Bone & Joint Research最新文献

{"title":"Diagnosis of periprosthetic joint infections in patients who have rheumatoid arthritis.","authors":"Yulai Wang,&nbsp;Guoqing Li,&nbsp;Baochao Ji,&nbsp;Boyong Xu,&nbsp;Xiaogang Zhang,&nbsp;Asihaerjiang Maimaitiyiming,&nbsp;Li Cao","doi":"10.1302/2046-3758.129.BJR-2022-0432.R1","DOIUrl":"https://doi.org/10.1302/2046-3758.129.BJR-2022-0432.R1","url":null,"abstract":"<p><strong>Aims: </strong>To investigate the optimal thresholds and diagnostic efficacy of commonly used serological and synovial fluid detection indexes for diagnosing periprosthetic joint infection (PJI) in patients who have rheumatoid arthritis (RA).</p><p><strong>Methods: </strong>The data from 348 patients who had RA or osteoarthritis (OA) and had previously undergone a total knee (TKA) and/or a total hip arthroplasty (THA) (including RA-PJI: 60 cases, RA-non-PJI: 80 cases; OA-PJI: 104 cases, OA-non-PJI: 104 cases) were retrospectively analyzed. A receiver operating characteristic curve was used to determine the optimal thresholds of the CRP, ESR, synovial fluid white blood cell count (WBC), and polymorphonuclear neutrophil percentage (PMN%) for diagnosing RA-PJI and OA-PJI. The diagnostic efficacy was evaluated by comparing the area under the curve (AUC) of each index and applying the results of the combined index diagnostic test.</p><p><strong>Results: </strong>For PJI prediction, the results of serological and synovial fluid indexes were different between the RA-PJI and OA-PJI groups. The optimal cutoff value of CRP for diagnosing RA-PJI was 12.5 mg/l, ESR was 39 mm/hour, synovial fluid WBC was 3,654/μl, and PMN% was 65.9%; and those of OA-PJI were 8.2 mg/l, 31 mm/hour, 2,673/μl, and 62.0%, respectively. In the RA-PJI group, the specificity (94.4%), positive predictive value (97.1%), and AUC (0.916) of synovial fluid WBC were higher than those of the other indexes. The optimal cutoff values of synovial fluid WBC and PMN% for diagnosing RA-PJI after THA were significantly higher than those of TKA. The specificity and positive predictive value of the combined index were 100%.</p><p><strong>Conclusion: </strong>Serum inflammatory and synovial fluid indexes can be used for diagnosing RA-PJI, for which synovial fluid WBC is the best detection index. Combining multiple detection indexes can provide a reference basis for the early and accurate diagnosis of RA-PJI.</p>","PeriodicalId":9074,"journal":{"name":"Bone & Joint Research","volume":"12 9","pages":"559-570"},"PeriodicalIF":4.6,"publicationDate":"2023-09-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/1a/b4/BJR-12-2046-3758.129.BJR-2022-0432.R1.PMC10499527.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10259038","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Treatment of infected bone defects with the induced membrane technique.","authors":"Jie Shen, Zhiyuan Wei, Shulin Wang, Xiaohua Wang, Wei Lin, Lei Liu, Guanglin Wang","doi":"10.1302/2046-3758.129.BJR-2022-0439.R2","DOIUrl":"10.1302/2046-3758.129.BJR-2022-0439.R2","url":null,"abstract":"<p><strong>Aims: </strong>This study aimed to evaluate the effectiveness of the induced membrane technique for treating infected bone defects, and to explore the factors that might affect patient outcomes.</p><p><strong>Methods: </strong>A comprehensive search was performed in PubMed, Embase, and the Cochrane Central Register of Controlled Trials databases between 1 January 2000 and 31 October 2021. Studies with a minimum sample size of five patients with infected bone defects treated with the induced membrane technique were included. Factors associated with nonunion, infection recurrence, and additional procedures were identified using logistic regression analysis on individual patient data.</p><p><strong>Results: </strong>After the screening, 44 studies were included with 1,079 patients and 1,083 segments of infected bone defects treated with the induced membrane technique. The mean defect size was 6.8 cm (0.5 to 30). After the index second stage procedure, 85% (797/942) of segments achieved union, and 92% (999/1,083) of segments achieved final healing. The multivariate analysis with data from 296 patients suggested that older age was associated with higher nonunion risk. Patients with external fixation in the second stage had a significantly higher risk of developing nonunion, increasing the need for additional procedures. The autografts harvested from the femur reamer-irrigator-aspirator increased nonunion, infection recurrence, and additional procedure rates.