Journal of microbiology & experimentation最新文献_第4页

Application of real-time (RT-PCR) for detection of Salmonella Typhi among febrile patients in Khartoum state 实时（RT-PCR）在喀土穆州发热患者伤寒沙门氏菌检测中的应用

Journal of microbiology & experimentation Pub Date : 2020-10-28 DOI: 10.15406/jmen.2020.08.00305

Alsayid A Aldusogi, K. Enan

{"title":"Application of real-time (RT-PCR) for detection of Salmonella Typhi among febrile patients in Khartoum state","authors":"Alsayid A Aldusogi, K. Enan","doi":"10.15406/jmen.2020.08.00305","DOIUrl":"https://doi.org/10.15406/jmen.2020.08.00305","url":null,"abstract":"Background: Typhoid fever, caused by Salmonella enteric sero-var Typhi remains a public health threat in many countries particularly those with poor sanitary conditions. Ambulatory health care facilities in endemic settings frequently lack laboratory-based diagnostics, resulting in the majority of diagnosis being made clinically and antimicrobials given empirically so we need more developed and specific methods. To detect the causative agents. The objective of this study was to apply Real-time (RT-PCR) for detection of salmonella Typhi among febrile patients at Khartoum state-Sudan. Methods: Blood samples were taken from 100 suspected typhoid cases, they were subjected to conventional blood culture; widal agglutination test and real-time PCR. Blood culture was performed using standard protocol and real time PCR targeting prg K gene. Result: Out of 100 suspected typhoid cases blood culture were positive in 24 cases. The Real-time assay identified 20cases (83%) as positives among the 24 culture Positive cases. However, the assay additionally detected 20 (26%) of cases as Salmonella infection among culture negative patients. Widal test was positive in 16(66.6%). Cases among culture positive cases. However, the test additionally was positive in 44(57.8%) cases among culture negative cases. Conclusions: Our study conclude that PCR Real-time is a rapid, sensitive, and specific test for the diagnosis of typhoid fever especially during antibiotic treatment and/or cultured one in late stages of disease. of Sybr-Green added of specimen DNA. Pure culture DNA Salmonella typhi as positive and Escherichia coli, Acinetobacter baumanni, and pneumoniae used as negative controls.","PeriodicalId":91326,"journal":{"name":"Journal of microbiology & experimentation","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49545330","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Cutaneous leishmaniasis mimicking sarcoidosis in Libyan patient: A case report 利比亚患者皮肤利什曼病样结节病一例报告

Journal of microbiology & experimentation Pub Date : 2020-10-12 DOI: 10.15406/jmen.2020.08.00304

A. Amro, Hamida Al-Dwibe, Ali Lashhab, Esseid Elzubi, Walid K. Saadawi, Aisha Gashout

{"title":"Cutaneous leishmaniasis mimicking sarcoidosis in Libyan patient: A case report","authors":"A. Amro, Hamida Al-Dwibe, Ali Lashhab, Esseid Elzubi, Walid K. Saadawi, Aisha Gashout","doi":"10.15406/jmen.2020.08.00304","DOIUrl":"https://doi.org/10.15406/jmen.2020.08.00304","url":null,"abstract":"Leishmaniasis is vector–borne disease caused by parasitic protozoans belonging to the genus Leishmania and is transmitted by infected phlebotomine sand flies . Sarcoidosis is a multisystemic disorder of unknown cause characterized by the formation of immune granulomas in affected organs. Clinical symptoms, severity and evolution of sarcoidosis are highly heterogeneous and can lead to other diseases with similar clinical and pathologic presentations. In this report we present a case of 77 years-old diabetic Libyan male, with chronic erythematous indurated plaques, and nodules on the face. The patients were treated by multiple physicians with topical and systemic corticosteroids for 25 years without improvement. Microscopic examination of Giemsa stained smears from all lesions showed numerous Leishmania amastigotes in and outside monocytes. Leishmania tropica was identified as causative species. The patient was treated with combination of oral rifampicin (600 mg/day) and isoniazide (300 mg/day) and followed up for 9 months until skin-slit smears and PCR turned negative. In conclusion, CL can be misdiagnosed clinically with any granulomatous skin lesions which are compatible with sarcoidal type granuloma. Molecular diagnosis of CL by implementing Leishmania -specific PCR approaches should be performed routinely in any granulomatous skin lesion.","PeriodicalId":91326,"journal":{"name":"Journal of microbiology & experimentation","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-10-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45426270","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Prevalence of schistosomiasis among school aged children in Altakamol area, Khartoum state, Sudan 苏丹喀土穆州Altakamol地区学龄儿童血吸虫病患病率

Journal of microbiology & experimentation Pub Date : 2020-10-02 DOI: 10.15406/jmen.2020.08.00303

