Biological Research最新文献

{"title":"Impact of Salvadora persica aqueous extract on follicular development in female rats.","authors":"Sabreen M Ghareeb, Mahmoud Abd-Elkareem, Ahmed Abou-Elmagd","doi":"10.1186/s40659-026-00711-z","DOIUrl":"10.1186/s40659-026-00711-z","url":null,"abstract":"<p><p>Salvadora persica (miswak) has many useful biological activities. Some studies have reported that miswak can be used as a contraceptive agent. However, no available investigations explain the histomorphological structure of ovarian follicles after miswak aqueous extract (MAE) administration. Twelve female Wistar albino rats were divided into two equal groups. In the control group (CG), the animals received normal saline daily for 4 weeks. While in the miswak treated group (MTG), the animals received orally 900 mg/kg of body weight of the MAE daily for the same period. At the end of the experiment, the rats were anesthetized and then euthanized by cervical dislocation. The ovaries were dissected, removed, weighted, fixed, and processed for histological examination by light and electron microscopy.Results revealed that the ovaries of CG showed various stages of follicular development. While in the MTG, the ovaries exhibited follicular atresia. The immunoexpression of caspase-3, progesterone receptors (PR), and estrogen receptors alpha (ERA) in the MTG and CG were reported.Our preliminary animal research indicates that MAE can inhibit follicular development and induce follicular atresia. These findings suggest a potential antifollicular and/or antiovulatory effect that requires confirmation through dose-response, fertility, toxicity, and clinical studies.</p>","PeriodicalId":9084,"journal":{"name":"Biological Research","volume":"59 1","pages":""},"PeriodicalIF":5.4,"publicationDate":"2026-06-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13285512/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148300816","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrated morphological, pomological, biochemical, and ISSR-based assessment of genetic diversity in wild sour orange (Citrus aurantium L.) genotypes from the Hassa district of Hatay, Türkiye.","authors":"Yazgan Tunç","doi":"10.1186/s40659-026-00708-8","DOIUrl":"10.1186/s40659-026-00708-8","url":null,"abstract":"<p><p>Sour orange (Citrus aurantium L.) is a hybrid species of considerable agronomic, medicinal, and industrial importance; however, its wild genetic resources remain largely underexplored. This study evaluated 24 naturally occurring wild sour orange genotypes collected from the Hassa district of Hatay, Türkiye, using an integrated framework combining morphological, pomological, biochemical, and ISSR molecular markers. The combined use of multi-trait phenotypic measurements and molecular markers enabled a comprehensive assessment of genetic and phenotypic diversity within this localized germplasm. Substantial variation was observed in fruit and leaf traits, indicating strong potential for the selection of contrasting ideotypes. Biochemical analyses revealed marked diversity in total phenolic and flavonoid contents, antioxidant capacity, and vitamin C levels, allowing the identification of genotypes that combine high nutritional quality with commercially acceptable fruit size. ISSR analysis produced a high polymorphism rate (75.72%), confirming pronounced genetic diversity within this micro-region. Multivariate analyses, including correlation, regression, principal component analysis, and hierarchical clustering visualized through heat maps, clearly discriminated genotypes according to fruit size, seed productivity, and phytochemical composition. Overall, the results highlight the presence of considerable genetic diversity within the Hassa district; however, given the geographically restricted sampling, these findings should be interpreted as representative of a localized germplasm rather than the entire species distribution. The study provides a practical basis for selecting superior genotypes for rootstock breeding, fresh fruit utilization, and nutraceutical applications.</p>","PeriodicalId":9084,"journal":{"name":"Biological Research","volume":" ","pages":""},"PeriodicalIF":5.4,"publicationDate":"2026-06-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13495391/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148249112","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Generation of a stable mouse endometrial tumor-derived cell line using conditional reprogramming.","authors":"Tang Ansu Zhang, Rong Zhao, Yu Zhang, Hongbo Wang","doi":"10.1186/s40659-026-00706-w","DOIUrl":"10.1186/s40659-026-00706-w","url":null,"abstract":"<p><strong>Background: </strong>Endometrial cancer (EC) is a common malignancy of the female reproductive tract worldwide. While comprehensive genomic analyses have identified multiple genetic alterations in EC, disease models remain relatively rare, limiting the ability to conduct disease studies. Establishing a hybrid in vitro and in vivo model of EC and comparing it to its in vivo counterpart could greatly advance research into EC mechanisms and treatment strategies.