{"title":"Physico-Chemical Characterization and Assessment of Cytotoxic and Genotoxic Effects of Poly-Ethylene-Glycol Coated and Uncoated Gold Nanoparticles on Human Kidney (HK-2) Cells","authors":"Rogers Cr, D. S, Patlolla Ak, Tchounwou Pb","doi":"10.26420/austinjenvirontoxicol.2021.1042","DOIUrl":"https://doi.org/10.26420/austinjenvirontoxicol.2021.1042","url":null,"abstract":"Although gold Nanoparticles (Au-NPs) have been widely used in medicine for the diagnosis and treatment of patients due to their unique physicochemical properties, chemical stability and biocompatibility, recent reports have also highlighted their potential to induce toxicity to humans. In the present study, we investigated the toxic effects of uncoated and Polyethylene Glycol (PEG)-coated AuNPs on human kidney (HK-2) cells. Both forms of AuNP were synthesized and characterized using standard protocols. Dynamic Light Scattering (DLS), Zeta Sizer Nano ZS analyzer, Transmission Electron Microscopy (TEM), and Inductively Coupled Plasma-Optical Emission Spectroscopy (ICP-OES) were used to measure their distribution, zeta potential/surface charge, morphological size, and Au concentrations, respectively. Cytotoxicity was measured by Cyto- Tox assay and trypan blue exclusion test. Oxidative Stress (OS) was assessed by quantifying the levels of Glutathione (GSH), and Mitochondria Membrane Potential (MMP). Genotoxicity was assessed by single cell gel electrophoresis (Comet assay) and Chromosomal Aberration (CA) assay. Uncoated AuNPs significantly reduced cell viability, increased ROS, decreased GSH, depolarized the MMP, and induced significant DNA damage and chromosomal alterations including chromosome gaps, centric rings, breaks, deletions, and intra and interchromosome exchanges, in a concentration-dependent manner. PEG-coated AuNPs displayed lower cytotoxic and genotoxic effects, and did not produce any significant increase in ROS or significant decrease in GSH along with negligible polarization of the MMP. Hence, PEG-coated AuNPs are relatively less toxic than uncoated AuNPs and therefore, may have potential applications in nanomedicine.","PeriodicalId":91210,"journal":{"name":"Austin journal of environmental toxicology","volume":"10 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-12-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89518267","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Detection on Electroplating Effluent-Induced Cytopathological Alterations and DNA Damage in the Blood Samples of Cirrhinus mrigala (Hamilton, 1822)","authors":"S. R., B. S, R. S., M. M, M. R, Selvaraj J","doi":"10.26420/austinjenvirontoxicol.2021.1041","DOIUrl":"https://doi.org/10.26420/austinjenvirontoxicol.2021.1041","url":null,"abstract":"In the present study, fresh waterfish Cirrhinus mrigala has been used as a model organism to understand the mechanistic link between electroplating industrial effluent (EIE) and oxidative stress in aquatic ecosystems. To this end, the fishes were exposed to industrial effluent, in the tank water for 10, 20 and 30 days under controlled laboratory conditions. Various hematological parameters (Total RBC and WBC, Hemoglobin, MCV, MCH, PCV), biochemical metabolites (Glucose, protein, Cholesterol) and markers of genotoxicity (comet assay and micronuclear assay) were assessed. Fishes exposed for 30 days showed marked increase (p <0.001) in RBC with concomitant decrease in total WBC. There is significant (p <0.001) decline in indices of blood cells such as Hb, MCV, MCH and PCV. Results from comet assay showed significantly (p < 0.05) increased frequency genotoxicity in erythrocytes of as evident from observed increase in tail length in fishes exposed to EIE for 30 days. Overall, the results of this study, clearly demonstrated concentration dependent response of electroplating industrial effluent on freshwater fish Cirrhinus mrigala on haematological, biochemical and genotoxic markers. The results obtained herein suggests EIE as potential xenobiotics contributing towards ecotoxic and genotoxic effect in fishes.","PeriodicalId":91210,"journal":{"name":"Austin journal of environmental toxicology","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-10-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90198259","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Malashetty Vb, Biradar Pm, D. R, Hegde Ss, Nagabhushan C
