Journal of biomarkers最新文献_第4页

Glucose-6-Phosphate Dehydrogenase Activity and Protein Oxidative Modification in Patients with Type 2 Diabetes Mellitus. 2型糖尿病患者葡萄糖-6-磷酸脱氢酶活性和蛋白氧化修饰

Journal of biomarkers Pub Date : 2013-01-01 Epub Date: 2013-12-22 DOI: 10.1155/2013/430813

Aida A Mahmoud, Amal K A Nor El-Din

{"title":"Glucose-6-Phosphate Dehydrogenase Activity and Protein Oxidative Modification in Patients with Type 2 Diabetes Mellitus.","authors":"Aida A Mahmoud, Amal K A Nor El-Din","doi":"10.1155/2013/430813","DOIUrl":"https://doi.org/10.1155/2013/430813","url":null,"abstract":"Objectives. The aim of the present investigation was to study the activity of glucose-6-phosphate dehydrogenase (G6PD) and correlate its activity to protein oxidation markers in type 2 diabetic patients under poor glycemic control. Methods. G6PD activity, protein carbonyl group concentration, and total thiol group content were measured in blood samples of 40 patients with type 2 diabetes mellitus under poor glycemic control and 20 healthy control subjects. Results. G6PD activity and total thiol group content decreased significantly while glycated hemoglobin (HbA1C) and protein carbonyl group concentration increased significantly in diabetic patients than in the controls (P < 0.001). In addition, Obtained results revealed that, in diabetics, G6PD activity negatively correlated to protein carbonyl and HbA1C (r = -0.77 and -0.65, resp.), while positively correlated to total thiol (r = 0.66) and protein carbonyl negatively correlated to total thiol (r = -0.85), while positively correlated to HbA1C (r = 0.43). Also in controls, G6PD activity negatively correlated to protein carbonyl and HbA1C (r = -0.57 and -0.56, resp.), while positively correlated to total thiol (r = 0.5) and protein carbonyl negatively correlated to total thiol (r = -0.48), while positively correlated to HbA1C (r = 0.68). Conclusions. We concluded that G6PD activity decreased in diabetics than in controls and was negatively correlated to oxidative stress markers and HbA1C. G6PD activity can be taken as a biomarker of oxidative stress and poor glycemic control in type 2 diabetic patients. ","PeriodicalId":91105,"journal":{"name":"Journal of biomarkers","volume":"2013 ","pages":"430813"},"PeriodicalIF":0.0,"publicationDate":"2013-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2013/430813","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"33957302","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 24

T-Cell Response to Hepatitis B Core Antigen: Identification of Prior Exposure to and Confirmatory Testing for Screening for Anti-HBc. t细胞对乙型肝炎核心抗原的反应:先前暴露的鉴定和筛选抗- hbc的确认试验。

Journal of biomarkers Pub Date : 2013-01-01 Epub Date: 2013-12-03 DOI: 10.1155/2013/812170

Patricia Araujo, Roger Y Dodd, Flavia Latinni, Renata Souza, Ricardo Diaz, Jose Augusto Barreto

{"title":"T-Cell Response to Hepatitis B Core Antigen: Identification of Prior Exposure to and Confirmatory Testing for Screening for Anti-HBc.","authors":"Patricia Araujo, Roger Y Dodd, Flavia Latinni, Renata Souza, Ricardo Diaz, Jose Augusto Barreto","doi":"10.1155/2013/812170","DOIUrl":"https://doi.org/10.1155/2013/812170","url":null,"abstract":"Background. During routine donor screening in the blood bank, it is not uncommon to find isolated reactivity for anti-HBc in the absence of detectable HBV DNA in a first donation but absence of reactivity to anti-HBc in subsequent donations, suggesting a false-positive result for anti-HBc. Study Design and Methods. The blood donor population was screened between January 2010 and October 2011. We selected 2,126 donations positive only for anti-HBc from a total of 125,068 donations. During the process, OBI donors were identified, and their HBcAg-specific T-cell response was analyzed and compared to donors with chronic (HBsAg positive) and recovered (anti-HBc only) infection. We analyzed correlations between signal levels (Co/s) in the competitive assay for anti-HBc and HBV DNA detection. Results. In the 21-month study period, 21 blood donors with anti-HBc alone were identified as OBI (1 in each 5955 donors). The relevant finding was the observation that anti-HBc only subjects with Co/s ≥ 0.1 did not have either HBcAg-specific T-cells or detectable HBV DNA and OBI subjects presented with Co/s ≤ 0.1 and HBcAg T-cell response. In the subset of 21 OBI subjects, 9 donors remained positive for HBcAg T-cell response after four collections. In all 9 samples, we observed HBV DNA fluctuation. Conclusion. Our data suggest that HBcAg-specific T-cell response could be used to confirm anti-HBc serological status, distinguishing previous exposure to Hepatitis B virus from anti-HBc false-positive results. ","PeriodicalId":91105,"journal":{"name":"Journal of biomarkers","volume":"2013 ","pages":"812170"},"PeriodicalIF":0.0,"publicationDate":"2013-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2013/812170","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"33959809","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Population-Sequencing as a Biomarker for Sample Characterization. 群体测序作为样品表征的生物标志物。