</p><p><strong>Methods: </strong>We established genetically engineered EC mice (Pten ^flox/+; Stk11 ^flox/flox; LTF-Cre (PSC)) and constructed a corresponding epithelial cell line from isolated uterine tumor tissues using conditional reprogramming techniques. The cell line was successfully passaged, frozen, and stored in a biobank, providing a valuable resource for future EC research.</p><p><strong>Results: </strong>The PSC-derived cell line stably retained the mutant alleles of Pten and Stk11 during passaging, preserving the genetic profile of the original tumor. In addition, we performed various functional assays on isolated mouse cell linesand formed tumors after subcutaneous implantation of cells in immunodeficient mice and C57BL/6 mice with an intact immune system. Hematoxylin and eosin (H&E) staining and immunohistochemical staining were used to characterize the histopathology and biomarkers of the subcutaneously implanted tumors, which were highly similar to those of the parental mouse uterine tumors.</p><p><strong>Conclusions: </strong>The isolated mouse cell line provides a powerful platform for basic and translational EC studies, matching well with the corresponding in vivo models. The fidelity of the cell line to human-relevant genetic alterations and tumor biology makes it a valuable tool for investigating EC pathogenesis and evaluating novel therapeutic approaches.</p>","PeriodicalId":9084,"journal":{"name":"Biological Research","volume":" ","pages":""},"PeriodicalIF":5.4,"publicationDate":"2026-06-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13492030/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148223095","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Regulation of trophoblast fusion dysfunction in preeclampsia by the NCK1/EIF3D/PPARG axis.","authors":"Ying Shen, Xiaolin Gao, Jiashi Wang, Yingying Hao","doi":"10.1186/s40659-026-00704-y","DOIUrl":"10.1186/s40659-026-00704-y","url":null,"abstract":"<p><strong>Background: </strong>Abnormal syncytialization of cytotrophoblast (CTB) cells is known to be associated with preeclampsia (PE), however, the underlying molecular mechanisms remain elusive.</p><p><strong>Results: </strong>In this study, proteomic analysis of placental tissues was performed to identify proteins involved in PE pathogenesis. NCK Adaptor Protein 1 (NCK1) was found to be downregulated in placental tissues from patients with early-onset PE. In vitro functional assays revealed that NCK1 was markedly increased during spontaneous syncytialization of CTB cells. NCK1 overexpression alleviated hypoxia-induced defects in CTB cell fusion. Mechanistically, NCK1 upregulated both the expression and stability of Peroxisome Proliferator-Activated Receptor Gamma (PPARG), accompanied by increased m6A modification of its mRNA. Overexpression of PPARG significantly rescued hypoxia-induced syncytialization impairment, whereas PPARG deficiency abolished the promotive effects of NCK1 on CTB syncytialization. Additionally, phosphorylation of Eukaryotic Translation Initiation Factor 3 Subunit (EIF3D) was significantly increased during spontaneous syncytialization, and NCK1 overexpression partially reversed hypoxia-induced suppression of EIF3D phosphorylation. EIF3D knockdown significantly reduced m6A levels on PPARG mRNA, likely due to impaired NCK1-mediated suppression of Alpha-Ketoglutarate-Dependent Homolog 5 (ALKBH5), an m6A demethylase. Furthermore, NCK1 enhanced EIF3D phosphorylation by inhibiting its O-GlcNAcylation.</p><p><strong>Conclusions: </strong>Our findings demonstrate that NCK1 stabilizes PPARG by modulating the crosstalk between O-GlcNAcylation and phosphorylation of EIF3D, thereby restoring hypoxia-impaired syncytialization in CTB cells. This study identifies the NCK1/EIF3D/PPARG axis as a molecular pathway potentially relevant to trophoblast fusion dysfunction in PE.