Journal of clinical and laboratory medicine最新文献

{"title":"MicroRNAs: Emerging as Highly Promising Biomarkers for Early Breast Cancer Screening.","authors":"Karl Krupp, Jennifer M Segar, Juan Lewis Fernández-Martínez, Purnima Madhivanan","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":92069,"journal":{"name":"Journal of clinical and laboratory medicine","volume":"6 3","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10629926/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71491259","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Plasma Biomarkers Associated to Outcome in Patients with Sars-Cov-2 Infection","authors":"Venge P, Xu S, P. C., Eriksson Ak, H. M, Larsson A, Lipcsey M, Bulow S, F. R","doi":"10.16966/2572-9578.141","DOIUrl":"https://doi.org/10.16966/2572-9578.141","url":null,"abstract":"Background: A serious consequence of respiratory infections with SARS-CoV-2 is Acute Kidney Injury (AKI). The aim of this study was to evaluate some novel biomarkers measured in plasma for their associations with the outcome of this infection as to AKI and mortality. Methods: Four different HNL (Human Neutrophil Lipocalin) assays were constructed using different antibody configurations, reflecting both neutrophil and epithelial cell activities. TK-1 (Thymidine kinase-1) was measured as a sign of cell destruction and proliferation. For comparison the plasma concentrations of NGAL (Neutrophil Gelatinase Associated Lipocalin), the cytokines IL-6, IL-8 and IFN-γ and of cystatin C, and procalcitonin were also measured. One hundred and three patients with severe respiratory failure admitted to the ICU were sampled for blood at admittance and at day three (n=67) after admittance. Results: Sixty-five percent of the patients developed AKI. The performance of the HNL assay 763/8F was most closely associated to outcome as to AKI and mortality with Area Under the Receiver Operating Characteristics Curve (AUC) of 0.87 and a sensitivity of 80% and a specificity of 88% in COVID-19 patients with and without severe AKI (p<0.0001). TK-1 was significantly related to the development of AKI (p=0.01) and the three cytokines were related to mortality (p=0.004-p<0.001). Conclusion: The HNL variant 763/8F and TK-1 are novel biomarkers that might become useful and novel tools in the management of COVID-19 patients, but likely also in other patients affected by AKI.","PeriodicalId":92069,"journal":{"name":"Journal of clinical and laboratory medicine","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67394917","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Benign Cerebral Edema and Increased Intracranial Pressure (ICP) as Manifestations of COVID-19 Reinfection; A Case Report","authors":"Heidary Ah, Elahi R, N. M","doi":"10.16966/2572-9578.138","DOIUrl":"https://doi.org/10.16966/2572-9578.138","url":null,"abstract":"Reinfection with COVID-19 is of great importance since it can substantially affect the future of disease control and vaccine development. COVID-19 can present with diverse neurological, as well as respiratory and gastrointestinal symptoms. Here, we present a rare case of COVID-19 reinfection with neurological manifestations. The patient was a healthy 26-year-old male that had experienced first episode of COVID-19 infection with mild respiratory and gastrointestinal symptoms. In his second episode of infection, he presented to the emergency department with vertigo, vomiting, metamorphopsia, blurred vision, headache, and gate disturbance. Based on the clinical data and imaging studies, the patient was diagnosed with benign cerebral edema and intracranial hypertension. Then, he was treated using intravenous/intramuscular corticosteroids in addition to the supplementary therapies. His symptoms dramatically improved during the next 2 days. No important neurologic or systemic adverse events in response to the treatment were noted. This case reveals that infection with COVID-19, despite positive IgG, does not protect the patient from reinfection. Moreover, COVID-19 reinfection can present with benign cerebral edema and intracranial hypertension, in which case, inflammatory suppression therapy should be considered.","PeriodicalId":92069,"journal":{"name":"Journal of clinical and laboratory medicine","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67394781","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Serology Testing: The Dark Horse in SARS COV2 Pandemic","authors":"Rajneesh Sareen, Gupta Gn, A. Yadav, S. Saini","doi":"10.16966/2572-9578.136","DOIUrl":"https://doi.org/10.16966/2572-9578.136","url":null,"abstract":"Introduction: SARS COV-2 pandemic has been a nightmare for medical, political and social systems all over the world, right from the point of initial diagnosis, treatment protocols, anticipated vaccine development and prevention strategies.","PeriodicalId":92069,"journal":{"name":"Journal of clinical and laboratory medicine","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67394734","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nobiletin