</p><p><strong>Conclusion: </strong>The induced membrane technique is an effective technique for treating infected bone defects. Internal fixation during the second stage might effectively promote bone healing and reduce additional procedures without increasing infection recurrence. Future studies should standardize individual patient data prospectively to facilitate research on the affected patient outcomes.</p>","PeriodicalId":9074,"journal":{"name":"Bone & Joint Research","volume":"12 9","pages":"546-558"},"PeriodicalIF":4.7,"publicationDate":"2023-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/76/b7/BJR-12-2046-3758.129.BJR-2022-0439.R2.PMC10495849.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10241862","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The role of cells and signal pathways in subchondral bone in osteoarthritis.","authors":"Pan Luo,&nbsp;Qi-Ling Yuan,&nbsp;Mingyi Yang,&nbsp;Xianjie Wan,&nbsp;Peng Xu","doi":"10.1302/2046-3758.129.BJR-2023-0081.R1","DOIUrl":"https://doi.org/10.1302/2046-3758.129.BJR-2023-0081.R1","url":null,"abstract":"<p><p>Osteoarthritis (OA) is mainly caused by ageing, strain, trauma, and congenital joint abnormalities, resulting in articular cartilage degeneration. During the pathogenesis of OA, the changes in subchondral bone (SB) are not only secondary manifestations of OA, but also an active part of the disease, and are closely associated with the severity of OA. In different stages of OA, there were microstructural changes in SB. Osteocytes, osteoblasts, and osteoclasts in SB are important in the pathogenesis of OA. The signal transduction mechanism in SB is necessary to maintain the balance of a stable phenotype, extracellular matrix (ECM) synthesis, and bone remodelling between articular cartilage and SB. An imbalance in signal transduction can lead to reduced cartilage quality and SB thickening, which leads to the progression of OA. By understanding changes in SB in OA, researchers are exploring drugs that can regulate these changes, which will help to provide new ideas for the treatment of OA.</p>","PeriodicalId":9074,"journal":{"name":"Bone & Joint Research","volume":"12 9","pages":"536-545"},"PeriodicalIF":4.6,"publicationDate":"2023-09-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/15/6f/BJR-12-2046-3758.129.BJR-2023-0081.R1.PMC10484649.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10190474","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification and experimental validation of key extracellular proteins as potential targets in intervertebral disc degeneration.","authors":"Guang-Zhi Zhang,&nbsp;Lei Li,&nbsp;Zhang-Bin Luo,&nbsp;Cang-Yu Zhang,&nbsp;Yong-Gang Wang,&nbsp;Xue-Wen Kang","doi":"10.1302/2046-3758.129.BJR-2022-0369.R2","DOIUrl":"https://doi.org/10.1302/2046-3758.129.BJR-2022-0369.R2","url":null,"abstract":"<p><strong>Aims: </strong>This study aimed, through bioinformatics analysis and in vitro experiment validation, to identify the key extracellular proteins of intervertebral disc degeneration (IDD).</p><p><strong>Methods: </strong>The gene expression profile of GSE23130 was downloaded from the Gene Expression Omnibus (GEO) database. Extracellular protein-differentially expressed genes (EP-DEGs) were screened by protein annotation databases, and we used Gene Ontology (GO) and the Kyoto Encyclopedia of Genes and Genomes (KEGG) to analyze the functions and pathways of EP-DEGs. STRING and Cytoscape were used to construct protein-protein interaction (PPI) networks and identify hub EP-DEGs. NetworkAnalyst was used to analyze transcription factors (TFs) and microRNAs (miRNAs) that regulate hub EP-DEGs. A search of the Drug Signatures Database (DSigDB) for hub EP-DEGs revealed multiple drug molecules and drug-target interactions.</p><p><strong>Results: </strong>A total of 56 EP-DEGs were identified in the differential expression analysis. EP-DEGs were enriched in the extracellular structure organization, ageing, collagen-activated signalling pathway, PI3K-Akt signalling pathway, and AGE-RAGE signalling pathway. PPI network analysis showed that the top ten hub EP-DEGs are closely related to IDD. Correlation analysis also demonstrated a significant correlation between the ten hub EP-DEGs (p＜0.05), which were selected to construct TF-gene interaction and TF-miRNA coregulatory networks. In addition, ten candidate drugs were screened for the treatment of IDD.</p><p><strong>Conclusion: </strong>The findings clarify the roles of extracellular proteins in IDD and highlight their potential as promising novel therapeutic targets.