BMC Genetics最新文献

{"title":"Genetics research at the \"Centenary of human population genetics\" conference and SBB-2019.","authors":"Tatiana V Tatarinova, Ludmila E Tabikhanova, Gilda Eslami, Haihua Bai, Yuriy L Orlov","doi":"10.1186/s12863-020-00906-7","DOIUrl":"10.1186/s12863-020-00906-7","url":null,"abstract":"","PeriodicalId":9197,"journal":{"name":"BMC Genetics","volume":"21 Suppl 1","pages":"109"},"PeriodicalIF":2.9,"publicationDate":"2020-10-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7580810/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38519087","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Disruptive natural selection by male reproductive potential prevents underexpression of protein-coding genes on the human Y chromosome as a self-domestication syndrome.","authors":"Mikhail Ponomarenko,&nbsp;Maxim Kleshchev,&nbsp;Petr Ponomarenko,&nbsp;Irina Chadaeva,&nbsp;Ekaterina Sharypova,&nbsp;Dmitry Rasskazov,&nbsp;Semyon Kolmykov,&nbsp;Irina Drachkova,&nbsp;Gennady Vasiliev,&nbsp;Natalia Gutorova,&nbsp;Elena Ignatieva,&nbsp;Ludmila Savinkova,&nbsp;Anton Bogomolov,&nbsp;Ludmila Osadchuk,&nbsp;Alexandr Osadchuk,&nbsp;Dmitry Oshchepkov","doi":"10.1186/s12863-020-00896-6","DOIUrl":"https://doi.org/10.1186/s12863-020-00896-6","url":null,"abstract":"<p><strong>Background: </strong>In population ecology, the concept of reproductive potential denotes the most vital indicator of chances to produce and sustain a healthy descendant until his/her reproductive maturity under the best conditions. This concept links quality of life and longevity of an individual with disease susceptibilities encoded by his/her genome. Female reproductive potential has been investigated deeply, widely, and comprehensively in the past, but the male one has not received an equal amount of attention. Therefore, here we focused on the human Y chromosome and found candidate single-nucleotide polymorphism (SNP) markers of male reproductive potential.</p><p><strong>Results: </strong>Examining in silico (i.e., using our earlier created Web-service SNP_TATA_Z-tester) all 1206 unannotated SNPs within 70 bp proximal promoters of all 63 Y-linked genes, we found 261 possible male-reproductive-potential SNP markers that can significantly alter the binding affinity of TATA-binding protein (TBP) for these promoters. Among them, there are candidate SNP markers of spermatogenesis disorders (e.g., rs1402972626), pediatric cancer (e.g., rs1483581212) as well as male anxiety damaging family relationships and mother's and children's health (e.g., rs187456378). First of all, we selectively verified in vitro both absolute and relative values of the analyzed TBP-promoter affinity, whose Pearson's coefficients of correlation between predicted and measured values were r = 0.84 (significance p <  0.025) and r = 0.98 (p <  0.025), respectively. Next, we found that there are twofold fewer candidate SNP markers decreasing TBP-promoter affinity relative to those increasing it, whereas in the genome-wide norm, SNP-induced damage to TBP-promoter complexes is fourfold more frequent than SNP-induced improvement (p <  0.05, binomial distribution). This means natural selection against underexpression of these genes. Meanwhile, the numbers of candidate SNP markers of an increase and decrease in male reproductive potential were indistinguishably equal to each other (p <  0.05) as if male self-domestication could have happened, with its experimentally known disruptive natural selection. Because there is still not enough scientific evidence that this could have happened, we discuss the human diseases associated with candidate SNP markers of male reproductive potential that may correspond to domestication-related disorders in pets.</p><p><strong>Conclusions: </strong>Overall, our findings seem to support a self-domestication syndrome with disruptive natural selection by male reproductive potential preventing Y-linked underexpression of a protein.</p>","PeriodicalId":9197,"journal":{"name":"BMC Genetics","volume":"21 Suppl 1","pages":"89"},"PeriodicalIF":2.9,"publicationDate":"2020-10-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1186/s12863-020-00896-6","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38519089","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Analyzing a putative enhancer of optic disc morphology.","authors":"Vladimir Babenko,&nbsp;Roman Babenko,&nbsp;Yuri Orlov","doi":"10.1186/s12863-020-00873-z","DOIUrl":"https://doi.org/10.1186/s12863-020-00873-z","url":null,"abstract":"<p><strong>Background: </strong>Genome-wide association studies have identified the CDC7-TGFBR3 intergenic region on chromosome 1 to be strongly associated with optic disc area size. The mechanism of its function remained unclear until new data on eQTL markers emerged from the Genotype-Tissue Expression project. The target region was found to contain a strong silencer of the distal (800 kb) Transcription Factor (TF) gene GFI1 (Growth Factor Independent Transcription Repressor 1) specifically in neuroendocrine cells (pituitary gland). GFI1 has also been reported to be involved in the development of sensory neurons and hematopoiesis. Therefore, GFI1, being a developmental gene, is likely to affect optic disc area size by altering the expression of the associated genes via long-range interactions.