M. Hamad, Trig Mohamed Elfaki, Essam Zarrug, Hussein Omer Musa Mohammed, Sally Hassan Mohammed, Randa Alaageb Haj Ahmad, Rabab Mohammed Eltaher, Tasneem Isamaldein Ahamed Karrar

{"title":"Prevalence of schistosomiasis among school aged children in Altakamol area, Khartoum state, Sudan","authors":"M. Hamad, Trig Mohamed Elfaki, Essam Zarrug, Hussein Omer Musa Mohammed, Sally Hassan Mohammed, Randa Alaageb Haj Ahmad, Rabab Mohammed Eltaher, Tasneem Isamaldein Ahamed Karrar","doi":"10.15406/jmen.2020.08.00303","DOIUrl":"https://doi.org/10.15406/jmen.2020.08.00303","url":null,"abstract":"Schistosoma haematobium is a causative agent of urinary Schistosomiasis and Schistosoma mansoni causing intestinal Schistosomiasis which is wide spread worms in the world and are considered a foremost health problem in the Sudan. Cross sectional study was carried out among school aged children in Altakamol area - Khartoum state, from February to March 2017 to recognize the prevalence of Schistosoma haematobium and Schistosoma mansoni among school children in the area. One hundred sixty samples were collected randomly using random systematic manner including, 80samples from male and 80 from female. Forty urine samples and 40 stool samples were collected form student at class 3, 5, 6 and 7 and examined macroscopically and microscopically. The data collected through questionnaire. Of the 160 participants6 infected with Schistosoma hematobium and 2 were infected with Schistosoma mansoni. The overall prevalence of Schistosomiasis in the area was 5%.Significant association was observed between gender and age and acquiring Schistosoma infection (p =0.01). Further study among school children with large sample size is needed.","PeriodicalId":91326,"journal":{"name":"Journal of microbiology & experimentation","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42208803","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Synthesis and antibacterial activity of 3-nitrobenzaldehyde semicarbazone ligand and its Ni (II) and Cu (II) Complexes 3-硝基苯甲醛缩氨基脲配体及其Ni（II）和Cu（II）配合物的合成及其抗菌活性

Journal of microbiology & experimentation Pub Date : 2020-09-28 DOI: 10.15406/JMEN.2020.08.00302

M. Hamad, M. B. Hussein

引用次数: 5

Investigation of Hepatitis A virus outbreak in Aligarh and its peripheral areas, Uttar Pradesh, India 印度北方邦阿里加尔及其周边地区甲型肝炎病毒爆发调查

Journal of microbiology & experimentation Pub Date : 2020-08-31 DOI: 10.15406/jmen.2020.08.00301

Islam Ahmad, Hiba Sami, Z. Mustafa, Ghazanfar Ali, I. Shukla, A. Raza, H. Khan

引用次数: 1

War against antimicrobial resistance 对抗抗菌素耐药性的战争

Journal of microbiology & experimentation Pub Date : 2020-08-31 DOI: 10.15406/jmen.2020.08.00300

S. T. Akinwotu, Oluwaseun Fapohunda

{"title":"War against antimicrobial resistance","authors":"S. T. Akinwotu, Oluwaseun Fapohunda","doi":"10.15406/jmen.2020.08.00300","DOIUrl":"https://doi.org/10.15406/jmen.2020.08.00300","url":null,"abstract":"Resistance to antimicrobials is a global threat that demands immediate response. The excessive use and misuse of antimicrobials has led to the development of multidrug resistant strains of microorganisms. As the consumption rate of these drugs increases, the resistance rate also increases, resulting in high cost of medical treatment and increased mortality rate. To war against antimicrobial resistance, the biochemical and genetic mechanism of resistance in microorganisms is a key factor to be considered. Recent articles from reputable journals/archives including PubMed, Scopus, Google Scholar, ResearchGate etc were retrieved and studied and we discovered that much have been researched on the importance of drugs in the treatment of antimicrobial resistance and in recent decade researchers have been delving into important botanicals that could address multidrug resistance with several opinions. Researchers have opinionated that phytoconstituents could be potential drugs that would address antimicrobial resistance with little or no side effect and they are relatively cheap and readily available to low-income people. However, the future is largely going to be machine driven and little have been reported on the emerging technologies that could adequately tackle this menace of antimicrobial resistance. Here we succinctly discussed how some of these promising technologies could be employed in combating the resistance of microorganism to antibiotics; the use of vaccine technologies, the use of Artificial Intelligence coupled with machine learning algorithms, the use of nanosystems and the use of CRISPR-cas technology.","PeriodicalId":91326,"journal":{"name":"Journal of microbiology & experimentation","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47817155","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Numerical and statistical evaluation of nitric oxide effect on leaf anatomy of Triticum genotypes under salinity stress 盐度胁迫下一氧化氮对小麦基因型叶片解剖结构影响的数值与统计评价

Journal of microbiology & experimentation Pub Date : 2020-08-19 DOI: 10.15406/jmen.2020.08.00299

