Biotechnology & Biotechnological Equipment最新文献_第7页

Optimization of intracellular L-Asparaginase production by Streptomyces paulus CA01 isolated from wheat bran using the response surface methodology 利用响应面法优化小麦麸皮paulus链霉菌CA01胞内产l -天冬酰胺酶的条件

IF 1.4 4区生物学

Biotechnology & Biotechnological Equipment Pub Date : 2023-11-29 DOI: 10.1080/13102818.2023.2281493

Achour Chergui, Lamia Trabelsi, Mounir M. Salem-Bekhit, Mohamed H. Mahmoud, Ehab I. Taha, Mohamed A. Ibrahim, Alessandro Erto, Ali Imessaoudene, Mouloud Kecha, Yacine Benguerba, Karim Houali

引用次数: 0

Potential of circulating tumour DNA as a biomarker in liquid biopsy 循环肿瘤DNA作为液体活检生物标志物的潜力

IF 1.4 4区生物学

Biotechnology & Biotechnological Equipment Pub Date : 2023-11-27 DOI: 10.1080/13102818.2023.2287236

Dimitar Dimitrov, Hristo Ivanov, Nelly Miteva-Marcheva, Vili Stoyanova

引用次数: 0

What is the lowest lethal dose of colchicine? 秋水仙碱的最低致死剂量是多少?

IF 1.4 4区生物学

Biotechnology & Biotechnological Equipment Pub Date : 2023-11-27 DOI: 10.1080/13102818.2023.2288240

Vanyo Mitev

引用次数: 0

Analysis of blood microbiome dysbiosis in pulmonary sarcoidosis by decision tree model 决策树模型分析肺结节病血液微生物群落失调

IF 1.4 4区生物学

Biotechnology & Biotechnological Equipment Pub Date : 2023-11-22 DOI: 10.1080/13102818.2023.2283133

Yordan Hodzhev

引用次数: 0

Analyzing real-world data to understand COVID-19 vaccination effects on hospitalization rates in patients with solid malignancies 分析真实世界数据，了解COVID-19疫苗接种对实体恶性肿瘤患者住院率的影响

IF 1.4 4区生物学

Biotechnology & Biotechnological Equipment Pub Date : 2023-11-19 DOI: 10.1080/13102818.2023.2282141

George Dimitrov, Radka Argirova, Trifon Valkov

引用次数: 0

High cross-pollination rate of Greek oregano ( O. vulgare ssp. hirtum ) with Common oregano ( O. vulgare ssp. vulgare) under open field conditions as revealed by microsatellite marker analysis 通过微卫星标记分析发现露地条件下希腊牛至（O. vulgare ssp.

4区生物学

Biotechnology & Biotechnological Equipment Pub Date : 2023-11-03 DOI: 10.1080/13102818.2023.2279636

Marina Evgenieva Alekseeva, Mila Gradeva Rusanova, Liliya Nikolova Georgieva, Krasimir Emilov Rusanov, Ivan Iliev Atanassov

{"title":"High cross-pollination rate of Greek oregano ( O. vulgare ssp. hirtum ) with Common oregano ( O. vulgare ssp. vulgare) under open field conditions as revealed by microsatellite marker analysis","authors":"Marina Evgenieva Alekseeva, Mila Gradeva Rusanova, Liliya Nikolova Georgieva, Krasimir Emilov Rusanov, Ivan Iliev Atanassov","doi":"10.1080/13102818.2023.2279636","DOIUrl":"https://doi.org/10.1080/13102818.2023.2279636","url":null,"abstract":"We studied the mode of pollination in Greek oregano (Origanum vulgare ssp. hirtum) under both controlled and open pollination conditions. When grown indoors without the presence of insects, Greek oregano plants did not develop any seeds, indicating a low level of spontaneous self-pollination. Applying manual self-pollination under the same conditions resulted in only 16 seeds, of which only five were able to germinate. At the same time, a clonally propagated Greek oregano plant of the same genotype produced a rich set of over 300 seeds in open field conditions when the flowers were visited by insects in an area where no other Origanum species were observed. Analysis with SSR markers showed that over 70% of the seeds likely resulted from self-pollination, indicating that insect-mediated pollination is essential for the seed development. We further analyzed the cross-pollination of Greek oregano with Common oregano (O. vulgare ssp. vulgare) in open field conditions where the two subspecies were grown in close proximity. Applying SSR markers, we analyzed 83 plants obtained from seeds of three vegetatively propagated Greek oregano mother plants. Surprisingly, the results showed that all analyzed seedlings resulted from cross-pollination of Greek oregano with Common oregano, indicating that cross-pollination between the two subspecies can completely take over the self-pollination or cross-pollination between the Greek oregano plants. The possible impact of the observed high cross-pollination rate on the genetic origin of seeds of selected Greek oregano lines and varieties, as well as on the genetic diversity and structure of natural populations, is discussed.","PeriodicalId":9076,"journal":{"name":"Biotechnology & Biotechnological Equipment","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-11-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135875271","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bioinformatics analysis and prokaryotic expression of a cystatin analogue from Spirometra erinaceieuropaei 猪肺虫胱抑素类似物的生物信息学分析及原核表达

