DNA methylation of CpG sites in the chicken KLF7 promoter and Exon 2 in association with mRNA expression in abdominal adipose tissue and blood metabolic indicators.

IF 2.9 Q2 Biochemistry, Genetics and Molecular Biology

BMC Genetics Pub Date : 2020-10-14 DOI:10.1186/s12863-020-00923-6

Zhiwei Zhang, Cunxi Nie, Yuechan Chen, Yanzhe Dong, Tao Lin

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引用次数: 4

Abstract

Background: Our previous study found that chicken KLF7 was an important regulator in formation of adipose tissue. In the present study, we analyzed the association for DNA methylation in chicken KLF7 with its transcripts of abdominal adipose tissue and blood metabolic indicators.

Results: The KLF7 transcripts of the adipose tissue of Chinese yellow broilers were associated with age (F = 6.67, P = 0.0035). In addition, the KLF7 transcripts were negatively correlated with blood glucose levels (r = - 0.61841, P = 0.0140). The DNA methylation levels of 26 CpG loci in the chicken KLF7 promoter and Exon 2 were studied by Sequenom MassArray. A total of 22 valid datasets were obtained. None of them was significantly different in relation to age (P > 0.05). However, the DNA methylation levels in the promoter were lower than those in Exon 2 (T = 40.74, P < 0.01). Correlation analysis showed that the DNA methylation levels of PCpG6 and E2CpG9 were significantly correlated with KLF7 transcripts and blood high-density lipoprotein levels, respectively, and many CpG loci were correlated with each other (P < 0.05). The methylation data were subjected to principal component analysis and factor analysis. The six principal components (z1-z6) were extracted and named Factors 1-6, respectively. Factor analysis showed that Factor 1 had a higher load on the loci in the promoter, and Factors 2-6 loaded highly on quite different loci in Exon 2. Correlation analysis showed that only z1 was significantly correlated to KLF7 transcripts (P < 0.05). In addition, an established regression equation between z1 and KLF7 transcripts was built, and the contribution of z1 to the variation on KLF7 transcripts was 34.29%.

Conclusions: In conclusion, the KLF7 transcripts of chicken abdominal adipose tissue might be inhibited by DNA methylation in the promoter, and it might be related to the DNA methylation level of PCpG6.

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鸡KLF7启动子和外显子2 CpG位点的DNA甲基化与腹部脂肪组织mRNA表达和血液代谢指标的关联

背景:我们之前的研究发现，鸡KLF7是脂肪组织形成的重要调节因子。在本研究中，我们分析了鸡KLF7 DNA甲基化与其腹部脂肪组织转录本和血液代谢指标的关系。结果:中国黄肉鸡脂肪组织KLF7转录本与年龄相关(F = 6.67, P = 0.0035)。KLF7转录本与血糖水平呈负相关(r = - 0.61841, P = 0.0140)。利用Sequenom MassArray研究了鸡KLF7启动子和外显子2中26个CpG位点的DNA甲基化水平。共获得22个有效数据集。与年龄无显著差异(P > 0.05)。但该启动子DNA甲基化水平低于外显子2 DNA甲基化水平(T = 40.74, P)。结论:鸡腹部脂肪组织KLF7转录本可能受到启动子DNA甲基化的抑制，且可能与PCpG6 DNA甲基化水平有关。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

BMC Genetics 生物-遗传学

CiteScore

4.30

自引率

0.00%

发文量

审稿时长

4-8 weeks

期刊介绍： BMC Genetics is an open access, peer-reviewed journal that considers articles on all aspects of inheritance and variation in individuals and among populations.