Journal of interdisciplinary nanomedicine最新文献

{"title":"The anticoagulant properties of cadmium telluride quantum dots","authors":"Ciarán M. Maguire,&nbsp;Michelle Lavin,&nbsp;Mairead Doyle,&nbsp;Mary Byrne,&nbsp;Adriele Prina-Mello,&nbsp;James S. O'Donnell,&nbsp;Yuri Volkov","doi":"10.1002/jin2.35","DOIUrl":"10.1002/jin2.35","url":null,"abstract":"<p>The size-dependent optical properties of quantum dots (QDs) are frequently exploited for use in medical imaging and labelling applications. Similarly, presented here, they also elicit profound size-dependent anticoagulant properties. Cadmium telluride quantum dot (QDs) (3.2 nm) were shown to have a dramatic anticoagulant effect centred on around the intrinsic coagulation pathway, compared to their 3.6 nm counterparts. Several clinically relevant diagnostic tests were carried out over a concentration range of the QDs and demonstrated that the 3.2 nm QDs elicited their response on the intrinsic pathway as a whole, yet the activity of the individual intrinsic coagulation factors was not affected. The mechanism appears also to be strongly influenced by the concentration of calcium ions and not cadmium ions leached from the QDs. Static and shear-based primary haemostasis assays were also carried out, demonstrating a profound anticoagulant effect which was independent of platelets and phospholipids. The data presented here suggest that the physical–chemical properties of the QDs may have a role in the modulation of haemostasis and the coagulation cascade, in a yet not fully understood mechanism. This study has implications for the use of similar QDs as diagnostic or therapeutic tools in vivo<i>,</i> and for the occupational health and safety of those working with such materials.</p>","PeriodicalId":91547,"journal":{"name":"Journal of interdisciplinary nanomedicine","volume":"3 1","pages":"16-28"},"PeriodicalIF":0.0,"publicationDate":"2018-04-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1002/jin2.35","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36253677","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification of regulatory needs for nanomedicines","authors":"Susanne Bremer-Hoffmann,&nbsp;Blanka Halamoda-Kenzaoui,&nbsp;Sven Even Borgos","doi":"10.1002/jin2.34","DOIUrl":"10.1002/jin2.34","url":null,"abstract":"<p>The application of nanotechnology in health care is widely accepted as a potential driver of biomedical innovation. By exploiting their unique physicochemical properties, nanomedicines can monitor, repair, and control biological systems in order to address diseases for which currently no or only insufficient diagnostic and therapeutic tools are available. Nevertheless, the opportunities of nanotechnologies in the health sector are accompanied by challenges in the regulation of these products. Sufficient knowledge on their quality, safety, and efficacy must be gained and standardised methods must be made available to support the regulatory decision making and allow a smooth translation towards clinical applications. We have conducted a survey among regulatory authorities with the aim to obtain a general overview on the status and regulatory needs of nanomedicines and to indicate some trends on future requirements. The outcome has demonstrated strong regional differences in the regulation of nanomedicines and confirmed the need for the harmonisation of information requirements on nano-specific properties. In addition, a number of critical physicochemical properties that have already been proposed in the scientific literature were verified in the survey as relevant for regulatory decision making. Finally, the survey has demonstrated an interest of regulatory agencies in an independent nanomedicine characterisation facility that can support regulators in the evaluation of these systems and at the same time assess the performance of existing and new test methods for their application to the field of nanomedicine.</p>","PeriodicalId":91547,"journal":{"name":"Journal of interdisciplinary nanomedicine","volume":"3 1","pages":"4-15"},"PeriodicalIF":0.0,"publicationDate":"2018-03-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1002/jin2.34","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42656709","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bombesin-functionalized water-soluble gold nanoparticles for targeting prostate cancer","authors":"Emily J. Simpson,&nbsp;Pierangelo Gobbo,&nbsp;Fernanda C. Bononi,&nbsp;Emily Murrell,&nbsp;Mark S. Workentin,&nbsp;Leonard G. Luyt","doi":"10.1002/jin2.33","DOIUrl":"10.1002/jin2.33","url":null,"abstract":"<p>Cancer targeting can be used for both tumor diagnosis and