</p><p><strong>Results: </strong>Distribution of haplotypes in the putative enhancer region has been assessed using the data on four continental supergroups generated by the 1000 Genomes Project. The East Asian (EAS) populations were shown to manifest a highly homogenous unimodal haplotype distribution pattern within the region with the major haplotype occurring with the frequency of 0.9. Another European specific haplotype was observed with the frequency of 0.21. The major haplotype appears to be involved in silencing GFI1repressor gene expression, which might be the cause of increased optic disc area characteristic of the EAS populations. The enhancer/eQTL region overlaps AluJo element, which implies that this particular regulatory element is primate-specific and confined to few tissues.</p><p><strong>Conclusion: </strong>Population specific distribution of GFI1 enhancer alleles may predispose certain ethnic groups to glaucoma.</p>","PeriodicalId":9197,"journal":{"name":"BMC Genetics","volume":"21 Suppl 1","pages":"73"},"PeriodicalIF":2.9,"publicationDate":"2020-10-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1186/s12863-020-00873-z","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38525047","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genotypes diversity of env gene of Bovine leukemia virus in Western Siberia.","authors":"Natalia Blazhko,&nbsp;Sultan Vyshegurov,&nbsp;Alexander Donchenko,&nbsp;Kirill Shatokhin,&nbsp;Valeria Ryabinina,&nbsp;Kirill Plotnikov,&nbsp;Alevtina Khodakova,&nbsp;Sergey Pashkovskiy","doi":"10.1186/s12863-020-00874-y","DOIUrl":"https://doi.org/10.1186/s12863-020-00874-y","url":null,"abstract":"<p><strong>Background: </strong>This study describes the biodiversity and properties of Bovine leukemia virus in Western Siberia. This paper explores the effect of different genotypes of the env gene of the cattle leukemia virus on hematological parameters of infected animals. The researchers focused on exploring the polymorphism of the env gene and, in doing so, discovered the new genotypes I_a and I_b, which differ from genotype I. Several hypotheses on the origin of the different genotypes in Siberia are discussed.</p><p><strong>Results: </strong>We obtained varying length of the restriction fragments for genotypes I_. Additionally using restrictase Hae III were received fragments was named genotype I_a, and genotype I_b. There are 2.57 ± 0.55% (20 out of 779) samples of genotype I_b which does not differ significantly from 1% (χ² = 2.46). Other genotypes were observed in the cattle of Siberia as wild type genotypes (their frequency varied from 17.84 to 32.73%). The maximum viral load was observed in animals with the II and IV viral genotypes (1000-1400 viral particles per 1000 healthy cells), and the minimum viral load was observed animals with genotype I_b (from 700 to 900 viral particles per 1000 healthy cells).</p><p><strong>Conclusions: </strong>The probability of the direct introduction of genotype II from South America to Siberia is extremely small and it is more likely that the strain originated independently in an autonomous population with its distribution also occurring independently. A new variety of genotype I (I_b) was found, which can be both a neoplasm and a relict strain.</p>","PeriodicalId":9197,"journal":{"name":"BMC Genetics","volume":"21 Suppl 1","pages":"70"},"PeriodicalIF":2.9,"publicationDate":"2020-10-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1186/s12863-020-00874-y","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38622485","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Amyloid-β peptides slightly affect lifespan or antimicrobial peptide gene expression in Drosophila melanogaster.","authors":"Mikhail V Shaposhnikov,&nbsp;Nadezhda V Zemskaya,&nbsp;Lyubov А Koval,&nbsp;Natalya R Minnikhanova,&nbsp;Olga I Kechko,&nbsp;Vladimir A Mitkevich,&nbsp;Alexander A Makarov,&nbsp;Alexey А Moskalev","doi":"10.1186/s12863-020-00866-y","DOIUrl":"https://doi.org/10.1186/s12863-020-00866-y","url":null,"abstract":"<p><strong>Background: </strong>Beta-amyloid peptide (Aβ) is the key protein in the pathogenesis of Alzheimer's disease, the most common age-related neurodegenerative disorder in humans. Aβ peptide induced pathological phenotypes in different model organisms include neurodegeneration and lifespan decrease. However, recent experimental evidence suggests that Aβ may utilize oligomerization and fibrillization to function as an antimicrobial peptide (AMP), and protect the host from infections. We used the power of Drosophila model to study mechanisms underlying a dual role for Aβ peptides.