{"title":"Reproductive and Developmental Toxicity of Triclosan (TCN) in Wistar Rats","authors":"Malashetty Vb, Biradar Pm, D. R, Hegde Ss, Nagabhushan C","doi":"10.26420/austinjenvirontoxicol.2021.1040","DOIUrl":"https://doi.org/10.26420/austinjenvirontoxicol.2021.1040","url":null,"abstract":"Triclosan (TCN) was evaluated for reproductive and developmental toxicity on male and female reproductive performance such as gonadal function, mating behaviour, conception, development of the conceptus and parturition. Wistar rats (10/sex/dose) were administered by oral gavage at the dose levels of 0, 25, 75 and 150 mg/kg bwt/day, prior to mating, during mating and post-mating periods (for males), during pregnancy and up to lactation day 13 (for females). The results showed that, no treatment related mortality or clinical signs. Body weight, food consumption, pre-coital time, gestation length, mating and fertility parameters, percentage of pre- and post-implantation losses, anogenital distance, anogenital ratio were not affected by the treatment. The male pups did not exhibit areola/nipple retention on postnatal day 13. Treatment resulted in significantly lower mean litter size, mean viable litter size and mean number of implantations at 150mg/kg bwt/day. The Thyroid Stimulating Hormone (TSH) and Thyroxine (T4) levels in adult rats and pups were not affected. External evaluation of pups as well as gross and microscopic examination of the reproductive organs of the parent animals revealed no adverse triclosan related changes. Considering the changes observed in the mean litter size, mean viable litter size, mean number of implantations and Day 4 surviva index at 150mg/ kg bwt/day, the No Observed Adverse Effect Level (NOAEL)” for reproductive toxicity is considered to be 75mg/kg Bwt/day.","PeriodicalId":91210,"journal":{"name":"Austin journal of environmental toxicology","volume":"50 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-09-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86456014","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Deposition Rates of Asthma Triggers on Conventional and Enclosed Window Treatments","authors":"Thorner Ps, Metwali N, W. Ng","doi":"10.26420/austinjenvirontoxicol.2021.1039","DOIUrl":"https://doi.org/10.26420/austinjenvirontoxicol.2021.1039","url":null,"abstract":"Environmental interventions are an important element of managing allergies and asthma. Health professionals often recommend that draperies be replaced with window blinds however no data exist on accumulation of inhalant allergens or inflammatory bioaerosols on window treatments. Installing blinds that accumulate less dust may reduce breathing zone exposures when blinds are adjusted if hazardous amounts of bioaerosols are deposited. We sought to determine the rate of accumulation of dust, allergens, bacterial endotoxin and fungal glucan on window blinds of two distinct types mounted on the two types of windows most commonly installed in U.S. homes. The blinds tested were conventional horizontal slat blinds hanging on the inside of the window (roomside blinds) and similar blinds placed between the exterior window glass and an extra pane of glass on the interior side (between-glass blinds). The study was conducted in six households as a paired, repeated measures study. Households were identified for participation, having met the study criteria of children and cats living inside a carpeted home. Standard window blinds accumulated cat allergen, endotoxin and fungal glucan at rates of 5940ng/m², 1910EU/m², and 11,360ng/m² per month. Between-glass blinds reduced the loading of asthma triggers by 25- to 185-fold. Comparison with clinical thresholds associated with asthma morbidity indicates that room-side blinds accumulate potentially hazardous quantities of asthma triggers.","PeriodicalId":91210,"journal":{"name":"Austin journal of environmental toxicology","volume":"11 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77038937","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Hosseini Sa, M. Akhgari, H. Hassanian-Moghaddam, H. Ss, F. Shahbazi, H. NazariKangavari, Akhavan Tavakoli Ar, M. Rafizadeh, A. Rafizadeh, Safety Promotion
