Detection of aflatoxins in smoked fish (Clarias anguillaris) sold in the Bamako District

O. Diarra, Christiane Demble, A. Babana, F. Samaké, Boubacar Madio dit Aladiogo Maiga, K. Maiga, M. Diarra, A. Traoré, D. Ouattara, Djeneba Nantoumé, Ibrahim Mallé
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Abstract

Summary Fishing is a millennial tradition in Mali. It is an important sub-sector of the Malian economy, depending in particular on the Niger River, which is 4,200 km long, including 1,700 km in Mali. Its potential importance of existing resources is expressed in its socio-cultural role, job creation, food security and poverty reduction. Due to their pervasiveness, mycotoxin-producing molds are able to reduce the nutritional value of smoked fish by developing several mycotoxins. Smoked fish contaminated with mycotoxins have harmful effects on human health. Samples of smoked fish for sale were collected in the six communes of the district of Bamako and analyzed to determine the fungal flora as well as the concentration of mycotoxins. The mycoflora study of smoked fish has shown that the concentration of fungi varies between 0.5.10 4 CFU/g to 14.10 4 CFU/g DM. whereas aflatoxins by the ELISA method aflatoxin kits (AF) (RIDASCREEN FAST, R-Biopharm AG) were detected in 60 samples taken. This study indicates the need for a continuous assessment of the mycological state of the smoked fish production chain in order to guarantee consumer safety. SAS software was used for all analyzes and the differences were considered significant when p<0.05.
巴马科区出售的熏鱼中黄曲霉毒素的检测
捕鱼是马里千年传统。它是马里经济的一个重要子部门,尤其依赖于长达4200公里的尼日尔河,其中包括马里的1700公里。其现有资源的潜在重要性体现在其社会文化作用、创造就业、粮食安全和减贫方面。由于其普遍性,产生真菌毒素的霉菌能够通过产生几种真菌毒素来降低熏鱼的营养价值。被真菌毒素污染的熏鱼对人类健康有有害影响。在巴马科区的六个社区收集了出售的熏鱼样本,并对其进行了分析,以确定真菌区系以及真菌毒素的浓度。对熏鱼的菌群研究表明,真菌的浓度在0.5.10 4 CFU/g至14.10 4 CFU/gDM之间。而通过ELISA方法,黄曲霉毒素试剂盒(AF)(RIDASCREEN FAST,R-Biopharm AG)在60个样本中检测到黄曲霉毒素。这项研究表明,需要对熏鱼生产链的真菌状态进行持续评估,以确保消费者安全。所有分析均使用SAS软件,当p<0.05时,差异被认为是显著的。
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