{"title":"Standardization and Evaluation of Buffers: A One Step DNA Extraction Protocol from Microbial Cultures","authors":"Poornima Shyam","doi":"10.21786/bbrc/16.2.6","DOIUrl":"https://doi.org/10.21786/bbrc/16.2.6","url":null,"abstract":"Bacteria are one of the most diverse organisms that exist. Every day labs are flooded with cultures to detect the type of microorganisms and their antibiotic resistance patterns to treat patients. However, this reporting takes 3 days. Molecular biology is a field that is associated with quicker reporting. This work is an attempt to make molecular testing easier and quicker to help with one step DNA extraction method. 3 different buffers were tested for DNA extraction from cultures. The result of the best buffer is represented in agarose gel electrophoresis. Efficient extraction of DNA from bacteria was seen. The first step to standardization of buffers for the one-step extraction of DNA from bacteria. The buffers need to be tested and standardized with a wider variety of samples of both gram-positive and gram-negative organisms and an efficient method of extraction from the primary samples instead of the culture plates has to be standardized. This is a pilot study to screen the buffers for their effectiveness.","PeriodicalId":9156,"journal":{"name":"Bioscience Biotechnology Research Communications","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41548319","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Didactic Strategy for the Use of Virtual Itineraries in the Training of Graduates in Education. Geography","authors":"Oliday Aguilar Espinosa","doi":"10.21786/bbrc/16.2.7","DOIUrl":"https://doi.org/10.21786/bbrc/16.2.7","url":null,"abstract":"The training of teachers has always been a necessity of the planet aligned to the space and time where this process takes place. Future training of graduates in education, Geography who study geographic space need novel ways to appropriate these contexts and knowledge. The objective of this research was to propose a didactic strategy with the use of virtual didactic itineraries, in the teaching-learning process for the training of education, Geography in the first year of the career: Bachelor of Education. Geography, through the subject Introduction to Labor Practice, which contributed to deepen the knowledge about different landscapes and at the same time developed professional skills related to the study of the landscape. For the development of the research, information collection methods were used (participatory observation, document review, interview, expert criteria) and information processing methods (analysis – synthesis, inductive-demonstrative, logical history, descriptive statistics, modeling and analysis). The methods allowed to determine the needs of the students from the cognitive, educational and procedural point of view, regarding the teaching-learning process. The didactic strategy was implemented in practice, achieving very satisfactory results from the 2019-2022 academic years. It allows contributing to the development of professional pedagogical skills while deepening the knowledge of different landscapes in the students of the Degree in Education. Geography of the Faculty of Secondary Education of the Central University “Marta Abreu” of Las Villas, Villa Clara, Cuba.","PeriodicalId":9156,"journal":{"name":"Bioscience Biotechnology Research Communications","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47923869","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Predictive Modeling of COVID- 19 Confirmed Cases Using Regressive Objective Regression Methodology","authors":"Fernando Martínez Fernández","doi":"10.21786/bbrc/16.2.1","DOIUrl":"https://doi.org/10.21786/bbrc/16.2.1","url":null,"abstract":"The use of predictive models for the evolution of the pandemic is of great help in decision-making by the authorities. The fundamental objective of this work was to obtain through the Regressive Objective Regression, predictions of confirmed cases of COVID-19 in the Marta Abreu Teaching Polyclinic of the city of Santa Clara. In short-term modeling the model was significant at 19.7% with an error of 0.12. Variables dichotommics, saw tooth and saw tooth inverted and risk returned in 1.3, and 12 cases the trend is negative and not significant. We can conclude that a perfect result was obtained in the long term with the ROR methodology. The short-term ROR model depends on the cases of COVID-19 in the previous case, 3 cases back and 12 cases back without significant trend. The long-term model is perfect and depends on the cases of COVID-19 in 12 cases ago, with a negative trend.","PeriodicalId":9156,"journal":{"name":"Bioscience Biotechnology Research Communications","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47914790","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Avifaunal Abundance of Lumding Forest Reserve Area, Assam, India","authors":"J. Arjun","doi":"10.21786/bbrc/16.2.5","DOIUrl":"https://doi.org/10.21786/bbrc/16.2.5","url":null,"abstract":"The solitary aim of our investigation was to explore the avian species richness in Lumding Forest Reserve area, Assam.The survey was cooperated by the team of Zoological Survey of India. After extended survey, 146 species of birds were spotted from Lumding Forest Reserve area, where birds from 17 different orders (Passeriformes, Columbiformes, Accipitriformes , Piciformes, Bucerotiformes ,Gruiformes, Apodiformes, Coraciiformes, Trogoniformes, Cuculiformes, Galliformes, Ciconiiformes, Suliformes , Pelecaniformes , Strigiformes, Anseriformes, Charadriiformes) and 76 families were put down. The bird species thus obtained were compared with the IUCN Red list from where interesting cum astonishing facts