Journal of molecular biology research最新文献

{"title":"An Interesting Case Report of Frontal Sinus Keratocyst","authors":"A. Mohebbi, Mohammad Aghajanpour","doi":"10.5539/jmbr.v9n1p100","DOIUrl":"https://doi.org/10.5539/jmbr.v9n1p100","url":null,"abstract":"Purpose: To report an unusual case of frontal sinus keratocyst. \u0000 \u0000Case Report: A 31 year old woman presented with a history of swollen right eye and visual field impairment. The ophthalmological examination revealed right superior eyelid edema and some degrees of proptosis. CT scan with iodine injection evidenced the opacification of the right frontal sinus with superior orbital wall lysis. Complete endoscopic surgical resection of a mass containing foul smelling keratin material was performed via Draf type III. The patient became symptomatic after 6 years. Imaging studies showed neo- osteogenesis in the far lateral end of right frontal sinus. Frontal trephination was done and the remnant of keratocyst was removed and the septum interfacing the cell and the right frontal sinus was walled down entirely. \u0000 \u0000Conclusion: Although frontal sinus keratocyst is benign but can spread to the surrounding structures leading to several complications. Therefore, complete surgical resection is necessary to avoid recurrence.","PeriodicalId":92078,"journal":{"name":"Journal of molecular biology research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-10-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46570758","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of The Improvement of Clinical and Radiological Scores with Doxycycline in the Treatment of Nasal Polyposis: A Randomized Double Blind Clinical Trial","authors":"A. Mohebbi, Mohammad Aghajanpour, H. Ghanbari, Zhila Mohammadi Azar, Ahmad Alaghehband, F. Safdarian","doi":"10.5539/jmbr.v9n1p142","DOIUrl":"https://doi.org/10.5539/jmbr.v9n1p142","url":null,"abstract":"Although several trials have been proposed for nasal polyposis (NP) treatment, but no standard guideline has been declared yet. We evaluated the effect of doxycycline on NP treatment. In a double- blind randomized clinical trial, 48 patients with bilateral nasal polyposis were assigned to receive doxycycline and placebo capsules for 8 weeks and were followed monthly for 4 months. Patients were assessed for polyp size, severity of symptoms and quality of life according to the endoscopic examination, VAS score, CT scanning and SNOT-22 respectively. polyp size reduced significantly after 8 weeks of treatment in the drug group. The quality of life score has raised significantly after 2 months of treatment with doxycycline. By giving –up the drug, severity of symptoms started to grow-up after 2 months. Although a course of doxycycline improved nasal symptoms, polyp size and CT findings, but further studies with more patients and longer follow-up course are required to establish the foundation to recommend this drug as an important treatment in NP.","PeriodicalId":92078,"journal":{"name":"Journal of molecular biology research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-10-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41454110","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Association between Functional Polymorphisms of COX-2 and Serum PGE2 Level in ESCC Patients in North of Iran","authors":"M. S. Sheshpoli, Safoura Khajeniazie, Masoud Khoshnia, N. Behnampour, M. Saeedi, A. Moradi","doi":"10.5539/jmbr.v9n1p133","DOIUrl":"https://doi.org/10.5539/jmbr.v9n1p133","url":null,"abstract":"Background: Esophageal cancer is recognized as one of the most fatal diseases around the world. Many factors are involved in the development of esophageal cancer, including genetic factors and inflammation. Cyclooxygenase-2 (COX-2) and its downstream signaling are the most important proinflammatory factors contributing to cancer. The present study aimed to evaluate the relationship between the polymorphisms and expression of COX-2 and prostaglandin-E2 (PGE2) level in patients with esophageal squamous cell carcinoma (ESCC) in Golestan Province (Iran), situated on the “esophageal cancer belt”. \u0000 \u0000Methods: In this case-control study, blood and biopsy samples were obtained from ESCC patients and healthy controls. The COX-2 polymorphisms for -1195, -1290, -765, and +8473 SNPs were assayed using PCR-RFLP assay, while the level of PGE2 was measured using an ELISA kit. In addition, real-time PCR assay and immunohistochemistry (IHC) were performed to assay mRNA and protein expression of COX-2, respectively. \u0000 \u0000Results: An association was found between 8473TC genotype and risk of ESCC (OR= 5.417, P= 0.036). In addition, mRNA and protein expression of COX-2 in ESCC patients was higher than the controls (P=0.001 and P=0.048, respectively). Based on the findings, the level of PGE-2 was significantly higher in ESCC patients, compared to the controls (P= 0.045). However, ROC curve analysis revealed PGE2 is a weak biomarker for diagnosis of ESCC. There was a significant relationship between the level of PGE2 and 8473CC, 8473TC, -765CC, and -1290AA genotypes (P= 0.028, P= 0.022, P= 0.024, and P= 0.011, respectively). \u0000 \u0000Conclusion: Based on our results, functional polymorphisms of COX-2 (8473CC, 8473TC, - 765CC, and -1290AA) increase PGE2 level and carriers of these polymorphisms might be more susceptible to ESCC.","PeriodicalId":92078,"journal":{"name":"Journal of molecular biology research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-10-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42832418","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Association between Severity of Low Back Pain (LBP) and Magnetic Resonance Imaging Pathologies in Patients with Acute LBP","authors":"Mohammadreza Mahabadi Mahabad, B. Sartip","doi":"10.5539/jmbr.v9n1p93","DOIUrl":"https://doi.org/10.5539/jmbr.v9n1p93","url":null,"abstract":"Background and Objectives: Low Back Pain (LBP) is a common health problem that affects people worldwide, and about 15% of Iranians. It imposes high costs to societies and requires great attention. Magnetic Resonance Imaging (MRI) is considered a reliable and accurate diagnostic tool, however some studies have questioned the appropriateness of MRI in LBP. Thus, this study aimed to evaluate the relationship between the intensity of LBP and positive findings in MRI, in patients with acute LBP. \u0000 \u0000Materials and Methods: A cross-sectional study was performed on all patients, who reported less than four weeks of LBP and were referred to the radiology center of Shafa-e-Yahyaeian hospital, ranged from March to July, 2014, to perform MRI. Data collected included demographics and pain characteristics, in addition to an Oswestry Disability Index questionnaire. MRI pathologies were distinguished by two spinal surgeons and one radiologist. Data was analyzed using the SPSS software. \u0000 \u0000Results: A total of 200 patients were enrolled in this study with a mean age of 41.78 years. Nearly half (48%) of the studied patients had positive MRI findings on L4-L5, and NFS and disc bulging (42.5%, and 38%, respectively) were the most common pathologies, while 11.5% of patients had normal MRIs. The mean ODI was highest in patients with vertebral body fracture, and disc herniation and lowest in hemangioma. \u0000 \u0000Conclusion: A noticeable percentage of patients demonstrated a high ODI score, but MRI findings were in significant association with age of patients, indicating that patients with acute LBP do not require MRI immediately. Using the ODI questionnaire can help us towards a better diagnostic approach.","PeriodicalId":92078,"journal":{"name":"Journal of molecular biology research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-10-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49529570","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Anti-Proliferative and Apoptotic Activity of Rumex obtusifolius seed’s Hydroalcoholicextract on Human Colon Cancer Cell Line “SW480”","authors":"Ladan Badeei Pourtaghi, F. Bineshian, B. S. Moghadam, R. Ghorbani, H. Nazari","doi":"10.5539/jmbr.v9n1p111","DOIUrl":"https://doi.org/10.5539/jmbr.v9n1p111","url":null,"abstract":"Cancer is considered a serious threat to human health thatcolon cancer is the third most common cancer in the world. Colon cancer has undergone significant growth worldwide in recent years, and many people die every year as a result of this disease, which its treatment is a major challenge for researchers. In this study, we investigated the effect of anti-proliferative and apoptotic activity seed’s hydroalcoholic extract of Rumex obtusifolius, “is native to Semnan's northern region” on the human colon cancer cell line, SW480. \u0000 \u0000The SW480 human colon cancer cell line were cultured in RPMI 1640 cell culture containing 10% Fetal bovine Serum and 1% Penicillin and Streptomycin antibiotics and incubated with a relative humidity of 95%, carbon dioxide 5% at 37°C. The SW480 cancer cell line was cultured in 48 and 96 well plates and treated with different concentrations of seeds hydroalcoholic extract of Rumex obtusifolius (20/40/80/160/320 micrograms/ml) for evaluation of cell viability by Trypan blue exclusion method and MTT assay. A non-treated group was used as control and a treated group with DMSO was used as solvent in solution of powdered extract as vehicle as well as positive control groups. Also, cell apoptosis assessment was performed by Propideum iodide/Annexin V test “Flowcytometry”and DNA fragmentation analysis “Agaros electrophoresis”. \u0000 \u0000Seed hydroalcoholic extract caused cell death at concentrations of 40, 80, 160 and 320μg/ml, which the anticancer effect trend to increase significantly from 80μg/ml. The effect of R. obtusifolius seed extract even has more anti-cancer effect in 160 and 320μg/ml than cells which is treated to 5FU as a positive control groups. \u0000 \u0000Seed hydroalcoholic extract reduced cell viability in proportion concentration. The effect of anticancer activity of seed extract trend to increase from 40μg/ml but it was shown effective concentrations for inhibiting of the cells growth were 160, 320μg/ml in 48 hr incubation time and 80, 160 and 320μg/ml in 72 hr incubation time. Also, the effect of this extract at concentrations of 160 and 320μg/ml