therapy. Recently, gold nanoparticles (AuNPs) have found utility in this field as they are very small in size, and thus display an enhanced permeability and retention effect, allowing them to be taken up by tumor cells through “passive targeting.” However, this accumulation is non-specific. Conversely, AuNPs functionalized with targeting entities such as peptides, antibodies, or small molecules can specifically target tumors through interaction with cancer-specific protein receptors. In this study, targeted AuNPs were developed using an azide-modified peptide that was able to react with alkyne-functionalized AuNPs through an interfacial strain-promoted azide-alkyne cycloaddition. Small (3 nm) AuNPs were made water-soluble through PEGylation and functionalized with dibenzocyclooctyne to add the alkyne functionality. For the targeting entity, a pan-bombesin peptide ([D-Phe⁶,β-Ala¹¹,Phe¹³,Nle¹⁴]bombesin(6–14)) was chosen as it binds to all four receptor subtypes of the gastrin releasing peptide receptor, which is highly expressed in prostate cancer. Prostate cancer (PC-3) cells were incubated with the targeted AuNPs and studied via transmission electron microscopy. AuNPs conjugated with bombesin showed higher accumulation in PC-3 cells than either the blocking or control studies. These results suggest that these small, water-soluble, bombesin-functionalized AuNPs have potential applications in targeting prostate cancer as diagnostic or therapeutic entities.</p>","PeriodicalId":91547,"journal":{"name":"Journal of interdisciplinary nanomedicine","volume":"2 4","pages":"174-187"},"PeriodicalIF":0.0,"publicationDate":"2017-12-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1002/jin2.33","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43639154","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Intracellular delivery of nano-formulated antituberculosis drugs enhances bactericidal activity","authors":"Samantha Donnellan,&nbsp;Vicki Stone,&nbsp;Helinor Johnston,&nbsp;Marco Giardiello,&nbsp;Andrew Owen,&nbsp;Steve Rannard,&nbsp;Ghaith Aljayyoussi,&nbsp;Benjamin Swift,&nbsp;Lang Tran,&nbsp;Craig Watkins,&nbsp;Karen Stevenson","doi":"10.1002/jin2.27","DOIUrl":"10.1002/jin2.27","url":null,"abstract":"<div>\u0000 \u0000 <p>Tuberculosis kills more people worldwide than any other infectious disease. Treatment requires multiple drug therapy administered over long periods (6–24 months). The emergence of multidrug-resistant strains is a major problem, and with few new drugs in the pipeline, a novel modus operandi is urgently required. Solid drug nanoparticles (SDNs), a new development in nanomedicine, offer a fresh therapeutic approach. Here, we show that SDNs are more effective (50-fold) at killing pathogenic mycobacteria than aqueous forms of the same drug and can target mycobacteria internalised by macrophages, where bacilli reside. We demonstrate synthesis of dual and triple drug loaded SDNs, facilitating combination tuberculosis therapy. Our results suggest that by employing SDNs of existing antibiotics, it may be possible to improve drug delivery and therefore reduce drug dosage to lessen side effects and fight drug resistance.</p>\u0000 </div>","PeriodicalId":91547,"journal":{"name":"Journal of interdisciplinary nanomedicine","volume":"2 3","pages":"146-156"},"PeriodicalIF":0.0,"publicationDate":"2017-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1002/jin2.27","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46435356","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In vitro characterisation of solid drug nanoparticle compositions of efavirenz in a brain endothelium cell line","authors":"Paul Curley,&nbsp;Marco Giardiello,&nbsp;Neill J. Liptrott,&nbsp;David Dickens,&nbsp;Darren M. Moss,&nbsp;James J. Hobson,&nbsp;Alison C. Savage,&nbsp;Tom O. McDonald,&nbsp;Marco Siccardi,&nbsp;Steve Rannard,&nbsp;Andrew Owen","doi":"10.1002/jin2.32","DOIUrl":"10.1002/jin2.32","url":null,"abstract":"<p>The antiretroviral drug efavirenz displays many desirable pharmacokinetic properties such as a long half-life enabling once daily dosing but suffers from central nervous system safety issues. Various nanotechnologies have been explored to mitigate some of the limitations with efavirenz. While there has been progress in increasing the bioavailability, there has been no attempt to assess the impact of increased exposure to efavirenz on central nervous system safety. The uptake of aqueous and solid drug nanoparticle (SDN) formulations of efavirenz was assessed in the human cerebral microvessel endothelial cells/D3 brain endothelial cell line. The mechanisms of uptake were probed using a panel of transport and endocytosis inhibitors. The cellular accumulation of an efavirenz aqueous solution was significantly reduced by amantadine, but this was not observed with SDNs. The uptake of efavirenz SDNs was reduced by dynasore, but concentrations of the efavirenz aqueous solution were not affected. These data indicate that efavirenz is a substrate for transporters in brain endothelial cells (amantadine is an inhibitor of organic cation transporters 1 and 2), and formation of SDNs may bypass this interaction in favour of a mechanism involving dynamin-mediated endocytosis.