BMC Anesthesiology最新文献

{"title":"Effect of intravenous lidocaine on Ciprofol dose in patients undergoing painless gastrointestinal endoscopy: a double-blinded, randomized, controlled trial.","authors":"Xinyu Cao, Guangli Zhu, Chongya Yang, Bin Wang, Yang Ang, Kangli Hui, Jingwei Xiong, Jiejie Zhou, Qiang Sun, Manlin Duan","doi":"10.1186/s12871-025-02934-1","DOIUrl":"10.1186/s12871-025-02934-1","url":null,"abstract":"<p><strong>Background: </strong>Ciprofol (CIP) for procedural sedation and analgesia (PSA) for painless gastrointestinal endoscopy (GE) can cause respiratory or cardiovascular complications. The literature suggests that intravenous (IV) lidocaine infusion can alleviate visceral pain and enhance humans' ventilator response to CO₂. Therefore, it was hypothesized that IV lidocaine could reduce the CIP dose for painless GE and improve recovery time.</p><p><strong>Methods: </strong>This randomized placebo-controlled trial included 40 patients undergoing GE. After CIP titration for unconsciousness, patients in group L were given IV lidocaine (1.5 mg/kg bolus dose, then a 2 mg/kg/h continuous infusion); the same volume saline as placebo was given for N group patients. The primary endpoint was the required CIP dose. Secondary endpoints were: endoscopic examination time, awakening time, post-anesthesia care unit (PACU) discharge time, pain and fatigue after awakening, adverse events, and endoscopist's and patient's satisfaction.</p><p><strong>Results: </strong>Both cohorts had comparable demographic characteristics. Group L's CIP consumption was decreased by 23.0% than the N group (47.38 ± 7.45 mg vs. 61.50 ± 9.44 mg, respectively, p < 0.001). Awakening time (P=0.002), PACU discharge time (P < 0.001), pain (P = 0.008), and fatigue (P = 0.004) after awakening were also reduced in group L. Furthermore, group L had higher satisfaction scores than group N (P = 0.017). No marked difference was identified in the incidence of unfavorable effects (P > 0.05 for all).</p><p><strong>Conclusions: </strong>Lidocaine IV infusion caused a 23.0% reduction in CIP requirements during GE. Furthermore, post-endoscopic pain and fatigue were also improved, thus suggesting that lidocaine is an efficient therapeutic option.</p><p><strong>Trial registration: </strong>This trial has been submitted to the Chinese Clinical Trial Registry (registration number: ChiCTR2300069868, registration date: 28/03/2023).</p>","PeriodicalId":9190,"journal":{"name":"BMC Anesthesiology","volume":"25 1","pages":"69"},"PeriodicalIF":2.3,"publicationDate":"2025-02-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11818029/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143405778","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Risk and benefit analysis of single-shot nerve block for postoperative analgesia for uniportal video-assisted thoracic surgery (uVATS): a randomized controlled trial.","authors":"Li Fang Wang, Fei Qi, Hong Xiang Feng, Yu Hui Shi, Yan Li, Meng Tao Zheng, Tegeleqi Bu, Wei Xia Li, Zhen Rong Zhang","doi":"10.1186/s12871-025-02955-w","DOIUrl":"10.1186/s12871-025-02955-w","url":null,"abstract":"<p><strong>Background: </strong>There is lack of the clinical evidence of optimized perioperative analgesic protocol for uniportal video-assisted thoracoscopic surgery (uVATS).</p><p><strong>Methods: </strong>We performed a RCT enrolling participants scheduled for uVATS (Trial registration: NCT06016777; registration date: Aug 28, 2023). Participants were randomized for thoracic paravertebral block combined with patient-controlled intravenous analgesia (PVB + PCIA), erector spinae block combined with PCIA (ESPB + PCIA), or PCIA group. Participants were followed-up till 6 months. Primary outcome was total opioid consumption. Secondary outcomes included postoperative rest and cough pain scores, ambulation time, chest tube duration, length of stay, anaesthesia expense and adverse events.</p><p><strong>Results: </strong>We enrolled 108 participants between October 16th, 2023 to April 14th, 2024. Neural block did not reduce opioid consumption. Postoperative rest and cough pain scores did not differ among the groups at all the follow-up time points. None of the participants experienced chronic pain. The ambulation time, duration of chest tube maintenance and length of stay did not differ among groups. Duration of anaesthesia procedure was significantly prolonged in both neural blockade groups compared to PCIA group (p = 0.033). Anaesthesia expenses were significantly higher in both nerve block groups than in the PCIA group (p < 0.001). Adverse events related to neural blockade occurred in 17.9% in PVB + PCIA group and 2.9% in ESPB + PCIA group (p = 0.010), including local haemorrhage and block failure. Adverse events related to opioid use did not differ among groups.