Standardization and Evaluation of Buffers: A One Step DNA Extraction Protocol from Microbial Cultures

Abstract

Bacteria are one of the most diverse organisms that exist. Every day labs are flooded with cultures to detect the type of microorganisms and their antibiotic resistance patterns to treat patients. However, this reporting takes 3 days. Molecular biology is a field that is associated with quicker reporting. This work is an attempt to make molecular testing easier and quicker to help with one step DNA extraction method. 3 different buffers were tested for DNA extraction from cultures. The result of the best buffer is represented in agarose gel electrophoresis. Efficient extraction of DNA from bacteria was seen. The first step to standardization of buffers for the one-step extraction of DNA from bacteria. The buffers need to be tested and standardized with a wider variety of samples of both gram-positive and gram-negative organisms and an efficient method of extraction from the primary samples instead of the culture plates has to be standardized. This is a pilot study to screen the buffers for their effectiveness.