Journal of biomarkers Pub Date : 2013-01-01 Epub Date: 2013-12-08 DOI: 10.1155/2013/861823

John P Jakupciak

{"title":"Population-Sequencing as a Biomarker for Sample Characterization.","authors":"John P Jakupciak","doi":"10.1155/2013/861823","DOIUrl":"https://doi.org/10.1155/2013/861823","url":null,"abstract":"Sequencing is accepted as the \"gold\" standard for genetic analysis and continues to be used as a validation and reference tool. The idea of using sequence analysis directly for sample characterization has been met with skepticism. However, herein, utility of direct use of sequencing to identify multiple genomes present in samples is presented and reviewed. All samples and \"pure\" isolates are populations of genomes. Population-Sequencing is the use of probabilistic matching tools in combination with large volumes of sequence information to identify genomes present, based on DNA analysis across entire genomes to determine genome assignments, to calculate confidence scores of major and minor genome content. Accurate genome identification from mixtures without culture purification steps can achieve phylogenetic classification by direct analysis of millions of DNA fragments. Genome sequencing data of mixtures can function as biomarkers for use to interrogate genetic content of samples and to establish a sample profile, inclusive of major and minor genome components, drill down to identify rare SNP and mutation events, compare relatedness of genetic content between samples, profile-to-profile, and provide a probabilistic or statistical scoring confidence for sample characterization and attribution. The application of Population-Sequencing will facilitate sample characterization and genome identification strategies. ","PeriodicalId":91105,"journal":{"name":"Journal of biomarkers","volume":"2013 ","pages":"861823"},"PeriodicalIF":0.0,"publicationDate":"2013-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2013/861823","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"33959810","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Neopterin in Diagnosis and Monitoring of Infectious Diseases. 新蝶呤在传染病诊断和监测中的应用。

Journal of biomarkers Pub Date : 2013-01-01 Epub Date: 2013-12-08 DOI: 10.1155/2013/196432

Michael Eisenhut

{"title":"Neopterin in Diagnosis and Monitoring of Infectious Diseases.","authors":"Michael Eisenhut","doi":"10.1155/2013/196432","DOIUrl":"https://doi.org/10.1155/2013/196432","url":null,"abstract":"Neopterin is produced by activated monocytes, macrophages, and dendritic cells upon stimulation by interferon gamma produced by T-lymphocytes. Quantification of neopterin in body fluids has been achieved by standard high-performance liquid chromatography, radioimmunoassays, and enzyme-linked immunosorbent assays. Neopterin levels predict HIV-related mortality more efficiently than clinical manifestations. Successful highly active antiretroviral therapy is associated with a decrease in neopterin levels. Elevated neopterin levels were associated with hepatitis by hepatitis A, B, and C viruses. Serum neopterin levels were found to be a predictor of response to treatment of chronic HCV infection with pegylated interferon combined with ribavirin. Neopterin levels of patients with pulmonary tuberculosis were found to be higher in patients with more extensive radiological changes. Elimination of blood donors with elevated neopterin levels to reduce risk of transmission of infections with known and unknown viral pathogens has been undertaken. Neopterin measurement is hereby more cost effective but less sensitive than screening using polymerase chain reaction based assays. In conclusion neopterin is a nonspecific marker of activated T-helper cell 1 dominated immune response. It may be a useful marker for monitoring of infectious disease activity during treatment and for more accurate estimation of extent of disease and prognosis. ","PeriodicalId":91105,"journal":{"name":"Journal of biomarkers","volume":"2013 ","pages":"196432"},"PeriodicalIF":0.0,"publicationDate":"2013-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2013/196432","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34026654","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 89

Markers of Oxidative Stress during Diabetes Mellitus. 糖尿病期间氧化应激的标志物。

Journal of biomarkers Pub Date : 2013-01-01 Epub Date: 2013-12-17 DOI: 10.1155/2013/378790

Brahm Kumar Tiwari, Kanti Bhooshan Pandey, A B Abidi, Syed Ibrahim Rizvi

引用次数: 427

Amyotrophic Lateral Sclerosis and Metabolomics: Clinical Implication and Therapeutic Approach. 肌萎缩侧索硬化症与代谢组学：临床意义与治疗方法》。

