Biological Research最新文献

{"title":"Seasonal expression of reproductive axis-related neuroendocrine genes and their relation with ovarian maturation in captive yellowtail kingfish (Seriola lalandi).","authors":"Jaime Palomino, Ayleen Olea, Stevanie Ramírez, Phillip Dettleff, Ricardo D Moreno","doi":"10.1186/s40659-025-00622-5","DOIUrl":"10.1186/s40659-025-00622-5","url":null,"abstract":"<p><p>The yellowtail kingfish (Seriola lalandi) is a key species for the diversification of Chilean aquaculture. While controlled reproduction is essential for reliable fish production, the physiological and molecular bases underlying its reproductive cycle remain insufficiently explored. This study aimed to assess the expression patterns of brain-expressed neuroendocrine mRNAs involved in the activation of brain-pituitary-gonad (BPG) axis throughout different year seasons and to correlate them with ovarian maturation stages in S. lalandi females under captive conditions. Reproductive stages were determined by ovarian histology and gonadosomatic index (GSI) analysis. Expression levels of GnRH1 and GnRH2 genes (gnrh1 and gnrh2) as well as melatonin (mtn1ra), dopamine (drd2a), and kisspeptin (kiss1r) receptors were quantified by real time PCR in brain samples from adult individuals. Fish were maintained in temperature- and photoperiod-controlled tanks simulating the four seasons. Histological and GSI analyses identified four distinct reproductive stages. Gene expression peaked in winter and decreased in autumn, aligning with the seasonal progression of ovarian development. These results are consistent with the expected activation of the reproductive axis during the winter months in S. lalandi. Notably, the elevated expression of drd2a in winter suggests that modulation of GnRH action on pituitary function may not rely solely on dopaminergic inhibition. Taken together, our findings indicate that S. lalandi exhibits reproductive dynamics under captivity that mirror those of wild populations, supporting its use as a reliable model for studying reproductive physiology. Moreover, its responsiveness to environmental cues under controlled conditions enables the development of experimental strategies that would be logistically unfeasible in the wild.</p>","PeriodicalId":9084,"journal":{"name":"Biological Research","volume":"58 1","pages":"55"},"PeriodicalIF":4.6,"publicationDate":"2025-08-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12333068/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144798160","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction: Relationship between seawater temperature, brain GnRH-like peptide expression, and gonadal development in wild bigfin reef squid (Sepioteuthis lessoniana).","authors":"Umina Kubo, Jaewoo Lee, Ryosuke Murata, Takashi Aoshima, Yuji Mushirobira, Kiyoshi Soyano","doi":"10.1186/s40659-025-00632-3","DOIUrl":"10.1186/s40659-025-00632-3","url":null,"abstract":"","PeriodicalId":9084,"journal":{"name":"Biological Research","volume":"58 1","pages":"54"},"PeriodicalIF":4.6,"publicationDate":"2025-08-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12317463/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144764543","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Variations in flanking or less conserved positions of Reb1 and Abf1 consensus binding sites lead to major changes in their ability to modulate nucleosome sliding activity.","authors":"Fernanda Raiqueo, Roberto Amigo, José L Gutiérrez","doi":"10.1186/s40659-025-00627-0","DOIUrl":"10.1186/s40659-025-00627-0","url":null,"abstract":"<p><strong>Background: </strong>Maintenance of nucleosome-free regions at gene regulatory regions conform a relevant aspect within chromatin dynamics. In the yeast Saccharomyces cerevisiae, Reb1 and Abf1 are among the transcriptions factors that perform this molecular function. These factors are thought to act as a barrier to nucleosome sliding that chromatin remodeling complexes such as ISW1a perform towards this region, being binding affinity a critical feature to act as a barrier. In this regard, sequence variations at positions flanking transcription factor binding sites could affect DNA shape features and, in turn, binding strength. In addition, recent studies have shown that positions of low conservation and/or flanking sequences might vary from gene bodies to gene regulatory regions. Considering these issues, we aimed to analyze whether variations in flanking or less conserved positions of Reb1 and Abf1 target sequences affect their binding affinity, especially dwell time, and their ability to hinder ISW1a's sliding activity.