</p><p><strong>Results: </strong>We investigated the effects of Drosophila treatment with three Aβ42 peptide isoforms, which differ in their ability to form oligomers and aggregates on the lifespan, locomotor activity and AMP genes expression. Aβ42 slightly decreased female's median lifespan (by 4.5%), but the effect was not related to the toxicity of peptide isoform. The lifespan and relative levels of AMP gene expression in male flies as well as locomotor activity in both sexes were largely unaffected by Aβ42 peptide treatment. Regardless of the effects on lifespan, Aβ42 peptide treatment induced decrease in AMP genes expression in females, but the effects were not robust.</p><p><strong>Conclusions: </strong>The results demonstrate that chronic treatment with Aβ42 peptides does not drastically affect fly aging or immunity.</p>","PeriodicalId":9197,"journal":{"name":"BMC Genetics","volume":"21 Suppl 1","pages":"65"},"PeriodicalIF":2.9,"publicationDate":"2020-10-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1186/s12863-020-00866-y","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38518655","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genetic dissection of eating and cooking qualities in different subpopulations of cultivated rice (Oryza sativa L.) through association mapping.","authors":"Chunfang Zhao,&nbsp;Ling Zhao,&nbsp;Qingyong Zhao,&nbsp;Tao Chen,&nbsp;Shu Yao,&nbsp;Zhen Zhu,&nbsp;Lihui Zhou,&nbsp;Altafhusian B Nadaf,&nbsp;Wenhua Liang,&nbsp;Kai Lu,&nbsp;Yadong Zhang,&nbsp;Cailin Wang","doi":"10.1186/s12863-020-00922-7","DOIUrl":"https://doi.org/10.1186/s12863-020-00922-7","url":null,"abstract":"<p><strong>Background: </strong>Eating and cooking qualities (ECQs) of rice (Oryza sativa L.) determine consumer acceptance and the economic value of rice varieties. The starch physicochemical properties, i.e. amylose content, gel consistency, gelatinization temperature and pasting viscosity are important indices for evaluating rice ECQs. Genetic factors are required for development of rice varieties with excellent ECQs and association mapping is one of the promising approaches for discovering such associated genetic factors.</p><p><strong>Results: </strong>A genome-wide association mapping was performed on a set of 253 non-glutinous rice accessions consisting of 83 indica and 170 japonica cultivated rice varieties through phenotyping for 11 ECQ traits in two consecutive years and genotyping with 210 polymorphic SSR and candidate-gene markers. These markers amplified 747 alleles with an average of 3.57 alleles per locus. The structure, phylogenetic relationship, and principal component analysis indicated a strong population differentiation between indica and japonica accessions and association mapping was thus undertaken within indica and japonica subpopulations. All traits showed a large phenotypic variation and highly significant phenotypic correlations were present between most of traits. A total of 33 and 30 loci were located for 11 ECQs in indica and japonica subpopulations respectively. Most of associated loci were overlapped with starch synthesis-related genes (SSRGs), and the Wx locus gathered 14 associated loci with the largest effects on amylose content, gel consistency and pasting viscosities. Eight subpopulation specific markers, RM588, Wx-(CT)_n, SSI and SBE1 for indica subpopulation and RM550, Wx^mp, SSIIa and SBE4 for japonica subpopulation, were identified, suggesting alleles of SSRGs showed the subspecific tendency. Nevertheless, allelic variation in SSIIa showed no tendency towards subspecies. One associated maker RM550 detected in japonica subpopulation for amylose content and pasting viscosity was verified a potential novel and stably expressed locus and could be selected for further fine mapping.</p><p><strong>Conclusion: </strong>This study illustrated the potential for dissecting genetic factors of complex traits in domesticated rice subspecies and provided highly associated markers to facilitate marker-assisted selection for breeding high-quality indica or japonica rice varieties.</p>","PeriodicalId":9197,"journal":{"name":"BMC Genetics","volume":" ","pages":"119"},"PeriodicalIF":2.9,"publicationDate":"2020-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1186/s12863-020-00922-7","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38486376","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"DNA methylation of CpG sites in the chicken KLF7 promoter and Exon 2 in association with mRNA expression in abdominal adipose tissue and blood metabolic indicators.","authors":"Zhiwei Zhang,&nbsp;Cunxi Nie,&nbsp;Yuechan Chen,&nbsp;Yanzhe Dong,&nbsp;Tao Lin","doi":"10.1186/s12863-020-00923-6","DOIUrl":"https://doi.org/10.1186/s12863-020-00923-6","url":null,"abstract":"<p><strong>Background: </strong>Our previous study found that chicken KLF7 was an important regulator in formation of adipose tissue. In the present study, we analyzed the association for DNA methylation in chicken KLF7 with its transcripts of abdominal adipose tissue and blood metabolic indicators.