</p>","PeriodicalId":9084,"journal":{"name":"Biological Research","volume":" ","pages":""},"PeriodicalIF":5.4,"publicationDate":"2026-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13479735/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148216246","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Developmental and functional landscape of theca cells in ovarian biology.","authors":"Mahnaz Haddadi, Samane Adib, Christiani A Amorim, Azam Dalman, Mojtaba Rezazadeh Valojerdi","doi":"10.1186/s40659-026-00680-3","DOIUrl":"https://doi.org/10.1186/s40659-026-00680-3","url":null,"abstract":"<p><p>Ovarian theca cells constitute essential components of the follicular microenvironment and play central roles in follicular development, steroidogenesis, and endocrine regulation. Despite their significance, the developmental origins, differentiation processes, and functional dynamics of theca cells remain incompletely defined, particularly in humans. This review provides an updated synthesis of current knowledge on the ontogeny, molecular signaling pathways, and intercellular interactions of theca cells. It also presents recent findings on the role of theca stem or progenitor cells and their relevance to reproductive disorders, including polycystic ovary syndrome (PCOS), hyperthecosis, and ovarian insufficiency. A comprehensive literature search was conducted using PubMed, Scopus, and Web of Science through May 2025. Search terms included \"theca cells,\" \"theca progenitors,\" \"follicular development,\" \"ovarian differentiation,\" \"steroidogenesis,\" and \"reproductive disorders.\" Original research articles and reviews providing mechanistic insights were included. Current evidence indicates that several signaling pathways, including IGF, SCF, FGF, members of the TGF-β superfamily, GDF9, BMPs, and Hedgehog proteins, are involved in the recruitment, proliferation, and differentiation of theca cells. Bidirectional communication among oocytes, granulosa cells, and theca cells remains essential for folliculogenesis. Increasing evidence links theca cell dysfunction to the pathophysiology of PCOS, primary ovarian insufficiency, and reproductive aging. The identification of theca stem cells (TSCs) and their proposed roles in ovarian regeneration represents an important conceptual advance. A deeper understanding of theca cell biology may guide the development of targeted strategies for infertility and ovarian dysfunction. In contrast, the integration of TSC biology offers new directions for reproductive medicine and reproductive health.</p>","PeriodicalId":9084,"journal":{"name":"Biological Research","volume":" ","pages":""},"PeriodicalIF":4.6,"publicationDate":"2026-06-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148204075","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Study on the mechanism of erectile dysfunction in D-galactose induced aging rats: the construction of aging model.","authors":"Taotao Sun, Yipiao Liu, Wenjia Deng, Yinwei Chen, Wenchao Xu, Penghui Yuan","doi":"10.1186/s40659-026-00705-x","DOIUrl":"10.1186/s40659-026-00705-x","url":null,"abstract":"<p><strong>Background: </strong>The incidence of erectile dysfunction (ED) rises sharply with age, which has brought a huge social burden. The exploration of aging-related ED is hindered due to the limitations of natural aging models. As an ideal inducer of accelerated aging models, the mechanism of D-galactose (D-gal) in ED has not been fully elucidated. Therefore, this study aimed to verify the feasibility of aging-related ED induced by D-gal and explore its potential mechanism.</p><p><strong>Methods: </strong>Primary corpus cavernosum smooth muscle cells (CCSMCs) and human umbilical vein endothelial cells (HUVECs) were incubated with concentration gradients of D-gal. Meanwhile, aging rat models were established via intraperitoneal and subcutaneous injection of D-gal, and the erectile function of all subjects was measured by electrical stimulation. Samples from both cells and animals were then collected for subsequent detection.</p><p><strong>Results: </strong>Cellular senescence was confirmed in both cells and was accompanied by oxidative stress, mitochondrial dysfunction, and cell cycle arrest in vitro. The erectile function of rats treated with D-gal was affirmed to be impaired compared with normal rats. In addition to the above-mentioned pathological alterations, tissue fibrosis was also associated with D-gal administration in vivo. Moreover, no significant difference was found between the two administration methods in inducing senescence of rats.</p><p><strong>Conclusions: </strong>D-gal could promote the progression of aging-related ED, accompanied by the exacerbation of oxidative stress, mitochondrial dysfunction, cell cycle arrest, and fibrosis. Our data suggested that D-gal may be an ideal mediator for the induced model of aging-related ED, which could facilitate preclinical exploration of this disease.