</p>","PeriodicalId":91547,"journal":{"name":"Journal of interdisciplinary nanomedicine","volume":"2 3","pages":"157-169"},"PeriodicalIF":0.0,"publicationDate":"2017-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1002/jin2.32","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48775210","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Green synthesis of silver nanoparticles using Alysicarpus monilifer leaf extract and its antibacterial activity against MRSA and CoNS isolates in HIV patients","authors":"Muthupandi Kasithevar,&nbsp;Muthupandian Saravanan,&nbsp;Periyakaruppan Prakash,&nbsp;Hema Kumar,&nbsp;Muhammad Ovais,&nbsp;Hamed Barabadi,&nbsp;Zabta Khan Shinwari","doi":"10.1002/jin2.26","DOIUrl":"10.1002/jin2.26","url":null,"abstract":"<div>\u0000 \u0000 <p>The emergence of multi-drug-resistant microorganisms in hospital environments is a global public health problem and threat to everyone, especially HIV-infected patients. Methicillin-resistant <i>Staphylococcus aureus</i> (MRSA) and coagulase-negative <i>Staphylococci</i> (CoNS) are the major causative agents associated with morbidity and mortality in HIV patients. Therefore, control of MRSA and CoNS-related infections in HIV patients is a worldwide concern. To investigate novel, potent, and cost-effective therapeutic approaches, the current study reports a simple and rapid synthesis of silver nanoparticles (AgNPs) using aqueous leaf extract of <i>Alysicarpus monilifer</i> and its antibacterial efficacy against multi-drug-resistant MRSA and CoNS isolates from HIV patients. The green-synthesized AgNPs were characterized using ultraviolet-visible spectroscopy, transmission electron microscopy, energy dispersive X-ray analysis, selected area electron diffraction pattern, X-ray diffraction patterns, and Fourier transform infrared spectroscopy. Stable, well-defined AgNPs, mostly spherical in shape with mean size of 15 ± 2 nm, were obtained within an hour. Moreover, green synthesized AgNPs revealed significant dose-dependent antibacterial action against MRSA and CoNS isolates. This study concludes that biogenic AgNPs have demonstrated to be potent antibacterial agents in comparison with conventional antibiotics.</p>\u0000 </div>","PeriodicalId":91547,"journal":{"name":"Journal of interdisciplinary nanomedicine","volume":"2 2","pages":"131-141"},"PeriodicalIF":0.0,"publicationDate":"2017-06-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1002/jin2.26","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43756733","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The curious case of how mimicking physiological complexity in in vitro models of the human respiratory system influences the inflammatory responses. A preliminary study focused on gold nanoparticles","authors":"Dania Movia,&nbsp;Luisana Di Cristo,&nbsp;Roaa Alnemari,&nbsp;Joseph E. McCarthy,&nbsp;Hanane Moustaoui,&nbsp;Marc Lamy de la Chapelle,&nbsp;Jolanda Spadavecchia,&nbsp;Yuri Volkov,&nbsp;Adriele Prina-Mello","doi":"10.1002/jin2.25","DOIUrl":"10.1002/jin2.25","url":null,"abstract":"<div>\u0000 \u0000 <p>Environmental and biomedical nanoparticles can pose potential health risks to the human respiratory system by inducing severe lung inflammation. The aim of this case study is to present a comparison of the inflammatory response in four in vitro models of the human lung epithelium, differing by composition and/or culturing substrates, when exposed to gold nanoparticles (AuNPs). Three in vitro models of lung adenocarcinoma (A549) cells and a commercially available three-dimensional (3D) culture (MucilAir™) were tested. The models were exposed to AuNPs for 3, 6, and 24 h. AuNPs internalisation was investigated by confocal, electron microscopy, and Raman spectroscopy. Enzyme-Linked Immuno-Sorbent Assay (ELISA) was used for quantifying the secretion of the inflammatory mediator Interleukin-6 (IL-6) following exposure to AuNPs. Finally, a microfluidic approach was developed in-house to investigate whether pro-inflammatory mediators present in supernatants harvested from the AuNPs-treated cell cultures could