Bioscience Biotechnology Research Communications最新文献_第9页

Enhancing the Occupational Health Safety among Radiology Nurses Working in the Hospital of Gurugram, Haryana, India 加强印度哈里亚纳邦古鲁格拉姆医院放射科护士的职业健康安全

Bioscience Biotechnology Research Communications Pub Date : 2022-03-25 DOI: 10.21786/bbrc/15.1.27

R. Deep

引用次数: 1

High Performance Thin-Layer Chromatography-Mass Spectrometry Evaluation of Sanguinarine and Dihydrosanguinarine from Argemone mexicana Seeds in Edible Mustard Oil 高效薄层色谱-质谱法评价食用芥菜油中墨西哥银皂籽中血根碱和二氢血根碱的含量

Bioscience Biotechnology Research Communications Pub Date : 2022-03-25 DOI: 10.21786/bbrc/15.1.24

Ramakant Yadav

{"title":"High Performance Thin-Layer Chromatography-Mass Spectrometry Evaluation of Sanguinarine and Dihydrosanguinarine from Argemone mexicana Seeds in Edible Mustard Oil","authors":"Ramakant Yadav","doi":"10.21786/bbrc/15.1.24","DOIUrl":"https://doi.org/10.21786/bbrc/15.1.24","url":null,"abstract":"In this present study, a simple, rapid, cheap, sensitive and reproducible HPTLC-MS Method has been developed for the identification of two important bioactive compounds, Sanguinarine and Dihydrosanguinarine in Argemone mexicana Linn seeds. The work further discussed and developed a sensitive HPTLC – MS method to analyse the adulteration and/or contamination of argemone oil in the edible mustard oil by spiking Sanguinarine and Dihydrosanguinarine as biomarkers. The n-hexane: diethyl ether (1:1 v/v) solvent system has been used as an extraction medium to extract the Sanguinarine and Dihydrosanguinarine from the seeds followed by HPLC-MS detection.The CAMAG HPTLC system modules consisted with ATS-4, ADC-2, visualizer-2, TLC scanner-4; Derivatizer and TLC-MS interface-2 have been used for sample application, HPTLC plate development, plate photo documentation, scanning of plate, spraying of derivatization reagent and elution of biomarkers directly from HPTLC plate respectively. The n-hexane: acetone (23:7, v/v) and dragendorff’s reagent has been used as mobile phase and derivatization reagent respectively. The UV- spectra and MS data confirmed the detection of selected biomarkers in the samples/spiked samples. Thus, the work highlights the use of HPTLC-MS to develop simple and routine method for the sensitive detection of Sanguinarine and Dihydrosanguinarine in Argemone mexicana seeds and able to become bases to these biomarkers’ evaluations in other samples such as various edible oils, agro-products, drugs and biomedical products etc.","PeriodicalId":9156,"journal":{"name":"Bioscience Biotechnology Research Communications","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45128044","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Emergency Department Revisit Rate of Chronic Obstructive Pulmonary Disease Patients On Oral Corticosteroids: An Observational Study 慢性阻塞性肺疾病患者口服皮质类固醇的急诊重访率:一项观察性研究

Bioscience Biotechnology Research Communications Pub Date : 2022-03-25 DOI: 10.21786/bbrc/15.1.6

T. Ismaeil

引用次数: 0

Morin mitigates Chronic and Unpredictable Mild Stress-Induced Depression by Regulation of Endoplasmic Reticulum Stress and Brain-Derived Neurotrophic Factor-Mediated Apoptosis Morin通过调节内质网应激和脑源性神经营养因子介导的细胞凋亡减轻慢性和不可预测的轻度应激诱导的抑郁症

Bioscience Biotechnology Research Communications Pub Date : 2022-03-25 DOI: 10.21786/bbrc/15.1.29

