Journal of interdisciplinary nanomedicine最新文献

{"title":"Abstract","authors":"","doi":"10.1002/jin2.24","DOIUrl":"10.1002/jin2.24","url":null,"abstract":"<p><b>S001</b></p><p><b>S001 The British Society for Nanomedicine</b></p><p>Speaker: Professor R. Steven Conlan</p><p><i>Swansea University Medical School, Singleton Park, Swansea, SA2 8PP, UK</i></p><p><b>Abstract</b>: With the global benefits of the new science of nanomedicine growing each year, the British Society for Nanomedicine enables open access for industry, academia, clinicians and the public to news and details of ongoing research throughout the UK. Our mission includes the direct explanation of the ongoing science and commercial developments to allow the public to understand and stay in touch with this exciting area as it impacts future global healthcare. The British Society for Nanomedicine runs the Journal of Interdisciplinary Nanomedicine (JOIN), an international peer-reviewed academic journal that aims to report truly interdisciplinary nanomedicine research. The British Society for Nanomedicine represents UK nanomedicine interests at a UK, EU and International level including the ETP nanomedicine, and European national nanomedicines platform. The British Society for Nanomedicine is proud to welcome you to ENM17 in London.</p><p><b>S002</b></p><p><b>S002 The French Society for Nanomedicine (SFNano)</b></p><p>Speaker: Dr Nathalie Mignet</p><p><i>University Paris Descartes, CNRS, Faculty of Pharmacy, Paris, France</i></p><p><b>Abstract</b>: The French Society for Nanomedicine (SFNano) is a non-lucrative association whose objectives are to favour progress and knowledge diffusion in the Nanomedicine domain. For these purposes, SFNano organises seminars, workshops and discussions in order to favour a french network, and broader, in collaboration with European nanomedicine societies. SFNano has more than 300 members.</p><p><b>S003</b></p><p><b>S003 Spanish Platform for Nanomedicine (NanoMed Spain)</b></p><p>Speaker: Dr. Teresa Sanchis</p><p><i>Spanish Platform for Nanomedicine (NanoMed Spain) – Institute for Bioengineering of Catalonia, Baldiri Reixach 1008028, Barcelona, (Spain)</i></p><p><b>Abstract</b>: The Spanish Platform for Nanomedicine (NanoMed Spain - http://nanomedspain.net/) is a forum that brings together 150 public research centres, hospitals, companies and government representatives active in nanomedicine. Nanomed Spain is an instrument to coordinate entities involved in R&amp;D+i, fundamental to the transfer of results to industry and the health system in this highly multidisciplinary field. It is also a means of connection to facilitate the internationalization of initiatives and projects, with the aim of improving the competitiveness of Spanish companies in this emerging field.</p><p>Industry in the biomedical and biotechnology sector plays a leading role in the Platform, very actively supported by technology centers, research organizations, universities and hospitals, as well as by national public administration.</p><p>The mission of Nanomed Spain is to promote and facilitate public-private partnerships in research and ","PeriodicalId":91547,"journal":{"name":"Journal of interdisciplinary nanomedicine","volume":"2 1","pages":"5-105"},"PeriodicalIF":0.0,"publicationDate":"2017-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1002/jin2.24","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47467657","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Towards a rational design of solid drug nanoparticles with optimised pharmacological properties","authors":"Marco Siccardi,&nbsp;Phillip Martin,&nbsp;Darren Smith,&nbsp;Paul Curley,&nbsp;Tom McDonald,&nbsp;Marco Giardiello,&nbsp;Neill Liptrott,&nbsp;Steve Rannard,&nbsp;Andrew Owen","doi":"10.1002/jin2.21","DOIUrl":"10.1002/jin2.21","url":null,"abstract":"<div>\u0000 \u0000 <p>Solid drug nanoparticles (SDNs) are a nanotechnology with favourable characteristics to enhance drug delivery and improve the treatment of several diseases, showing benefit for improved oral bioavailability and injectable long-acting medicines. The physicochemical properties and composition of nanoformulations can influence the absorption, distribution, and elimination of nanoparticles; consequently, the development of nanoparticles for drug delivery should consider the potential role of nanoparticle characteristics in the definition of pharmacokinetics. The aim of this study was to investigate the pharmacological behaviour of efavirenz SDNs and the identification of optimal nanoparticle properties and composition. Seventy-seven efavirenz SDNs were included in the analysis. Cellular accumulation was evaluated in HepG2 (hepatic) and Caco-2 (intestinal), CEM (lymphocyte), THP1 (monocyte), and A-THP1 (macrophage) cell lines. Apparent intestinal permeability (P_app) was measured using a monolayer of Caco-2 cells. The P_app values were used to evaluate the potential benefit on pharmacokinetics using a physiologically based pharmacokinetic model. The generated SDNs had an enhanced intestinal permeability and accumulation in different cell lines compared to the traditional formulation of efavirenz. Nanoparticle size and excipient choice influenced efavirenz apparent permeability and cellular accumulation, and this appeared to be cell line dependent. These findings represent a valuable platform for the design of SDNs, giving an empirical background for the selection of optimal nanoparticle characteristics and composition. Understanding how nanoparticle components and physicochemical properties influence pharmacological patterns will enable the rational design of SDNs with desirable pharmacokinetics.