Detection of rare microorganisms in bone and joint infections by metagenomic next-generation sequencing.

IF 4.7 2区 医学 Q2 CELL & TISSUE ENGINEERING
Hongxin Hu, Haiqi Ding, Jianhua Lyu, Yang Chen, Changyu Huang, Chaofan Zhang, Wenbo Li, Xinyu Fang, Wenming Zhang
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引用次数: 0

Abstract

Aims: This aim of this study was to analyze the detection rate of rare pathogens in bone and joint infections (BJIs) using metagenomic next-generation sequencing (mNGS), and the impact of mNGS on clinical diagnosis and treatment.

Methods: A retrospective analysis was conducted on 235 patients with BJIs who were treated at our hospital between January 2015 and December 2021. Patients were divided into the no-mNGS group (microbial culture only) and the mNGS group (mNGS testing and microbial culture) based on whether mNGS testing was used or not.

Results: A total of 147 patients were included in the no-mNGS group and 88 in the mNGS group. The mNGS group had a higher detection rate of rare pathogens than the no-mNGS group (21.6% vs 10.2%, p = 0.016). However, the mNGS group had lower rates of antibiotic-related complications, shorter hospital stays, and higher infection control rates compared with the no-mNGS group (p = 0.017, p = 0.003, and p = 0.028, respectively), while there was no significant difference in the duration of antibiotic use (p = 0.957). In culture-negative cases, the mNGS group had lower rates of antibiotic-related complications, shorter hospital stays, and a higher infection control rate than the no-mNGS group (p = 0.036, p = 0.033, p = 0.022, respectively), while there was no significant difference in the duration of antibiotic use (p = 0.748).

Conclusion: mNGS improves detection of rare pathogens in BJIs. mNGS testing reduces antibiotic-related complications, shortens hospital stay and antibiotic use duration, and improves treatment success rate, benefits which are particularly evident in culture-negative cases.

通过元基因组新一代测序检测骨关节感染中的罕见微生物。
目的:本研究旨在利用元基因组新一代测序技术(mNGS)分析骨与关节感染(BJIs)中罕见病原体的检出率,以及mNGS对临床诊断和治疗的影响:对 2015 年 1 月至 2021 年 12 月期间在我院接受治疗的 235 例 BJI 患者进行了回顾性分析。根据是否使用 mNGS 检测,将患者分为无 mNGS 组(仅微生物培养)和 mNGS 组(mNGS 检测和微生物培养):无 mNGS 组共纳入 147 名患者,mNGS 组共纳入 88 名患者。mNGS 组的罕见病原体检出率高于无 mNGS 组(21.6% vs 10.2%,p = 0.016)。然而,与无 mNGS 组相比,mNGS 组的抗生素相关并发症发生率更低、住院时间更短、感染控制率更高(分别为 p = 0.017、p = 0.003 和 p = 0.028),而抗生素使用时间则无显著差异(p = 0.957)。在培养阴性病例中,与无 mNGS 组相比,mNGS 组的抗生素相关并发症发生率更低、住院时间更短、感染控制率更高(分别为 p = 0.036、p = 0.033、p = 0.022),而抗生素使用持续时间无明显差异(p = 0.748)。mNGS 检测可减少抗生素相关并发症,缩短住院时间和抗生素使用时间,提高治疗成功率,这些优势在培养阴性病例中尤为明显。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Bone & Joint Research
Bone & Joint Research CELL & TISSUE ENGINEERING-ORTHOPEDICS
CiteScore
7.40
自引率
23.90%
发文量
156
审稿时长
12 weeks
期刊介绍: The gold open access journal for the musculoskeletal sciences. Included in PubMed and available in PubMed Central.
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