</p><p><strong>Conclusions: </strong>Both PVB and ESPB did not exhibit analgesic advantage for uVATS. Neural block may carry the risk of haemorrhage and block failure, prolonged the anaesthesia procedure and increased the anaesthesia expenses.</p><p><strong>Trial registration: </strong>Clinical Trial Number was NCT06016777, trial registration date was Aug 28th, 2023.</p>","PeriodicalId":9190,"journal":{"name":"BMC Anesthesiology","volume":"25 1","pages":"68"},"PeriodicalIF":2.3,"publicationDate":"2025-02-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11816525/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143405798","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Efficacy of intranasal dexmedetomidine-esketamine sedation for pediatric acceptance of facemask: single-center, double-blind, randomized, controlled trial.","authors":"Kan Zhang, Guangxuan Zhang, Yanmei Zhang, Jingjing Wang, Jie Bai, Jijian Zheng, Yujuan Tao","doi":"10.1186/s12871-025-02939-w","DOIUrl":"10.1186/s12871-025-02939-w","url":null,"abstract":"<p><strong>Objective: </strong>We compare the efficacy of intranasal dexmedetomidine (DEX) and DEX-esketamine sedation on pediatric acceptance of face mask.</p><p><strong>Methods: </strong>This single-center double-blind randomized controlled study was conducted at a tertiary hospital affiliated with Shanghai Jiao Tong University. Ninety children aged 1 year to 6 years old and scheduled for elective surgery were randomly allocated in a 1:1 ratio into receiving DEX alone (n = 45) and DEX-esketamine (n = 45). DEX and esketamine were used intranasally at doses of 2 µg/kg and 2.0 mg/kg respectively. Children were assessed by an attending anesthesiologist with modified observer's assessment of alertness and sedation (MOAA/S), pediatric separation anxiety scale (PSAS) and mask acceptance scale (MAS). Perioperative adverse events (bradycardia, hypotension, hypoxia, emergence delirium etc.) were recorded.</p><p><strong>Results: </strong>Of 95 patients enrolled, 90 completed the study. The proportion of children who accepted facemask was significantly higher in the DEX-esketamine group compared to the DEX group (86.7% (39/45) vs. 62.2% (28/45), p = 0.008). Within 30 min after intranasal administration of agents, PSAS scores were similar between the two groups. Children in the DEX group were easily aroused when repositioned from the transferring bed to the operation table. In contrast, those in the DEX-esketamine group maintained a stable level of sedation (MOAA/S scores, median [25th- 75th interquartile range], 1 [1, 1] for DEX-esketamine vs. 2 [1, 4] for DEX, p < 0.001). Furthermore, subgroup analysis found that DEX-esketamine provided better facemask acceptance in children with high anxiety (PSAS ≥ 3). There were no significant differences in perioperative heart rate, noninvasive blood pressure and percutaneous arterial oxygen saturation between the two groups. Postoperative extubation time and perioperative adverse events were also comparable between the groups (all p > 0.05).</p><p><strong>Conclusions: </strong>For preoperative sedation, combination of DEX with esketamine improved mask acceptance than dexmedetomidine alone, likely due to its superior anxiolytic effect in children with high anxiety.</p><p><strong>Trial registration: </strong>This study was registered in the Chinese Clinical Trial Register (registration no. ChiCTR2400087873, registration date on 6/8/2024).</p>","PeriodicalId":9190,"journal":{"name":"BMC Anesthesiology","volume":"25 1","pages":"66"},"PeriodicalIF":2.3,"publicationDate":"2025-02-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11817246/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143398239","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of remimazolam besylate on elderly patients with mechanical ventilation: a single-center randomized controlled study.","authors":"Yihui Li, Yamin Yuan, Jinquan Zhou, Li Ma","doi":"10.1186/s12871-025-02903-8","DOIUrl":"10.1186/s12871-025-02903-8","url":null,"abstract":"<p><strong>Objective: </strong>To compare the clinical prognosis and offline strategy differences between remimazolam besylate and propofol for sedation in elderly patients undergoing mechanical ventilation.