</p>\u0000 </div>","PeriodicalId":91547,"journal":{"name":"Journal of interdisciplinary nanomedicine","volume":"1 3","pages":"110-123"},"PeriodicalIF":0.0,"publicationDate":"2016-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1002/jin2.21","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"50843884","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Carbon screen-printed electrodes on ceramic substrates for label-free molecular detection of antibiotic resistance","authors":"Eleojo A. Obaje,&nbsp;Gerard Cummins,&nbsp;Holger Schulze,&nbsp;Salman Mahmood,&nbsp;Marc P.Y. Desmulliez,&nbsp;Till T. Bachmann","doi":"10.1002/jin2.16","DOIUrl":"10.1002/jin2.16","url":null,"abstract":"<div>\u0000 \u0000 <p>The growing threat posed by antimicrobial resistance on the healthcare and economic well-being of mankind is pushing the need to develop novel and improved diagnostic platforms for its rapid detection at point of care, facilitating better patient management strategies during antibiotic therapy. In this paper, we present the manufacturing and characterisation of a low-cost carbon screen-printed electrochemical sensor on a ceramic substrate. Using label-free electrochemical impedance spectroscopy, the sensor is demonstrated for the detection of <i>bla_NDM</i>, which is one of the main antimicrobial resistance factors in carbapenem-resistant Enterobacteriaceae. The electrochemical performance of the newly fabricated sensor was initially investigated in relation to the function of its underlying composite materials, evaluating the choice of carbon and dielectric pastes by characterising properties like surface roughness, wetting and susceptibility of unspecific DNA binding. Subsequently, the sensor was used in an electrochemical impedance spectroscopy assay for the sensitive and specific detection of synthetic <i>bla_NDM</i> targets achieving a detection limit of 200 nM. The sensor properties and performance demonstrated in this study proved the suitability of the new electrode materials and manufacturing for further point-of-care test development as an inexpensive and effective alternative to gold electrodes sensor.</p>\u0000 </div>","PeriodicalId":91547,"journal":{"name":"Journal of interdisciplinary nanomedicine","volume":"1 3","pages":"93-109"},"PeriodicalIF":0.0,"publicationDate":"2016-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1002/jin2.16","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"50843825","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Abstract","authors":"","doi":"10.1002/jin2.14","DOIUrl":"10.1002/jin2.14","url":null,"abstract":"<p>Shahd Abuhelal (<span>[email protected]</span>)*</p><p><i>King's College London/Institute of Pharmaceutical Science</i></p><p>siRNA treatment can result in decreased protein expression and could be used to treat cancer or other diseases. Successful application depends on efficient delivery inside target cells. Here, we aim to design and optimise a nano-carrier suitable for siRNA delivery, with high encapsulation efficiency and stability for use in vitro and in vivo to target cancer.</p><p>Liposomes and pH-sensitive peptides assembled as ternary complex were investigated as siRNA delivery systems. Physicochemical characteristics (size, zeta potential, siRNA maintenance, release and aggregation) were tested. Cell uptake and luciferase knock down were evaluated in vitro, and some complexes tested for biodistribution <i>in vivo</i>.</p><p>Hydrodynamic size and zeta potential of the lipoplex, peptide complexes and ternary complexs were similar. Although lipoplexes showed better encapsulation, they were less stable in serum. Ternary complexes offered better protection for the siRNA. Improved cell uptake was seen for ternary complexes in comparison with peptide complex and lipoplex. Knock down studies revealed optimal effects ternary complexes, and preliminary <i>in vivo</i> experiments showed tumour accumulation of lipoplex.</p><p>These siRNA delivery vehicles appear promising for in vivo applications, and work is now focused on the improvement of cell targeting, in vitro and in vivo PK/PD.</p><p>Chris Adams (<span>[email protected]</span>)*</p><p><i>Keele University</i></p><p>Magnetic nanoparticles (MNPs) are key translational platforms with the ability to label cells for non-invasive imaging and genetically engineer cells for release of therapeutic biomolecules. We show for the first time that application of magnetic fields can safely enhance MNP mediated labelling and genetic engineering of autologous canine olfactory mucosal cells (cOMCs), a key veterinary cell population for treatment of spinal injury in companion dogs. Crucially, the developed protocols were successfully combined with advanced minicircle DNA vectors to deliver brain derived neurotrophic factor (important in promoting nerve fibre outgrowth) to cOMCs. Minicircles have distinct advantages for clinical gene delivery due to their small size, lack of bacterial backbone and duration of transgene expression. Finally, we also show that MNP labelling can facilitate imaging of cOMCs encapsulated in implantable collagen hydrogels using non-invasive magnetic resonance imaging. A combination of these methodologies could enable translation of safe and effective cOMC transplantation strategies.