4区生物学

Biotechnology & Biotechnological Equipment Pub Date : 2023-10-31 DOI: 10.1080/13102818.2023.2277720

Lin Huang, Ling Mai, Gang Lv, Xinjun Chen

{"title":"Bioinformatics analysis and prokaryotic expression of a cystatin analogue from Spirometra erinaceieuropaei","authors":"Lin Huang, Ling Mai, Gang Lv, Xinjun Chen","doi":"10.1080/13102818.2023.2277720","DOIUrl":"https://doi.org/10.1080/13102818.2023.2277720","url":null,"abstract":"Cystatin plays a crucial role in immune evasion by parasites. It regulates diverse immune response processes, such as antigen presentation, cytokine and NO production, and phagocytosis. In recent years, an increasing number of parasite cystatins have been identified and studied for the treatment of inflammatory diseases. In contrast, cystatin from Spirometra erinaceieuropaei has received limited research attention. The objective of this study was to utilize bioinformatics tools and molecular biology techniques to predict the biological properties and obtain a recombinant cystatin analogue from Spirometra erinaceieuropaei (SeCystatin). The SeCystatin gene consists of 417 bp and encodes a putative 100-amino acid protein. The predicted molecular weight and isoelectric point of SeCystatin were 10.89 kDa and 6.82, respectively; SeCystatin possesses 12 phosphorylation sites and five post-translational modification sites but has no signal peptide and transmembrane region. The secondary structure of SeCystatin consists of one α-helix, four β-folds and six coils. It also possesses a cystatin-specific conserved domain, QxVxG, which is positioned at the boundary between the first and second β-folds, thereby demonstrating the characteristic features of type I cystatin. In the context of molecular evolution, SeCystatin demonstrates the highest level of evolutionary similarity to Schistocephalus solidus. The SeCystatin gene was fully synthesized, and the recombinant plasmid pET-30a (+)-SeCystatin was constructed to express the target protein in Escherichia coli BL 21. Recombinant SeCystatin was successfully induced for expression by IPTG and subsequently purified using affinity chromatography (Ni-IDA). Our study provides a solid foundation for further studies on the biological functions of SeCystatin.","PeriodicalId":9076,"journal":{"name":"Biotechnology & Biotechnological Equipment","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135979025","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cost-effectiveness of velmanase alfa vs. bone marrow transplantation or no causal therapy in patients with mild to moderate alpha-mannosidosis velmanase与骨髓移植或无因果治疗在轻度至中度α -甘露甘露病患者中的成本-效果

4区生物学

Biotechnology & Biotechnological Equipment Pub Date : 2023-10-16 DOI: 10.1080/13102818.2023.2271574

Ana Antanasković, Ivana Stević, Refet Gojak, Dragana Lakić, Slobodan Janković

{"title":"Cost-effectiveness of velmanase alfa vs. bone marrow transplantation or no causal therapy in patients with mild to moderate alpha-mannosidosis","authors":"Ana Antanasković, Ivana Stević, Refet Gojak, Dragana Lakić, Slobodan Janković","doi":"10.1080/13102818.2023.2271574","DOIUrl":"https://doi.org/10.1080/13102818.2023.2271574","url":null,"abstract":"Alpha-mannosidosis is an inherited rare disorder of mannose-containing oligosaccharides metabolism that is currently treated by enzyme replacement therapy (ERT), bone marrow transplantation (BMT), or supportive therapy (ST). However, the relative cost-effectiveness of these treatment options is yet unknown. Our study aimed to compare the cost-effectiveness of the treatment options for mild to moderate alpha-mannosidosis. The study is based on a modeling approach using a Discrete-Event Simulation model to generate and simulate the course of the disease under the influence of each of the treatment options: ERT, BMT, and ST. The model had a lifetime horizon and was made from the perspective of the Serbian Health Insurance Fund. Currently, available causal therapy of mild to moderate alpha-mannosidosis with velmanase alpha enzyme replacement is not cost-effective compared with supportive therapy (ICER = 941,587,152 RSD) or bone marrow transplantation (ICER = −398,412,755 RSD). Bone marrow transplantation can be cost-effective compared to supportive therapy (ICER = 6,032,689 RSD), but only if the willingness-to-pay threshold is increased to 9 gross domestic products (GDP) per capita per QALY gained. According to the current threshold, velmanase-alfa is not cost-effective compared to BMT or ST. To make alfa-mannosidosis therapy widely accessible to patients, criteria for assessing the cost-effectiveness of orphan drugs must include not only the absolute value of ICER but other aspects like equity weightings of QALYs, risk-sharing, reimbursement of severe forms of a disease only, or availability of dedicated funding.","PeriodicalId":9076,"journal":{"name":"Biotechnology & Biotechnological Equipment","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136182563","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Traditional microbial control methods used in sake brewing effectively suppress predominant bacteria emerging during production of rice koji 清酒酿造中采用的传统微生物控制方法能有效抑制米曲生产过程中出现的优势菌群