</p>","PeriodicalId":9084,"journal":{"name":"Biological Research","volume":" ","pages":""},"PeriodicalIF":5.4,"publicationDate":"2026-06-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13471410/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148204064","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"From in vitro to in vivo: hypoxia attenuates replicative senescence and preserves therapeutic activity in long-term passaged human umbilical cord-derived mesenchymal stem cells.","authors":"Chenxi Jia, Ze Lin, Tianrui Zhang, Lingbin He, Yueyang Li, Jianhong Li, Xinyi Li, Yuan Zhang, Bozhi Cai, Muping Zou, Libiao Wu, Xutao Zhao, Chunhong Ren, Xin Zhang","doi":"10.1186/s40659-026-00703-z","DOIUrl":"10.1186/s40659-026-00703-z","url":null,"abstract":"<p><strong>Background: </strong>Mesenchymal stem cells (MSCs) hold great potential for regenerative medicine and tissue engineering. However, their clinical use is limited by low in vivo availability and rapid senescence during ex vivo expansion, posing a major challenge for scaled production. Growing evidence indicates that microenvironmental oxygen tension critically regulates MSCs fate. This study aims to systematically evaluate how different oxygen tensions modulate MSCs aging and function, providing evidence to optimize MSCs expansion protocols for clinical applications.</p><p><strong>Methods: </strong>Human umbilical cord derived-mesenchymal stem cells (hUC-MSCs) were maintained under normoxic (20%O₂) and hypoxic (5%O₂) conditions through continuous passaging until senescence occurred. In vitro analysis systematically compared proliferation, cell cycle distribution, apoptosis, and senescence biomarkers across serially expanded populations from both oxygen concentrations. For in vivo evaluations, a type 2 diabetic nephropathy mouse model was used to investigate therapeutic efficacy. Mice received systemic transplantation of hUC-MSCs from each group, and renal functional recovery along with histopathological changes were assessed.</p><p><strong>Results: </strong>Cells cultured under both 20%O₂ (normoxia) and 5%O₂ (hypoxia) stably expressed MSC-specific surface markers throughout serial passages. Hypoxia-cultured cells retained enhanced adipogenic and osteogenic differentiation potential, along with superior proliferative capacity and growth factor secretion, through at least 16 passages, consistently outperforming normoxic counterparts. Hypoxic conditions also attenuated replicative senescence, suppressed DNA damage, and reduced secretion of SASP-associated pro-inflammatory factors (including IL-6, IL-8, and TGF-β), while decreasing apoptosis. In a diabetic nephropathy mouse model, P16 MSCs cultured under hypoxia still significantly improved renal function and attenuated fibrosis post-transplantation, as demonstrated by reduced 24-h urinary protein, serum creatinine, and blood urea nitrogen levels compared to the normoxic MSCs group.</p><p><strong>Conclusion: </strong>We provide the first in vivo evidence that hypoxic culture conditions enables high-passage hUC-MSCs(P16) to retain therapeutic potency in diabetic nephropathy. Our findings demonstrate that hypoxia effectively mitigates replicative senescence through suppression of oxidative damage and SASP secretion, thereby preserving the proliferative capacity and paracrine repair functions of long-term cultured cells.</p>","PeriodicalId":9084,"journal":{"name":"Biological Research","volume":" ","pages":""},"PeriodicalIF":5.4,"publicationDate":"2026-06-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13450261/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148148395","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction: INO80 regulates promoter-associated R-loops to coordinate transcription and maintain genome stability in embryonic stem cells.","authors":"Hoseong Lim, Ohbeom Kwon, Hyeonwoo La, Hyeonji Lee, Heeji Lee, Jeong-Tae Do, Hyuk Song, Youngsok Choi, Kwonho Hong","doi":"10.1186/s40659-026-00701-1","DOIUrl":"10.1186/s40659-026-00701-1","url":null,"abstract":"","PeriodicalId":9084,"journal":{"name":"Biological Research","volume":"59 1","pages":""},"PeriodicalIF":4.6,"publicationDate":"2026-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13217756/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148052558","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrative morphological, biochemical, and plastome-based characterization of a wild plum (Prunus spp.) population from Mount Erciyes (Central Anatolia, Türkiye).","authors":"Samuel Obedgiu, Kahraman Gürcan, Nurdan Tuna Güneş, Ahmet Sümbül, Mehmet Yaman, Vahid