Effect of polycaprolactone nanocapsules loaded with essential oils on biofilm formation by Staphylococcus aureus strains isolated from bovine mastitis cases

IF 0.6 4区医学 Q4 PHARMACOLOGY & PHARMACY

Brazilian Journal of Pharmaceutical Sciences Pub Date : 2023-08-28 DOI:10.1590/s2175-97902023e23068

Gabriela Suthovski, Alcione Santa Catarina, Diana Paula Perin, R. Mainardes, Karina Ramirez Starikoff, A. Gallina, Maiara Garcia Blagitz Azevedo, F. Dalmolin, Luciana Velasques Cervo, D. M. Benvegnú

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引用次数: 0

Abstract

Bovine infectious mastitis is largely resistant to antibacterial treatment, mainly due to mechanisms of bacterial resistance in the biofilms formed by Staphylococcus aureus. Melaleuca (MEO) and citronella essential oils (CEO) are promising agents for reducing or eliminating biofilms. Free melaleuca oil presented a medium Minimum Inhibitory Concentration (MIC) of 0.625% and a Minimum Bactericidal Concentration (MBC) of 1.250%, while free citronella oil showed medium MIC and MBC of 0.313%. Thus, free CEO and MEO demonstrate bacteriostatic and bactericidal potential. We generated polymeric nanocapsules containing MEO or CEO and evaluated their efficacy at reducing biofilms formed by S. aureus . Glass and polypropylene spheres were used as test surfaces. To compare the responses of free and encapsulated oils, strains were submitted to 10 different procedures, using free and nanoencapsulated essential oils (EOs) in vitro . We observed no biofilm reduction by MEO, free or nanoencapsulated. However, CEO nanocapsules reduced biofilm formation on glass (p = 0.03) and showed a tendency to diminish biofilms on polypropylene (p = 0.051). Despite nanoencapsulated CEO reducing biofilms in vitro , the formulation could be improved to modify the CEO component polarity and, including MEO, to obtain more interactions with surfaces and the biofilm matrix.

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来源期刊

Brazilian Journal of Pharmaceutical Sciences PHARMACOLOGY & PHARMACY-

CiteScore

1.40

自引率

0.00%

发文量

165

审稿时长

7.5 months

期刊介绍： The Brazilian Journal of Pharmaceutical Sciences accepts for publication Original Papers applicable to the fields of Pharmaceutical Sciences; Reviews and Current Comment Articles, which are published under the Scientific Editor and Associate Editors invitation to recognized experts or when they are spontaneously submitted by the authors in the form of abstracts to have their importance evaluated. A critical view of the subject with insertions of results of previous works in the field in relation to the state of art must be included; Short Communications reporting new methods and previews of works on researches of outstanding importance in which originality justify a quick publication. A maximum of 2000 words excluding tables, figures and references is an acceptable limit. One table, one figure and ten references may be added, and Book Reviews of the latest editions of books, prepared by specialists invited by the Scientific Editor and Associate Editors. Thematic Supplements as well as those related to scientific meetings can be published under the Scientific Editor and/or Associate Editors agreement.