</p><p><strong>Results: </strong>We found that sequence changes at these positions deeply affect binding strength, particularly dwell time, and the ability to hinder ISW1a's sliding activity. Importantly, even under conditions where a markedly higher transcription factor concentration for a weak binding site was used to compare it to a strong binding site under an equal binding saturation level, the strong site displayed a significantly higher ability to hinder sliding activity. Moreover, genome-wide analyses showed that the sequence variants of Reb1 and Abf1 binding sites conferring this ability to hinder sliding activity to these factors are enriched at promoter regions relative to gene bodies.</p><p><strong>Conclusions: </strong>Our findings show that dwell time is a key feature to hinder nucleosome sliding activity. For Reb1 and Abf1 factors, sequence variation at less conserved positions of their binding sites strongly affects this feature. The differential frequency at these positions found at promoter regions, relative to gene bodies, highlights the relevance of including this type of comparison in certain strategies used to determine the consensus binding site for transcription factors. To determine the molecular functions that require long dwell times and the transcription factors responsible for these tasks will significantly contribute to untangle the grammar of cis-regulatory elements.</p>","PeriodicalId":9084,"journal":{"name":"Biological Research","volume":"58 1","pages":"53"},"PeriodicalIF":4.6,"publicationDate":"2025-07-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12305957/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144741170","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Targeting EGR1-ATF3 signaling mitigates paravertebral muscle degeneration by regulating cell death and inflammaging.","authors":"Xuke Wang, Qingfeng Wang, Zhe Wang, Yingjie Zhou, Xiaobing Jiang, Yongjin Li","doi":"10.1186/s40659-025-00634-1","DOIUrl":"10.1186/s40659-025-00634-1","url":null,"abstract":"","PeriodicalId":9084,"journal":{"name":"Biological Research","volume":"58 1","pages":"52"},"PeriodicalIF":4.6,"publicationDate":"2025-07-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12302741/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144727766","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular basis for the biosynthesis of the siderophore coprogen in the cheese-ripening fungus Penicillium roqueforti.","authors":"Kathia González, Mariana Montanares, Matías Gallardo, Carlos Gil-Durán, Abel M Forero, Jaime Rodríguez, Carlos Jiménez, Inmaculada Vaca, Renato Chávez","doi":"10.1186/s40659-025-00633-2","DOIUrl":"10.1186/s40659-025-00633-2","url":null,"abstract":"<p><strong>Background: </strong>Iron is an essential nutrient for microorganisms, including fungi, which have evolved strategies to acquire it. The most common strategy is the secretion of siderophores, low-molecular-weight compounds with a high affinity for ferric ions, which are involved in cellular iron uptake. Penicillium roqueforti, the fungus responsible for the ripening of blue-veined cheeses, produces coprogen, a hydroxamate-type siderophore. However, to date, the molecular basis for its biosynthesis remains elusive.</p><p><strong>Results: </strong>In this study, we identified and characterized a biosynthetic gene cluster (BGC) responsible for coprogen biosynthesis in P. roqueforti, named the cop BGC. This BGC contains seven genes, three of which (copA, copB and copE) encode enzymes directly involved in coprogen biosynthesis from precursors molecules. Using CRISPR-Cas9, we targeted these three genes and analyzed the resulting mutants by Liquid Chromatography/High-Resolution Mass Spectrometry (LC/HRMS). Our results confirmed that all three genes are necessary for coprogen biosynthesis. Phenotypically, the mutants displayed growth differences under iron-deficient conditions, which correlated with their ability to either synthesize or fail to synthesize coprogen B and dimerumic acid, intermediates in the coprogen pathway with siderophore activity.</p><p><strong>Conclusions: </strong>The results obtained in this work provide important insights into the molecular basis of coprogen biosynthesis in P. roqueforti, enhancing the understanding of how siderophores enable this fungus to thrive in iron-deficient environments.