Journal of stem cell research & therapeutics最新文献_第8页

Rhabdomyolysis after Transplantation: Case Report after Allogeneic Hematopoietic Transplantation and Review of Literature 移植后横纹肌溶解:异基因造血移植一例报告及文献复习

Journal of stem cell research & therapeutics Pub Date : 2017-01-25 DOI: 10.15406/jsrt.2017.2.00055

S. Leotta, C. Pirosa, A. Cupri, E. Martino, A. Spadaro, Milone Giuseppe

引用次数: 0

Utilizing chick embryo in ovo model system to study the effects of misoprostol on early embryogenesis - a pilot study 利用鸡胚卵模型系统研究米索前列醇对早期胚胎发生的影响

Journal of stem cell research & therapeutics Pub Date : 2017-01-24 DOI: 10.15406/JSRT.2017.02.00054

Nusrat Zareen, N. Karim, Lubna Akhtar, J. Faisal

引用次数: 0

A2 erythrocytes lack a antigen modified glycoproteins which are present in A1 erythrocytes A2红细胞缺乏存在于A1红细胞中的抗原修饰糖蛋白

Journal of stem cell research & therapeutics Pub Date : 2017-01-18 DOI: 10.15406/jsrt.2017.02.00053

E. Gehrie, P. Young

引用次数: 3

Stem Cells-Based Therapeutics for Parkinson’s Disease - A Transcriptomic Analyses During Dopaminergic Neuron Differentiation under 3- and 2- Dimensional Environments using Human Embryonic Stem Cells 基于干细胞的帕金森病治疗——利用人类胚胎干细胞在三维和二维环境下进行多巴胺能神经元分化的转录组学分析

Journal of stem cell research & therapeutics Pub Date : 2017-01-17 DOI: 10.15406/jsrt.2017.02.00052

Jaemin Kim, P. Sachdev, P. Zhang, K. Sidhu

{"title":"Stem Cells-Based Therapeutics for Parkinson’s Disease - A Transcriptomic Analyses During Dopaminergic Neuron Differentiation under 3- and 2- Dimensional Environments using Human Embryonic Stem Cells","authors":"Jaemin Kim, P. Sachdev, P. Zhang, K. Sidhu","doi":"10.15406/jsrt.2017.02.00052","DOIUrl":"https://doi.org/10.15406/jsrt.2017.02.00052","url":null,"abstract":"Parkinson’s disease (PD) is a neurodegenerative disease which is caused by many factors including progressive degeneration of dopamine (DA)-secreting neurons which reside in the midbrain substantia nigra compacta (SNc). Current available treatments comprise of intake of DA replenishing drugs or implantation of electrical impulse device. However, the short-term effect of the treatments and risks of side effects haveseverely limited the widespread application of thesetherapiesfor all patients with PD.Hence, human embryonic stem cells (hESCs), which are capable of both self renewal and differentiation into all cell types of human body, could potentially provide a renewable source of surrogate DA neurons for transplantation into PD patients. One of the challenges in using hESCs therapeutically is the establishment of protocols that could effectively direct their differentiation into functionalDA neurons. A specific investigation on the derivation of DA neurons was carried out by usinga three-dimensional (3D)environmentsuch as encapsulation. Characterizationstudyby microarray wasperformed to analyze the global expression profile in 3D-derived DA neurons after 28 days of differentiation. In comparison to the samples of DA neuronal differentiated hESCs under 2D platform for 28 days, the analysis detected the reduced expression of gene that are involved in pluripotency or mitosis but increased expression of genes that are involved in neuronal developments such as Wnt, hedgehogand mitogen-activated protein kinase (MAPK) signaling pathway. The results suggest that the 3D differentiation system may have affected the regulatory or signalling mechanisms which enhanced the rate of differentiation towards ectoderm.","PeriodicalId":91560,"journal":{"name":"Journal of stem cell research & therapeutics","volume":"5 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2017-01-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84885759","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Marker profiles of human endometrial stem cells at various passages cultured in-vitro 人子宫内膜干细胞在体外不同传代培养中的标记谱

Journal of stem cell research & therapeutics Pub Date : 2017-01-11 DOI: 10.15406/JSRT.2017.02.00051

P. Kaingade, A. Nikam, Sachin Kulkarni, I. Somasundaram

{"title":"Marker profiles of human endometrial stem cells at various passages cultured in-vitro","authors":"P. Kaingade, A. Nikam, Sachin Kulkarni, I. Somasundaram","doi":"10.15406/JSRT.2017.02.00051","DOIUrl":"https://doi.org/10.15406/JSRT.2017.02.00051","url":null,"abstract":"Endometrium is a dynamic organ, which undergoes extensive proliferation and regeneration throughout reproductive cycle of women. Hence, extensive research on adult stem cells of endometrium is underway. An easy approach to identify stem cells is its marker characterization. However, does these markers behave same in all conditions of stem cells in-vitro is uncertain? Hence, the main objective of the study is to check if mesenchymal stem cells of the endometrium (eMSCs) could retain its marker characterization at various passages in-vitro. \u0000 \u0000 \u0000 \u0000 The study design was accomplished as follows: The endometrial tissue (n=10) were collected from reproductively active women with a mean age of 35±1.5 and body mass index of 24±1.4, undergoing D&C/hysterectomy. Samples were collected in a sterile manner. The plastic, adherent mesenchymal stem cells obtained from culturing of the cells isolated from endometrial tissue (eMSCs) are characterized for study design was accomplished by characterizing some of the markers such as CD44, CD166, CD106, CD49d, CD31, CD54, CD34, CD117, CD90, CD105, CD73, CD140b, ABCG2 of eMSCs at various passages (P1, P3, P5 & P10) in-vitro using flowcytometry. The obtained results were analysed statistically using student’s t-test and significant results are discussed. \u0000 \u0000 The study identified tissue-specific markers of eMSCs, where the percentage expression of most of the markers was consistently similar at P3 and P10, expect for CD49d and CD54, which showed a reduction pattern and ABCG2, which showed an increase in percentage at P10 as compared to P3. This article reveals the significance of in-depth marker characterization of human endometrial stem cells in-vitro.","PeriodicalId":91560,"journal":{"name":"Journal of stem cell research & therapeutics","volume":"20 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2017-01-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85288262","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Stem Cell and Toxicology 干细胞与毒理学