{"title":"Screening of Suspected Liquids to Alcoholic Drinks by a Qualitative Chemical Kit","authors":"Hosseini Sa, M. Akhgari, H. Hassanian-Moghaddam, H. Ss, F. Shahbazi, H. NazariKangavari, Akhavan Tavakoli Ar, M. Rafizadeh, A. Rafizadeh, Safety Promotion","doi":"10.26420/austinjenvirontoxicol.2021.1038","DOIUrl":"https://doi.org/10.26420/austinjenvirontoxicol.2021.1038","url":null,"abstract":"Alcohol consumption is prohibited in Iran and it has caused many problems in various fields. This research is done to evaluate a new qualitative screening kit (for rapid detection of alcoholic beverages from nonalcoholic liquids) and to avoid more wasting time and money. For this purpose, the ethanol content of 839 suspected liquids prepared from the alcohol lab of legal medicine (Guilan province, Iran) was measured by a gas chromatography apparatus. At the same time, a newly designed kit based on the sulfochromic acid method was also used to separate negative samples (with ethanol equal or less than 3% volume/volume) from the others. Out of 839 samples, the ethanol content of 163 (19.43%) cases was appraised negatively. While most of the samples (676 cases or 80.57%) had ethanol more than 3% (positive results) with a mean of 21.68mg/L. Three samples (0.36%) had high levels of methanol which were interpreted as false positive due to the nonspecificity of the used method. Our results suggest the used kit has suitable efficiency for screening ethanol content of suspected liquids because the negative results of the kit have definite diagnostic value. However, a confirmative test (like gas chromatography) is needed for validation of the positive results.","PeriodicalId":91210,"journal":{"name":"Austin journal of environmental toxicology","volume":"22 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73810190","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Divya Kr, Midhun Tr, Moushmi Ks, Cheriyan As, Nisari Ar, Ratheeshkumar Cs, P. Shaiju, Martin Gd, N. Chandramohanakumar
{"title":"Stability and Presence of Pesticide Residue Sample Extracts of Soil and Vegetable: Eloor & Edayar Region, Kerala, Industrial Hub Nearer to Arabian Sea","authors":"Divya Kr, Midhun Tr, Moushmi Ks, Cheriyan As, Nisari Ar, Ratheeshkumar Cs, P. Shaiju, Martin Gd, N. Chandramohanakumar","doi":"10.26420/AUSTINJENVIRONTOXICOL.2021.1036","DOIUrl":"https://doi.org/10.26420/AUSTINJENVIRONTOXICOL.2021.1036","url":null,"abstract":"Eloor-Edayar, (latitude 9° 3´N and 10° 6´N and longitudes 76° 18´E and 76° 30´ and an area of 14.21km2) the landmass situated on the banks of River Periyar, is known as the industrial backbone of Kerala. The previous reports reveal this region is highly contaminated by heavy metal from industrial effluents. The present study focuses on pesticides in vegetables (Ladies finger, Papaya, Kachil- yam, Guava, Tapioca, Ginger) and related soil extracts when kept in cold conditions and their presence after a long period. The samples collected in May 2008 pre-monsoon, the environment when the soil and vegetables were free from volatile organic components, accelerates the adsorption of pesticides and high temperature. The samples shoot vegetable ladies finger, edible root vegetables Kachil-Yum, Tapioca and Ginger, fruits like Papaya and Guava and the sediments nearer land. The sample extracts analyzed in 2019 after keeping in the cold as well as the usual conditions. The Analysis report shows the pesticides present in the vegetables and soil are a, β, γ BHC Aldrin, Dicofol, a, β, Endosulfan, Dieldrin, OPDDT and PPDDT. Aldrin, Dicofol and BHC are the related forms in ladies Finger contains γ BHC, a, β, Endosulfan, Dieldrin, Aldrin, PPDDT. Ginger added a, β, γ BHC Aldrin, Dicofol, a, β, Endosulfan, OPDDT and PPDDT. Tapioca carried a, β, γ BHC, Dicofol and PPDDT. Kachil-Yum absorbed a, β, γ BHC Aldrin, Dicofol, a, β, Endosulfan, OPDDT and PPDDT. Papaya contains a and γ BHC. Guava captivated a, γ BHC Aldrin, Dicofol, a Dieldrin OPDDT and PPDDT.","PeriodicalId":91210,"journal":{"name":"Austin journal of environmental toxicology","volume":"18 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-03-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84813947","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