4区生物学

Biotechnology & Biotechnological Equipment Pub Date : 2023-10-14 DOI: 10.1080/13102818.2023.2271566

Kota Naganuma, Youji Nakagawa, Susumu Kokubo, Takuya Hashimoto, Kayo Higuchi, Naoko Ariizumi, Masayuki Hayakawa, Hideki Yamamura

{"title":"Traditional microbial control methods used in sake brewing effectively suppress predominant bacteria emerging during production of rice koji","authors":"Kota Naganuma, Youji Nakagawa, Susumu Kokubo, Takuya Hashimoto, Kayo Higuchi, Naoko Ariizumi, Masayuki Hayakawa, Hideki Yamamura","doi":"10.1080/13102818.2023.2271566","DOIUrl":"https://doi.org/10.1080/13102818.2023.2271566","url":null,"abstract":"Rice koji is a raw material used in the production of sake; however, details regarding the microbial flora and their dynamics in rice koji during the production process are poorly understood. Clarifying these issues can contribute to proposing a method and evaluation that will improve the quality of rice koji and sake. The aim of this study was to determine the microflora in rice koji and the effectiveness of the traditional microbial control techniques used in the sake production process. We analyzed the diversity and changes in bacterial flora during rice koji production by amplicon sequencing of the 16S rRNA gene. The predominant taxon in all rice koji samples was family Staphylococcaceae. The microbial population and the changes in its distribution for five consecutive stages in rice koji production were examined by direct colony counting. Bacteria counts in all samples were below the limit of detection initially, then increased rapidly toward the final stage. The predominant bacterial colonies from all samples were yellow and were identified as Staphylococcus gallinarum through 16S rRNA gene sequence similarity. The S. gallinarum isolates exhibited faster growth in pregelatinized rice medium. Interestingly, the growth of S. gallinarum isolates was suppressed by low temperature (12 °C), ethanol concentration (≥6%) and the addition of lactic acid, which are traditional microbial control methods used during sake fermentation. Therefore, proper control of the traditional sake production process can effectively inhibit the growth of undesirable bacteria such as S. gallinarum that emerge during the production of rice koji.","PeriodicalId":9076,"journal":{"name":"Biotechnology & Biotechnological Equipment","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135804508","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Immunity to mumps virus in children population in Bulgaria 保加利亚儿童对流行性腮腺炎病毒的免疫力

4区生物学

Biotechnology & Biotechnological Equipment Pub Date : 2023-10-13 DOI: 10.1080/13102818.2023.2270606

Stefka Krumova, Radostina Stefanova, Savina Stoitsova, Petia Genova-Kalou, Kremena Parmakova

{"title":"Immunity to mumps virus in children population in Bulgaria","authors":"Stefka Krumova, Radostina Stefanova, Savina Stoitsova, Petia Genova-Kalou, Kremena Parmakova","doi":"10.1080/13102818.2023.2270606","DOIUrl":"https://doi.org/10.1080/13102818.2023.2270606","url":null,"abstract":"Mumps is an acute, contagious, viral vaccine-preventable disease caused by mumps virus (MuV). The measles, mumps, rubella (MMR) vaccine that is used in many countries is considered highly effective with decreased MuV incidence, but suboptimal MuV long-term immunity. This study assessed the MuV seropositivity and antibody titre among vaccinated children in Bulgaria to provide evidence for a better understanding of MuV circulation and immunity in Bulgaria. The samples from 734 immunized children (369 females and 365 males) aged 1 to 16, divided into four age groups (≥1, 2–6, 7–11, and 12–16) were tested. Qualitative and quantitative indirect enzyme-linked immunosorbent assay (Anti-Mumps IgG ELISA, Euroimmun, Germany) was performed to determine the mumps IgG antibody levels in sera. Among all participants, protective MuV immunity was identified in 93%. MuV IgG antibody positivity ranged between 87% in the age group between 1 and 2 years and 96% in the age group 12–16 years, but no statistically significant difference was found among age groups. At the same time, there was a statistically significant difference (p = 0.01) between seropositivity in male and female participants, with male participants having an overall seropositivity of 90% and female 95%. Among the antibody-positive samples, the quantitative measurements of median anti-MuV IgG concentrations showed that titres decreased with increasing age. A slightly waning immunity following vaccination was observed, but positivity remained high among vaccinated children over the years. Similar studies show that maintaining high immunity is crucial to prevent mumps outbreaks.","PeriodicalId":9076,"journal":{"name":"Biotechnology & Biotechnological Equipment","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-10-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135923191","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0