Brazilian Journal of Pharmaceutical Sciences最新文献_第2页

Moderate Toxicity of Potential Boron-containing Therapeutic, Dipotassium-trioxohydroxytetrafl uorotriborate -K2(B3O3F4OH) in Rats and Mice 潜在的含硼治疗药物二钾-三氧羟基四氟三硼酸钾-K2(B3O3F4OH)对大鼠和小鼠的中度毒性

IF 1.3 4区医学

Brazilian Journal of Pharmaceutical Sciences Pub Date : 2023-08-28 DOI: 10.1590/s2175-97902023e21384

A. Haverić, S. Haverić, M. Hadzic, J. Ezić, T. Cetkovic, B. Galić

{"title":"Moderate Toxicity of Potential Boron-containing Therapeutic, Dipotassium-trioxohydroxytetrafl uorotriborate -K2(B3O3F4OH) in Rats and Mice","authors":"A. Haverić, S. Haverić, M. Hadzic, J. Ezić, T. Cetkovic, B. Galić","doi":"10.1590/s2175-97902023e21384","DOIUrl":"https://doi.org/10.1590/s2175-97902023e21384","url":null,"abstract":"Biological activity of boron-containing compounds (BCCs) has been well-known. Growing interest and numerous applications for BCCs have been reported. Boron and boron-containing acids show low acute toxicity in mammals but data on halogenated boroxine (HB) - dipotassium-trioxohydroxytetrafluorotriborate, K 2 (B 3 O 3 F 4 OH) acute toxicity have not been reported before. This compound, characterized as a potential therapeutic for skin changes, exhibits no observable genotoxicity in doses lower that 0.1 mg/ml in vitro and 55 mg/kg in vivo. It has also been confirmed as an antitumour agent both in vitro and in vivo as well as an inhibitor of enzymes involved in antioxidant mechanisms. The aim of this study was to assess the acute toxicity of HB and to determine the maximum tolerated dose as well as a dose free of any signs of toxicity in different test organisms. Acute toxicity of HB was tested in Sprague-Dawley and Wistar rats and BALB/c mice after single parenteral application of different doses. We determined doses free of any sign of toxicity and LD 50 after single dose administration. LD 50 of HB ranges from 63 to 75 mg/kg in different test models, meaning that HB shows moderate toxicity.","PeriodicalId":9218,"journal":{"name":"Brazilian Journal of Pharmaceutical Sciences","volume":null,"pages":null},"PeriodicalIF":1.3,"publicationDate":"2023-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67739499","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

BALB/c and C57BL/6 mouse strains influence gastric function outcomes with administration of cisplatin and dexamethasone 小鼠BALB/c和C57BL/6菌株对顺铂和地塞米松给药后胃功能的影响

IF 1.3 4区医学

Brazilian Journal of Pharmaceutical Sciences Pub Date : 2023-08-28 DOI: 10.1590/s2175-97902023e22718