G. Akshatha., M. Abhilash, K. Jagadish, Shivanna Srikantaswamy
{"title":"Hybrid Nano-Composites by Deposition of Ag2WO4/ Carbon Nanotube: Efficient Biological Application","authors":"G. Akshatha., M. Abhilash, K. Jagadish, Shivanna Srikantaswamy","doi":"10.26420/AUSTINJENVIRONTOXICOL.2021.1035","DOIUrl":"https://doi.org/10.26420/AUSTINJENVIRONTOXICOL.2021.1035","url":null,"abstract":"For nearly a decade, researchers have debated pollution prevention to achieve a sustainable environment Recently, various nanoscale materials, including silver tungstate (Ag2WO4) nanoparticles, have been actively studied for their capacity to effectively prevent bacterial growth. A critical challenge is to enhance the antibacterial properties of nanomaterials while maintaining their biocompatibility. CNT is addressed in terms of sustainable environment and green technologies perspective, such as wastewater treatment, biocompatibility, photocatalytic and green nanocomposites. In this work, we built up a straightforward science strategy to combine Carbon Nanotubes (CNT) adorned with silver tungstate nanoparticles (Ag2WO4) utilizing various strategies. The synthesized nanocomposite is employed for the photocatalytic degradation of Rhodamine-B (RhB) in a single step which resolves organic dye contaminant issues with nontoxic molecular intermediates at the same time, removal of heavy metals. The development of Ag2WO4 with functionalized bunches on the outside of CNT was investigated by X-ray diffraction, TEM, EDS, XPS, The normal size of CNTAg 2WO4 was around 8.26nm with about uniform size appropriation. Antibacterial impact of CNT-Ag2WO4 nanocomposites was assessed against two of Gramnegative pathogenic organisms and two Gram-positive microbes. adjusting the electron microscopy examination on cytotoxic effect of the drug on various cell line & antioxidant component of CNT-Ag2WO4 is a physical connection with cell layer, the enormous development of cells by anti-mitotic potential, and quicker destructibility of cell, henceforth bringing about cells demise can lookout.","PeriodicalId":91210,"journal":{"name":"Austin journal of environmental toxicology","volume":"91 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-03-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78745622","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
S. Gopikumar, S. Sundararajan, Gladston Jak, Kumar Cav, Babu Kh
{"title":"A Quantitative Study of Hydraulic Parameters of Thamirabarani River","authors":"S. Gopikumar, S. Sundararajan, Gladston Jak, Kumar Cav, Babu Kh","doi":"10.26420/AUSTINJENVIRONTOXICOL.2021.1034","DOIUrl":"https://doi.org/10.26420/AUSTINJENVIRONTOXICOL.2021.1034","url":null,"abstract":"Each channel has unique characteristics of its own; River Thamirabarani being no exception. The project proposal aims at investigating the hydraulic geometry of the Thamirabarani River from Pothigai to the downstream up to Srivaikundam [1]. The interrelationship among stream sediment load, channel width and channel depth shows the nature of hydraulic geometry of Thamirabarani River channel. Due to the effect of Papanasam dam the natural flow of this river has been obstructed, and this is resulting in reduced discharge at downstream [2]. Confluences of major tributaries such as Manimuthar, Servalaru, Gadana and Ramanadhi may play significant role for the influx of extra discharge and sediment load to the main river. This extra sediment influx is also responsible for