Roumi, Yazgan Tunç","doi":"10.1186/s40659-026-00702-0","DOIUrl":"10.1186/s40659-026-00702-0","url":null,"abstract":"<p><p>Plums (Prunus spp.) are among the most diverse fruit tree species, yet their wild relatives remain poorly characterized despite their importance for breeding, conservation, and understanding evolutionary diversity. Here, we investigated a wild plum population from Mount Erciyes, located in Central Anatolia, Türkiye, through integrated morphological, textural, total content-based biochemical, and plastome analyses. Floral morphology of the Erciyes wild plums displayed intermediate features between apricot (P. armeniaca) and cultivated plum (P. domestica), while fruit texture exhibited significantly higher peel hardness and flesh resistance compared with the reference cultivar 'Papaz Eriği'. Illumina sequencing of the chloroplast genome produced a 157,840 bp plastome with a conserved quadripartite structure, encoding 125 genes. Comparative analyses revealed minor structural differences in the LSC and SSC regions, whereas the IR regions remained highly conserved. Phylogenetic reconstruction based on complete plastomes clustered the Erciyes wild plums with P. domestica and P. cerasifera, reflecting plastome-level phylogenetic affinity. This study identified significant phenotypic and total content-based biochemical diversity in wild plum genotypes. Key traits such as fruit and seed weight, color, and bioactive compound contents showed wide variability. Based on the heatmap hierarchical clustering analysis, the genotypes and the examined traits were divided into two main groups exhibiting significant positive and negative correlations among the traits. Together, these findings provide a characterization of a previously undocumented wild plum from Mount Erciyes and establish a baseline reference for future studies on plum evolution, conservation, and breeding.</p>","PeriodicalId":9084,"journal":{"name":"Biological Research","volume":" ","pages":""},"PeriodicalIF":5.4,"publicationDate":"2026-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13404573/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148040972","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Knockout of filamin A in KGN granulosa tumor cells impairs proliferation, cell cycle progression, migration, and cytoskeletal organization under mechanical stress.","authors":"Yuhao Jiang, Karolina Magdalena Caban, Mirko Peitzsch, Carola Herrmann, Doris Mayr, Jan Bernd Stöckl, Thomas Fröhlich, Artur Mayerhofer, Annette Müller-Taubenberger, Harald Welter","doi":"10.1186/s40659-026-00686-x","DOIUrl":"10.1186/s40659-026-00686-x","url":null,"abstract":"<p><strong>Background: </strong>Filamin A (FLNA) is an actin-binding protein that regulates mechanosensitivity and functions as an intracellular signaling scaffold in various cell types. It has also been implicated in tumor growth. We recently reported FLNA expression in human ovarian granulosa cells and in KGN cells, a granulosa cell tumor (GCT) line.</p><p><strong>Results: </strong>Immunohistochemistry analysis of 51 GCT samples revealed heterogeneous FLNA expression, with approximately 20% showing weak, 18% strong, and the majority moderate expression. We therefore conducted functional studies in KGN cells using CRISPR/Cas9 gene editing. A proteomic approach revealed marked changes in protein abundance upon FLNA depletion: proteins with increased abundance were predominantly related to adhesion, cytoskeletal organization, regulation of cell shape, and lipid metabolic process, whereas those with decreased abundance were associated with DNA replication, cell division, and cell cycle regulation. FLNA-knockout cells showed enlarged cell sizes, reduced proliferation, and slightly affected steroidogenesis. Disruption of FLNA further reduced migration velocity, altered actin cytoskeletal alignment under flow, and modified expression of genes involved in cytoskeletal architecture, adhesion, and mechanosensing under shear stress.</p><p><strong>Conclusions: </strong>Our results identify crucial roles of FLNA in shaping the cellular architecture, motility, and proliferation of KGN cells. Consequently, alterations in FLNA expression may influence intracellular signaling, and responsiveness to mechanical cues in both physiological and pathological contexts.</p>","PeriodicalId":9084,"journal":{"name":"Biological Research","volume":" ","pages":""},"PeriodicalIF":5.4,"publicationDate":"2026-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13200416/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147980358","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}