Brazilian dental journal最新文献_第7页

Fracture resistance of pressed ZLS crowns versus pressed LD crowns under thermo-mechanical cycling. 加压ZLS冠与加压LD冠在热机械循环下的抗断裂性能。

Brazilian dental journal Pub Date : 2022-11-01 DOI: 10.1590/0103-6440202204993

Basma Osama Salem, Dina Magdy Elshehawi, Gihan Abdelhady Elnaggar

{"title":"Fracture resistance of pressed ZLS crowns versus pressed LD crowns under thermo-mechanical cycling.","authors":"Basma Osama Salem, Dina Magdy Elshehawi, Gihan Abdelhady Elnaggar","doi":"10.1590/0103-6440202204993","DOIUrl":"https://doi.org/10.1590/0103-6440202204993","url":null,"abstract":"The aim of this study was conducted to assess the fracture resistance of zirconia reinforced lithium silicate all ceramic material \"Celtra Press\" compared to lithium disilicate one \"IPS e-max Press\" under simulated oral conditions. Fourteen ceramic crowns were fabricated on epoxy dies which were duplicated from stainless steel master die and divided into two equal groups (n=7) according to the material of construction; Group A: Crowns fabricated with IPS e-max Press material and Group B: Crowns fabricated with Celtra Press material. The crowns were then cemented onto their corresponding dies with a self-adhesive resin cement and subjected to thermocycling and cyclic loading. Then they were loaded to fractur in a universal testing machine. The results were tabulated and statistically analyzed using Kolmogorov-Smirnov and Shapiro-Wilk tests. Student t-test used to compare mean values. The significance level was set at P ≤ 0.05 and 95% Confidence interval. Statistical analysis was performed using Graph Pad Instat (Graph Pad, Inc.) software for windows. The mean ± SD values of fracture resistance were recorded for lithium Disilicate group (1706.01 ±154.32 N) meanwhile the mean ± SD value recorded with celtra group were (1550.67±196.71 N). Zirconia reinforced lithium silicate ceramic crowns produced comparable fracture resistance values to lithium disilicate ceramic crowns and both tested materials are within the clinically acceptable values in the posterior area.","PeriodicalId":9211,"journal":{"name":"Brazilian dental journal","volume":"33 6","pages":"103-109"},"PeriodicalIF":0.0,"publicationDate":"2022-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9733374/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10328518","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development and professional validation of an App to support Oral Cancer Screening. 开发和专业验证支持口腔癌筛查的应用程序。

Brazilian dental journal Pub Date : 2022-11-01 DOI: 10.1590/0103-6440202204895

Talita Jordânia Rocha do Rêgo, José Vitor Mota Lemos, Amanda Pinheiro Leitão Matos, Caio Ferreira Freire Caetano, Thinali Sousa Dantas, Fabrício Bitu Sousa, Edgar Marçal de Barros Filho, Paulo Goberlânio de Barros Silva

{"title":"Development and professional validation of an App to support Oral Cancer Screening.","authors":"Talita Jordânia Rocha do Rêgo, José Vitor Mota Lemos, Amanda Pinheiro Leitão Matos, Caio Ferreira Freire Caetano, Thinali Sousa Dantas, Fabrício Bitu Sousa, Edgar Marçal de Barros Filho, Paulo Goberlânio de Barros Silva","doi":"10.1590/0103-6440202204895","DOIUrl":"https://doi.org/10.1590/0103-6440202204895","url":null,"abstract":"The objective of this study was to develop and validate an App for identifying risk factors for oral cancer. To this end, we developed an App (OCS: Oral Cancer Screening) with predictors of Oral Cancer (OC) and algorithm assembly to estimate the risk of its development.Methodology: Simulated clinical cases were designed so that 40 professionals with expertise in oral diagnostics could validate the algorithm and test its usability (SUS: System Usability Score) and acceptability (TAM: Technology Acceptance Model). Cronbach's alpha coefficient, Friedman/Dunn tests, and Spearman correlation evaluated the SUS and TAM scales. ROC curve was plotted to estimate the cutoff point of the algorithm in suggesting a high risk for OCS of the simulated cases. Chi-square and Fisher's exact tests were additionally used (p<0.05, SPSS v20.0).Results: Professionals with expertise in oral diagnosis had usability of 84.63±10.66 and acceptability of 84.75±10.62, which correlated positively (p<0.001, r=0.647). Acting in clinical areas of dentistry (p=0.034) and history of performing OC risk factor orientation (p=0.048) increased acceptability while acting in higher education increased usability (p=0.011). The cutoff point suggested by the App after validation of the simulated clinical cases showed high sensitivity of 84.8% and lower specificity of 58.4%.Conclusion: The OCS was effective and with adequate sensitivity, usability, and acceptability and may contribute to the detection of early oral lesions.","PeriodicalId":9211,"journal":{"name":"Brazilian dental journal","volume":"33 6","pages":"44-55"},"PeriodicalIF":0.0,"publicationDate":"2022-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9733366/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10406126","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Detoxification of LTA by intracanal medication: analysis by macrophages proinflammatory cytokines production. 龋内药物对 LTA 的解毒作用：通过巨噬细胞促炎细胞因子的产生进行分析。