</p><p><strong>Results: </strong>The KLF7 transcripts of the adipose tissue of Chinese yellow broilers were associated with age (F = 6.67, P = 0.0035). In addition, the KLF7 transcripts were negatively correlated with blood glucose levels (r = - 0.61841, P = 0.0140). The DNA methylation levels of 26 CpG loci in the chicken KLF7 promoter and Exon 2 were studied by Sequenom MassArray. A total of 22 valid datasets were obtained. None of them was significantly different in relation to age (P > 0.05). However, the DNA methylation levels in the promoter were lower than those in Exon 2 (T = 40.74, P < 0.01). Correlation analysis showed that the DNA methylation levels of PCpG6 and E2CpG9 were significantly correlated with KLF7 transcripts and blood high-density lipoprotein levels, respectively, and many CpG loci were correlated with each other (P < 0.05). The methylation data were subjected to principal component analysis and factor analysis. The six principal components (z1-z6) were extracted and named Factors 1-6, respectively. Factor analysis showed that Factor 1 had a higher load on the loci in the promoter, and Factors 2-6 loaded highly on quite different loci in Exon 2. Correlation analysis showed that only z1 was significantly correlated to KLF7 transcripts (P < 0.05). In addition, an established regression equation between z1 and KLF7 transcripts was built, and the contribution of z1 to the variation on KLF7 transcripts was 34.29%.</p><p><strong>Conclusions: </strong>In conclusion, the KLF7 transcripts of chicken abdominal adipose tissue might be inhibited by DNA methylation in the promoter, and it might be related to the DNA methylation level of PCpG6.</p>","PeriodicalId":9197,"journal":{"name":"BMC Genetics","volume":" ","pages":"120"},"PeriodicalIF":2.9,"publicationDate":"2020-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1186/s12863-020-00923-6","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38489782","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genome wide analysis reveals genetic divergence between Goldsinny wrasse populations.","authors":"Eeva Jansson,&nbsp;Francois Besnier,&nbsp;Ketil Malde,&nbsp;Carl André,&nbsp;Geir Dahle,&nbsp;Kevin A Glover","doi":"10.1186/s12863-020-00921-8","DOIUrl":"https://doi.org/10.1186/s12863-020-00921-8","url":null,"abstract":"<p><strong>Background: </strong>Marine fish populations are often characterized by high levels of gene flow and correspondingly low genetic divergence. This presents a challenge to define management units. Goldsinny wrasse (Ctenolabrus rupestris) is a heavily exploited species due to its importance as a cleaner-fish in commercial salmonid aquaculture. However, at the present, the population genetic structure of this species is still largely unresolved. Here, full-genome sequencing was used to produce the first genomic reference for this species, to study population-genomic divergence among four geographically distinct populations, and, to identify informative SNP markers for future studies.</p><p><strong>Results: </strong>After construction of a de novo assembly, the genome was estimated to be highly polymorphic and of ~600Mbp in size. 33,235 SNPs were thereafter selected to assess genomic diversity and differentiation among four populations collected from Scandinavia, Scotland, and Spain. Global F_ST among these populations was 0.015-0.092. Approximately 4% of the investigated loci were identified as putative global outliers, and ~ 1% within Scandinavia. SNPs showing large divergence (F_ST > 0.15) were picked as candidate diagnostic markers for population assignment. One hundred seventy-three of the most diagnostic SNPs between the two Scandinavian populations were validated by genotyping 47 individuals from each end of the species' Scandinavian distribution range. Sixty-nine of these SNPs were significantly (p < 0.05) differentiated (mean F_{ST_173_loci} = 0.065, F_{ST_69_loci} = 0.140). Using these validated SNPs, individuals were assigned with high probability (≥ 94%) to their populations of origin.</p><p><strong>Conclusions: </strong>Goldsinny wrasse displays a highly polymorphic genome, and substantial population genomic structure. Diversifying selection likely affects population structuring globally and within Scandinavia. The diagnostic loci identified now provide a promising and cost-efficient tool to investigate goldsinny wrasse populations further.