</p><p><strong>Methods: </strong>This single-center prospective randomized controlled study included elderly patients requiring invasive mechanical ventilation in the Emergency Intensive Care Unit (EICU) at The Second Hospital & Clinical Medical School of Lanzhou University from October 2021 to October 2023. Patients were randomly assigned to the remimazolam group (experimental) or propofol group (control) using respective sedation treatments. Clinical treatments remained uninfluenced. After improvement and meeting offline conditions, a cluster offline strategy guided evaluation and treatment. Data on patient demographics, vital signs, clinical outcomes, and adverse events were recorded.</p><p><strong>Results: </strong>There were no significant differences in invasive mechanical ventilation time (107.50 vs. 104.50 h, P = 0.969), ICU stay (7 days for both groups, P = 0.603), in-hospital mortality (22.5% vs. 15.0%, P = 0.39), or 28-day survival rate (69.57% vs. 69.23%, P = 0.98) between the control and experimental groups. Tracheotomy was performed in 5 control group patients and 2 experimental group patients (P = 0.235). Sedation-related delirium rates were 7.5% (control) and 5.0% (experimental) (P = 0.613).</p><p><strong>Conclusions: </strong>Remimazolam besylate and propofol showed no significant differences in safety or effectiveness for elderly patients undergoing mechanical ventilation when using the clustered offline strategy.</p>","PeriodicalId":9190,"journal":{"name":"BMC Anesthesiology","volume":"25 1","pages":"65"},"PeriodicalIF":2.3,"publicationDate":"2025-02-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11809043/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143389987","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Efficacy of continuous intravenous infusion of lidocaine on postoperative sore throat after laryngeal mask insertion: a randomized controlled trial.","authors":"Jianxin Jiang, Jimin Wu, Shuqi Shi, Xiaoli Dong, Jiaxiang Yin, Yini Wu","doi":"10.1186/s12871-025-02937-y","DOIUrl":"10.1186/s12871-025-02937-y","url":null,"abstract":"<p><strong>Background: </strong>This randomized controlled trial was performed to explore efficacy of continuous intravenous infusion of lidocaine on postoperative sore throat after laryngeal mask insertion.</p><p><strong>Methods: </strong>In this prospective trial one hundred and sixty general anesthesia surgery patients (20 to 60 years) using laryngeal mask airway were randomly divided into control group (Group C, saline as placebo), lidocaine gel group (Group LG, lidocaine gel applied to the surface of the laryngeal mask), single intravenous lidocaine group (Group SL, intravenous lidocaine 1.5 mg/kg at induction of anesthesia) and continuous infusion of lidocaine group (Group CL, a bolus of 1.5 mg/kg, followed by an infusion of 2 mg/kg/h until the end of the surgical). The primary outcomes were the incidence and severity of POST at the time of laryngeal mask removal (T1), 2 h (T2), 6 h (T3), and 24 h (T4) after removal. The secondary outcomes included the incidence of adverse events such as hoarseness, cough, and tongue numbness.</p><p><strong>Result: </strong>Within 24 h after extubation, the incidence and severity of POST was significantly lower in group CL than that in group C at all time points. In contrast, compared with group C, the incidence and severity of POST in group SL was lower only at T1. The incidence of hoarseness and cough in group CL were significantly lower than that in group C at T1 and T2. In group SL, the incidence of hoarseness and cough was lower than that in the group C only at T1. In group LG, the incidence of tongue numbness was significantly higher than that in group C only at T1, and there were no significant difference in the four groups at the other time points.</p><p><strong>Conclusion: </strong>Continuous infusion of lidocaine is effective in reducing the incidence and severity of POST after laryngeal mask ventilation, as well as reducing the incidence of adverse effects such as hoarseness and cough.</p><p><strong>Trial registration: </strong>Chinese Clinical Trial Registry (ChiCTR2300070339,04/10/2023).