Brazilian dental journal Pub Date : 2022-11-01 DOI: 10.1590/0103-6440202205195

Luciane Dias de Oliveira, Felipe Eduardo de Oliveira, Bárbara Araujo Hatje, Marcia Carneiro Valera, Cláudio Antonio Talge Carvalho, Amjad Abu Hasna

{"title":"Detoxification of LTA by intracanal medication: analysis by macrophages proinflammatory cytokines production.","authors":"Luciane Dias de Oliveira, Felipe Eduardo de Oliveira, Bárbara Araujo Hatje, Marcia Carneiro Valera, Cláudio Antonio Talge Carvalho, Amjad Abu Hasna","doi":"10.1590/0103-6440202205195","DOIUrl":"10.1590/0103-6440202205195","url":null,"abstract":"The aim of this study was to evaluate in vitro the effect of calcium hydroxide [Ca(OH)2], 2% chlorhexidine gel (CHX) on macrophages (RAW 264.7) to produce pro-inflammatory cytokines and nitric oxide after pretreatment with lipoteichoic acid (LTA) of Enterococcus faecalis. Forty-eight human single-rooted teeth were instrumented with R25.08 (RECIPROC) and sterilized by gamma irradiation. LTA was inoculated in the root canal of each specimen for 96 hours. Specimens were instrumented with 40.06 and 50.05 (RECIPROC) and medicated with: I) Pyrogen-free saline solution (SS); II) 2% CHX gel; III) Ca(OH)2 + SS; or IV) Ca(OH)2 + CHX for 14 days. Three samples (S) were performed of the root canal of each specimen at: S1) immediately after instrumentation; S2) after Ethylenediaminetetraacetic acid (EDTA); S3) after intracanal medication removal. Subsequent quantification of cytokines (IL-1β, TNF-α, MIP-1α, IP-10, G-CSF and IL-6) by immunosorbent assay (ELISA) and nitric oxide by the Griess method was carried-out. Data were submitted to a normality test and then analyzed with one-way ANOVA and Tukey test with a significance level of 5% using GraphPad Prism 6. Ca(OH)2 + SS and Ca(OH)2 + CHX presented lower levels of TNF-α, TNF-α, IL-6, G-CSF and nitric oxide. Ca(OH)2 + SS was the most effective in reducing MIP-1α. CHX was effective in reducing IL-6 and G-CSF. Therefore, the combined intracanal medication of calcium hydroxide and chlorhexidine is effective in reducing the cytokines TNF-α, IL-1β, IL-6, G-CSF and nitric oxide.","PeriodicalId":9211,"journal":{"name":"Brazilian dental journal","volume":"33 6","pages":"36-43"},"PeriodicalIF":0.0,"publicationDate":"2022-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9733371/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10352771","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Reciprocating Kinematics of X-Smart Plus, VDW Silver and, iRoot Endodontic Motors: A Comparison Between Real and Set Values. X-Smart Plus, VDW Silver和iRoot牙髓马达的往复运动学:实测值和设定值的比较。