A. Thenmozhi

{"title":"Morin mitigates Chronic and Unpredictable Mild Stress-Induced Depression by Regulation of Endoplasmic Reticulum Stress and Brain-Derived Neurotrophic Factor-Mediated Apoptosis","authors":"A. Thenmozhi","doi":"10.21786/bbrc/15.1.29","DOIUrl":"https://doi.org/10.21786/bbrc/15.1.29","url":null,"abstract":"According to the report of World Health Organization, it is expected that depression will be one of the major causes for disability and disease in the world, by 2030. Currently used drugs will not be effective to the depressive patients, due to their lower efficiency with side effects. Hence, there is a need to concentrate on natural products to overcome therapeutic hassles. Morin, a bioflavonoid found in fruits, vegetables, some herbs and wine is reported to have an antidepressant-like effect against acute stress conditions. It is reported to possess various pharmacological properties such as antioxidant, anti-diabetic, hepatoprotective, anti-cancer and anti-inflammatory activities. To understand the antidepressant effect of morin against unpredicted chronic mild stress (UCMS), rats were divided into normal, UCMS alone, UCMS and morin (30 & 60 mg/kg) and morin alone (60 mg/kg) groups. Serum corticosterone levels, expression of brain derived neurotrophic factor (BDNF), apoptotic and endoplasmic reticulum (ER) stress related indices were compared among the groups. UCMS alone exposed rats showed less crossings with diminished activities in open field test (OFT), increased serum corticosterone levels and enhanced expression of BDNF signaling and ER stress related markers associated with apoptosis as compared to control. In contrast, morin (60 mg/kg b.w.) cotreatment attenuated UCMS induced abnormalities as compared to UCMS alone exposed animals. The present results indicated the antidepressant-like actions of morin against UCMS in rats were partially due to its anti-apoptotic effects by regulating BDNF/Trk-B and ER stress related markers. Moreover the results of the present study indicated that the morin may act as therapeutic agent for the management of depression alone or with other currently used antidepressants.","PeriodicalId":9156,"journal":{"name":"Bioscience Biotechnology Research Communications","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44409999","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

On the Diversity of Beetles in the Baghdad Campus, Islamia University of Bahawalpur, Pakistan 巴基斯坦巴哈瓦尔布尔伊斯兰大学巴格达校区甲虫多样性研究

Bioscience Biotechnology Research Communications Pub Date : 2022-03-25 DOI: 10.21786/bbrc/15.1.21

K. Nadeem

{"title":"On the Diversity of Beetles in the Baghdad Campus, Islamia University of Bahawalpur, Pakistan","authors":"K. Nadeem","doi":"10.21786/bbrc/15.1.21","DOIUrl":"https://doi.org/10.21786/bbrc/15.1.21","url":null,"abstract":"Coleoptera fauna in Baghdad Campus was investigated from July 2020 to April 2021. Three habitats included inter dunal sandy area, core desert area, and area in and around the hostel which have been named as H-1, H-2 and H-3 respectively. Techniques like hand picking, plant jerking and pitfall trapping were adopted for sampling in order to explore distribution and diversity of Coleoptera fauna. 1037 samples were collected and preserved in 70% alcohol. Samples were identified on the basis of morphological characters by using bug guide. 7 families and 16 genera were sorted out. The family Tenebrionidae include the genera Blaps, Eleodes, Pimelia, Gonocephallu, and Eusattus was present. The family Coccinellidae includes genera Coccinella was also found. The family Carabidae includes Anthia, Scarites and Calosoma. The family Elateridae, Carculionidae, and Chrysomelidae contains genera Agriotes, Rhynchophorus, and Altica respectively. The Family Scarabaeidae contains the Thyce, Heteronychus, Tomarus, Phyllophagaa genera. In total count, maximum diversity and abundance of Altica was reported. Genus Anthia, Scarites, Agriotes, Rhynnchophorus, Heteronychus, and Tomarus were found in minimum but equal number. In habitat-3, maximum diversity was observed as compared to other habitats. It is concluded that these findings seemed to be helpful in ecological management of the beetles","PeriodicalId":9156,"journal":{"name":"Bioscience Biotechnology Research Communications","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46324399","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effectiveness of Video-Assisted Teaching on Knowledge and Attitude Regarding Attention Deficit Hyperactivity Disorder among Primary School Teachers in Gurugram, Haryana, India: A Pre-Experimental Study 印度哈里亚纳邦古鲁格拉姆小学教师对注意缺陷多动障碍知识和态度的视频辅助教学效果：实验前研究

Bioscience Biotechnology Research Communications Pub Date : 2022-03-25 DOI: 10.21786/bbrc/15.1.23