with control group is not significant which have more cytotoxicity and even extract can have a significant effect on decreasing the survival of the SW480 cells. The data generated by flow cytometry are shown that colon cancer cells trend to necrosis and apoptotic pathway from 40μg/ml and increased significantly entrance to apoptotic pathway from 80μg/ml which is confirm the Trypan blue and MTT assay tests. Since the appearance of the DNA fragmentation, which precedes the bioavailability change, is characteristic to apoptosis, the extracts were considered to be apoptosis-inducers. The ability to inhibit growth and induction of apoptosis in SW480 cancer cells is a concentration- and time dependent manner. These results indicated that the induction of apoptosis at least partly mediates their cytotoxic activity which is necessary down further study for elucidation apoptotic signaling path","PeriodicalId":92078,"journal":{"name":"Journal of molecular biology research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-10-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45959727","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mineral Composition of Sapropelles of Lakes of the Right Bank of the Ob River (Middle Ob Region)","authors":"M. Sartakov, E. Osnitsky, K. Kudrin, N. S. Larina","doi":"10.5539/jmbr.v9n1p77","DOIUrl":"https://doi.org/10.5539/jmbr.v9n1p77","url":null,"abstract":"The objective of this work is to study the mineral composition of bottom sediments of eight small lakes located on the right bank of the Ob River (Western Siberia) in the Surgut region of the Khanty-Mansiysk Autonomous Okrug - Yugra. The studies were carried out using wave dispersive X-ray fluorescence (WDFR) spectroscopy. The content of organic substances, ash, and oxides in the ash in the samples was determined. Based on these data, the studied bottom sediments were classified. The bottom sediments of Lake S-1 are siltstone sands, lakes Vach Lor and S-189 - weakly sapropelic siltstone sands, lakes S-5, S-6, S-3, S-89, and S-294 - typical sapropels. The article presents the content of the main elements in the ash of the studied samples.","PeriodicalId":92078,"journal":{"name":"Journal of molecular biology research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-10-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41831234","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Penetrating Keratoplasty with a Preserved Donor Cornea in Veterinary Ophthalmology","authors":"B. Usha, S. Kontsevaya, V. Lutsay, I. M. Nityaga, Uliyana E. Lukashina","doi":"10.5539/jmbr.v9n1p71","DOIUrl":"https://doi.org/10.5539/jmbr.v9n1p71","url":null,"abstract":"The present article describes the penetrating keratoplasty using the technique of rehydration of donor cornea dehydrated over silica gel, as well as complications that can arise. The indications included corneal ulcers, descemetocoele, corneal sequestrum and corneal perforation. 37 (88.37%) out of 43 corneal transplants had a favorable outcome in surgeries with complete excision of abnormal tissues. In 5 cases (11.63%), the authors faced such complications as suture failure, formation of anterior synechias, incomplete epithelization after the suture removal, and transplant swelling reversed with the preservation of eye optical functions, and secondary glaucoma in the late postoperative period that ended up in eyeball endoprosthesis transplantation. Due to a high rate of efficiency of the proposed method, penetrating keratoplasty with rehydrated grafts can be recommended for the treatment in case of medical emergency experienced by cats and dogs.","PeriodicalId":92078,"journal":{"name":"Journal of molecular biology research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-10-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49198873","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of Fractional CO2 Laser Treatment Efficacy and Comparison to Vaginal Conjugated Estrogen Cream in Postmenopausal Women with Vulvovaginal Atrophy: A Randomized Clinical Trial","authors":"M. Najafian, K. Shojaei, Saadat Hajatzadeh","doi":"10.5539/jmbr.v9n1p67","DOIUrl":"https://doi.org/10.5539/jmbr.v9n1p67","url":null,"abstract":"Background: Vulvovaginal atrophy is common and bothersome among postmenopausal women. Hence in this study, the fractional CO2 laser treatment efficacy was compared with vaginal conjugated estrogen cream in postmenopausal women with vulvovaginal atrophy was assessed. \u0000 \u0000Materials and Methods: In this randomized clinical trial, 130 consecutive postmenopausal women with vulvovaginal atrophy attending to urogynecologic clinic in Imam-Khomeini hospital in Ahvaz in 2015 were enrolled and were randomly assigned to receive either fractional CO2 laser treatment or vaginal conjugated estrogen cream. The improvement of vulvovaginal atrophy symptoms, sexual satisfaction and function were compared across the groups after 12 weeks. \u0000 \u0000Results: There improvement of vulvovaginal atrophy symptoms, sexual satisfaction, and function were 86.2%, 87.7%, and 87.7%, respectively in laser group and 53.8%, 52.3%, and 52.3%, respectively in primarin group showing