</p>","PeriodicalId":9074,"journal":{"name":"Bone & Joint Research","volume":"12 9","pages":"522-535"},"PeriodicalIF":4.6,"publicationDate":"2023-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/19/bb/BJR-12-2046-3758.129.BJR-2022-0369.R2.PMC10475329.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10161458","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Predicting whether patients will achieve minimal clinically important differences following hip or knee arthroplasty.","authors":"Benedikt Langenberger,&nbsp;Daniel Schrednitzki,&nbsp;Andreas M Halder,&nbsp;Reinhard Busse,&nbsp;Christoph M Pross","doi":"10.1302/2046-3758.129.BJR-2023-0070.R2","DOIUrl":"https://doi.org/10.1302/2046-3758.129.BJR-2023-0070.R2","url":null,"abstract":"<p><strong>Aims: </strong>A substantial fraction of patients undergoing knee arthroplasty (KA) or hip arthroplasty (HA) do not achieve an improvement as high as the minimal clinically important difference (MCID), i.e. do not achieve a meaningful improvement. Using three patient-reported outcome measures (PROMs), our aim was: 1) to assess machine learning (ML), the simple pre-surgery PROM score, and logistic-regression (LR)-derived performance in their prediction of whether patients undergoing HA or KA achieve an improvement as high or higher than a calculated MCID; and 2) to test whether ML is able to outperform LR or pre-surgery PROM scores in predictive performance.</p><p><strong>Methods: </strong>MCIDs were derived using the change difference method in a sample of 1,843 HA and 1,546 KA patients. An artificial neural network, a gradient boosting machine, least absolute shrinkage and selection operator (LASSO) regression, ridge regression, elastic net, random forest, LR, and pre-surgery PROM scores were applied to predict MCID for the following PROMs: EuroQol five-dimension, five-level questionnaire (EQ-5D-5L), EQ visual analogue scale (EQ-VAS), Hip disability and Osteoarthritis Outcome Score-Physical Function Short-form (HOOS-PS), and Knee injury and Osteoarthritis Outcome Score-Physical Function Short-form (KOOS-PS).</p><p><strong>Results: </strong>Predictive performance of the best models per outcome ranged from 0.71 for HOOS-PS to 0.84 for EQ-VAS (HA sample). ML statistically significantly outperformed LR and pre-surgery PROM scores in two out of six cases.</p><p><strong>Conclusion: </strong>MCIDs can be predicted with reasonable performance. ML was able to outperform traditional methods, although only in a minority of cases.</p>","PeriodicalId":9074,"journal":{"name":"Bone & Joint Research","volume":"12 9","pages":"512-521"},"PeriodicalIF":4.6,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/2d/a8/BJR-12-2046-3758.129.BJR-2023-0070.R2.PMC10471446.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10160946","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Thickness of simple calcaneal tuberosity avulsion fractures influences the optimal fixation method employed.","authors":"Chunliang Wang,&nbsp;Shih-Jung Liu,&nbsp;Chung-Hsun Chang","doi":"10.1302/2046-3758.128.BJR-2023-0060.R1","DOIUrl":"https://doi.org/10.1302/2046-3758.128.BJR-2023-0060.R1","url":null,"abstract":"<p><strong>Aims: </strong>This study aimed to establish the optimal fixation methods for calcaneal tuberosity avulsion fractures with different fragment thicknesses in a porcine model.</p><p><strong>Methods: </strong>A total of 36 porcine calcanea were sawed to create simple avulsion fractures with three different fragment thicknesses (5, 10, and 15 mm). They were randomly fixed with either two suture anchors or one headless screw. Load-to-failure and cyclic loading tension tests were performed for the biomechanical analysis.</p><p><strong>Results: </strong>This biomechanical study predicts that headless screw fixation is a better option if fragment thickness is over 15 mm in terms of the comparable peak failure load to suture anchor fixation (headless screw: 432.55 N (SD 62.25); suture anchor: 446.58 N (SD 84.97)), and less fracture fragment displacement after cyclic loading (headless screw: 3.94 N (SD 1.76); suture anchor: 8.68 N (SD 1.84)). Given that the fragment thickness is less than 10 mm, suture anchor fixation is a safer option.</p><p><strong>Conclusion: </strong>Fracture fragment thickness helps in making the decision of either using headless screw or suture anchor fixation in treating calcaneal tuberosity avulsion fracture, based on the regression models of our study.