Journal of stem cell research & therapeutics Pub Date : 2017-01-11 DOI: 10.15406/jsrt.2017.02.00050

D. Morsi

引用次数: 0

Stem cell treatment after ischemic stroke alters the expression of dna damage signaling molecules 缺血性中风后的干细胞治疗改变了dna损伤信号分子的表达

Journal of stem cell research & therapeutics Pub Date : 2016-12-30 DOI: 10.15406/JSRT.2016.01.00049

B. Chelluboina, Koteswara Rao Nalamolu, J. Klopfenstein, David Z. Wang, K. Veeravalli

{"title":"Stem cell treatment after ischemic stroke alters the expression of dna damage signaling molecules","authors":"B. Chelluboina, Koteswara Rao Nalamolu, J. Klopfenstein, David Z. Wang, K. Veeravalli","doi":"10.15406/JSRT.2016.01.00049","DOIUrl":"https://doi.org/10.15406/JSRT.2016.01.00049","url":null,"abstract":"Accumulating evidence suggests that oxidative DNA damage plays a critical role in cell death associated with ischemic stroke. Endogenous oxidative DNA damage can be detected in the ischemic brain during the stages that precedes the manifestation of cell death and is believed to trigger cell death via various intracellular signaling pathways. Inhibiting the signaling associated with DNA damage induction or facilitating the signaling associated with the DNA repair process can be neuroprotective against brain injury after ischemic stroke. Recent reports demonstrated that human umbilical cord blood-derived mesenchymal stem cells (HUCB-MSCs) prevented the upregulation of apoptotic signaling pathway molecules and thereby attenuated the extent of apoptosis after focal cerebral ischemia as well as improved the neurological recovery. Therefore, we hypothesized that HUCB-MSCs treatment after focal cerebral ischemia prevents the overexpression of molecules associated with DNA damage induction as well as augments the expression of molecules associated with DNA repair process. In order to test our hypothesis, we administered HUCB-MSCs (0.25x106cells/animal) intravenously via tail vein to male Sprague-Dawley rats that were subjected to a two-hour middle cerebral artery occlusion followed by one-day reperfusion. Ischemic brain tissues obtained from various groups seven days’ post reperfusion were subjected to DNA damage signaling pathway PCR microarray. Our results demonstrated the induction of both DNA damage inducing and repair genes after focal cerebral ischemia and reperfusion. HUCB-MSCs treatment downregulated the DNA damage inducing genes and upregulated the DNA repair genes without disturbing the endogenous defense mechanisms.","PeriodicalId":91560,"journal":{"name":"Journal of stem cell research & therapeutics","volume":"29 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2016-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89588686","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Inherent properties of extracellular vesicles collected from re-differentiated cancer stem cells 从再分化的癌症干细胞中收集的细胞外囊泡的固有特性

Journal of stem cell research & therapeutics Pub Date : 2016-12-30 DOI: 10.15406/JSRT.2016.01.00048

Rajeswari Jinka, Avinash Raj, S. Gunda, P. Gopal

引用次数: 0

Human embryonic stem cells derived mesenchymal stem/stromal cells and their use in regenerative medicine 人胚胎干细胞衍生的间充质干细胞/基质细胞及其在再生医学中的应用

Journal of stem cell research & therapeutics Pub Date : 2016-12-30 DOI: 10.15406/JSRT.2016.01.00047

F. Akyash, Mahdieh Javidpou, F. S. Nodoushan, B. Aflatoonian

引用次数: 5

A comprehensive guide for the stem cell research 干细胞研究的综合指南

Journal of stem cell research & therapeutics Pub Date : 2016-12-29 DOI: 10.15406/jsrt.2016.01.00046

Vinita Thakur

{"title":"A comprehensive guide for the stem cell research","authors":"Vinita Thakur","doi":"10.15406/jsrt.2016.01.00046","DOIUrl":"https://doi.org/10.15406/jsrt.2016.01.00046","url":null,"abstract":"Stem cells are undifferentiated cells classified into two groups, a) stem cells from embryos formed during the blastocyst phase of an embryological development and b) stem cells of an adult. Embryonic stem cells are derived from a 4-5 day old human embryo that is in the blastocyst phase of a development [1]. Adult stem cells exist throughout the body after embryonic development and are found inside of different types of tissues. Adult bone marrow and fat have stem cells along with other tissues [2]. Apart from these two types there is also embryonic germ cells which are collected from the fetus later in developmental process from gonadal ridge [3]. Adult stem cell serve as fresh source of cells in living organism They replace the cells that need to be replaced on regular basis like blood and other connective tissue [4]. Stem cell technology is rapidly growing in the field of regenerative medicine [5]. According to Patil AM, stem cell research has offered a new viable therapeutic option for degenerative diseases, injuries and other diseased condition. Stem cells are called as master cells with ability to grow into more than 200 cell types [6,7]. Based on their capacity to divide and differentiate they may be totipotent, pluripotent or multipotent [5].","PeriodicalId":91560,"journal":{"name":"Journal of stem cell research & therapeutics","volume":"19 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2016-12-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81119699","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0