L. A. Gama, Mariana Pirani Rocha Machado, L. Corá, A. P. S. Beckmann, Wellington David Luz Alves, J. Miranda, M. Américo

{"title":"BALB/c and C57BL/6 mouse strains influence gastric function outcomes with administration of cisplatin and dexamethasone","authors":"L. A. Gama, Mariana Pirani Rocha Machado, L. Corá, A. P. S. Beckmann, Wellington David Luz Alves, J. Miranda, M. Américo","doi":"10.1590/s2175-97902023e22718","DOIUrl":"https://doi.org/10.1590/s2175-97902023e22718","url":null,"abstract":"Our aim was to evaluate the effects of cisplatin and dexamethasone alone and combined on gastric contractility and histomorphometry of BALB/c and C57BL/6 mice. BALB/c and C57BL/6 male mice (8-week-old) were randomly separated into: Control; Cisplatin (7.5 mg/Kg); Dexamethasone (2.0 mg/Kg); and Dexamethasone plus Cisplatin (2.0 mg/Kg of dexamethasone 1-hour prior to 7.5 mg/Kg of cisplatin). Drugs were administered intraperitoneally for three days. Body weight and food intake were evaluated on 2nd day. Alternating Current Biosusceptometry technique was employed to measure gastric contractions on 3rd day. Afterward, mice were killed for gastric histomorphometric analysis. Cisplatin decreased food intake and caused bradygastria in BALB/c mice; however, the amplitude of gastric contractions decreased in both BALB/c and C57BL/6. Dexamethasone and cisplatin combined restored the gastric frequency and food intake only in BALB/c, but drug combination reduced the gastric amplitude of contractions in both strains. Dexamethasone alone increased gastric mucosa thickness in C57BL/6 and decreased muscular thickness in BALB/c. In conclusion, the mouse strains presented differences in acute effects of cisplatin and dexamethasone alone and combined on gastric function. This reinforces the importance of choosing the appropriate mouse strain for studying the acute effects of drugs on the gastrointestinal tract.","PeriodicalId":9218,"journal":{"name":"Brazilian Journal of Pharmaceutical Sciences","volume":null,"pages":null},"PeriodicalIF":1.3,"publicationDate":"2023-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67742530","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Authentication of Brazilian Ginseng using Bar-HRM analysis Bar-HRM法鉴定巴西人参

IF 1.3 4区医学

Brazilian Journal of Pharmaceutical Sciences Pub Date : 2023-08-28 DOI: 10.1590/s2175-97902023e21179

Francine Alves Nogueira de Almeida, P. V. Oliveira, Natan Silva Matos, Verônica Luiza Silveira Fialho, M. D. Lugon, M. C. Vieira, M. F. S. Ferreira, G. G. Paneto

{"title":"Authentication of Brazilian Ginseng using Bar-HRM analysis","authors":"Francine Alves Nogueira de Almeida, P. V. Oliveira, Natan Silva Matos, Verônica Luiza Silveira Fialho, M. D. Lugon, M. C. Vieira, M. F. S. Ferreira, G. G. Paneto","doi":"10.1590/s2175-97902023e21179","DOIUrl":"https://doi.org/10.1590/s2175-97902023e21179","url":null,"abstract":"Hebanthe eriantha (Martius) Kuntze and Pfaffia glomerata (Spreng) Pedersen are medicinal plants popularly known as “Brazilian Ginseng” due to their similarity to Panax ginseng . In Brazil, they are sold as the same herb, despite their different pharmacological and toxicological properties. The morphological identification is difficult, which facilitates their adulteration. We report the application of the Barcode DNA High-Resolution Melting (Bar-HRM) using matK gene to differentiate both species in samples sold in the Brazilian market. Using the proposed method, we could discriminate and identify both species. Bar-HRM analysis allowed discriminating and identifying both species. It allowed the identification of H. eriantha and P. glomerata in 43.6% and 56.4% of the amplified samples, respectively. Of these, only seven samples were authenticated and, in 71.4% of the cases, adulterated. We concluded that Bar-HRM has proven to be a fast alternative method to authenticate plants under the common name “Brazilian Ginseng”.","PeriodicalId":9218,"journal":{"name":"Brazilian Journal of Pharmaceutical Sciences","volume":null,"pages":null},"PeriodicalIF":1.3,"publicationDate":"2023-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67739118","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Experimental acute anti-inflammatory activity of preparations with complexed cannabidiol in carriers 复合大麻二酚载体制剂的实验性急性抗炎活性

IF 1.3 4区医学

Brazilian Journal of Pharmaceutical Sciences Pub Date : 2023-08-28 DOI: 10.1590/s2175-97902023e221000

Daniel Ribeiro Grijó, Edvalkia Magna Teobaldo da Rocha, Vitor de Cinque Almeida, C. Amado, J. E. Olivo, Oswaldo Curty da Motta Lima