were acquired. The IUCN Red list informed us that, 92.51% bird species spotted were under Least Concern category, where as 3.4% were Near Threatened, 2.72% were Vulnerable, 0.68% were Critically Endangered and Endangered. We considered ourselves successful for obtaining such astouned statistics about the avian species plentitude of Lumding Forest Reserve, which will provoke other researchers to come forward and explore more about this place.","PeriodicalId":9156,"journal":{"name":"Bioscience Biotechnology Research Communications","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45149508","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Localization of NPY Immunoreactivity in the Proximal and Distal Intestinal Region of Teleost Fish, Notopterus","authors":"T. Khadse","doi":"10.21786/bbrc/16.2.8","DOIUrl":"https://doi.org/10.21786/bbrc/16.2.8","url":null,"abstract":"The purpose of the present study was to investigate the immunocytochemical aspects of the intestine of the teleost fish Notopterus notopterus. The distribution and relative frequency of the endocrine cells in the intestine were studied immunocytochemically using Streptavidin biotin peroxidase complex method. The aim was analyse the distribution and localization of immunoreactivity in the intestine of Notopterus notopterus against the antisera NPY. The samples were taken from the proximal and distal intestine. The NPY immunoreactive endocrine cells were found in the distal intestine at low frequencies than proximal intestine. These immunoreactive cells were distributed among more in mucosa epithelium and very low in the lamina propria and submucosa of intestine of N.notopterus.","PeriodicalId":9156,"journal":{"name":"Bioscience Biotechnology Research Communications","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43607427","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Ecology of Biofouling Phytoplankton in Chinnamuttom Harbour Waters Southeast Coast of India","authors":"P. Nithya","doi":"10.21786/bbrc/16.2.9","DOIUrl":"https://doi.org/10.21786/bbrc/16.2.9","url":null,"abstract":"Phytoplankton are the primary source of food, attributed most zooplankton communities and larval stages, and many meroplankton such as bivalves, crustaceans, and fishes. In the present study, phytoplankton samples were collected from surface water and substratum of the ship hull during the period of June 2015 to May 2016. Population density recorded from water and substratum of the ship hull ranged from 1.5e+4 to 3.4e+4 cells/L. Shannon-Wiener diversity index (H‛) of station I and station II ranged from 5.448 to 6.134 bits/ind. Simpson’s richness range was 0.973 to 0.984 at station I and station II. Pielou’s Evenness index (J’) was found to be from 0.950 to 0.979 and Chlorophyll ‘a’ range was 0.277 to 1.326 mg/m3 in the station I and station II. During this entire study period, 74 species of phytoplankton and 54 species of phytoplankton in station II have been recorded. Data of the present study show that significant differences occurred in over all percentage composition of phyhtoplankton species, both in in water and substratum. In water samples, recorded data showed that 85% were of Bacillariophyceae, 8 % of Chlorophyceae and, 7% of Dinophyceae. On the other hand, 75 % Bacillariophyceae, 7 % Chlorophyceae, 16% Dinophyceae and 2% Cyanophyceae were recorded in the substratum. The marine biofouling phytoplankton density, diversity, richness, evenness and chlorophyll’a’ were recorded in their maximum levels during summer season, and minimum were obtained in the monsoon. The diatoms were the predominant groups among the phytoplankton. Among the diatoms, species like Nitzschia longissima, Coscinodiscus centralis and Fragilaria sp. were record almost in all the seasons in the present study.","PeriodicalId":9156,"journal":{"name":"Bioscience Biotechnology Research Communications","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43935507","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Adsorption of Phenol and Resorcinol on Parthenium Based Activated Carbon (Pac) in Basal Salt Medium: Equilibrium and Kinetics","authors":"Khushi Gandhi","doi":"10.21786/bbrc/16.2.4","DOIUrl":"https://doi.org/10.21786/bbrc/16.2.4","url":null,"abstract":"The adsorption studies were carried out for the removal of Phenol and Resorcinol on Parthenium Based Activated Carbon (PAC) at 301 K and neutral pH ~7. To determine the adsorption equilibrium isotherms, the experiments were performed at the initial concentration of adsorbates with adsorbent doses from 1 g/l to 10 g/l. The experimental data were fitted to six isothermal models, and a nonlinear least square method has been used to estimate model parameters. It has been observed that for adsorption on PAC, Freundlich model was best fitted for the study and data was more closely related for the removal of both the pollutants from industrial wastewater. In this study, the adsorption kinetics were also studied in three distinct phases-rapid, medium & amp: slow for the time period till 12 hours. The parameter values found in this study shall be useful in designing and activated carbon adsorber for treatment of industrial wastewater at large scale. The present work shows that Parthenium based activated carbon (PAC) was an effective method for the adsorption of phenol and resorcinol and p-cresol from aqueous solution. The adsorption of phenol and its derivatives are in the following order: Phenol is adsorbed in maximum amount Resorcinol is comparatively adsorbed lesser than phenol.","PeriodicalId":9156,"journal":{"name":"Bioscience Biotechnology Research Communications","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41950761","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Knowledge