Inherent properties of extracellular vesicles collected from re-differentiated cancer stem cells

The focus on Stem cells provides a considerable attention in translational medicine for their potential capacity in terms of tissue regeneration, repair and as drug delivery. Recent evidences showed that stem cells secrete small vesicles into the extracellular milieu, known as extracellular vesicles (EVs). They are known to carry a repertoire of mRNAs, miRNAs, DNA, proteins, and lipids that can be transferred to neighboring cells, modifying their phenotype as well as the microenvironment. Stem cell-Extracellular vesicles may provide the opportunity to overcome the hurdles or complications raised in stem cell therapeutic strategies. Our data showed that the extracellular vesicles secreted by the spheroids, constituted by the stem cells obtained by the dedifferentiation process are positive for CD133 and negative for the multi-drug resistance and tumorigenecity.