statistically significant differences (P=0.0001). There were no side effects. \u0000 \u0000Conclusion: Totally, according to obtained results, it may be concluded that efficacy of fractional CO2 laser was higher than vaginal conjugated estrogen cream in postmenopausal women with vulvovaginal atrophy.","PeriodicalId":92078,"journal":{"name":"Journal of molecular biology research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-07-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45812799","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Immunophenotype Study in Children with Leukemia and Its Relationship with Relapse in Ahvaz Shafa Hospital from 2013 to 2018","authors":"Arash Alghasi, Jamshid Safdarian","doi":"10.5539/JMBR.V9N1P61","DOIUrl":"https://doi.org/10.5539/JMBR.V9N1P61","url":null,"abstract":"Introduction: Acute lymphoblastic leukemia (ALL) is one of the most common cancers in children. Since the identification of patients immunophenotypes is effective for disease diagnose and prognosis, and due to very few studies in Iran, especially Khuzestan province, the aim of this study was to investigate the immunophenotype of children with leukemia and its association with recurrence in Ahvaz Shafa Hospital from 2013 to 2019. \u0000 \u0000Methods: This is a retrospective study that was conducted in patients referring to Ahvaz Shafa Hospital during the years 2013 to 2019. Demographic data including age, gender, height, weight, leukocyte count, flow cytometry and other laboratory findings were collected and analyzed by version 22 SPSS statistical software. \u0000 \u0000Results: The mean age of the patients was 7.59 ± 3.94 years and the sex of 51 (52%) were female. Immunophenotype of 81 patients (82.7%) was Pre B cell, 4 patients (4.1%) was Pro-B Cell, 5 patients (5.1%) was Pro-T Cell, and 8 patients (8.2%) was T Cells. 87 patients (88.8%) recovered and 11 patients (11.2%) had recurrence. Only 11 patients with Pre B cell (13.6%) had recurrence, but in other immunophenotypes, 100% of patients had remission, but this difference was not statistically significant (p = 0.46). There was no statistically significant difference between the prevalence of phenotypes in female and male sexes (p = 0.76). The incidence of recurrence was 9.8% in female patients and 12.8% in male patients, which was not statistically significant (p = 0.64). \u0000 \u0000Conclusion: The results of this study showed that preB cell immunophenotypes with the prevalence of 82.7% had the highest frequency in acute lymphoblastic leukemia, and the incidence of recurrence in patients was 11.2%. Also, patients with other immunophenotypes did not recurrence, so his finding may have been because of insufficent patients to study and compare.","PeriodicalId":92078,"journal":{"name":"Journal of molecular biology research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-06-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42852505","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Application of the 3'-Untranslated Region of Messenger RNA from Measles Virus Matrix Protein as an RNA Stabilizer: Implications in Pharmaceutical Biotechnology","authors":"Marzieh Marzbany, F. Ghassemi, M. Rasekhian","doi":"10.5539/JMBR.V9N1P53","DOIUrl":"https://doi.org/10.5539/JMBR.V9N1P53","url":null,"abstract":"BACKGROUND: The market for the use of recombinant proteins for medical applications has been increasing in recent years. In many cases including fast production of significant amounts of protein for research purposes, transient transfection is the method of choice. In this regard expression vectors are one of the decisive factors in the cost-effectiveness of the production process. The genetic elements found in the 3’untranslated region (UTR) of mRNA expressed by such vectors, play an essential role in determining its stability and thus in the efficiency of the process. \u0000 \u0000METHODS: In this study, the 3'UTR of matrix protein from the Measles Virus (MV) was used to construct a reporter plasmid containing Enhanced Green Fleurocent Protein (EGFP). The reporter construct was transfected into three cell lines. The effect of 3'UTR on mRNA stability was evaluated by real-time PCR. Secondary structure of the mrna was predicted based on minimum free energy. 3'UTR was analyzed in silico for the presence of binding motifs for trans-acting elements with known effects on RNA stability. \u0000 \u0000RESULTS: Addition of 3’UTR of MV matrix protein sequence to the 3’ end of the mRNA, increased the EGFP- mRNA stability in time and cell-dependent manner. Analysis for the presence of known cis-acting motifs in 3’UTR indicated the presence of two PABPC1 binding sites, an RNA-binding protein, known for its stability and translation enhancing effects. \u0000 \u0000CONCLUSION: Our results verified the potential of the 3'UTR region of matrix protein mRNA for improvement of transient recombinant protein production and vector design for mammalian cell hosts.","PeriodicalId":92078,"journal":{"name":"Journal of molecular biology research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41494030","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}