电镀废水诱导的Cirrhinus mrigala血液样本细胞病理改变和DNA损伤的检测(Hamilton, 1822)

S. R., B. S, R. S., M. M, M. R, Selvaraj J
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引用次数: 0

摘要

本研究以淡水鱼Cirrhinus mrigala为模型生物,探讨了电镀工业废水(EIE)与水生生态系统氧化应激之间的机制联系。为此,在受控的实验室条件下,将这些鱼暴露在工业废水中10、20和30天。评估各种血液学参数(总红细胞和白细胞、血红蛋白、MCV、MCH、PCV)、生化代谢物(葡萄糖、蛋白质、胆固醇)和遗传毒性标志物(彗星试验和微核试验)。暴露30天的鱼RBC显著增加(p <0.001),同时总白细胞减少。Hb、MCV、MCH、PCV等血细胞指标均显著下降(p <0.001)。彗星试验结果显示,暴露于EIE 30天的鱼的尾长增加,显著增加了红细胞的遗传毒性频率(p < 0.05)。总之,本研究的结果清楚地证明了淡水鱼Cirrhinus mrigala对电镀工业废水在血液学、生化和遗传毒性标志物上的浓度依赖性。本研究结果表明,EIE可能是对鱼类产生生态毒性和遗传毒性作用的潜在外源物质。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Detection on Electroplating Effluent-Induced Cytopathological Alterations and DNA Damage in the Blood Samples of Cirrhinus mrigala (Hamilton, 1822)
In the present study, fresh waterfish Cirrhinus mrigala has been used as a model organism to understand the mechanistic link between electroplating industrial effluent (EIE) and oxidative stress in aquatic ecosystems. To this end, the fishes were exposed to industrial effluent, in the tank water for 10, 20 and 30 days under controlled laboratory conditions. Various hematological parameters (Total RBC and WBC, Hemoglobin, MCV, MCH, PCV), biochemical metabolites (Glucose, protein, Cholesterol) and markers of genotoxicity (comet assay and micronuclear assay) were assessed. Fishes exposed for 30 days showed marked increase (p <0.001) in RBC with concomitant decrease in total WBC. There is significant (p <0.001) decline in indices of blood cells such as Hb, MCV, MCH and PCV. Results from comet assay showed significantly (p < 0.05) increased frequency genotoxicity in erythrocytes of as evident from observed increase in tail length in fishes exposed to EIE for 30 days. Overall, the results of this study, clearly demonstrated concentration dependent response of electroplating industrial effluent on freshwater fish Cirrhinus mrigala on haematological, biochemical and genotoxic markers. The results obtained herein suggests EIE as potential xenobiotics contributing towards ecotoxic and genotoxic effect in fishes.
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