</p>","PeriodicalId":9197,"journal":{"name":"BMC Genetics","volume":" ","pages":"118"},"PeriodicalIF":2.9,"publicationDate":"2020-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1186/s12863-020-00921-8","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38567761","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Breeding strategies for animal resilience to weather variation in meat sheep.","authors":"Enrique Sánchez-Molano,&nbsp;Vanessa V Kapsona,&nbsp;Stavroula Oikonomou,&nbsp;Ann McLaren,&nbsp;Nicola Lambe,&nbsp;Joanne Conington,&nbsp;Georgios Banos","doi":"10.1186/s12863-020-00924-5","DOIUrl":"https://doi.org/10.1186/s12863-020-00924-5","url":null,"abstract":"<p><strong>Background: </strong>The alteration in weather patterns expected due to climate change will affect farm animal performance, probably resulting in lower quantity and quality of available products. A potential mitigation strategy would be to breed selected animals for enhanced resilience to climate change. In this context, resilience would reflect stable animal performance in spite of weather variation. The objectives of this study were to (i) derive and characterise novel animal resilience phenotypes, (ii) investigate their genetic profiles and (iii) assess the impact of integrating them in breeding strategies for genetic improvement in meat sheep.</p><p><strong>Results: </strong>Random regression models were used to jointly analyse live body weight measured in different time points throughout the growth phases of 4469 Scottish Blackface sheep and weather variables during the same period to derive novel resilience phenotypes. The genetic analysis of these phenotypes revealed significant genetic variance and heritability, and an antagonistic genetic correlation with some animal performance traits. Simulated breeding strategies demonstrated that a relative emphasis of 10% on resilience compared to other traits would enhance performance stability against weather volatility without compromising animal growth.</p><p><strong>Conclusions: </strong>Novel resilience traits exhibited sufficient genetic variation to be amenable to genetic improvement with selective breeding and are recommended to be included in future breeding goals.</p>","PeriodicalId":9197,"journal":{"name":"BMC Genetics","volume":" ","pages":"116"},"PeriodicalIF":2.9,"publicationDate":"2020-10-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1186/s12863-020-00924-5","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38467336","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Meta-analyses of genome wide association studies in lines of laying hens divergently selected for feather pecking using imputed sequence level genotypes.","authors":"Clemens Falker-Gieske, Hanna Iffland, Siegfried Preuß, Werner Bessei, Cord Drögemüller, Jörn Bennewitz, Jens Tetens","doi":"10.1186/s12863-020-00920-9","DOIUrl":"10.1186/s12863-020-00920-9","url":null,"abstract":"<p><strong>Background: </strong>Feather pecking (FP) is damaging behavior in laying hens leading to global economic losses in the layer industry and massive impairments of animal welfare. The objective of the study was to discover genetic variants and affected genes that lead to FP behavior. To achieve that we imputed low-density genotypes from two different populations of layers divergently selected for FP to sequence level by performing whole genome sequencing on founder and half-sib individuals. In order to decipher the genetic structure of FP, genome wide association studies and meta-analyses of two resource populations were carried out by focusing on the traits 'feather pecks delivered' (FPD) and the 'posterior probability of a hen to belong to the extreme feather pecking subgroup' (pEFP).</p><p><strong>Results: </strong>In this meta-analysis, we discovered numerous genes that are affected by polymorphisms significantly associated with the trait FPD. Among them SPATS2L, ZEB2, KCHN8, and MRPL13 which have been previously connected to psychiatric disorders with the latter two being responsive to nicotine treatment. Gene set enrichment analysis revealed that phosphatidylinositol signaling is affected by genes identified in the GWAS and that the Golgi apparatus as well as brain structure may be involved in the development of a FP phenotype. Further, we were able to validate a previously discovered QTL for the trait pEFP on GGA1, which contains variants affecting NIPA1, KIAA1211L, AFF3, and TSGA10.</p><p><strong>Conclusions: </strong>We provide evidence for the involvement of numerous genes in the propensity to exhibit FP behavior that could aid in the selection against this unwanted trait. Furthermore, we identified variants that are involved in phosphatidylinositol signaling, Golgi metabolism and cell structure and therefore propose changes in brain structure to be an influential factor in FP, as already described in human neuropsychiatric disorders.</p>","PeriodicalId":9197,"journal":{"name":"BMC Genetics","volume":" ","pages":"114"},"PeriodicalIF":2.9,"publicationDate":"2020-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7528462/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38447472","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}