</p>","PeriodicalId":9084,"journal":{"name":"Biological Research","volume":"58 1","pages":"51"},"PeriodicalIF":4.3,"publicationDate":"2025-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12278577/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144681985","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction: Citrus aurantium L. and Citrus latifolia extracts as alternative control agents for Aedes aegypti (Diptera: Culicidae).","authors":"Andrea Martínez Gordon, Alejandro Figueredo López, Ingrid Dayana Jiménez, Laura Barrera Martínez, Oscar H Pardo Cuervo, Nidya Alexandra Segura Guerrero","doi":"10.1186/s40659-025-00631-4","DOIUrl":"10.1186/s40659-025-00631-4","url":null,"abstract":"","PeriodicalId":9084,"journal":{"name":"Biological Research","volume":"58 1","pages":"50"},"PeriodicalIF":4.3,"publicationDate":"2025-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12273323/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144667040","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exposure to polystyrene nanoparticles leads to dysfunction in DNA repair mechanisms in Caco-2 cells.","authors":"Agata Kustra, Mirosław Zając, Piotr Bednarczyk, Kamila Maliszewska-Olejniczak","doi":"10.1186/s40659-025-00629-y","DOIUrl":"10.1186/s40659-025-00629-y","url":null,"abstract":"<p><strong>Background: </strong>Recent studies have highlighted the critical health implications of environmental exposure to nanoplastics, particularly concerning their effects on human gastrointestinal cells. In this study, we used human colorectal adenocarcinoma (Caco-2) cells to investigate the exposure of polystyrene nanoparticles (PNPs) to cellular processes and DNA repair.</p><p><strong>Methods: </strong>We exposed Caco-2 cells to various concentrations of PNPs and monitored cytotoxicity, ROS levels, PARP-1-dependent apoptosis, DNA damage, and changes in DNA damage response (DDR) gene expression.</p><p><strong>Results: </strong>The results indicated that although PNPs did not directly cause SSBs or DSBs, as evidenced by comet assays and γH2AX staining, they induced oxidative stress and significantly altered the expression of genes required for DDR. In particular, critical genes involved in the base excision repair (BER) pathway and DSBs repair were downregulated, suggesting a potential impairment of the cell's ability to repair oxidative DNA damage.</p><p><strong>Conclusions: </strong>This study highlights the sublethal effects of nanoplastics on intestinal barrier cells. It underscores the possible risks of exposure to these environmental contaminants, which can lead to genome instability and other long-term health consequences.</p>","PeriodicalId":9084,"journal":{"name":"Biological Research","volume":"58 1","pages":"49"},"PeriodicalIF":4.3,"publicationDate":"2025-07-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12247316/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144616189","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chinese herbal formula Huayu-Qiangshen-Tongbi decoction ameliorates rheumatoid arthritis through enhancing the release of exosomal miR-125b-5p derived from adipose-derived stem cells by CD63.","authors":"Wu Xiao-Dong, Liang You-Bang, Niu Yun-Bao, Yang Yi-Hong, Huang Wen-Zhi, Wang Mao-Jie, Mei Li-Yan, Gao Kai-Xin, Huang Run-Yue, Chen Xiu-Min","doi":"10.1186/s40659-025-00628-z","DOIUrl":"10.1186/s40659-025-00628-z","url":null,"abstract":"<p><strong>Background: </strong>Rheumatoid arthritis (RA) is a chronic and multifactorial inflammatory disease inducing damages in joints and extra-articular organs. Our previous study has revealed that Huayu-Qiangshen-Tongbi decoction (HQT) ameliorates RA through upregulating microRNA (miRNA) miR-125b-5p to suppress inflammation in rheumatoid fibroblast-like synoviocytes (FLSs). However, the mechanism of HQT increasing miR-125b-5p level in FLSs remains unclear. It has been reported that exosomal miR-125b-5p derived from adipose-derived stem cells (ADSCs) could ameliorate various diseases, yet the effect of exosomal miR-125b-5p derived from ADSCs on FLSs in RA under HQT treatment is largely unknown. The aim is to investigate whether HQT upregulated miR-125b-5p in FLSs through modifying exosomal miR-125b-5p derived from ASCs.</p><p><strong>Methods: </strong>Here, HQT-containing serum was prepared, co-culture of human FLS MH7A cells with ADSCs was performed, gene knockdown in ADSCs was assessed by short hairpin RNA (shRNA), protein degradation was identified after cycloheximide (CHX) treatment and ADSC-derived exosomes were collected to incubate MH7A cells and inject into RA rat model.</p><p><strong>Results: </strong>HQT elevates lipopolysaccharide (LPS)-reduced miR-125b-5p level in FLSs by enhancing the secretion of exosomal miR-125b-5p derived from ADSCs. Besides, HQT attenuates inflammation of FLSs in RA by exosomal miR-125b-5p derived from ADSCs in vitro and in vivo. Mechanistically, HQT suppresses CD63 degradation in ADSCs to facilitate the release of exosomal miR-125b-5p derived from ADSCs.</p><p><strong>Conclusion: </strong>In summary, these findings reveal the mechanism of HQT elevating miR-125b-5p expression in FLSs and provide novel therapeutic strategy for RA treatment.