Bahattin Bozdağ, A. Özdemir, Mehmet Hamurcu, C. Özdemir, E. Hakki, S. Gezgin

引用次数: 0

The low cases reported in Sudan regarding a pandemic COVID-19 and Sudan’s Health system responding 苏丹关于COVID-19大流行和苏丹卫生系统应对的低病例报告

Journal of microbiology & experimentation Pub Date : 2020-07-20 DOI: 10.15406/JMEN.2020.08.00298

Abdelhakam G. Tamomh, B. Almugadam, A. Elkhalifa

引用次数: 0

Detection of aflatoxins in smoked fish (Clarias anguillaris) sold in the Bamako District 巴马科区出售的熏鱼中黄曲霉毒素的检测

Journal of microbiology & experimentation Pub Date : 2020-07-13 DOI: 10.15406/JMEN.2020.08.00297

O. Diarra, Christiane Demble, A. Babana, F. Samaké, Boubacar Madio dit Aladiogo Maiga, K. Maiga, M. Diarra, A. Traoré, D. Ouattara, Djeneba Nantoumé, Ibrahim Mallé

{"title":"Detection of aflatoxins in smoked fish (Clarias anguillaris) sold in the Bamako District","authors":"O. Diarra, Christiane Demble, A. Babana, F. Samaké, Boubacar Madio dit Aladiogo Maiga, K. Maiga, M. Diarra, A. Traoré, D. Ouattara, Djeneba Nantoumé, Ibrahim Mallé","doi":"10.15406/JMEN.2020.08.00297","DOIUrl":"https://doi.org/10.15406/JMEN.2020.08.00297","url":null,"abstract":"Summary Fishing is a millennial tradition in Mali. It is an important sub-sector of the Malian economy, depending in particular on the Niger River, which is 4,200 km long, including 1,700 km in Mali. Its potential importance of existing resources is expressed in its socio-cultural role, job creation, food security and poverty reduction. Due to their pervasiveness, mycotoxin-producing molds are able to reduce the nutritional value of smoked fish by developing several mycotoxins. Smoked fish contaminated with mycotoxins have harmful effects on human health. Samples of smoked fish for sale were collected in the six communes of the district of Bamako and analyzed to determine the fungal flora as well as the concentration of mycotoxins. The mycoflora study of smoked fish has shown that the concentration of fungi varies between 0.5.10 4 CFU/g to 14.10 4 CFU/g DM. whereas aflatoxins by the ELISA method aflatoxin kits (AF) (RIDASCREEN FAST, R-Biopharm AG) were detected in 60 samples taken. This study indicates the need for a continuous assessment of the mycological state of the smoked fish production chain in order to guarantee consumer safety. SAS software was used for all analyzes and the differences were considered significant when p<0.05.","PeriodicalId":91326,"journal":{"name":"Journal of microbiology & experimentation","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-07-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47894111","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Prevalence of human immunodeficiency virus among tuberculosis patients in Khartoum state 喀土穆州肺结核患者中人类免疫缺陷病毒的流行情况

Journal of microbiology & experimentation Pub Date : 2020-06-30 DOI: 10.15406/JMEN.2020.08.00296

M. Hamad, Waseem Sameer Kwami, Abass A. Ahmed

{"title":"Prevalence of human immunodeficiency virus among tuberculosis patients in Khartoum state","authors":"M. Hamad, Waseem Sameer Kwami, Abass A. Ahmed","doi":"10.15406/JMEN.2020.08.00296","DOIUrl":"https://doi.org/10.15406/JMEN.2020.08.00296","url":null,"abstract":"Introduction: Pulmonary Tuberculosis (TB) and human immunodeficiency virus (HIV) have been closely connected since the appearance of acquired immunodeficiency syndrome (AIDS). HIV infection among active TB cases provides real challenges in diagnosis and treatment of TB. This is descriptive cross-sectional study conducted at Abu-anja Teaching Hospital; Khartoum state in period from October 2018to March 2019.The objective was to determine the prevalence of Human Immunodeficiency Virus infection among tuberculosis patients. Method: A total of 90 venous blood samples were collected from the study participants, serum was obtained by centrifugation at (3000 rpm) for 5 minutes. The levels of HIV antibody titer were determined by using sandwich ELISA Assay. Results: The studies revealed that out of the 90 participants of the tuberculosis patients, 5(5.6%) were HIV positive, and the HIV prevalence among male patients were significantly higher than female patients. Conclusion: The study concluded that there is high prevalence of HIV among tuberculosis patients in Khartoum state, capital of Sudan (5.6%) comparing with (5%) at which the WHO recommended intensified intervention to address TB-HIV co-infection critical in any strategy that aims to reach those most in need.","PeriodicalId":91326,"journal":{"name":"Journal of microbiology & experimentation","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48187498","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1