Journal of biomarkers Pub Date : 2013-01-01 Epub Date: 2013-03-14 DOI: 10.1155/2013/538765

Alok Kumar, Devlina Ghosh, R L Singh

引用次数: 0

Prostasin: An Epithelial Sodium Channel Regulator. 前列腺素:上皮钠通道调节剂。

Journal of biomarkers Pub Date : 2013-01-01 Epub Date: 2013-07-02 DOI: 10.1155/2013/179864

Shakti Aggarwal, Pradeep K Dabla, Sarika Arora

{"title":"Prostasin: An Epithelial Sodium Channel Regulator.","authors":"Shakti Aggarwal, Pradeep K Dabla, Sarika Arora","doi":"10.1155/2013/179864","DOIUrl":"https://doi.org/10.1155/2013/179864","url":null,"abstract":"Prostasin is a glycophosphatidylinositol-anchored protein which is found in prostate gland, kidney, bronchi, colon, liver, lung, pancreas, and salivary glands. It is a serine protease with trypsin-like substrate specificity which was first purified from seminal fluid in 1994. In the last decade, its diverse roles in various biological and physiological processes have been elucidated. Many studies done to date suggest that prostasin is one of several membrane peptidases regulating epithelial sodium channels in mammals. A comprehensive literature search was conducted from the websites of Pubmed Central, the US National Library of Medicine's digital archive of life sciences literature and the National Library of Medicine. The data was also assessed from journals and books that published relevant articles in this field. Understanding the mechanism by which prostasin and its inhibitors regulate sodium channels has provided a new insight into the treatment of hypertension and some other diseases like cystic fibrosis. Prostasin plays an important role in epidermal growth factor receptor (EGFR) signal modulation. Extracellular proteases have been implicated in tumor metastasis and local tissue invasion because of their ability to degrade extracellular matrices.","PeriodicalId":91105,"journal":{"name":"Journal of biomarkers","volume":"2013 ","pages":"179864"},"PeriodicalIF":0.0,"publicationDate":"2013-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2013/179864","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34026653","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 14

Effect of Quercetin on Haematobiochemical and Histological Changes in the Liver of Polychlorined Biphenyls-Induced Adult Male Wistar Rats. 槲皮素对多氯联苯诱导成年雄性Wistar大鼠肝脏血液生化及组织学变化的影响。

Journal of biomarkers Pub Date : 2013-01-01 Epub Date: 2012-10-01 DOI: 10.1155/2013/960125

Kandaswamy Selvakumar, Senthamilselvan Bavithra, Sekaran Suganya, Firdous Ahmad Bhat, Gunasekaran Krishnamoorthy, Jagadeesan Arunakaran

{"title":"Effect of Quercetin on Haematobiochemical and Histological Changes in the Liver of Polychlorined Biphenyls-Induced Adult Male Wistar Rats.","authors":"Kandaswamy Selvakumar, Senthamilselvan Bavithra, Sekaran Suganya, Firdous Ahmad Bhat, Gunasekaran Krishnamoorthy, Jagadeesan Arunakaran","doi":"10.1155/2013/960125","DOIUrl":"https://doi.org/10.1155/2013/960125","url":null,"abstract":"Polychlorinated biphenyls exposure damages the rat liver cells. Hematological parameters such as hemoglobin, packed cell volume, red-blood cells, white-blood cells, neutrophils, platelet counts, and RBC indices were significantly decreased. Polymorphs, eosinophil counts, and erythrocyte sedimentation rate were significantly increased. Serum liver enzymes such as aspartate transaminase, alanine transaminase, alkaline phosphatase, and gamma-glutamyl transferase were increased by PCBs treatment. Serum lipid profiles such as cholesterol, triglycerides, low-density lipoproteins and very-low-density lipoproteins were increased in PCBs-treated rats. High-density lipoprotein, total protein, albumin, globulin levels, and albumin/globulin ratio were also decreased after PCB exposure. Then levels of sodium, potassium, chloride, and bicarbonate were also altered. Serum glucose levels were increased along with total bilirubin after PCBs exposure. Simultaneous quercetin supplementation significantly protected the PCBs-induced changes of hematobiochemical parameters. Thus, quercetin shows a protective role against PCBs-induced alterations in the hematological and biochemical parameters. ","PeriodicalId":91105,"journal":{"name":"Journal of biomarkers","volume":"2013 ","pages":"960125"},"PeriodicalIF":0.0,"publicationDate":"2013-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2013/960125","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"33959811","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 51