R. Deep

{"title":"Effectiveness of Video-Assisted Teaching on Knowledge and Attitude Regarding Attention Deficit Hyperactivity Disorder among Primary School Teachers in Gurugram, Haryana, India: A Pre-Experimental Study","authors":"R. Deep","doi":"10.21786/bbrc/15.1.23","DOIUrl":"https://doi.org/10.21786/bbrc/15.1.23","url":null,"abstract":"The study was conducted in selected schools of Gurugram, Haryana. Sixty (60) primary school teachers were selected from three schools of Gurugram by using total enumeration sampling technique. Self-structured knowledge questionnaire and attitude rating scale was used to assess the knowledge and attitude of primary school teachers regarding ADHD. The findings revealed that in the pre-test, majority 41(68.3%) of teachers had inadequate knowledge and 19(31.7%) had moderate knowledge and 28(46.7%) had unfavourable attitude and 32(53.3%) had Neutral attitude whereas in the post-test, 15(25%) had moderate knowledge and majority 45(75%) had adequate knowledge and 13(21.7%) had neutral attitude and majority 47(78.3%) had favourable attitude regarding ADHD. Paired “t” test was used to observe differences between pre and post-test mean scores and were found statistically significant at 0.05 level. The study concluded that educational material like video assisted teaching had shown significant improvement in knowledge and attitude of primary school teachers.","PeriodicalId":9156,"journal":{"name":"Bioscience Biotechnology Research Communications","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47123237","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Population Status, Distribution, Threats and Conservation of the Blackbuck, Antilope cervicapra in South Asia: An Updated Review 南亚黑腹鹬种群现状、分布、威胁和保护：最新综述

Bioscience Biotechnology Research Communications Pub Date : 2022-03-25 DOI: 10.21786/bbrc/15.1.4

Rabia Tahir

{"title":"Population Status, Distribution, Threats and Conservation of the Blackbuck, Antilope cervicapra in South Asia: An Updated Review","authors":"Rabia Tahir","doi":"10.21786/bbrc/15.1.4","DOIUrl":"https://doi.org/10.21786/bbrc/15.1.4","url":null,"abstract":"Blackbuck, Antilope cervicapra, is a diurnal ungulate species with distinct sexual dimorphism and spellbinding beauty. Male blackbuck has mesmeric beauty with its unique darker coat, showing the increased intensity of color with age. Blackbuck is endemic to Pakistan, Nepal, India and Bangladesh but now its population is reduced to a few areas. Threats such as hunting, stress, habitat loss, diseases, poaching, road accidents, habitat fragmentation, interspecific competition, predation pressure etc., have reduced the population size of blackbuck to a threatened level. Therefore, different conservation strategies are underway to increment its count for improvement of faunal diversity, tourism development and dispersal of the local culture in South Asia. Captive breeding of species is the most efficacious conservation strategy in South Asia so far. Furthermore, various rules and regulations along with strategies like hormone-mediated conservation by injections of prostaglandin and artificial insemination are assisting the species by increasing its birth rate. Genetic studies, introduction to non-endemic but suitable habitat and religious affiliation of communities also contributed to blackbuck conservation. Current conservation practices are helping to conserve the blackbuck but are associated with a few concerns also, thereby proper management, planning, monitoring of conservation practices is required. Population size, distribution range, threats confronted by species, conservation practi","PeriodicalId":9156,"journal":{"name":"Bioscience Biotechnology Research Communications","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44102134","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effectiveness of Osteopathic Treatment Versus Modified Cervical Exercise Therapy in Patients with Text Neck Syndrome 整骨疗法与改良颈椎运动疗法对短信颈综合征患者的疗效比较

Bioscience Biotechnology Research Communications Pub Date : 2022-03-25 DOI: 10.21786/bbrc/15.1.41

M. Miraj

{"title":"Effectiveness of Osteopathic Treatment Versus Modified Cervical Exercise Therapy in Patients with Text Neck Syndrome","authors":"M. Miraj","doi":"10.21786/bbrc/15.1.41","DOIUrl":"https://doi.org/10.21786/bbrc/15.1.41","url":null,"abstract":"Text neck syndrome is a stress/overuse injury in the neck caused by leaning forward for extended periods of time, such as when reading and texting on a cellphone, and has been linked to stress injuries. The primary cause of the disorder is the weight of the head which while bending it forward or looking down at a 45 to 60 degree angle during texting produces around 50 to 60 pounds of stress on the neck, increasing pressure over time and causing damage. While the majority of treatment is mostly based on. The aim of the present study was to evaluate the effectiveness of treatment between osteopathic intervention and modified cervical exercise therapy program among text neck syndrome patients. In total, seventy two patients were selected based on inclusion/exclusion criteria out of which 6 dropped out. In the end, 66 patients were randomly were allocated into two groups namely, the osteopathic treatment group(n=33) and modified cervical exercise group(n=33). The treatment program was followed for 3 weeks and the patients were evaluated for Numeric Pain Rating Scale(NPRS), Neck disability index(NDI) , Cervical range of motion as well as cranio-vertebral angle to measure the treatment effectiveness. Paired t-test showed significant differences in both groups between variables mean scores from the baseline readings after three weeks of intervention. Statistical significant differences were observed in the mean scores for all the outcome measures including NPRS, Cervical ROM, Craniovertebral angle and NDI. The result of the study suggests that Osteopathic treatment technique along with modified cervical exercise therapy is more effective than modified cervical exercise alone in treating text neck syndrome patients(p<0.05).","PeriodicalId":9156,"journal":{"name":"Bioscience Biotechnology Research Communications","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43487455","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Effects of Costus speciosus Root Extract on Cultured Human Lung Cancer Cells, A549 木香根提取物对培养人肺癌细胞的影响

