Journal of molecular biology research最新文献

{"title":"Molecular Evaluation of HIV-1 HAART Efficacy, Comparison of TDF+3TC+EFV and AZT+3TC+NVP Regimens","authors":"Babacar Faye, Mame Diarra Bousso Lam, Ismaïl Barkiré, Micailou Magassouba, Cathy Cisse, Aissatou Ngom, Alioune Dièye","doi":"10.5539/jmbr.v12n1p10","DOIUrl":"https://doi.org/10.5539/jmbr.v12n1p10","url":null,"abstract":"Context: The UNAIDS goal of ending the HIV epidemic by 2030 will be achieved primarily through the success of the three “95s” by 2030, and in particular the third “95 », which consists of achieving an undetectable viral load thanks to effective antiretroviral treatment. Achieving and maintaining viral suppression is the goal of ART and a good knowledge of the effectiveness of triple ARV therapies can help national HIV programs. Thus, the objective of this study was to evaluate and compare the effectiveness of the two ARTs, Tenovofir + Lamivudine +Efavirenz (TDF+3TC+EFV) and Zidovudine+Lamivudine+ Efavirenz (AZT+3TC+NVP) in their abilities to make the plasma viral load of HIV+ patients under treatment undetectable. Material and method: This is a retrospective study of the management of HIV-1 seropositive patients, followed at the Military Hospital of Ouakam (HMO) Molecular Biology laboratory from 2014 to 2021. The main criterion for evaluating the effectiveness of the treatment was the proportions of patients whose Viral load values were undetectable, VL< 50 copies/ml depending on duration of antiretroviral therapy (ART). Plasma viral load tests were carried out on Abbott Real Time HIV-1® (m2000sp/rt) and COBAS®AmpliPrep/COBAS®TaqMan® (Roche) version 2.0. Variables with p<0.05 were considered statistically significant for all comparisons between groups. Results: 3,335 patients met the inclusion criteria, including 2,078 on TDF+3TC+EFV, 445 on AZT+3TC+NVP and 812 were excluded due to death, transfer or loss to follow-up. At 6 months of ART, VL was undetectable in 7.3% of patients on TDF+3TC+EFV and 6.7% of patients on AZT+3TC+NVP (P=0.67). 78.2% versus 73.4% of patients had an undetectable VL respectively for TDF+3TC+EFV and AZT+3TC+NVP at 12 months of ART (p=0.03). TDF+3TC+EFV had significantly higher virological success rates than AZT+3TC+NVP (85.7% versus 80.2%) and virological failure (VL>1000 copies/ml) was significantly greater in patients taking AZT+3TC+NVP (13% versus 7.2%) after 18 months (P=0.001). Gender and age had a significant relationship in treatment success. Conclusion: TDF+3TC+EFV was superior in terms of virological suppression at the end of the study period. These results support the WHO recommendation to use TDF+3TD+EFV as an alternative first-line regimen.","PeriodicalId":92078,"journal":{"name":"Journal of molecular biology research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-11-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139252960","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cytosol-Exposed Lysine Residues Affect the Cytosol-Membrane Distribution and Serine-129 Phosphorylation of Alpha-Synuclein","authors":"Shi-you Lu","doi":"10.5539/jmbr.v12n1p1","DOIUrl":"https://doi.org/10.5539/jmbr.v12n1p1","url":null,"abstract":"Familial Parkinson’s disease and other neurodegenerative diseases known as synucleinopathies are strongly associated with α-Synuclein (αS) missense mutations. One of these mutations, E46K, had been hypothesized to increase the electrostatic attraction of αS to neuronal vesicle membranes due to positively charged lysine that attracts negatively charged phospholipid head groups. Here, we confirm the biochemical mechanism of E46K through four compound mutants, each with three replacements with lysine on a cytosol-exposed position of the αS alpha helix. We show that the cytosolic αS to membrane-bound αS ratios are significantly lower, and that the phosphorylation rates of serine-129-a pathological marker-are pronouncedly higher for the mutants than for wild type. This experiment addresses the previous knowledge gap in the understanding of basic amino acid replacements in cytosol-exposed positions of αS. Importantly, the validated effect of cytosol-exposed lysine residues has implications for exploring the mechanism of pathogenesis of αS mutants in familial Parkinson’s disease.","PeriodicalId":92078,"journal":{"name":"Journal of molecular biology research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-01-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43086872","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phorbol 12-myristate 13-acetate (PMA) Alters Galectin Gene Expression in Cows","authors":"B. Mulakala, M. Worku","doi":"10.5539/jmbr.v11n1p1","DOIUrl":"https://doi.org/10.5539/jmbr.v11n1p1","url":null,"abstract":"Galectins (GAL) are β-galactoside binding proteins. The objective of this study was to assess the effect of PMA on the regulation of galectin gene expression in bovine blood and