trigger monocyte activation. Our results demonstrated that AuNPs were internalised only in submerged cultures grown on glass substrates. Nevertheless, AuNPs internalisation did not trigger a significant IL-6 secretion. Significant amounts of IL-6 were secreted by AuNPs-treated mono-cultures grown on Transwell™ inserts, triggering monocyte activation in dynamic microfluidic experiments. AuNPs did not induce IL-6 secretion in co-cultures and MucilAir™ models, although supernatants harvested from co-cultures triggered monocyte activation. Our case study demonstrates that in vitro complexity, as well as culturing substrates, deeply influence the detectable cellular responses to nanoparticles, and advocate for the adoption of more advanced tissue-mimetic cultures of the human respiratory system for nanomaterials testing.</p>\u0000 </div>","PeriodicalId":91547,"journal":{"name":"Journal of interdisciplinary nanomedicine","volume":"2 2","pages":"110-130"},"PeriodicalIF":0.0,"publicationDate":"2017-05-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1002/jin2.25","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41706105","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Abstract","authors":"","doi":"10.1002/jin2.24","DOIUrl":"10.1002/jin2.24","url":null,"abstract":"<p><b>S001</b></p><p><b>S001 The British Society for Nanomedicine</b></p><p>Speaker: Professor R. Steven Conlan</p><p><i>Swansea University Medical School, Singleton Park, Swansea, SA2 8PP, UK</i></p><p><b>Abstract</b>: With the global benefits of the new science of nanomedicine growing each year, the British Society for Nanomedicine enables open access for industry, academia, clinicians and the public to news and details of ongoing research throughout the UK. Our mission includes the direct explanation of the ongoing science and commercial developments to allow the public to understand and stay in touch with this exciting area as it impacts future global healthcare. The British Society for Nanomedicine runs the Journal of Interdisciplinary Nanomedicine (JOIN), an international peer-reviewed academic journal that aims to report truly interdisciplinary nanomedicine research. The British Society for Nanomedicine represents UK nanomedicine interests at a UK, EU and International level including the ETP nanomedicine, and European national nanomedicines platform. The British Society for Nanomedicine is proud to welcome you to ENM17 in London.</p><p><b>S002</b></p><p><b>S002 The French Society for Nanomedicine (SFNano)</b></p><p>Speaker: Dr Nathalie Mignet</p><p><i>University Paris Descartes, CNRS, Faculty of Pharmacy, Paris, France</i></p><p><b>Abstract</b>: The French Society for Nanomedicine (SFNano) is a non-lucrative association whose objectives are to favour progress and knowledge diffusion in the Nanomedicine domain. For these purposes, SFNano organises seminars, workshops and discussions in order to favour a french network, and broader, in collaboration with European nanomedicine societies. SFNano has more than 300 members.</p><p><b>S003</b></p><p><b>S003 Spanish Platform for Nanomedicine (NanoMed Spain)</b></p><p>Speaker: Dr. Teresa Sanchis</p><p><i>Spanish Platform for Nanomedicine (NanoMed Spain) – Institute for Bioengineering of Catalonia, Baldiri Reixach 1008028, Barcelona, (Spain)</i></p><p><b>Abstract</b>: The Spanish Platform for Nanomedicine (NanoMed Spain - http://nanomedspain.net/) is a forum that brings together 150 public research centres, hospitals, companies and government representatives active in nanomedicine. Nanomed Spain is an instrument to coordinate entities involved in R&amp;D+i, fundamental to the transfer of results to industry and the health system in this highly multidisciplinary field. It is also a means of connection to facilitate the internationalization of initiatives and projects, with the aim of improving the competitiveness of Spanish companies in this emerging field.</p><p>Industry in the biomedical and biotechnology sector plays a leading role in the Platform, very actively supported by technology centers, research organizations, universities and hospitals, as well as by national public administration.