Brazilian dental journal Pub Date : 2022-11-01 DOI: 10.1590/0103-6440202204855

Dieimes Braambati, Renata de Castro Monteiro Netto, Marcelo Santos Coelho, Adriana de Jesus Soares, Marcos Frozoni

{"title":"Reciprocating Kinematics of X-Smart Plus, VDW Silver and, iRoot Endodontic Motors: A Comparison Between Real and Set Values.","authors":"Dieimes Braambati, Renata de Castro Monteiro Netto, Marcelo Santos Coelho, Adriana de Jesus Soares, Marcos Frozoni","doi":"10.1590/0103-6440202204855","DOIUrl":"https://doi.org/10.1590/0103-6440202204855","url":null,"abstract":"This study assessed 3 endodontic motors, X-Smart Plus (Dentsply Sirona, Ballaigues, Switzerland), VDW.Silver Reciproc (VDW GmbH, München, Germany) and, iRoot (Bassi Endodontics, Belo Horizonte, Brazil) in 2 different reciprocating settings. The movements evaluated were 170° in counter-clockwise (CCW) and 50° in clockwise (CW) at 350 RPM, and 150° CCW and 30° CW at 300 RPM. For the X-Smart Plus and VDW Silver the settings used were the ones in the motor library. For the iRoot, the motor was adjusted to the angles of the study. A customized optic target was attached to the contra-angle of the motor and the movements were recorded with a high-resolution camera (K2 DistaMaxTM Long-Distance Microscope System, Infinity Photo-Optical Company, Colorado, EUA) at 2,400 frames per second (FPS). The images were analyzed with the Vision Research software (Inc. Headquarters, Wayne, New Jersey, EUA). The following kinematic parameters were assessed: CCW angle, CW angle, speed (RPM) at both directions, and, standstill time at each change of directions. The Intraclass Correlation Coefficient (ICC) and Kruskal-Wallis (method of Dunn) were used at a significant level of 5%. There was no statistically significant difference among the motors at the 150°/30° setting (P > .05); the iRoot was the least reliable at the 170°/50° setting for CCW angle, speed, and net angle parameters (P < 0.05). The standstill time of all motors in both directions was identical. None of the motors were able to reproduce faithfully the set movements. The iRoot motor presented a higher discrepancy when compared to X-Smart and VDW Silver.","PeriodicalId":9211,"journal":{"name":"Brazilian dental journal","volume":"33 6","pages":"28-35"},"PeriodicalIF":0.0,"publicationDate":"2022-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9733375/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10406125","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Implants with hydrophilic surfaces equalize the osseointegration of implants in normo- and hyperglycaemic rats. 具有亲水性表面的种植体可使正常和高血糖大鼠的种植体骨整合平衡。

Brazilian dental journal Pub Date : 2022-11-01 DOI: 10.1590/0103-6440202204793

Felipe Eduardo Pinotti, Mauricio Andrés Tinajero Aron, Guilherme José Pimentel Lopes de Oliveira, Elcio Marcantonio Junior, Rosemary Adriana Chiérici Marcantonio

{"title":"Implants with hydrophilic surfaces equalize the osseointegration of implants in normo- and hyperglycaemic rats.","authors":"Felipe Eduardo Pinotti, Mauricio Andrés Tinajero Aron, Guilherme José Pimentel Lopes de Oliveira, Elcio Marcantonio Junior, Rosemary Adriana Chiérici Marcantonio","doi":"10.1590/0103-6440202204793","DOIUrl":"https://doi.org/10.1590/0103-6440202204793","url":null,"abstract":"The purpose of this study was to evaluate the effect of a surface modified by blasting and acid attack and maintained in an isotonic solution compared to a machined surface on osseointegration in normo- and hyperglycaemic animals. Sixty-four animals were allocated into 4 groups with 16 animals each, and they were subdivided into two experimental periods (15 and 45 days), with 8 animals in each group. The groups were divided according to the type of implant that was installed in the animals' tibia and the animals' systemic condition: CM - Machined implants placed in Healthy animals; CH - Hydrophilic implants placed in Healthy animals, HM - Machined implants placed in animals with hyperglycaemia; HH- Hydrophilic implants installed in animals with hyperglycaemia. The following analyses were performed: biomechanical (removal torque), microtomographic (evaluation of the bone volume around the implants- BV/TV), and histomorphometric (evaluation of bone-implant contact BIC% and of the bone formation area between the threads BBT%). It was found that the implants with hydrophilic surfaces presented higher removal torques and quantities of BV/TV% and higher BIC% and BBT% values in normo- and hyperglycaemic animals. The results of this study indicated that the hydrophilic surface accelerates the osseointegration process (~ 15% BIC/BBT at 15-day period), especially in animals with hyperglycaemia. The hydrophilic surface equaled the osseointegration between normo- and hyperglycaemic animals, reversing the negative potential of hyperglycaemia on the osseointegration process.","PeriodicalId":9211,"journal":{"name":"Brazilian dental journal","volume":"33 6","pages":"71-77"},"PeriodicalIF":0.0,"publicationDate":"2022-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9733365/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10345051","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Three-dimensional assessment of internal adaptation measurement of three cad/cam ceramic systems. 三种cad/cam陶瓷系统内适应性测量的三维评价。