neutrophils. Blood was collected from 3 clinically healthy Holstein cows. Neutrophils were isolated by differential centrifugation. Blood and neutrophils were treated with 10ng/ml of PMA or maintained in phosphate-buffered saline for 30 minutes at 370 C. Pooled total RNA were transcribed to cDNA for real-time PCR. Specific primers for bovine LGALS -1, -2, -3, -4, -7, -8, -9, -11, -12, and their binding proteins (bp) LGALS3bp, T cell immunoglobulin and mucin domain 3 (TIM-3) were used. Housekeeping genes RPLP0 and UCHL5 served as internal controls. Fold changes were calculated using the Livak method. Total protein concentration was assessed using the Bicinchoninic acid assay. Secretion of GAL -1, -2, -3, -4, -8, -9 was assessed using bovine GAL specific ELISA. All tested LGALS were detected. Activation with PMA differentially modulated expression and secretion of GAL in blood and neutrophils. Expression levels of LGALS -1 and LGALS -3 was not affected. Expression of LGALS -7, -8, -9, -11, -12, and TIM-3 was differentially modulated. The secretion of GAL -3 was significantly increased in response to PMA. The most pronounced effect of PMA was observed on LGALS-9, TIM-3 expression, and the secretion of GAL -3. Thus, GAL may serve as biomarkers of cell activation with functional consequences and warrant further study.","PeriodicalId":92078,"journal":{"name":"Journal of molecular biology research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-10-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47267404","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Emergency Nurses Job Satisfaction Prediction Model: Personality traits, Resilience, Emotional Expression and Ambiguity Tolerance","authors":"Sahar Eghbali, M. Najafi","doi":"10.5539/jmbr.v10n1p37","DOIUrl":"https://doi.org/10.5539/jmbr.v10n1p37","url":null,"abstract":"Background: Nursing is considered as one of the most stressful jobs due to the emotional nature of the patient's demands, long working hours, professionals and interpersonal conflicts. \u0000 \u0000Aim: The purpose of present research was the study of job satisfaction predictors consisted personality traits, resilience, emotional expression and ambiguity tolerance of emergency nurses in Tehran hospitals. \u0000 \u0000Methods: This is a descriptive and correlational study. The population includes all emergency nurses in all hospitals of Tehran in 2017. The sample size consisted of 300 nurses who were selected randomly. For data collection were used NEO Personality Inventory, Berkeley Emotional Expression Questionnaire (BEQ), Job Satisfaction Survey (JSS), Resilience Scale (RS) and Ambiguity Tolerance Scale (ATS). \u0000 \u0000Results: The results demonstrate that ambiguity tolerance, resilience and emotional expression respectively had respectively the highest impact on job satisfaction, but personality traits had the lowest impact on it. \u0000 \u0000Implications for Practice: Personality traits can be a predictor of the job satisfaction of emergency nurses in hospital environment.","PeriodicalId":92078,"journal":{"name":"Journal of molecular biology research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2020-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46523326","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Reviewer Acknowledgements for Journal of Molecular Biology Research, Vol. 9, No. 1","authors":"Grace Brown","doi":"10.5539/jmbr.v9n1p179","DOIUrl":"https://doi.org/10.5539/jmbr.v9n1p179","url":null,"abstract":"Reviewer Acknowledgements for Journal of Molecular Biology Research, Vol. 9, No. 1, 2019","PeriodicalId":92078,"journal":{"name":"Journal of molecular biology research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-11-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44418278","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genetic Basis of Giant Embryo Traits and Effects of Environmental Factors on Giant Embryo Rice","authors":"Bo Peng, Kun Xu, Kun-Hong He, Dong-Ying Tang, Juan Peng, Xiayu Tian, Yanfang Sun, Xiao-Hua Song, Lu-Lu He, Rui-hua Pang, Jintao Li, Quanxiu Wang, Wei Zhou, Huilong Li, Hongyu Yuan, A. Xin-Xiang","doi":"10.5539/jmbr.v9n1p149","DOIUrl":"https://doi.org/10.5539/jmbr.v9n1p149","url":null,"abstract":"Giant embryo rice (GMR) is a special rice which can produce eutrophic functional rice. Giant embryo rice and its intensive processing products have been widely used in food, medicine, health care products and other fields, with extremely important scientific significance and economic value. In recent years, a series of important advances have been made in the research of giant embryo rice, and its achievements have attracted the attention of rice breeders and consumers at home and abroad. In this paper, the genetic basis of giant embryo traits and the effects of environmental factors on giant embryo rice were reviewed and analyzed, and the application prospect of giant