Bioscience Biotechnology Research Communications Pub Date : 2022-03-25 DOI: 10.21786/bbrc/15.1.25

A. Omar

{"title":"The Effects of Costus speciosus Root Extract on Cultured Human Lung Cancer Cells, A549","authors":"A. Omar","doi":"10.21786/bbrc/15.1.25","DOIUrl":"https://doi.org/10.21786/bbrc/15.1.25","url":null,"abstract":"Cancer has become one of the most common clinical reasons of death all over the world, with more than nine million deaths each year. Throughout the history, many traditional plants have been used to treat various types of cancers. The scientifically researched anticancer plants, with over 3000 species are novel candidates for anticancer drugs, because more than 80% of society believes in traditional medicine as its cure. The aim of this study was to determine the effects of Costus speciosus root extract on human lung cancer (LC) cells (A549). Treatment of (A549) cells with ethyl acetate Costus root extract significantly lowered the cell viability. IC50 was calculated, after 24, 48 and72 h, with concentration of 25, 40, 70 and 100 μg/100μl. The MTT assay was used to test the viability of the cells. The effect of (EACR) on cell adhesion and migration was demonstrated in a wound healing assay, mitochondrial membrane potential was detected in a JC-1 assay, and DNA damage and repair were demonstrated in a comet assay. There was a noticeable reduction in (LC) development. Experiments underlying the anti-cancer activity of (EACR) on (A549) cells may be regarded as one of the most promising targets for novel cancer treatment strategies (LC).","PeriodicalId":9156,"journal":{"name":"Bioscience Biotechnology Research Communications","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43558898","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biological and Computational Approach to Modify Bacterial Size and Reduce its Antibiotic Consumption Targeting Mreb Bacterial Cytoskeletal Protein 以Mreb细菌细胞骨架蛋白为目标，改变细菌大小和减少抗生素消耗的生物学和计算方法

Bioscience Biotechnology Research Communications Pub Date : 2022-03-25 DOI: 10.21786/bbrc/15.1.11

P. J.

{"title":"Biological and Computational Approach to Modify Bacterial Size and Reduce its Antibiotic Consumption Targeting Mreb Bacterial Cytoskeletal Protein","authors":"P. J.","doi":"10.21786/bbrc/15.1.11","DOIUrl":"https://doi.org/10.21786/bbrc/15.1.11","url":null,"abstract":"Amongst the cytoskeletal proteins of bacteria, MreB is known to have very crucial role in modulating shape of the bacteria. Present study involves the use of biocide (A-22) which minimizes the bacterial size augmenting with minimal antibiotic consumption. Intended experiment is designed to be carried out on selected pure strains of gram-positive and gram-negative bacteria namely Lactobacillus rhamnosous ATCC 7469 and Pseudomonas aeruginosa ATCC 27853 respectively. The pure strains are exposed to biocide and changes in the shape is recorded by means of Foldscope (Origami based paper microscope, Prakash Labs) and in-vivo assessment done using antibiotic sensitivity assays with different antibiotics.The novel biocide specifically targeting bacterial cytoskeletal protein, that determines rod shape among bacterial population. The said compound is also experimented as combinational drug along with conventional antibiotics to reduce antibiotic dose needed to kill and to overcome antibiotic resistance. The A-22has reduced nearly 60-70% antibiotic usage. In Pseudomonas aeruginosa ATCC 27853 when tested for MIC using A-22 and different antibiotics, it was found that 0.5 µg/ml of ampicillin, 1 µg/ml of streptomycin and 5 µg/ml erythromycin were effective in curtailing bacteria against conventional antibiotic concentrations ampicillin 128 µg/ml streptomycin 32 µg/ml, erythromycin 64 µg/ml. Compared to doses of antibiotics required to kill bacteria, the combinational drug of biocide and antibiotic have shown promising effects in killing bacteria at very less concentration, this can useful for treating most diseases caused by antimicrobial resistance bacterial populations.","PeriodicalId":9156,"journal":{"name":"Bioscience Biotechnology Research Communications","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44681779","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0