</p><p>The mission of Nanomed Spain is to promote and facilitate public-private partnerships in research and ","PeriodicalId":91547,"journal":{"name":"Journal of interdisciplinary nanomedicine","volume":"2 1","pages":"5-105"},"PeriodicalIF":0.0,"publicationDate":"2017-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1002/jin2.24","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47467657","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Towards a rational design of solid drug nanoparticles with optimised pharmacological properties","authors":"Marco Siccardi,&nbsp;Phillip Martin,&nbsp;Darren Smith,&nbsp;Paul Curley,&nbsp;Tom McDonald,&nbsp;Marco Giardiello,&nbsp;Neill Liptrott,&nbsp;Steve Rannard,&nbsp;Andrew Owen","doi":"10.1002/jin2.21","DOIUrl":"10.1002/jin2.21","url":null,"abstract":"<div>\u0000 \u0000 <p>Solid drug nanoparticles (SDNs) are a nanotechnology with favourable characteristics to enhance drug delivery and improve the treatment of several diseases, showing benefit for improved oral bioavailability and injectable long-acting medicines. The physicochemical properties and composition of nanoformulations can influence the absorption, distribution, and elimination of nanoparticles; consequently, the development of nanoparticles for drug delivery should consider the potential role of nanoparticle characteristics in the definition of pharmacokinetics. The aim of this study was to investigate the pharmacological behaviour of efavirenz SDNs and the identification of optimal nanoparticle properties and composition. Seventy-seven efavirenz SDNs were included in the analysis. Cellular accumulation was evaluated in HepG2 (hepatic) and Caco-2 (intestinal), CEM (lymphocyte), THP1 (monocyte), and A-THP1 (macrophage) cell lines. Apparent intestinal permeability (P_app) was measured using a monolayer of Caco-2 cells. The P_app values were used to evaluate the potential benefit on pharmacokinetics using a physiologically based pharmacokinetic model. The generated SDNs had an enhanced intestinal permeability and accumulation in different cell lines compared to the traditional formulation of efavirenz. Nanoparticle size and excipient choice influenced efavirenz apparent permeability and cellular accumulation, and this appeared to be cell line dependent. These findings represent a valuable platform for the design of SDNs, giving an empirical background for the selection of optimal nanoparticle characteristics and composition. Understanding how nanoparticle components and physicochemical properties influence pharmacological patterns will enable the rational design of SDNs with desirable pharmacokinetics.</p>\u0000 </div>","PeriodicalId":91547,"journal":{"name":"Journal of interdisciplinary nanomedicine","volume":"1 3","pages":"110-123"},"PeriodicalIF":0.0,"publicationDate":"2016-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1002/jin2.21","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"50843884","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Carbon screen-printed electrodes on ceramic substrates for label-free molecular detection of antibiotic resistance","authors":"Eleojo A. Obaje,&nbsp;Gerard Cummins,&nbsp;Holger Schulze,&nbsp;Salman Mahmood,&nbsp;Marc P.Y. Desmulliez,&nbsp;Till T. Bachmann","doi":"10.1002/jin2.16","DOIUrl":"10.1002/jin2.16","url":null,"abstract":"<div>\u0000 \u0000 <p>The growing threat posed by antimicrobial resistance on the healthcare and economic well-being of mankind is pushing the need to develop novel and improved diagnostic platforms for its rapid detection at point of care, facilitating better patient management strategies during antibiotic therapy. In this paper, we present the manufacturing and characterisation of a low-cost carbon screen-printed electrochemical sensor on a ceramic substrate. Using label-free electrochemical impedance spectroscopy, the sensor is demonstrated for the detection of <i>bla_NDM</i>, which is one of the main antimicrobial resistance factors in carbapenem-resistant Enterobacteriaceae. The electrochemical performance of the newly fabricated sensor was initially investigated in relation to the function of its underlying composite materials, evaluating the choice of carbon and dielectric pastes by characterising properties like surface roughness, wetting and susceptibility of unspecific DNA binding. Subsequently, the sensor was used in an electrochemical impedance spectroscopy assay for the sensitive and specific detection of synthetic <i>bla_NDM</i> targets achieving a detection limit of 200 nM. The sensor properties and performance demonstrated in this study proved the suitability of the new electrode materials and manufacturing for further point-of-care test development as an inexpensive and effective alternative to gold electrodes sensor.</p>\u0000 </div>","PeriodicalId":91547,"journal":{"name":"Journal of interdisciplinary nanomedicine","volume":"1 3","pages":"93-109"},"PeriodicalIF":0.0,"publicationDate":"2016-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1002/jin2.16","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"50843825","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}