Bioseparation最新文献_第4页

The potential of polymeric cryogels in bioseparation. 高分子低温材料在生物分离中的应用前景。

Bioseparation Pub Date : 2001-01-01 DOI: 10.1023/a:1016386902611

V I Lozinsky, F M Plieva, I Y Galaev, B Mattiasson

{"title":"The potential of polymeric cryogels in bioseparation.","authors":"V I Lozinsky, F M Plieva, I Y Galaev, B Mattiasson","doi":"10.1023/a:1016386902611","DOIUrl":"https://doi.org/10.1023/a:1016386902611","url":null,"abstract":"This is a review discussing the production and properties of cryogels (from the Greek kappa rho iota sigma (kryos) meaning frost or ice), immobilization of ligands in cryogels and the application of affinity cryogels in bioseparation. Cryotropic gel formation proceeds in a non-frozen liquid microphase existing in the macroscopically frozen sample. Due to the cryoconcentration of gel precursors in the non-frozen liquid microphase, cryogelation is characterised by a decrease in the critical concentration of gelation and an increase in gelation rates compared with traditional gelation at temperatures above freezing point. Cryogels can be obtained through the formation of both physically and covalently cross-linked heterogeneous polymer networks. Interconnected systems of macropores and sponge-like morphology are typical for cryogels, allowing unhindered diffusion of solutes of practically any size. Most of the water present in spongy cryogels is capillary bound and can be removed mechanically by squeezing. The properties of cryogels can be regulated by the temperature of cryogelation, the time the sample is kept in a frozen state and freezing/thawing rates, by the nature of the solvent and by the use of soluble and insoluble additives. The unique macroporous morphology of cryogels, in combination with osmotic, chemical and mechanical stability, makes them attractive matrices for chromatography of large entities such as protein aggregates, membrane fragments, viruses, cell organells and even whole cells. Special attention is given to immunosorption of viruses on cryogel-based sorbents. As chromatographic materials, cryogels can be used both in bead form and as spongy cylindrical blocks (monoliths) synthesized inside the chromatographic column. The macroporous nature of cryogels is also advantageous for their application as matrices in the immobilization of biocatalysts operating in both aqueous and organic solvents. New potential applications of cryogels are discussed.","PeriodicalId":9179,"journal":{"name":"Bioseparation","volume":"10 4-5","pages":"163-88"},"PeriodicalIF":0.0,"publicationDate":"2001-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1023/a:1016386902611","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21992531","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 321

Electrophoretic methods for fractionation of native and heat-denatured bovine beta-lactoglobulin. 分离天然和热变性牛β -乳球蛋白的电泳方法。

Bioseparation Pub Date : 2001-01-01 DOI: 10.1023/a:1016336322713

K H Valkonen, N Marttinen, T Alatossava

引用次数: 8

A study of the spatial organisation of microbial cells in a gel matrix subjected to treatment with ultrasound standing waves. 用超声驻波处理凝胶基质中微生物细胞的空间组织的研究。

Bioseparation Pub Date : 2001-01-01 DOI: 10.1023/a:1016311410219

L Gherardini, S Radel, S Sielemann, O Doblhoff-Dier, M Gröschl, E Benes, A J McLoughlin

{"title":"A study of the spatial organisation of microbial cells in a gel matrix subjected to treatment with ultrasound standing waves.","authors":"L Gherardini, S Radel, S Sielemann, O Doblhoff-Dier, M Gröschl, E Benes, A J McLoughlin","doi":"10.1023/a:1016311410219","DOIUrl":"https://doi.org/10.1023/a:1016311410219","url":null,"abstract":"Retention and manipulation of microbial cells through exploitation of ultrasonic forces has been reported as a novel cell immobilisation technique. The spatial ordering of yeast cells, within suspensions subjected to an ultrasonic standing wave field, was analysed for the first time. A technique, based on 'freezing' the spatial arrangement using polymer gelation was developed. The resultant gel was then sectioned and examined using microscopic techniques. Light Microscopy confirmed the presence of specific regions in the ultrasonic field, where the cells are organised into bands corresponding to the standing waves' pressure nodal planes. Computer Image Analysis measurement of several physical parameters associated with this cell distribution matched the values derived from the theoretical model. The spatial cell-cell re-arrangement within each band and uneven distribution along the nodal planes have been analysed by Scanning Electron Microscopy. These results complement the ongoing study of the process of immobilisation of microbial cells by ultrasound standing waves.","PeriodicalId":9179,"journal":{"name":"Bioseparation","volume":"10 4-5","pages":"153-62"},"PeriodicalIF":0.0,"publicationDate":"2001-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1023/a:1016311410219","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21992530","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 27

Physical properties of detergent-based aqueous two-phase systems. 以洗涤剂为基础的水两相体系的物理性质。

Bioseparation Pub Date : 2001-01-01 DOI: 10.1023/a:1016331228174

K Selber, S Müller, H Gieren, J Thömmes, T Sottmann, R Strey, M R Kula

{"title":"Physical properties of detergent-based aqueous two-phase systems.","authors":"K Selber, S Müller, H Gieren, J Thömmes, T Sottmann, R Strey, M R Kula","doi":"10.1023/a:1016331228174","DOIUrl":"https://doi.org/10.1023/a:1016331228174","url":null,"abstract":"The physical behavior of the binary phase systems of the non-ionic polyoxyethylene detergent Agrimul NRE 1205 and water was investigated. This technical detergent can be used for the large-scale recovery of biomolecules in detergent based aqueous two-phase systems. The phase diagram was determined. It shows significant and unexpected differences to highly purified detergents. Very similar to neat detergents the phase diagram can be influenced by auxiliary chemicals thus shifting the entire phase diagram in general to lower temperatures. This was demonstrated by lowering the cloud-point by various additions. The concentration factor, as an important parameter of a first capture step in purification was investigated and modeled. Auxiliary chemicals, temperature change and change in detergent concentration also influence the viscosity and density of the phases. These experimental data are shown. They can help to explain the separation behavior of proteins. In large-scale separations aqueous two-phase systems are separated using disc-stack centrifuges. It is demonstrated that this is not a feasible method for detergent-based aqueous two-phase extraction and the physical reason is presented.","PeriodicalId":9179,"journal":{"name":"Bioseparation","volume":"10 4-5","pages":"243-53"},"PeriodicalIF":0.0,"publicationDate":"2001-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1023/a:1016331228174","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21993122","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 9

Buoyancy-induced mixing during wash and elution steps in expanded bed adsorption 膨胀床吸附中洗涤和洗脱步骤中浮力诱导的混合

Bioseparation Pub Date : 2001-01-01 DOI: 10.1023/A:1012044024909

C. Fee, A. Liten