</p>","PeriodicalId":9190,"journal":{"name":"BMC Anesthesiology","volume":"25 1","pages":"63"},"PeriodicalIF":2.3,"publicationDate":"2025-02-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11806593/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143373812","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Predicting postoperative nausea and vomiting after cesarean section: a nomogram model combined with gastric ultrasound.","authors":"Yingchao Liu, Huohu Zhong, Zhisen Dai, Yuxin Huang, Yibin Liu, Hefan He, Yuewen Liao, Weifeng Liu","doi":"10.1186/s12871-025-02936-z","DOIUrl":"10.1186/s12871-025-02936-z","url":null,"abstract":"<p><strong>Background: </strong>To investigate the independent risk factors associated with postoperative nausea and vomiting (PONV) following Cesarean section procedures, and establish and validate a nomogram to predict them.</p><p><strong>Methods: </strong>The clinical data of 116 adult patients who underwent Cesarean section procedures between August 2022 and February 2023 were included. Participants were randomly divided into training (n = 87) and verification sets (n = 29) in a 3:1 ratio. Univariate and multivariate logistic regression were used to analyze the risk factors for PONV following Cesarean sections and the independent risk factors were then used for the prediction model. Simultaneously, 29 adult patients who underwent caesarean section between February 2023 and April 2023 were included in the hospital as a test set to conduct external verification of the nomogram and Apfel scoring models, and compare their diagnostic efficacy in predicting PONV after caesarean section.</p><p><strong>Results: </strong>A history of motion sickness, systolic blood pressure reduction > 20%, and gastric volume were independent risk factors for PONV and used to construct the model. The AUC for predicting the risk of PONV in the training and validation sets was 0.814 (95% confidence interval [CI] = 0.709-0.918) and 0.792 (95% CI = 0.621-0.962), respectively. In the test set, the AUCs of the nomogram and the Apfel scoring models were 0.779 (95% CI = 0.593-0.965) and 0.547 (95% CI = 0.350-0.745), respectively, with the former being significantly higher (Z = 2.165, P < 0.05).</p><p><strong>Conclusions: </strong>Our nomogram model was superior to the Apfel scoring model and may be helpful in formulating appropriate individualized management strategies for nausea and vomiting following Cesarean sections, to promote the rapid recovery of patients.</p>","PeriodicalId":9190,"journal":{"name":"BMC Anesthesiology","volume":"25 1","pages":"64"},"PeriodicalIF":2.3,"publicationDate":"2025-02-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11806823/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143373820","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of balanced opioid-free anesthesia on post-operative nausea and vomiting in patients undergoing video-assisted thoracic surgery: a randomized trial.","authors":"Xiang Yan, Chen Liang, Jia Jiang, Ying Ji, An-Shi Wu, Chang-Wei Wei","doi":"10.1186/s12871-025-02938-x","DOIUrl":"10.1186/s12871-025-02938-x","url":null,"abstract":"<p><strong>Objectives: </strong>Postoperative nausea and vomiting (PONV) is common after video-assisted thoracic surgery, which may be associated with the use of intraoperative opioids. We tested the hypothesis that balanced opioid-free anesthesia (OFA) might reduce the incidence of PONV after video-assisted thoracic surgery.</p><p><strong>Methods: </strong>One hundred and sixty-eight adults undergoing video-assisted thoracic assisted surgery were randomly assigned to receive balanced opioid-free anesthesia or balanced opioid-based anesthesia (OBA). The primary outcome was the incidence of PONV, which was assessed with the Myles's simplified PONV impact scale during the initial 24 h after surgery.</p><p><strong>Results: </strong>Compared with OBA group, the overall incidence of PONV in OFA group was significant reduced (14.6% vs. 30.1%, P = 0.017), and OFA reduced the risk of PONV events within 24 h of surgery (HR, 0.44; 95%CI: 0.22-0.87, P = 0.018). The incidence of other postoperative complications in OFA group was lower than that in OBA group (19.5% vs. 33.7%, P = 0.039). The quality of recovery, distance of 6-minute walk test, pain scores, and 36-item short form survey were comparable at each time points.