Journal of clinical and laboratory medicine最新文献

{"title":"Comparative Assessment of Serum versus Menstrual Blood for Diagnostic Purposes: A Pilot Study","authors":"Naseri S, Lerma K, Blumenthal Pd","doi":"10.16966/2572-9578.130","DOIUrl":"https://doi.org/10.16966/2572-9578.130","url":null,"abstract":"Background: Blood testing remains an essential component of diagnosis and prevention of disease. Phlebotomists, Laboratory Infrastructure, Storage, Collection, and Transport are required for Current Methods of Blood Collection. Menstrual Blood has many similar characteristics to circulating blood but has not previously been assessed as a Potential Diagnostic Resource. Objective: To assess if biomarkers derived from menstrual blood correlate with systemic blood. Study design: This was a prospective, observational pilot study of healthy reproductive-aged women. We chose a panel of 9 Biomarkers, used in preventative health assessments and for following clinical conditions, and compared systemic and menstrual blood levels. Results: Eighty-Four volunteers were screened over two months; 35 provided a menstrual and serum sample, of which 20 had a sample adequate for analysis. Overall, the correlation was observed, particularly for 7 Biomarkers, with no statistically significant differences between the mean menstrual and serum values. These include cholesterol (P for differences in means=0.89, R2 for correlation=0.89), Creatinine (P=0.32, R2=0.94), HSCRP (P=0.89, R2=0.99), LDL (P=0.21, R2=0.84), Triglycerides (P=0.45, R2=0.89), Hba1c (P=0.54, R2=0.80), and HDL (P=0.33, R2=0.77). One biomarker, FSH (P<0.001, R2=0.97), was less directly comparable to systemic blood, but a linear relationship was recognized suggesting that the correlation could be mathematically derived, and therefore diagnostic utility is possible. Conclusion: Based on our results, menstrual blood can reliably estimate levels of several biomarkers and may be a promising option for noninvasive collection of blood for diagnosis and health monitoring. Larger trials are needed to confirm these findings. of disorders were present in menstrual fluid [1]. These disorders include endometriosis, breast, Cervical, Ovarian and Endometrial Cancer. Several other studies have detected the presence of Human Papilloma Virus (HPV) in menstrual blood implying that it may be useful for non-invasive screening for cervical cancer or pre-cancer [2,3]. Furthermore, menstrual blood has been studied for screening or diagnosis of Sexually Transmitted Infections (STIS). Alary M, et al. explored the potential of using vaginal fluid collected in menstrual pads for Chlamydia detection. This proved to have sensitivity and specificity equivalent to, or even higher than, existing methods, including vaginal swabs or urine samples [4]. These data combine to provide support for the potential utility of menstrual blood-based testing as a non-invasive alternative blood source for diagnostic or therapeutic analysis. Despite this apparent potential, menstrual blood remains a relatively uninvestigated area for diagnostics; no published literature explores the correlation of systemic versus menstrual-based blood for assessing biomarkers relative to indicators of health or disease. Introduction Whole blood or Serum (Systemic Blood)","PeriodicalId":92069,"journal":{"name":"Journal of clinical and laboratory medicine","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67394447","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Castleman’s Disease about a Case","authors":"D. Ap, Pazos Cp, Penton Crc, D. Ap, Perez Lr, L DominguezPlain","doi":"10.16966/2572-9578.131","DOIUrl":"https://doi.org/10.16966/2572-9578.131","url":null,"abstract":"Castleman’s disease is a very rare lymphoproliferative disorder. There are two Clinical forms: the unicentric, which is usually benign, and the multicentric progressive course. There are three described histological patterns: hyali neovascular of unicentric presentation, of plasma cells of multicentric presentation and the mixed one. The aim of this paper is to present a clinical case of multicentric Castleman’s disease of hyaline vascular histology very little described in the literature. The patient started with asthenia, anorexia, profuse nocturnal sweating, weight loss, generalized lymphadenopathy, splenomegaly and respiratory episodes. At physical examination: multiple cervical, axillary and bilateral inguinal adenopathies. The diagnosis was made with the histopathological study of the resected ganglion, the evolution after the treatment has been favorable. Because there are no specific clinical and radiographic features, a high degree of diagnostic suspicion and the experience of the pathologist are required to diagnose this rare disease.","PeriodicalId":92069,"journal":{"name":"Journal of clinical and laboratory medicine","volume":"286 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67394630","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Electrogastrogram Analysis in Children with Chronic Abdominal Pain","authors":"Yu Lp, C. Jie, Zhou Ql, G. Ming","doi":"10.16966/2572-9578.125","DOIUrl":"https://doi.org/10.16966/2572-9578.125","url":null,"abstract":"Objective: Electrogastrography (EGG) is a non-invasive method for the diagnosis of gastric electrical activity. This study used an EGG to analyze the gastric activity in children with Chronic Abdominal Pain (CAP). Methods: We conducted a retrospective analysis of patients with CAP who underwent EGG at the first affiliated Hospital of Anhui Medical University. Multichannel EGG with 8-minutes recordings preprandially and postprandially was done in children with CAP. Computerized spectral analysis methods were used to compute various EGG parameters. Results: Chronic superficial gastritis and Functional Dyspepsia (FD) were very common among 96 patients with CAP. The dominant power and waveform response area of EGG was significantly lower in FD patients and chronic superficial gastritis in both preprandial and postprandial states. DP, WRA and power ratio was obviously higher in patients with superficial gastritis, duodenitis and reflux esophagitis in their postprandial states. Conclusions: Gastric myoelectrical activity abnormalities were observed in CAP patients and different abnormality of EGG parameters was shown in different groups. EGG is an effective, reliable and non-invasive method in the diagnosis of CAP subgroups.","PeriodicalId":92069,"journal":{"name":"Journal