</p><p>Mohammad Ahmad Abdallah Al-Natour (<span>[email protected]</span>)*</p><p><i>University and Institution: University of Nottingham</i></p><p><b>Abstract</b></p><p>Recent years have witnessed unexpected growth of research on the medical applications of nanotechnology (nanomedicine), especially the ","PeriodicalId":91547,"journal":{"name":"Journal of interdisciplinary nanomedicine","volume":"1 2","pages":"35-82"},"PeriodicalIF":0.0,"publicationDate":"2016-08-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1002/jin2.14","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"50843753","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Encapsulation of the p38 MAPK inhibitor GSK 678361A in nanoparticles for inflammatory-based disease states","authors":"Baljinder K Bains,&nbsp;Michelle K Greene,&nbsp;Leona M McGirr,&nbsp;Jay Dorman,&nbsp;Stuart N Farrow,&nbsp;Christopher J Scott","doi":"10.1002/jin2.9","DOIUrl":"10.1002/jin2.9","url":null,"abstract":"<div>\u0000 \u0000 <p>Inhibitors of p38 mitogen-activated protein kinase (MAPK) are currently being pursued as therapeutics in inflammatory conditions, but many candidates have demonstrated limited efficacy or toxicity issues to date. Nanoformulation of p38 MAPK inhibitors may overcome these challenges, by enabling controlled release and targeted delivery. Thus, the aim of this study was to develop a nanoformulation of the p38 MAPK inhibitor GSK 678361A and subsequently validate its anti-inflammatory efficacy in vitro, versus the drug in its free format. Poly(lactic-co-glycolic acid) nanoparticles encapsulating GSK 678361A were prepared via a salting-out method and characterised by photon correlation spectroscopy, scanning electron microscopy and high-performance liquid chromatography. The anti-inflammatory effect of both free and nanoformulated GSK 678361A was evaluated in cultures of lipopolysaccharide-stimulated macrophages, with subsequent enzyme-linked immunosorbent assay analysis of TNF-α and IL-6 providing readouts of efficacy. A controlled release nanoformulation of GSK 678361A was successfully developed, with physicochemical characterisation revealing an average particle diameter of 115.5 ± 3.5 nm and polydispersity index of 0.13 ± 0.03, indicative of a homogeneous size distribution. GSK 678361A loading was quantified at 10.1 ± 0.4 µg per mg of poly(lactic-co-glycolic acid), equating to an entrapment efficiency of approximately 50%. When tested in cultures of lipopolysaccharide-stimulated macrophages, GSK 678361A nanoparticles inhibited the production of pro-inflammatory cytokines to an extent that was largely comparable with the free drug, although superior efficacy of the nanoformulation was observed at selected doses. These studies indicate that GSK 678361A may be successfully nanoformulated without loss of drug activity, warranting further evaluation in models of inflammation in vivo. © 2016 The Authors. Journal of Interdisciplinary Nanomedicine published by John Wiley &amp; Sons Ltd and the British Society for Nanomedicine</p>\u0000 </div>","PeriodicalId":91547,"journal":{"name":"Journal of interdisciplinary nanomedicine","volume":"1 3","pages":"85-92"},"PeriodicalIF":0.0,"publicationDate":"2016-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1002/jin2.9","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"50843539","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Preparation, loading, and cytotoxicity analysis of polymer nanotubes from an ethylene glycol dimethacrylate homopolymer in comparison to multi-walled carbon nanotubes","authors":"Ben Newland,&nbsp;Laurent Thomas,&nbsp;Yu Zheng,&nbsp;Martin Steinhart,&nbsp;Carsten Werner,&nbsp;Wenxin Wang","doi":"10.1002/jin2.7","DOIUrl":"10.1002/jin2.7","url":null,"abstract":"<div>\u0000 \u0000 <p>Despite concerns over toxicity, carbon nanotubes have been extensively investigated for potential applications in nanomedicine because of their small size, unique properties, and ability to carry cargo such as small molecules and nucleic acids. Herein, we show that polymer nanotubes can be synthesized quickly and easily from a homopolymer of ethylene glycol dimethacrylate (EGDMA). The nanotubes formed via photo-initiated polymerization of the highly functional prepolymer, inside an anodized aluminium oxide template, have a regular structure and large internal pore and can be loaded with a fluorescent dye within minutes representing a simple alternative to multi-walled carbon nanotubes for biomedical applications.</p>\u0000 </div>","PeriodicalId":91547,"journal":{"name":"Journal of interdisciplinary nanomedicine","volume":"1 1","pages":"9-18"},"PeriodicalIF":0.0,"publicationDate":"2016-04-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1002/jin2.7","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34641915","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}