medication used in the treatment of cancerous tumors and hematologic malignancies with potentially serious side effects, including the risk of cardiotoxicity. Flavonoids are plant metabolites with antioxidant properties and can be extracted from Camellia sinensis (CS). The aim of this study is to evaluate the possible cardioprotective effect of CS against injuries induced by Dox in rats. A total of 32 animals were distributed into four groups: (1) control - intraperitoneal injection (I.P.) of 0.5 mL saline weekly and 1.0 mL water by gavage daily; (2) CS - 0.5 mL saline I.P. weekly and 200 mg/kg CS by gavage daily; (3) Dox - 5.0 mg/kg Dox I.P. weekly and 1.0 mL water by gavage daily; and (4) Dox + CS -5.0 mg/kg Dox I.P. weekly and 200 mg/kg CS by gavage daily. Clinical examinations, blood profiles, electrocardiograms, echocardiograms, and histological analyses of hearts were performed over 25 days. The animals in the Dox group showed changes in body weight and in erythrogram, leukogram, electrocardiography, and echocardiography readings. However, animals from the dox+CS group had significantly less change in body weight, improved cardiac function, and showed more preserved cardiac tissue. This study demonstrated that CS prevents dox-induced cardiotoxicity, despite enhancing the cytotoxic effect on blood cells.","PeriodicalId":9218,"journal":{"name":"Brazilian Journal of Pharmaceutical Sciences","volume":"1 1","pages":""},"PeriodicalIF":1.3,"publicationDate":"2023-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67743072","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0