</p>","PeriodicalId":9074,"journal":{"name":"Bone & Joint Research","volume":"12 8","pages":"504-511"},"PeriodicalIF":4.6,"publicationDate":"2023-08-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/14/fa/BJR-12-2046-3758.128.BJR-2023-0060.R1.PMC10444534.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10056181","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of surface matching mismatch of focal knee articular prosthetic on tibiofemoral contact stress using finite element analysis.","authors":"Jin-Ah Lee,&nbsp;Yong-Gon Koh,&nbsp;Paul S Kim,&nbsp;Joon-Hee Park,&nbsp;Kyoung-Tak Kang","doi":"10.1302/2046-3758.128.BJR-2023-0010.R1","DOIUrl":"https://doi.org/10.1302/2046-3758.128.BJR-2023-0010.R1","url":null,"abstract":"<p><strong>Aims: </strong>Focal knee arthroplasty is an attractive alternative to knee arthroplasty for young patients because it allows preservation of a large amount of bone for potential revisions. However, the mechanical behaviour of cartilage has not yet been investigated because it is challenging to evaluate in vivo contact areas, pressure, and deformations from metal implants. Therefore, this study aimed to determine the contact pressure in the tibiofemoral joint with a focal knee arthroplasty using a finite element model.</p><p><strong>Methods: </strong>The mechanical behaviour of the cartilage surrounding a metal implant was evaluated using finite element analysis. We modelled focal knee arthroplasty with placement flush, 0.5 mm deep, or protruding 0.5 mm with regard to the level of the surrounding cartilage. We compared contact stress and pressure for bone, implant, and cartilage under static loading conditions.</p><p><strong>Results: </strong>Contact stress on medial and lateral femoral and tibial cartilages increased and decreased, respectively, the most and the least in the protruding model compared to the intact model. The deep model exhibited the closest tibiofemoral contact stress to the intact model. In addition, the deep model demonstrated load sharing between the bone and the implant, while the protruding and flush model showed stress shielding. The data revealed that resurfacing with a focal knee arthroplasty does not cause increased contact pressure with deep implantation. However, protruding implantation leads to increased contact pressure, decreased bone stress, and biomechanical disadvantage in an in vivo application.</p><p><strong>Conclusion: </strong>These results show that it is preferable to leave an edge slightly deep rather than flush and protruding.</p>","PeriodicalId":9074,"journal":{"name":"Bone & Joint Research","volume":"12 8","pages":"497-503"},"PeriodicalIF":4.6,"publicationDate":"2023-08-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/e6/2b/BJR-12-2046-3758.128.BJR-2023-0010.R1.PMC10427223.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10037139","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Artificial intelligence in orthopaedics.","authors":"Nick D Clement,&nbsp;A H R W Simpson","doi":"10.1302/2046-3758.128.BJR-2023-0199","DOIUrl":"https://doi.org/10.1302/2046-3758.128.BJR-2023-0199","url":null,"abstract":"Cite this article: Bone Joint Res 2023;12(8):494–496.","PeriodicalId":9074,"journal":{"name":"Bone & Joint Research","volume":"12 8","pages":"494-496"},"PeriodicalIF":4.6,"publicationDate":"2023-08-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/50/0c/BJR-12-2046-3758.128.BJR-2023-0199.PMC10409576.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9969768","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Adiponectin inhibits fibrosis of the palmar aponeurosis in Dupuytren's contracture in male patients.","authors":"Yoshiaki Yamanaka,&nbsp;Takafumi Tajima,&nbsp;Yoshitaka Tsujimura,&nbsp;Toichiro Naito,&nbsp;Yosuke Mano,&nbsp;Manabu Tsukamoto,&nbsp;Yukichi Zenke,&nbsp;Akinori Sakai","doi":"10.1302/2046-3758.128.BJR-2022-0449.R1","DOIUrl":"https://doi.org/10.1302/2046-3758.128.BJR-2022-0449.R1","url":null,"abstract":"<p><strong>Aims: </strong>Dupuytren's contracture is characterized by increased fibrosis of the palmar aponeurosis, with eventual replacement of the surrounding fatty tissue with palmar fascial fibromatosis. We hypothesized that adipocytokines produced by adipose tissue in contact with the palmar aponeurosis might promote fibrosis of the palmar aponeurosis.</p><p><strong>Methods: </strong>We compared the expression of the adipocytokines adiponectin and leptin in the adipose tissue surrounding the palmar aponeurosis of male patients with Dupuytren's contracture, and of male patients with carpal tunnel syndrome (CTS) as the control group. We also examined the effects of adiponectin on fibrosis-related genes and proteins expressed by fibroblasts in the palmar aponeurosis of patients with Dupuytren's contracture.