Brazilian dental journal Pub Date : 2022-11-01 DOI: 10.1590/0103-6440202204981

Emad M Elsharkawy, Ahmed My ElKouedi, Tamer E Shokry

{"title":"Three-dimensional assessment of internal adaptation measurement of three cad/cam ceramic systems.","authors":"Emad M Elsharkawy, Ahmed My ElKouedi, Tamer E Shokry","doi":"10.1590/0103-6440202204981","DOIUrl":"https://doi.org/10.1590/0103-6440202204981","url":null,"abstract":"This study aimed to evaluate the internal adaptation of three different computer-aided design/computer-aided manufacturing (CAD/CAM) ceramic crowns. The internal adaptation of a polymer-infiltrated ceramic network material (Vita Enamic [VE]) was compared to two machinable glass-ceramics; Zirconia-reinforced lithium silicate (Vita Suprinity [VS]) and a lithium disilicate glass-ceramic (IPS e.max. CAD). Thirty human premolars of average size were prepared (n=10 each group) by computer numerical control to fulfill the criteria of all-ceramic crown design. Optical impressions were taken for each tooth preparation using the CAD/CAM scanner. Thirty crowns were fabricated using CAD/CAM system and divided into three groups (IPS e.max, VE, and VS). To assess the internal fit of tested crowns, the gap between the intaglio of each crown and the corresponding tooth surface was evaluated using a 3D digital scanner using the Triple-scan Protocol. One-way ANOVA followed by Tukey Post Hoc statistical tests were used to statistically analyze results of the internal fit. There was a statistically significant difference for all groups at the four axial walls (p = 0.000002). For total internal fit between groups, comparisons showed a statistically significant difference between all tested groups (p=0.000002). When each pair of groups was statistically compared with each other, all pair comparisons showed a statistically significant difference. IPS e.max CAD had the best internal fit, followed by Vita Enamic, then Vita Suprinity. For all ceramics tested, values of internal fit of all ceramics tested were within the clinically acceptable range.","PeriodicalId":9211,"journal":{"name":"Brazilian dental journal","volume":"33 6","pages":"121-128"},"PeriodicalIF":0.0,"publicationDate":"2022-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9733377/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10406123","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of dental tissue thickness on the measurement of oxygen saturation by two different pulse oximeters 牙组织厚度对两种不同脉搏血氧计测量血氧饱和度的影响