{"title":"Experimental acute anti-inflammatory activity of preparations with complexed cannabidiol in carriers","authors":"Daniel Ribeiro Grijó, Edvalkia Magna Teobaldo da Rocha, Vitor de Cinque Almeida, C. Amado, J. E. Olivo, Oswaldo Curty da Motta Lima","doi":"10.1590/s2175-97902023e221000","DOIUrl":"https://doi.org/10.1590/s2175-97902023e221000","url":null,"abstract":"Cannabidiol (CBD) is a bioactive compound with promising anti-inflammatory results but has low aqueous solubility. Complexation of drugs with this characteristic in carriers is an alternative to improve their efficiency. This study aimed to prepare and characterize CBD complexes in different carriers, and to evaluate the anti-inflammatory effect of such preparations using an experimental model of edema induction in rat paws. The results were compared to a reference drug, ibuprofen (IBU). The carriers evaluated were beta cyclodextrin (bCD) and activated charcoal (AC). Quantification of the drugs in the complexes was determined, and different qualitative analyses were also performed. Oral treatments in single doses with CBD showed inhibitory effects similar to that of IBU, potentiating its bioactivity without significant adverse effects. CBD*bCD doses at 4.375, 8.75, 17.5, and 35 mg/kg significantly reduced the intensity of edema compared to equivalent doses of pure bioactive. In contrast, CBD*AC did not generate benefits. There was no significant inhibitory effect on myeloperoxidase activity, requiring more specific analyses to assess this parameter. The results suggest that the CBD*bCD complexation is perfectly feasible, increasing its anti-edematogenic efficacy in the experimental model used.","PeriodicalId":9218,"journal":{"name":"Brazilian Journal of Pharmaceutical Sciences","volume":null,"pages":null},"PeriodicalIF":1.3,"publicationDate":"2023-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67742203","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Advancements in artificial intelligence and machine learning in revolutionising biomarker discovery 人工智能和机器学习在彻底改变生物标志物发现方面的进展

IF 1.3 4区医学

Brazilian Journal of Pharmaceutical Sciences Pub Date : 2023-08-28 DOI: 10.1590/s2175-97902023e23146

G. S. Raikar, A. S. Raikar, S. Somnache

引用次数: 0

Investigation on formulation parameters of donepezil HCl loaded solid lipid nanoparticles 盐酸多奈哌齐固体脂质纳米粒子的处方参数研究

IF 1.3 4区医学

Brazilian Journal of Pharmaceutical Sciences Pub Date : 2023-08-28 DOI: 10.1590/s2175-97902023e22330

Gizem Rüya Topal, Berrin Küçüktürkmen, U. Öz, Erva Özkan, Filiz Bakar-Ates, A. Bozkır

{"title":"Investigation on formulation parameters of donepezil HCl loaded solid lipid nanoparticles","authors":"Gizem Rüya Topal, Berrin Küçüktürkmen, U. Öz, Erva Özkan, Filiz Bakar-Ates, A. Bozkır","doi":"10.1590/s2175-97902023e22330","DOIUrl":"https://doi.org/10.1590/s2175-97902023e22330","url":null,"abstract":"symptomatic treatment of Alzheimer’s disease (AD) and has many side effects. In this study, to reduce the side effects of Donepezil-HCl and increase the penetration of the drug through the blood-brain barrier, we aimed to design a solid lipid nanoparticle (SLN) formulation. The effects of the different formulation parameters, such as homogenization speed, sonication time, lipid and drug concentration, surfactant type and concentration, and volume of the aqueous phase, were assessed for optimization. The particle size and PDI increased with increasing lipid concentration but decreased with increasing amounts of surfactant (Tween 80) and co-surfactant (lecithin). When the homogenization rate and sonication time increased, the particle size decreased and the encapsulation efficiency increased. The optimized formulation exhibited particle size, PDI, encapsulation efficiency, and zeta potential of 87.2±0.11 nm; 0.22±0.02; 93.84±0.01 %; -17.0±0.12 mV respectively. The in vitro release investigation revealed that approximately 70% of Donepezil-HCl was cumulatively released after 24 hours. TEM analysis proved that spherical and smooth particles were obtained and formulations had no toxic effect on cells. The final optimized formulation could be a candidate for Donepezil-HCl application in Alzheimer’s treatment with reduced side effects and doses for patients.","PeriodicalId":9218,"journal":{"name":"Brazilian Journal of Pharmaceutical Sciences","volume":null,"pages":null},"PeriodicalIF":1.3,"publicationDate":"2023-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67742096","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Determination of cyanide in whole seeds and brans of linseed (Linum usitatissimum Linn) by molecular spectrophotometry 分子分光光度法测定亚麻籽(Linum usitatissimum Linn)中氰化物的含量