frequent bar formation in the river channel. The input morphometric maps have to be prepared using GIS and Remote Sensing tools like Arc GIS 10.1 and Global Mapper [3].","PeriodicalId":91210,"journal":{"name":"Austin journal of environmental toxicology","volume":"9 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-02-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77602775","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Physico-Chemical Characterization and Assessment of Cytotoxic and Genotoxic Effects of Poly-Ethylene-Glycol Coated and Uncoated Gold Nanoparticles on Human Kidney (HK-2) Cells.","authors":"C R Rogers, S Dasari, A K Patlolla, P B Tchounwou","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Although gold nanoparticles (Au-NPs) have been widely used in medicine for the diagnosis and treatment of patients due to their unique physicochemical properties, chemical stability and biocompatibility, recent reports have also highlighted their potential to induce toxicity to humans. In the present study, we investigated the toxic effects of uncoated and polyethylene glycol (PEG)-coated AuNPs on human kidney (HK-2) cells. Both forms of AuNP were synthesized and characterized using standard protocols. Dynamic Light Scattering (DLS), Zeta Sizer Nano ZS analyzer, Transmission Electron Microscopy (TEM), and Inductively Coupled Plasma-Optical Emission Spectroscopy (ICP-OES) were used to measure their distribution, zeta potential/surface charge, morphological size, and Au concentrations, respectively. Cytotoxicity was measured by Cyto-Tox assay and trypan blue exclusion test. Oxidative stress (OS) was assessed by quantifying the levels of Glutathione (GSH), and Mitochondria Membrane Potential (MMP). Genotoxicity was assessed by single cell gel electrophoresis (Comet assay) and Chromosomal Aberration (CA) assay. Uncoated AuNPs significantly reduced cell viability, increased ROS, decreased GSH, depolarized the MMP, and induced significant DNA damage and chromosomal alterations including chromosome gaps, centric rings, breaks, deletions, and intra and inter-chromosome exchanges, in a concentration-dependent manner. PEG-coated AuNPs displayed lower cytotoxic and genotoxic effects, and did not produce any significant increase in ROS or significant decrease in GSH along with negligible polarization of the MMP. Hence, PEG-coated AuNPs are relatively less toxic than uncoated AuNPs and therefore, may have potential applications in nanomedicine.</p>","PeriodicalId":91210,"journal":{"name":"Austin journal of environmental toxicology","volume":"7 2","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9590441/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141285850","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
C. Munshi, Dr. Rabindra Das, P. Biswas, K. Sen, N. Mondal, S. Mukherjee, R. Bhowmick, P. Kundu, S. Bhattacharya
{"title":"Arsenic Induced Patterns of Auto-Grooming Response Over Time in Macrobrachium lamarrei: A Study on Behavioural Plasticity","authors":"C. Munshi, Dr. Rabindra Das, P. Biswas, K. Sen, N. Mondal, S. Mukherjee, R. Bhowmick, P. Kundu, S. Bhattacharya","doi":"10.21203/rs.3.rs-113016/v1","DOIUrl":"https://doi.org/10.21203/rs.3.rs-113016/v1","url":null,"abstract":"Behavioural toxicity is a very applicable area in the vast field of ecotoxicology where, assessment of the alteration of behaviour is an effective tool to evaluate neural activity in an organism. In our present study, we have shown that arsenic trioxide exposure can stimulate repetitive grooming activity in a freshwater prawn, Macrobrachium lamarrei; however, over time the organism can modulate the grooming performances to less. We predict that repetition in the grooming behaviour is a case of neurotoxicity by arsenic and induction of Autism Spectrum Disorder (ASD) within a short period of exposure time.","PeriodicalId":91210,"journal":{"name":"Austin journal of environmental toxicology","volume":"7 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73094473","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}