</p><p><strong>Results: </strong>Adiponectin expression in the adipose tissue surrounding the palmar aponeurosis was significantly lower in patients with Dupuytren's contracture than in those with CTS. The expression of fibrosis-related genes and proteins, such as types 1 and 3 collagen and α-smooth muscle actin, was suppressed in a concentration-dependent manner by adding AdipoRon, an adiponectin receptor agonist. The expression of fibrosis-related genes and proteins was also suppressed by AdipoRon in the in vitro model of Dupuytren's contracture created by adding TGF-β to normal fibroblasts collected from patients with CTS.</p><p><strong>Conclusion: </strong>Fibrosis of the palmar aponeurosis in Dupuytren's contracture in males may be associated with adiponectin expression in the adipose tissue surrounding the palmar aponeurosis. Although fibroblasts within the palmar aponeurosis are often the focus of attention when elucidating the pathogenesis of Dupuytren's contracture, adiponectin expression in adipose tissues warrants closer attention in future research.</p>","PeriodicalId":9074,"journal":{"name":"Bone & Joint Research","volume":"12 8","pages":"486-493"},"PeriodicalIF":4.6,"publicationDate":"2023-08-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/2b/d0/BJR-12-2046-3758.128.BJR-2022-0449.R1.PMC10400293.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9937978","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Astragalus polysaccharide promotes osteogenic differentiation of human bone marrow derived mesenchymal stem cells by facilitating ANKFY1 expression through miR-760 inhibition.","authors":"Xianfeng Hu,&nbsp;Liu Yang,&nbsp;Yanhua Du,&nbsp;Xiangping Meng,&nbsp;Yuanyuan Shi,&nbsp;Juan Zeng","doi":"10.1302/2046-3758.128.BJR-2022-0248.R2","DOIUrl":"https://doi.org/10.1302/2046-3758.128.BJR-2022-0248.R2","url":null,"abstract":"<p><strong>Aims: </strong>Astragalus polysaccharide (APS) participates in various processes, such as the enhancement of immunity and inhibition of tumours. APS can affect osteoporosis (OP) by regulating the osteogenic differentiation of human bone mesenchymal stem cells (hBMSCs). This study was designed to elucidate the mechanism of APS in hBMSC proliferation and osteoblast differentiation.</p><p><strong>Methods: </strong>Reverse transcriptase polymerase chain reaction (RT-PCR) and Western blotting were performed to determine the expression of microRNA (miR)-760 and ankyrin repeat and FYVE domain containing 1 (ANKFY1) in OP tissues and hBMSCs. Cell viability was measured using the Cell Counting Kit-8 assay. The expression of cyclin D1 and osteogenic marker genes (osteocalcin (OCN), alkaline phosphatase (ALP), and runt-related transcription factor 2 (RUNX2)) was evaluated using quantitative reverse transcriptase polymerase chain reaction (qRT-PCR). Mineral deposits were detected through Alizarin Red S staining. In addition, Western blotting was performed to detect the ANKFY1 protein levels following the regulation of miR-760. The relationship between miR-760 and ANKFY1 was determined using a luciferase reporter assay.</p><p><strong>Results: </strong>The expression of miR-760 was upregulated in OP tissues, whereas ANKFY1 expression was downregulated. APS stimulated the differentiation and proliferation of hBMSCs by: increasing their viability; upregulating the expression levels of cyclin D1, ALP, OCN, and RUNX2; and inducing osteoblast mineralization. Moreover, APS downregulated the expression of miR-760. Overexpression of miR-760 was found to inhibit the promotive effect of APS on hBMSC differentiation and proliferation, while knockdown of miR-760 had the opposite effect. ANKFY1 was found to be the direct target of miR-760. Additionally, ANKFY1 participated in the APS-mediated regulation of miR-760 function in hBMSCs.</p><p><strong>Conclusion: </strong>APS promotes the osteogenic differentiation and proliferation of hBMSCs. Moreover, APS alleviates the effects of OP by downregulating miR-760 and upregulating ANKFY1 expression.</p>","PeriodicalId":9074,"journal":{"name":"Bone & Joint Research","volume":"12 8","pages":"476-485"},"PeriodicalIF":4.6,"publicationDate":"2023-08-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/e6/1d/BJR-12-2046-3758.128.BJR-2022-0248.R2.PMC10396440.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10295077","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}