embryo rice was also prospected, these will provide important reference for genetic improvement and application promotion of giant embryo rice.","PeriodicalId":92078,"journal":{"name":"Journal of molecular biology research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49150006","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Epidemiological Study of Cutaneous Malignant Melanoma in Shiraz, Southwest of Iran between 2011 and 2016","authors":"Elaheh Shaghaghian, M. Namazi, Alireza Shaghaghian","doi":"10.5539/jmbr.v9n1p106","DOIUrl":"https://doi.org/10.5539/jmbr.v9n1p106","url":null,"abstract":"Background: Melanoma is the most deadly of all skin cancers in the world, its incidence rate has increased in the last decades. We aim to define the frequency, and epidemiologic features of Cutaneous Malignant Melanoma Cases diagnosed between 2011 and 2016 in teaching hospitals of Shiraz University of Medical Sciences, southwest of Iran. \u0000 \u0000Materials and Methods: This descriptive cross-sectional study was have performed at hospitals affiliated with Shiraz University of Medical Sciences from 2011 to 2016. Pathology reports collected from the laboratory along with general information such as age, sex, site of the tumor and had ulceration or not. SPSS version 23 statistical software was used for data analyzing. P-values less than 0.05 were considered significant. \u0000 \u0000Results: A total of 183 cases of Cutaneous Malignant Melanoma were registered in Faghihi Teaching hospital, 7 cases in Namazi Teaching Hospital and 6 cases in Motahari clinic in Shiraz, between 2011 and 2016. The average age was 64.5 years with age range of 1year to 89 years, mostly in women (51.5%) and also most of the tumor sites were in the foot 46 (23.5%) and then in the scalp 20 (10.2%). The incidence of ulceration was 41 (20.9%), and the most common stage of the tumor was stage one. \u0000 \u0000Conclusion: The results proved the importance of awareness of the physicians about the frequency and epidemiologic features of Cutaneous Malignant Melanoma in their region that they can diagnose or screen and treat them more earlier in better ways.","PeriodicalId":92078,"journal":{"name":"Journal of molecular biology research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-10-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70799207","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Investigating the Success of Cover Flap in Patients with Bedsore","authors":"S. Mortazavi, G. Amirian, M. Heidari","doi":"10.5539/jmbr.v9n1p119","DOIUrl":"https://doi.org/10.5539/jmbr.v9n1p119","url":null,"abstract":"Objective: The high prevalence of bedsore creates many problems for the maintenance system and the patients, and in addition to spending high costs for treating ulcers, a lot of time is also dedicated to caring for them. The purpose of performing this study is to investigate the therapeutic results of recovering bedsore injuries by cover flaps. \u0000 \u0000Work Method: This study is of prospective type in which 85 patients with bedsore who had referred to Taleghani Hospital in Kermanshah for treatment by muscle cover flaps during the years of 2016 to 2017, were followed up at time periods of 1 week, and 3 months after the discharge and in case of failure, they were recovered. Finally, the obtained data were analyzed by using statistical tests and SPSS version 22 software. \u0000 \u0000Results: The obtained results showed that the success percentage of recovering bedsores was significantly increased after one week, and 3 months by cover flaps (P <0.05). Also, the success percentage of recovering bedsores by cover flap after one week, and 3 months in terms of age, gender, and BMI of patients significantly shows an increase (P <0.05). \u0000 \u0000Conclusion: In general, it can be concluded from this study that using cover flaps leads to the success of recovering bedsores after 3 months of treatment, and the age, gender, and BMI variables of patients cannot be effective in this improvement process.","PeriodicalId":92078,"journal":{"name":"Journal of molecular biology research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-10-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45627083","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dental Caries among Tasters and Non-Tasters of PTC Substance in Students of Qurna Population","authors":"Hasna Amir Mohaus","doi":"10.5539/jmbr.v9n1p82","DOIUrl":"https://doi.org/10.5539/jmbr.v9n1p82","url":null,"abstract":"Objective: Dental caries is one of the most common diseases found in human populations and it is still prevalent and widespread in all around the world. newer strategies emphasize disease prevention as a model of disease management. For early detection and monitoring of caries rather than treatment Taste has valuable roles in our lives in health and disease. genetic markers such as PTC substance taste ability