Reduces Lipid Accumulation in Sebocytes and Inhibits PPAR Delta Activation in Epidermal Tissue Models","authors":"E. Fedorova, S. Li, Gusella Gl, A. Mosoian","doi":"10.16966/2572-9578.139","DOIUrl":"https://doi.org/10.16966/2572-9578.139","url":null,"abstract":"Background: Acne vulgaris is a chronic inflammatory disease that affects the majority of the population at some point in their lifetime. Acne pathogenesis is multifactorial with four primary contributors that play a pivotal role in the formation of acne lesions: inflammation, androgeninduced sebum production, abnormal keratinization, and bacterial colonization. Recent studies have demonstrated the anti-inflammatory, anticarcinogenic, anti-diabetic, and anti-lipid properties of certain Polymethoxylated Flavones (PMF) derivatives.","PeriodicalId":92069,"journal":{"name":"Journal of clinical and laboratory medicine","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67394791","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Acute Erythroid Leukemia: A Rare Case Report","authors":"K. Akhtar, Sadaf Haiyat, A. Khan, B. Juneja, T. Khan, S. H. Arif","doi":"10.16966/2572-9578.133","DOIUrl":"https://doi.org/10.16966/2572-9578.133","url":null,"abstract":"AML with myelodysplasia related changes is an uncommon type of acute myeloid leukemia. It comprises less than 5% of acute leukemias. According to the recent World Health Organization (WHO-2016) classification, AML cases with ≥ 50% or more erythroid cells and ≥ 20% total myeloblasts should be diagnosed as AML with myelodysplasia-related changes. Morphologic cellular features help to establish the diagnosis. We present a rare case of AML with myelodysplasia related changes in a 35-year-old female who presented with low-grade fever, mild epistaxis, and shortness of breath and diffuse sternal tenderness. The peripheral smear showed features of pancytopenia with 6% blasts along with evidence of hemolysis. Bone marrow examination revealed erythroid hyperplasia with 65% erythroblasts and 24% myeloblasts. Flow cytometry was used for the confirmation of the diagnosis. The patient was administered chemotherapy with Azacitidine 75 mg/m2/day × 7 days in IV infusion along with 2 units of red cell concentrate prophylactically to prevent anemia. Molecular studies are needed to understand better the pathogenesis of AML with myelodysplasiarelated changes and to develop newer diagnostic and prognostic markers.","PeriodicalId":92069,"journal":{"name":"Journal of clinical and laboratory medicine","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67394702","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Use of Laboratory Decision System as a Test Utilization Management Tool in Clinical Settings, Current and Future Perspectives","authors":"L Leblow, T. Hamill, Beqaj Sh","doi":"10.16966/2572-9578.128","DOIUrl":"https://doi.org/10.16966/2572-9578.128","url":null,"abstract":"Managing laboratory test utilization has been a growing problem for the healthcare industry for a long time. With an ever-increasing number of tests, especially in the area of molecular genetics where per test costs are very high, inappropriate utilization is creating a financial burden on healthcare overall. Several large healthcare institutions have made efforts to solve this problem and have developed their own test utilization management approaches. These include, physician education, providing test pricing information, utilizing reminders in Computerized Provider Order Entry (CPOE) systems and/or setting up committees to authorize the use of expensive or complex tests. While these approaches have achieved some minor success in curbing test overutilization and generating cost savings, a robust automated Clinical Laboratory Decision Support System has still been sorely lacking. We present, here, a newly developed Laboratory Decision System, LDSTM as a potential method to address test utilization management in clinical settings. A study of provider and payer use of LDSTM revealed significant improvements in test ordering and management on both sides: a significant reduction in unnecessary tests from the provider’s side and measurable improvement in medical necessity checks from the payer’s side. methods for reducing wasteful testing. A study published by the American College of Physicians (ACP), in 2012, stemming the Tide of over treatment in U.S. Healthcare, explains “the impact of unnecessary tests on healthcare cost totals up to $250 billion a year” [4]. Timely, relevant and actionable data is needed for doctors to accurately order tests, and for labs and other rendering providers to deliver services efficiently and get paid in a timely manner. Further, the rapid growth in molecular and genetic testing (welcomed new tools for diagnosis and disease management) poses a challenge for both healthcare providers and for commercial payers regarding proper utilization of these specialized tests. Given their relatively high cost, inappropriate