and management Assessment of Temporomandibular Joint (TMJ) Disorders Among Dental Interns at King Saud University","authors":"H. Albagieh","doi":"10.21786/bbrc/16.2.10","DOIUrl":"https://doi.org/10.21786/bbrc/16.2.10","url":null,"abstract":"The temporomandibular joint (TMJ) complex is mainly composed of bone, cartilage, muscles, ligaments, and neurovascular channels that supply the tissues. The temporomandibular disorders (TMD) are collective term embracing a number of clinical problems that involve the masticatory muscles, the temporomandibular joints (TMJs) and associated structures, or both. The most common symptoms of temporomandibular joint disorders (TMDs) are pain, noises in the joint, limitation of mouth opening, or a combination of these. The objective of this study was to assess the knowledge and management of temporomandibular joint (TMJ) disorders among dental interns at King Saud University, Saudi Arabia. The sample size of this study consisted of 100 dental interns at King Saud University. Informed consent from the participants was obtained. The participants were informed that their responses and personal information would be kept confidential. A cross-sectional survey was prepared in English language using Google Forms. The survey contains 12 questions was distributed among the selected sample of dental interns at King Saud University. The average knowledge score of the dental interns on the knowledge regarding management of (TMJ) disorders was 4.04 (SD = 1.42). The maximum score obtained was 8 out of 8, the minimum was zero. that the majority of the dental interns (67%) had fair knowledge. The finding of the present study showed, there was a fair level of knowledge about (TMJ) disorders among dental interns. Many interns are unable to diagnose and treat patients suffering from temporomandibular joint disorders.","PeriodicalId":9156,"journal":{"name":"Bioscience Biotechnology Research Communications","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45927800","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"BMI for Age Percentile As Criteria for Assessment of Obesity in Children and Adolescents","authors":"M. Dewan","doi":"10.21786/bbrc/16.2.3","DOIUrl":"https://doi.org/10.21786/bbrc/16.2.3","url":null,"abstract":"The most useful population-level measure of overweight and obesity is BMI. This measure is same for men and women of all ages. But it should not be thought to be as final factor to calculate the same body fat percentage in different individuals. Various proposed reference values for overweight and obesity exist for BMI, but the combined effect of BMI and BMI for age percentile recommendations is scarce. A total 2048 children participated in the epidemiological survey. According to Body Mass Index in the study, 66.26% subjects were found to be malnourished in the total population. The degree of obesity had been observed as Pre-obese 3.81% and Obese-I 3.81% and Obese-II were 0.88%. Pre-obese, obese-I and obese-II boys were more than the girls. According to BMI-for-age percentile, overall, 6.20% of children and adolescents were at risk of overweight and 4.39% were overweight during health survey while 24.80% are underweight.","PeriodicalId":9156,"journal":{"name":"Bioscience Biotechnology Research Communications","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45235090","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Potential Application of the Objective Regression Regressive Methodology","authors":"R. F. Duarte","doi":"10.21786/bbrc/16.1.9","DOIUrl":"https://doi.org/10.21786/bbrc/16.1.9","url":null,"abstract":"The possibility of having a methodology that allows the modeling and prediction, in the short, medium and long term, of biological, social and natural disaster processes and/or phenomena is something great. The objective of the research consisted in demonstrating the potentialities and real capacity of application of the methodology of the Regressive Objective Regression (ROR) in the different fields and branches of scientific research. In the ORR methodology, in a first step, dichotomous variables DS, DI and NoC are created. Then, the module corresponding to the Regression analysis of the SPSS statistical package (ENTER method) is executed, where the predicted variable and the ERROR are obtained; subsequently, the autocorrelograms of the ERROR variable are obtained, paying attention to the maximum of the significant partial autocorrelations, and the new variables are calculated according to the significant Lag of the PACF. Finally, these regressed variables are included in the new regression in a process of successive approximations until a white noise is obtained. Wide possibilities of modeling and forecasting in the short, medium and long term, which go beyond the modeling of infectious entities of parasitic and viral etiology, Acute Respiratory Infections, Acute Bronchial Asthma crises, forecasting of extreme meteorological disturbances, prediction of latitude and longitude of earthquakes, modeling of climatic variables, and even the own electric consumption of a municipality, province and nation. The ROR methodology has demonstrated potential and real capabilities of application in dissimilar fields and branches of science, so it is a novel contribution to the science of modeling and forecasting of variables to know the future, as well as the impact that different variables contribute to an event or phenomenon, and being universal, it can be applied anywhere in the universe.","PeriodicalId":9156,"journal":{"name":"Bioscience Biotechnology Research Communications","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44821779","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}