</p><p><strong>Conclusion: </strong>In patients undergoing video-assisted thoracic surgery, the use of balanced OFA anesthesia can help reduce the incidence of PONV events. This anesthetic regimen has shown good feasibility without significantly increasing the patient's pain score and complications.</p><p><strong>Clinical trial registration number: </strong>Clinicaltrials.gov, Identifier: NCT05411159. First posted date: 9 Jun, 2022.</p>","PeriodicalId":9190,"journal":{"name":"BMC Anesthesiology","volume":"25 1","pages":"62"},"PeriodicalIF":2.3,"publicationDate":"2025-02-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11806895/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143373804","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of a positive end-expiratory pressure on oxygenation, respiratory compliance, and hemodynamics in obese patients undergoing laparoscopic surgery in reverse Trendelenburg position: a systematic review and meta-analysis of randomized controlled trials.","authors":"Gulfairus A Yessenbayeva, Aizhan M Meyerbekova, Sergey I Kim, Murat B Zhumabayev, Gulbanu S Berdiyarova, Sanzhar B Shalekenov, Dinara S Zharlyganova, Irina Y Mukatova, Yekaterina A Yukhnevich, Dmitriy A Klyuyev, Andrey I Yaroshetskiy","doi":"10.1186/s12871-025-02933-2","DOIUrl":"10.1186/s12871-025-02933-2","url":null,"abstract":"<p><strong>Background: </strong>High and individual positive end-expiratory pressure (PEEP) during laparoscopic surgery may improve oxygenation and respiratory mechanics.</p><p><strong>Methods: </strong>We searched RCTs in PubMed, Cochrane Library, Web of Science, and Google Scholar from from from January 2000 to December 2023 comparing the different intraoperative PEEP (low PEEP (LPEEP): 0-5 mbar; moderate PEEP (MPEEP): 6-9 mbar; high PEEP (HPEEP): >=10 mbar; individualized PEEP (iPEEP): PEEP set by special physiological technique) on arterial oxygenation, respiratory compliance (Cdyn) or driving pressure, mean arterial pressure (MAP), and heart rate (HR) in patients during laparoscopic surgery in reverse Trendelenburg position. We calculated mean differences (MD) with 95% confidence intervals (CI), and predictive intervals (PI) using random-effects models. The Cochrane Bias Risk Assessment Tool was applied.</p><p><strong>Results: </strong>8 RCTs (n = 425) met the inclusion criteria. HPEEP vs. LPEEP increased PaO₂/FiO₂ (+ 129.93 [+ 75.20; +184.65] mmHg, p < 0.0001) with high variation of true effect (Chi² 34.92, p < 0.0001; I² 89%). iPEEP vs. LPEEP also increased PaO₂/FiO₂ + 130.23 [+ 57.18; +203.27] mmHg, p = 0.0005) with high variation of true effect (Chi² 26.95, p < 0.0001; I² 93%). HPEEP vs. LPEEP increased Cdyn (+ 15.06 [5.47; +24.65] ml/mbar, p = 0.002) with high variation of true effect (Chi² 93.16, p < 0.0001; I² 96%). iPEEP vs. LPEEP increased Cdyn (+ 22.46 [+ 8.56; +36.35] ml/mbar, p = 0.002) with high variability of the true effect (Chi² 53.92, p < 0.0001; I² 96%). HPEEP group had higher MAP as compared to LPEEP) + 4.36 [+ 0.36;+8.36], p = 0.03), variability of the true effect was nonsignificant. HR did nit differ between all comparisons.</p><p><strong>Conclusion: </strong>In patients with obesity undergoing surgery in the reverse Trendelenburg position HPEEP and iPEEP may improve oxygenation, decrease driving pressure, and increase dynamic compliance compared to LPEEP with high variation of true effect without relevant hemodynamic compromise. Data with MPEEP comparisons are inconclusive.</p><p><strong>Prospero registration: </strong>CRD42023488971; registered December 14, 2023.</p>","PeriodicalId":9190,"journal":{"name":"BMC Anesthesiology","volume":"25 1","pages":"61"},"PeriodicalIF":2.3,"publicationDate":"2025-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11803948/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143363659","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impaired hippocampal neurogenesis associated with regulatory ceRNA network in a mouse model of postoperative cognitive dysfunction.","authors":"Jingrun Lin, Xiaoqiu Zhu, Xuan Li, Yu Hong, Yaohui Liang, Siqi Chen, Chenzhuo Feng, Lin Cao","doi":"10.1186/s12871-025-02928-z","DOIUrl":"10.1186/s12871-025-02928-z","url":null,"abstract":"<p><strong>Background: </strong>Postoperative cognitive dysfunction (POCD) represents a post-surgical complication that features progressive cognitive impairment and memory loss, often occurring in elderly patients. This study aimed to investigate the potential biological mechanisms underlying POCD.