of clinical and laboratory medicine","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67394190","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessment of Lipid Profile Pattern in Patients with Hyperthyroidism in a Tertiary Hospital in Kano, North Western Nigeria","authors":"Yahaya Is, Adimabua Op, Ocheni Ap, Adamu Gb","doi":"10.16966/2572-9578.127","DOIUrl":"https://doi.org/10.16966/2572-9578.127","url":null,"abstract":"Background : Hyperthyroidism is an important metabolic disorder associated with multiple biochemical abnormalities that have direct impact on lipid metabolism. The aim of the current study is to assess lipid profile pattern in patients with hyperthyroidism attending Aminu Kano Teaching Hospital, Kano. Methods : A case control study conducted during the period of November 2015 to December 2017 among 100 hyperthyroidism patients before treatment and 100 apparently healthy individuals as controls with age between 18-70 years. Sixty eight of the patients were females while thirty two were males. Fasting blood was collected from each individual to determine serum TSH, T3 and T4 with quantitative ELISA assay technique, Product of ACCU BIND Inc., USA. Lipid profile was measured by an enzymatic spectrophotometric method and precipitation enzymatic method for HDL-C. SPSS software package version 20 was used for the analysis of data. Results : The results of the present study showed that the mean values of T3, T4, TG and TG/HDL-C ratio were significantly higher (p<0.05) in patients with hyperthyroidism than the control group while TSH, T4/T3 ratio TC, HDL-C and LDL-C were significantly lower (p<0.05) in patients with hyperthyroidism than the control group. There were significant (p<0.05) and positive correlations between TSH and TC, HDL-C and LDL-C respectively in the patients. There was no significant (p<0.05) correlation between TSH and TG in the patients. There were no significant correlations in the control group of the respective analytes. Conclusion : Our results revealed that hyperthyroidism was associated with decrease total cholesterol, high density lipoprotein cholesterol, low density lipoprotein cholesterol and slightly increased triglycerides. LDL and negative correlation, but not significant between TSH a pond TGs. These findings are similar with the report of Shashi A and Sharma N [31]. They reported positive correlations between serum TSH and TC, TSH and LDL, TSH and HDL, but not with TSH and TG. On the other positive correlations changes in resting subsequent loss of disparities findings compared previous findings differences in the research design and the subjects used in the studies.","PeriodicalId":92069,"journal":{"name":"Journal of clinical and laboratory medicine","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67394503","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of Multiple Freeze/Thaw Cycles on Measurements of Potential Novel Biomarkers Associated With Adverse Pregnancy Outcomes.","authors":"Sandra L Rebholz,&nbsp;John T Melchior,&nbsp;Jeffrey A Welge,&nbsp;Alan T Remaley,&nbsp;W Sean Davidson,&nbsp;Laura A Woollett","doi":"10.16966/2572-9578.107","DOIUrl":"https://doi.org/10.16966/2572-9578.107","url":null,"abstract":"<p><p>World-wide, millions of women enter preterm labor or have small newborns. Effective biomarkers are needed to identify women at risk for these adverse outcomes. A time and cost effective way to examine any potentially new biomarkers in samples collected during prior studies or trials that had been assayed for other metabolites would be highly useful. Thus, the current study aimed to determine if samples that had been previously thawed and re-frozen could be re-assayed for novel biomarkers, those being lipoprotein composition (sizing, proteome, lipids) and combined cholesterol and cytokine concentrations. Fasting blood was collected from 51 young non-pregnant women and plasma was analyzed for lipoprotein composition and cytokine concentrations after multiple freeze/thaw cycles in the cold or at room temperature and after being stored for 18 months. Plasma LDL-C, HDL-C, total cholesterol, and triglyceride concentrations decreased <6-7% (cholesterols) or <20% (triglyceride) after 7 thaws in the cold, 3 thaws at room temperature, and after 18 months of storage. As these decreases were less than day-to-day reported variation of lipids, they do not appear to be physiologically significant. Cytokine (IL-6, TNF α, IL-8, IL-1β) and hsCRP concentrations decreased by 22%, 8%, 8%, 22%, and 35%, respectively; only IL-6, IL-1β and hsCRP concentrations showed significant decreases greater than day-to-day variations of 20%. For measured triglyceride and cytokine, but not cholesterol concentrations, decreases with freeze/thaw cycles were greater when concentrations were elevated. Multiple thaws also led to changes in lipoprotein sizing, specifically to a shift from medium- and large-sized HDL particles to small-sized HDL particles and from large LDL to IDL. No changes occurred for VLDL particle numbers. Though particle sizes changed, the HDL proteome did not change with multiple thaw cycles or after long term storage. Overall, the results demonstrate that it is possible to use previously obtained frozen samples for plasma cholesterol and triglyceride levels and the lipoprotein proteome, and lipoprotein sizing and cytokine concentrations if one knows the history of the sample as changes should be relative to one another.</p>","PeriodicalId":92069,"journal":{"name":"Journal of clinical and laboratory medicine","volume":"2 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5720390/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35241782","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Clinical and Laboratory Medicine-Sci Forschen Full Text RESEARCH ARTICLE Exocrine Pancreatic Cell NF-κB/AP-1 Crosstalk Downstream of ERK MAPK","authors":"Twait Ec, Samuel I","doi":"10.16966/2572-9578.137","DOIUrl":"https://doi.org/10.16966/2572-9578.137","url":null,"abstract":"Aim: To test the hypothesis that cooperative interactions between transcription factors Nuclear Factor-kappa B (NF-κB) and Activator Protein-1 (AP1) augment transcriptional activity in exocrine pancreatic cells.","PeriodicalId":92069,"journal":{"name":"Journal of clinical and laboratory medicine","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67394740","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}