Brazilian dental journal Pub Date : 2022-10-01 DOI: 10.1590/0103-6440202204903

D. H. N. Henriques, A. M. H. Alves, M. Kuntze, L. F. R. Garcia, E. A. Bortoluzzi, C. Teixeira

{"title":"Effect of dental tissue thickness on the measurement of oxygen saturation by two different pulse oximeters","authors":"D. H. N. Henriques, A. M. H. Alves, M. Kuntze, L. F. R. Garcia, E. A. Bortoluzzi, C. Teixeira","doi":"10.1590/0103-6440202204903","DOIUrl":"https://doi.org/10.1590/0103-6440202204903","url":null,"abstract":"Abstract This study aimed to evaluate the influence of different dental tissue thickness on the measurement of oxygen saturation (SpO2) levels in high (HP) and low (LP) blood perfusion by comparing the values obtained from two different pulse oximeters (POs) - BCI and Sense 10. Thirty freshly extracted human teeth had their crowns interposed between the POs and an optical simulator, which emulated the SpO2 and heart beats per minute (bpm) at HP (100% SpO2/75 bpm) and LP (86% SpO2/75 bpm) modes. Afterwards, the palatine/lingual surfaces of the dental crowns were worn with diamond drills. The reading of SpO2 was performed again using the POs alternately through the buccal surface of each dental crown. Data were analyzed by the Wilcoxon, Mann-Whitney and Kendall Tau-b tests (α=5%). The results showed significant difference at the HP and LP modes in the SpO2 readouts through the different dental thicknesses with the use of BCI, and at the LP mode with the use of Sense 10, which had a significant linear correlation (p<0.0001) and lower SpO2 readout values in relation to the increase of the dental thickness. Irrespective of tooth thickness, Sense 10 had significantly higher readout values (p<0.0001) than BCI at both perfusion modes. The interposition of different thicknesses of enamel and dentin influenced the POs measurement of SpO2, specially at the low perfusion mode. The POs were more accurate in SpO2 measurement when simulated perfusion levels were higher.","PeriodicalId":9211,"journal":{"name":"Brazilian dental journal","volume":"33 1","pages":"26 - 34"},"PeriodicalIF":0.0,"publicationDate":"2022-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45773638","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Analysis of the mandibular molars root canals morphology. Study by computed tomography 下颌磨牙根管形态分析。计算机断层扫描研究

Brazilian dental journal Pub Date : 2022-10-01 DOI: 10.1590/0103-6440202205105

Verônica de Oliveira Mantovani, Aline Evangelista de Souza Gabriel, R. Silva, R. Savioli, M. Sousa-Neto, A. Cruz-Filho

{"title":"Analysis of the mandibular molars root canals morphology. Study by computed tomography","authors":"Verônica de Oliveira Mantovani, Aline Evangelista de Souza Gabriel, R. Silva, R. Savioli, M. Sousa-Neto, A. Cruz-Filho","doi":"10.1590/0103-6440202205105","DOIUrl":"https://doi.org/10.1590/0103-6440202205105","url":null,"abstract":"Abstract This study aimed to analyze the root and root canal morphology of mandibular first and second molars using CBCT images. A total of 2,400 mandibular molars exams were selected from 600 patients aged between 18 and 75 years. The number of roots, number of root canals, and root canal configuration according to the Vertucci classification were verified. Overall, 94.92% of mandibular first molars and 90.17% of mandibular second molars had two separate roots. Among the biradicular molars, the first molars showed a greater incidence of type IV canals in the mesial root and type I in the distal root. In the second molars, the most common canal form was type IV in the mesial root and type I in the distal root. In the triradicular molars, the type IV and type I configurations were the most common in the mesial root of the first molar and second molar, respectively. In both triradicular molars, there was a prevalence of type I canal in the distal and DL roots. Statistical analysis was performed at a significance level of 0.05. The number of roots was correlated with gender (Spearman test), and the canal's configuration with gender and bilaterality (Wilcoxon test). The subpopulation studied has a high incidence of bilateral symmetry and mandibular molars with two roots with two distinct mesial canals and one distal canal. The bilateral configuration is possible to estimate the number of canals, especially in images that are difficult to visualize, such as atresic canals.","PeriodicalId":9211,"journal":{"name":"Brazilian dental journal","volume":"33 1","pages":"1 - 8"},"PeriodicalIF":0.0,"publicationDate":"2022-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44475159","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Effect of phytic acid on bond strength and interfacial integrity of universal adhesive to deep dentin 植酸对深层牙本质万能胶结合强度和界面完整性的影响