use of these tests represents an additional financial burden on an already over-taxed healthcare system [2,4,5]. Since laboratory testing provides 70-85% of the objective data upon which physicians base their diagnoses and treatments, laboratory diagnostics has become the single highest-volume medical activity in the U.S., with an estimated 4-5 billion tests performed annually [5]. Inappropriate testing consists of both overand underutilization, which together can dramatically increase healthcare costs. Overutilization refers to tests that are ordered when not clinically indicated, while underutilization refers to tests that are clinically indicated but not ordered. A Harvard Medical School 15Introduction Currently, physicians are challenged by a lack of access to centralized information regarding thousands of available clinical laboratory tests [1]. A study conducted by the Common wealth Fund Survey of Public ","PeriodicalId":92069,"journal":{"name":"Journal of clinical and laboratory medicine","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67394098","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Parkinson’s Disease, Lithium and Stem Cells","authors":"Burckhalter L, Gallicchio Vs","doi":"10.16966/2572-9578.126","DOIUrl":"https://doi.org/10.16966/2572-9578.126","url":null,"abstract":"Parkinson’s disease (PD) is a neurodegenerative disease characterized by loss of dopaminergic neurons in the substantia nigra. Current treatment only helps symptoms, and there is no effective treatment to delay or reverse PD. Scientists have regarded a number of genetic and environmental factors that may lead to developing PD. Lithium has been used for years in psychiatry as an effective mood stabilizer in manic depressive bipolar disorder (BD). This natural element is the most potent inhibitor of the enzyme glycogen synthetase kinase (GSK3) activity. Because of GSK3’s abundance in the body and over expression leading to PD, lithium has been postulated be used as a treatment for numerous neurodegenerative diseases and conditions where GSK3 is over expressed. Lithium has also been shown to be protective to cells against rotenone-induced PD. In contrast cellular based therapy, specifically the use of induced pluripotential stem cells (iPSCs), also pose a promising therapeutic modality for future use in order to more fully understanding the process of PD development.","PeriodicalId":92069,"journal":{"name":"Journal of clinical and laboratory medicine","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67394268","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A High Throughput Universal Vitamin D Assay for Automated Chemistry Analyzers","authors":"Saida Fb, C. Yuan","doi":"10.16966/2572-9578.132","DOIUrl":"https://doi.org/10.16966/2572-9578.132","url":null,"abstract":"A growing body of scientific literature has been showing the importance of measuring the serum levels of 25-OH Vitamin D (considered to be a reliable indicator of the overall Vitamin D status of a patient). Since the development of the first immuno-assays for 25-OH Vitamin D (over four decades ago), significant improvements have been made in the way 25-OH Vitamin D serum levels are measured. These improvements are, however, still lagging when compared to the exponential demand for Vitamin D testing in clinical settings. Indeed, current 25-OH Vitamin D assays are still slow, have low throughput and are still performed by a limited number of clinical laboratories. In this article, we report on a rapid technique to measure serum 25-OH Vitamin D that uses only two reagents and that can be adapted to virtually any automated spectrophotometric chemistry analyzer. The new technique uses polystyrene (latex) nanoparticles that have been sensitized with Vitamin D-specific antibodies. Extensive performance testing of this new 25-OH Vitamin D assay (namely the Diazyme EZ Vitamin D Assay) has been performed on one of the most common clinical chemistry analyzer (the Beckman AU680). Results show that the assay is sensitive, precise, linear, accurate and fast (throughput over 600 tests per hour). The assay has received approval for clinical use by the US Food and Drug Administration (FDA) and is certified (for accuracy and precision) by the Center for Disease Control and Prevention (CDC).","PeriodicalId":92069,"journal":{"name":"Journal of clinical and laboratory medicine","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67394685","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of Lab Request and Report Formats Completeness and its Advantage for Both Health Professionals and Patients in a Health Center, Addis Ababa, Ethiopia","authors":"T. H, Haile B, Siyum B, Mihret G, D. W.","doi":"10.16966/2572-9578.129","DOIUrl":"https://doi.org/10.16966/2572-9578.129","url":null,"abstract":"","PeriodicalId":92069,"journal":{"name":"Journal of clinical and laboratory medicine","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67394201","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}