IF 1.3 4区医学

Brazilian Journal of Pharmaceutical Sciences Pub Date : 2023-08-28 DOI: 10.1590/s2175-97902023e23059

Marcelle Leandro da Silva Pereira, Rita de Cássia Paulino de Souza, Juliana Vilar Furtado de Medeiros, George Queiroz de Brito, A. Schwarz

{"title":"Determination of cyanide in whole seeds and brans of linseed (Linum usitatissimum Linn) by molecular spectrophotometry","authors":"Marcelle Leandro da Silva Pereira, Rita de Cássia Paulino de Souza, Juliana Vilar Furtado de Medeiros, George Queiroz de Brito, A. Schwarz","doi":"10.1590/s2175-97902023e23059","DOIUrl":"https://doi.org/10.1590/s2175-97902023e23059","url":null,"abstract":"The addition of linseed ( Linum usitatissimum Linn) in the diet, as a functional food, has increased over the years. However, it possesses cyanogenic glycosides. This study aimed to quantify and compare cyanide concentration in whole seed and bran of brown and golden types to establish a safe limit of intake. Three commercial labels, from brown and golden whole seed types (Ab, Ag, Bb, Bg, Cb and Cg), and six commercial labels of brown and golden bran (1b, 2g, 3g, 4b, 5g, and 6b), were selected, totalizing twelve samples. Total cyanide concentration was quantified by a colorimetric method employing alkaline picrate, after acid hydrolysis. The whole seed cyanide values were between 348.4 and 473.20 µg/g and the bran cyanide values were between 459.53 and 639.35 μg/g. The analyzed bran presented increased cyanide concentrations than the whole seeds with no differences between brown and golden types. Food able to produce cyanide less than 90 µg/kg body weight, daily, is considered secure for consumption. Considering this limit and analyzed samples, it is safe to eat approximately two tablespoons of seeds or one tablespoon of bran. These results point out the importance of cyanide amount daily intake information to be in linseed packaging, to ensure secure consumption.","PeriodicalId":9218,"journal":{"name":"Brazilian Journal of Pharmaceutical Sciences","volume":null,"pages":null},"PeriodicalIF":1.3,"publicationDate":"2023-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67743019","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Swertiamarin attenuates paraquat-induced pulmonary epithelial-like cell apoptosis via NOX4-mediated regulation of redox and mitochondrial function Swertiamarin通过NOX4介导的氧化还原和线粒体功能调节减轻百草枯诱导的肺上皮样细胞凋亡

IF 1.3 4区医学

Brazilian Journal of Pharmaceutical Sciences Pub Date : 2023-08-28 DOI: 10.1590/s2175-97902023e22476

Ji Wu, Yong Li, Xinju Li, Tao Wu

{"title":"Swertiamarin attenuates paraquat-induced pulmonary epithelial-like cell apoptosis via NOX4-mediated regulation of redox and mitochondrial function","authors":"Ji Wu, Yong Li, Xinju Li, Tao Wu","doi":"10.1590/s2175-97902023e22476","DOIUrl":"https://doi.org/10.1590/s2175-97902023e22476","url":null,"abstract":"The aim of the present study was to investigate the effect of swertiamarin (STM) in attenuating paraquat (PQ)-induced human lung alveolar epithelial-like cell (A549) apoptosis and the underlying mechanisms. A549 cells were pretreated with different concentrations of STM for 2 hr and then cultured with or without PQ (700 μM) for 24 hr. Cell survival was determined using the CCK8 assay. Morphological changes, MDA content, inflammatory factors, fibrogenesis parameters, apoptosis rates, redox status and mitochondrial membrane potential (MMP) were evaluated. The expression of several genes involved in the modulation of redox status was measured by Western blotting. Cell viability and MMP were decreased, but the apoptosis rate and DCFH oxidation were elevated by PQ exposure. STM pretreatment notably increased cell viability and MMP and reduced the apoptosis rate and DCFH oxidation. Furthermore, TLR4-NOX4 signaling was significantly inhibited by STM. The downregulation of NOX4 by siRNA exerted the same protective effects as STM. This study provides the first evidence that STM attenuates PQ-induced pulmonary epithelial-like cell apoptosis via NOX4-mediated regulation of redox and mitochondrial function.","PeriodicalId":9218,"journal":{"name":"Brazilian Journal of Pharmaceutical Sciences","volume":null,"pages":null},"PeriodicalIF":1.3,"publicationDate":"2023-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67741942","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Research on Anti-inflammatory Targets and Mechanisms of alkaloids in Picrasma quassioides Benn Through Network Pharmacology 网络药理学研究拟甾体坏死病生物碱抗炎作用靶点及机制