Brazilian dental journal Pub Date : 2022-10-01 DOI: 10.1590/0103-6440202204810

A. Attia, A. Abo-Elezz, R. Safy

{"title":"Effect of phytic acid on bond strength and interfacial integrity of universal adhesive to deep dentin","authors":"A. Attia, A. Abo-Elezz, R. Safy","doi":"10.1590/0103-6440202204810","DOIUrl":"https://doi.org/10.1590/0103-6440202204810","url":null,"abstract":"Abstract This study investigated the effect of phytic acid (IP6) in different concentrations and application times on microtensile bond strength (µTBS) and interfacial integrity of universal adhesive to deep dentin. Flat deep dentin surfaces of 48 human molars were either etched with 37% phosphoric acid (PA) for 15 sec (control); or received no acid treatment and universal adhesive was applied directly in a self-etch mode (SE); or divided according to IP6 concentration (C) into two main groups: C1, 0.5%, and C2, 1%. Specimens of IP6 groups were further subdivided into three subgroups according to application time of IP6 (T) where; T1, 15 sec; T2, 30 sec and T3, 60 sec. Single Bond Universal Adhesive was then applied and resin composite blocks were built-up. Forty Specimens were then sectioned to produce resin/dentin beams that were used for µTBS testing using a universal testing machine. The remaining eight specimens were sectioned into slabs that were immersed into ammonical silver nitrate solution and nanoleakage was observed using a field emission scanning electron microscope (FE-SEM). The results showed that the application of IP6 in 0.5% and 1% produced significantly higher µTBS and less nanoleakage compared to PA and SE groups. Also, the application of IP6 for 60 sec recorded the highest µTBS and the lowest nanoleakage followed by 30 sec, and 15 sec respectively. Therefore, conditioning of deep dentin with IP6 enhances µTBS and interfacial integrity of universal adhesive to deep dentin in comparison to PA etching or using the universal adhesive in SE mode.","PeriodicalId":9211,"journal":{"name":"Brazilian dental journal","volume":"33 1","pages":"116 - 125"},"PeriodicalIF":0.0,"publicationDate":"2022-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42054117","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Leukotriene B4 Loaded in Microspheres Inhibits Osteoclast Differentiation and Activation 微球负载白三烯B4抑制破骨细胞分化和活化

Brazilian dental journal Pub Date : 2022-10-01 DOI: 10.1590/0103-6440202204827

Francine Lorencetti-Silva, M. M. Arnez, João Pedro de Queiroz Thomé, M. Carvalho, F. Carvalho, A. M. Queiroz, L. Faccioli, F. W. G. Paula-Silva

{"title":"Leukotriene B4 Loaded in Microspheres Inhibits Osteoclast Differentiation and Activation","authors":"Francine Lorencetti-Silva, M. M. Arnez, João Pedro de Queiroz Thomé, M. Carvalho, F. Carvalho, A. M. Queiroz, L. Faccioli, F. W. G. Paula-Silva","doi":"10.1590/0103-6440202204827","DOIUrl":"https://doi.org/10.1590/0103-6440202204827","url":null,"abstract":"Abstract To investigate osteoclast formation in vivo and if leukotriene B4 (LTB4) loaded in microspheres (MS) could be used as a therapeutical strategy to promote a sustained delivery of the mediator and prevent osteoclast differentiation. Methods: In vivo, apical periodontitis was induced in mice to investigate osteoclast differentiation and signaling in absence of 5-lipoxygenase (5-LO). In vitro, LTB4-MS were prepared using an oil-in-water emulsion solvent extraction-evaporation process. Characterization and efficiency of LTB4 encapsulation were investigated. J774A.1 macrophages were cultured in the presence of monocyte colony-stimulating factor (M-CSF) and ligand for receptor activator of nuclear factor kappa B (RANKL) and then stimulated with LTB4-MS. Cytotoxicity, in vitro MS-LTB4 uptake, osteoclast formation and gene expression were measured. Results: We found that 5-LO negatively regulates osteoclastic formation in vivo during apical periodontitis development. In vitro, LTB4-MS were up-taken by macrophages and were not cytotoxic to the cells. LTB4-MS inhibited osteoclast formation and the synthesis of osteoclastogenic genes Acp5, Mmp9, Calcr and Ctsk. LTB4-MS inhibited differentiation of macrophages into an osteoclastic phenotype and cell activation under M-CSF and RANKL stimulus.","PeriodicalId":9211,"journal":{"name":"Brazilian dental journal","volume":"33 1","pages":"35 - 45"},"PeriodicalIF":0.0,"publicationDate":"2022-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47472563","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2