may represent a useful tool to predict the susceptibility to caries. This study aims to investigate the relationship between the taste ability of phenylthiocarbamide (PTC) substance and dental caries. \u0000 \u0000Materials and Methods: The current study was conducted from November 2017 to April 2018 in Qurna and Madiana population/Basrah/Iraq, PTC taste sensitivity was determined among random sample of (406) student from primary and secondary schools; 216 males and 190 females, their ages ranging from (6-17) years old. For dental caries assessment, dmft/DMFT scores were recorded. Individuals were divided into three groups (low, high and very high) according to their dental caries severity in addition to the free caries individuals. \u0000 \u0000Results: The proportion of tasters were higher as compared to non-tasters in this sample. The results showed an increase in the prevalence of caries among primary(68%) and secondary (57.6%) school’s students respectively and for both of them (64.03%). In primary school; females had an elevated percentage of caries (73.1%) compared to their counterpart males (65%). The results of this study did not show a significant relationship between the taste of PTC substance and dental caries, with equal proportions of caries prevalence among tasters and non-tasters (P > 0.05). However, the results showed significant differences in age effect in the prevalence of dental caries among primary and secondary students as it increased in individuals of the age group (6-12) years compared to the age group (13-17) years. \u0000 \u0000Conclusion: The results of this study did not show a significant relationship between the taste of PTC substance and dental caries. In other hand age have a significant influence especially in primary age school and females were more affected than males. The appearance of the two phenotypes of PTC taste may be due to the involvement of other genes, rather than the PTC gene only, including CA6 gene and its variation , which in turn affects the pH capacity of the saliva, a factor associated with tooth decay. Due to the multifactorial nature of dental caries, the individual’s vulnerability may differ from one to another. In addition, particularly regarding diet, part of the strategy to be adopted can be defined as health promotion by preventing the occurrence of the disease rather than treatment. Integrating a healthy dietary regimen into the daily routine can have a real influence on oral health and can be regarded as a primary step towards caries prevention. future research efforts should continue to emphasize early dete","PeriodicalId":92078,"journal":{"name":"Journal of molecular biology research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-10-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47439345","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Zeolite as a Bone Bio-Modifier Carrier: An In-Vitro Study","authors":"M. Mousavi, A. E. Nejad, E. Shamsoddin, M. M. Golabgiran, B. Houshmand","doi":"10.5539/jmbr.v9n1p126","DOIUrl":"https://doi.org/10.5539/jmbr.v9n1p126","url":null,"abstract":"Background: Zeolite is a microporous aluminosilicate compound which has been successfully used in tissue engineering. The effects of Zeolite on the morphology and functions of pre-osteoblastic MG-63 cells as new bone enhancer material is still unclear. \u0000 \u0000Methods: In this vitro experimental study, MTT and Alizarin red staining test were performed on six groups of MG-63 cells which differed in Zeolite (Z) concentration and the presence or absence of Alloplast extract (A). Group A: 0.1μg/mL Z+A, Group B: 0.1μg/mL Z without A, Group C: 0.2μg/mL Z+A, Group D: 0.2μg/mL Z without A, Group E: 0.3μg/mL Z+A, Group F: 0.3μg/mL Z without A. There were also three control groups as positive control, negative control, and Alloplast control based on each related test. The data were analyzed by SPSS 20 via one-way ANOVA and Welch test. (P<0.05). \u0000 \u0000Results: At 24 hours, results showed that solutions with 0.1μg/mL, 0.2μg/mL, and 0.3μg/mL Zeolite with or without Alloplast had significantly higher proliferation rates than positive control (distilled water) groups without Alloplast (p<0.001). At 72hours time point, the results showed significantly higher proliferation rates in the solutions with 0.1μg/mL, 0.2μg/mL, and 0.3μg/mL Zeolite with or without Alloplast compared to the positive control group without Alloplast (p<0.001). \u0000 \u0000Conclusions: Zeolite can increase proliferation of MG-63 cells without presence of Alloplast; It seems that combination of Zeolite with Alloplast maybe enhancing proliferation and function of MG-63 cells.","PeriodicalId":92078,"journal":{"name":"Journal of molecular biology research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-10-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46426044","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}