</p><p><strong>Methods: </strong>Male C57BL/6 mice (2 and 17 months old) were randomly assigned to surgery or control groups. The surgery group underwent laparotomy under 1.5% isoflurane anesthesia, while controls received no intervention. Cognitive function was assessed 7-10 days post-surgery using open field, Y-maze, and novel object recognition tests. Hippocampal mRNA expression was analyzed using Encyclopedia of Genes and Genomes (KEGG) and Gene Ontology (GO) enrichment. A competing endogenous RNA (ceRNA) network was constructed using microRNA (miRNA) target prediction databases (miRanda, miRTarbase, miRcode) and sequencing results. Key findings were validated by RT-qPCR and immunofluorescence. The Connectivity Map (CMap) database was queried to predict potential POCD treatments.</p><p><strong>Results: </strong>Aging significantly affected mice's spontaneous activity in the open field test (F1, 28 = 8.933, P < 0.01) and the proportion of time spent in the center area (F1, 28 = 5.387, P < 0.05). Surgery significantly reduced the rate of spontaneous alternations in the Y-maze (F1, 28 = 16.94, P < 0.001) and the recognition index in novel object recognition test (F1, 28 = 6.839, P < 0.05) in aging mice, but had no effect on young mice. Transcriptome analysis revealed that aging and surgery downregulated multiple neurogenesis-related genes in the hippocampus. Doublecortin (DCX) immunofluorescence staining confirmed reduced hippocampal neurogenesis in aging mice, which was further decreased after surgery. We identified several key lncRNAs and miRNAs implicated in neurogenesis regulation. Additionally, drugs were predicted as potential therapeutic candidates for POCD treatment.</p><p><strong>Conclusion: </strong>Both aging and surgery have complex effects on the hippocampal transcriptome in mice. The significant decrease in neurogenesis may be a potential reason for the increased susceptibility of aging mice to POCD. The identified key regulatory lncRNAs, miRNAs, and drugs provide potential therapeutic targets for POCD prevention and treatment.</p>","PeriodicalId":9190,"journal":{"name":"BMC Anesthesiology","volume":"25 1","pages":"60"},"PeriodicalIF":2.3,"publicationDate":"2025-02-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11800588/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143363784","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Application of a novel extracorporeal membrane oxygenation system in awake Hu sheep under various durations.","authors":"Shuo Ding, Juan Chen, Yanchao Wu, Huiru Lin, Qiqiang Liang, Gaoqin Teng, Zhenjie Liu, Man Huang","doi":"10.1186/s12871-025-02930-5","DOIUrl":"10.1186/s12871-025-02930-5","url":null,"abstract":"<p><strong>Background: </strong>Extracorporeal membrane oxygenation (ECMO) provides effective support for respiratory and circulatory functions and stands as an essential means in the management of life-threatening conditions. This study aimed to evaluate the safety and efficacy of a novel ECMO system in awake sheep models across various support durations.</p><p><strong>Methods: </strong>Ten healthy Hu sheep were divided into venovenous (VV) and venoarterial (VA) groups, further categorized into short, medium, and long-term observation subgroups. Vital signs and blood indices were meticulously monitored. The integrity of heparin coatings and thrombosis in the ECMO system were assessed at indicated time points, followed by histopathological analysis.</p><p><strong>Results: </strong>All ten sheep survived to their planned endpoints under awake ECMO, exhibiting stable vital signs without notable reductions in blood cells. With the support of ECMO, all sheep demonstrated a significant increase in partial pressure of oxygen (PO₂) and maintained oxygen saturation (SO₂) between 99.8 and 100%. The heparin coating remained relatively intact even after prolonged ECMO operation. Anatomical observations and pathological histology analyses of major organs revealed no apparent abnormalities.</p><p><strong>Conclusions: </strong>In healthy Hu sheep models with different maintenance periods, the novel ECMO support in awake state demonstrated high safety and feasibility.</p>","PeriodicalId":9190,"journal":{"name":"BMC Anesthesiology","volume":"25 1","pages":"59"},"PeriodicalIF":2.3,"publicationDate":"2025-02-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11800640/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143363747","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}