</p>","PeriodicalId":9084,"journal":{"name":"Biological Research","volume":"58 1","pages":"47"},"PeriodicalIF":4.3,"publicationDate":"2025-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12243140/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144607306","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pharmacological evaluation of physcion as a TRPV1 inhibitor with multimodal analgesic efficacy in experimental pain models.","authors":"Hanbin Chen, Guanghong Li, Lin Deng, Shuli Li, Songqiang Huang, Simon Ming-Yuen Lee, Xiaowei Nie, Jin-Song Bian","doi":"10.1186/s40659-025-00630-5","DOIUrl":"10.1186/s40659-025-00630-5","url":null,"abstract":"<p><strong>Background: </strong>Pain serves as a vital protective mechanism triggered by tissue damage. While NSAIDs and opioids offer relief, their prolonged usage is hindered by adverse effects. Developing analgesics with fewer side effects is crucial for effective pain treatment. The TRPV1 channel is a key target for pain relief, with its inhibitors effectively reducing hyperalgesia in animals. This research utilized virtual screening to identify TRPV1-selective natural compounds for potent analgesic properties.</p><p><strong>Results: </strong>The physcion exhibited the notable affinity for TRPV1 compared to the compounds examined. After conducting molecular dynamics simulations, physcion emerged as the compound demonstrating the highest binding affinity towards TRPV1, a finding corroborated by calcium imaging, which validated its inhibitory impact. Furthermore, physcion mitigated the stretch number in the acetic acid-induced stretching model, prolonged the latency period in the hot water tail-flick and hot plate assays, and heightened the pain withdrawal threshold lowered by complete Freund's adjuvant (CFA). Notably, physcion exerted a marked effect in ameliorating bone cancer-induced pain in the hot plate and von Frey tests. Additionally, physcion diminished the levels of inflammatory cytokines and the mRNA expression of both inflammatory and calcium-related genes in the CFA-induced murine model. Furthermore, physcion downregulated the expression of inflammatory genes induced by tumor necrosis factor-α (TNF-α) in RAW264.7 cells. The underlying mechanism potentially involves the suppression of the NF-κB and MAPK signaling cascades.</p><p><strong>Conclusions: </strong>Our investigation underscores the potential of physcion as a promising candidate for analgesic therapy.</p>","PeriodicalId":9084,"journal":{"name":"Biological Research","volume":"58 1","pages":"48"},"PeriodicalIF":4.3,"publicationDate":"2025-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12243309/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144607307","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Human placenta-derived endothelial progenitor cells: an animal-free culture system for efficient expansion.","authors":"Shengnan Yuan, Mengrou Li, Junhao Wang, Wen Ju, Yujin Huang, Yue Li, Haohan Fan, Lingyu Zeng","doi":"10.1186/s40659-025-00625-2","DOIUrl":"10.1186/s40659-025-00625-2","url":null,"abstract":"<p><strong>Background: </strong>Endothelial progenitor cells (EPCs) play a critical role in vasculogenesis and vascular repair, but their clinical application is hindered by challenges such as cell purity, quantity, and reliance on fetal bovine serum (FBS). This study developed an animal-free system for isolating, induction, and expanding EPCs from the human placenta, evaluating their potential for wound repair.</p><p><strong>Methods: </strong>Mononuclear cells (MNCs) were isolated from full-term placenta and induced into EPCs using an animal-free medium supplemented with bFGF, IGF, and VEGF. EPCs were characterized by flow cytometry for markers CD133, CD34, and VEGFR2, while CD31 and CD45 served as negative markers. Functional assays, including Ac-LDL uptake, migration, and tube formation, confirmed EPC properties. The wound-repair potential was assessed in a mouse model.</p><p><strong>Results: </strong>The induced EPCs exhibited high purity (> 95%) and expressed CD133, CD34, and VEGFR2 while being negative for CD31 and CD45. The system yielded 1 × 10⁸ EPCs from 10 g of placental tissue, demonstrating high proliferative capacity. Functional assays confirmed robust tube formation, migration, and Ac-LDL uptake in vitro. In vivo, EPCs significantly enhanced wound repair.</p><p><strong>Conclusions: </strong>In conclusion, human placenta-derived EPCs cultured in an animal-free system displayed high purity, self-renewal capacity, and functional efficacy, making them a promising cell source for therapeutic applications, particularly in wound repair.</p>","PeriodicalId":9084,"journal":{"name":"Biological Research","volume":"58 1","pages":"44"},"PeriodicalIF":4.3,"publicationDate":"2025-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12219708/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144552144","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}