IF 1.3 4区医学

Brazilian Journal of Pharmaceutical Sciences Pub Date : 2023-08-28 DOI: 10.1590/s2175-97902023e22394

Qijia Xu, Kai Wang, Yaoyao Xu, Yinhe Gao, Ge Wang, Sheng Liu, Feng Zhao

引用次数: 0

Effect of polycaprolactone nanocapsules loaded with essential oils on biofilm formation by Staphylococcus aureus strains isolated from bovine mastitis cases 精油负载聚己内酯纳米胶囊对牛乳腺炎金黄色葡萄球菌生物膜形成的影响

IF 1.3 4区医学

Brazilian Journal of Pharmaceutical Sciences Pub Date : 2023-08-28 DOI: 10.1590/s2175-97902023e23068

Gabriela Suthovski, Alcione Santa Catarina, Diana Paula Perin, R. Mainardes, Karina Ramirez Starikoff, A. Gallina, Maiara Garcia Blagitz Azevedo, F. Dalmolin, Luciana Velasques Cervo, D. M. Benvegnú

{"title":"Effect of polycaprolactone nanocapsules loaded with essential oils on biofilm formation by Staphylococcus aureus strains isolated from bovine mastitis cases","authors":"Gabriela Suthovski, Alcione Santa Catarina, Diana Paula Perin, R. Mainardes, Karina Ramirez Starikoff, A. Gallina, Maiara Garcia Blagitz Azevedo, F. Dalmolin, Luciana Velasques Cervo, D. M. Benvegnú","doi":"10.1590/s2175-97902023e23068","DOIUrl":"https://doi.org/10.1590/s2175-97902023e23068","url":null,"abstract":"Bovine infectious mastitis is largely resistant to antibacterial treatment, mainly due to mechanisms of bacterial resistance in the biofilms formed by Staphylococcus aureus. Melaleuca (MEO) and citronella essential oils (CEO) are promising agents for reducing or eliminating biofilms. Free melaleuca oil presented a medium Minimum Inhibitory Concentration (MIC) of 0.625% and a Minimum Bactericidal Concentration (MBC) of 1.250%, while free citronella oil showed medium MIC and MBC of 0.313%. Thus, free CEO and MEO demonstrate bacteriostatic and bactericidal potential. We generated polymeric nanocapsules containing MEO or CEO and evaluated their efficacy at reducing biofilms formed by S. aureus . Glass and polypropylene spheres were used as test surfaces. To compare the responses of free and encapsulated oils, strains were submitted to 10 different procedures, using free and nanoencapsulated essential oils (EOs) in vitro . We observed no biofilm reduction by MEO, free or nanoencapsulated. However, CEO nanocapsules reduced biofilm formation on glass (p = 0.03) and showed a tendency to diminish biofilms on polypropylene (p = 0.051). Despite nanoencapsulated CEO reducing biofilms in vitro , the formulation could be improved to modify the CEO component polarity and, including MEO, to obtain more interactions with surfaces and the biofilm matrix.","PeriodicalId":9218,"journal":{"name":"Brazilian Journal of Pharmaceutical Sciences","volume":null,"pages":null},"PeriodicalIF":1.3,"publicationDate":"2023-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67743188","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0