Journal of mammalian ova research最新文献_第7页

Preimplantation Diagnosis of Duchenne Muscular Dystrophy 杜氏肌营养不良症的植入前诊断

Journal of mammalian ova research Pub Date : 2004-01-01 DOI: 10.1274/JMOR.21.7

T. Hashiba, Hiroyoshi Watanabe, T. Maeda, Hiroto Tajima, N. Kuji, K. Sueoka, Y. Yoshimura

引用次数: 0

Time Dependent Changes in Progesterone Receptor Expression in Cumulus Cells During Meiotic Resumption of Porcine Oocytes 猪卵母细胞减数分裂恢复过程中卵丘细胞孕酮受体表达的时间依赖性变化

Journal of mammalian ova research Pub Date : 2003-10-01 DOI: 10.1274/JMOR.20.113

M. Shimada, M. Nishibori

{"title":"Time Dependent Changes in Progesterone Receptor Expression in Cumulus Cells During Meiotic Resumption of Porcine Oocytes","authors":"M. Shimada, M. Nishibori","doi":"10.1274/JMOR.20.113","DOIUrl":"https://doi.org/10.1274/JMOR.20.113","url":null,"abstract":"In this study, to investigate the time dependent changes in progesterone receptor (PR) expression in cumulus cells during meiotic resumption of porcine oocytes, each amount of PR-A and PR-B mRNA was analyzed by RT-PCR with primer sets for the PR-B region and the PR-A/B common region. The results showed that the levels of both PR-A/B and PR-B mRNA were very low in cumulus cells immediately recovered from their follicles. The cultivation with FSH and LH significantly increased the level of both PR-A/B and PR- B mRNA in cumulus cell of COCs, whereas the level of PR-B mRNA significantly decreased at 12-hr cultivation. Nevertheless, the higher level of PR-A/B mRNA was maintained up to 20-hr cultivation, suggesting that PR-A was mainly expressed in cumulus cells during cultivation from 12 hr to 20 hr. When COCs were cultured for 10 hr and then further cultured with RU486 for 10 hr, the proportion of oocytes undergoing GVBD significantly decreased in a dose dependent fashion. These results suggest that the high ratios of PR-A to PR-B in cumulus cells of COCs during 12-hr to 20-hr cultivation, are required for meiotic resumption of porcine cumulus-","PeriodicalId":90599,"journal":{"name":"Journal of mammalian ova research","volume":"30 1","pages":"113-117"},"PeriodicalIF":0.0,"publicationDate":"2003-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81371633","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Oocyte Maturation in Humans: the Potential Relevance to Reproductive Medicine 人类卵母细胞成熟:与生殖医学的潜在关联

Journal of mammalian ova research Pub Date : 2003-10-01 DOI: 10.1274/JMOR.20.86

Y. Yoshimura

{"title":"Oocyte Maturation in Humans: the Potential Relevance to Reproductive Medicine","authors":"Y. Yoshimura","doi":"10.1274/JMOR.20.86","DOIUrl":"https://doi.org/10.1274/JMOR.20.86","url":null,"abstract":"Mammalian oogenesis is characterized by alternating periods of active meiotic progression and intermittent, long periods of meiotic arrest. The oocyte undergoes major growth and developmental processes in the period prior to ovulation. At the time of birth, most oocytes are arrested in the dictyate stage of meiosis; they remain quiescent for an indeterminate period until they begin growing in response to as yet undefined local signals [1]. From the beginning of the growth phase until the time of ovulation, oocytes increase in volume by at least two orders of magnitude and during this time exhibit intense metabolic activity. Resumption of meiosis only occurs in a ful ly grown, meiotically competent oocyte after the luteinizing hormone (LH) surge when oocytes undergo germina l ves ic le breakdown (GVBD), complete first meiosis, and mature to metaphase II. In many mammals such as the mouse, pig, cow and man, completion of meiosis is dependent on fertilization that triggers progression to anaphase II, and to the first mitotic interphase with formation of female and male pronuclei. The process of maturation encompasses a complex series of molecular and structural events, culminating in the arrest of the oocyte chromosomes on the metaphase II plate in anticipation of sperm penetration and activation for fertilization. This review will focus on the biology of oocyte maturation and the potential relevance of maturation of human oocytes in vitro to reproductive medicine.","PeriodicalId":90599,"journal":{"name":"Journal of mammalian ova research","volume":"40 1","pages":"86-92"},"PeriodicalIF":0.0,"publicationDate":"2003-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79611452","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Localization and Function of Cyclin B1 and Cyclin B2 during Porcine Oocyte Maturation 猪卵母细胞成熟过程中细胞周期蛋白B1和B2的定位和功能

Journal of mammalian ova research Pub Date : 2003-10-01 DOI: 10.1274/JMOR.20.93

Takao Kuroda, K. Naito

{"title":"Localization and Function of Cyclin B1 and Cyclin B2 during Porcine Oocyte Maturation","authors":"Takao Kuroda, K. Naito","doi":"10.1274/JMOR.20.93","DOIUrl":"https://doi.org/10.1274/JMOR.20.93","url":null,"abstract":"Oocyte maturation is regulated by maturation/M-phase promoting factor (MPF), a crucial M-phase regulating enzyme composed of a catalytic subunit, p34 cdc2 , and a regulatory subunit, cyclin B. The amount of p34 cdc2 is almost constant during oocyte maturation, and the amount of cyclin B is the principal factor determining MPF activity [1]. The presence of two types of cyclin B, cyclin B1 and cyclin B2, has been shown in vertebrates. In human cells, cyclin B1 can cause chromosome condensation, reorganization of the microtubules, and disassembly of the nuclear lamina and of the Golgi apparatus, whereas the role of cyclin B2 is restricted only to disassembly of the Golgi apparatus [2, 3]. In maturing oocytes, differences between cyclin B1 and cyclin B2 functions have been reported in the first meiotic spindle formation and the second metaphase arrest in frog and mouse oocytes, respectively [4(cid:150)6]. In our laboratory, we have studied cyclin B functions during porcine oocyte maturation for the past several years. The present review describes our recent observations with regard to protein levels, intracellular localizations and roles of cyclin B. We focus here on the differences between cyclin B1 and cyclin B2. density of","PeriodicalId":90599,"journal":{"name":"Journal of mammalian ova research","volume":"28 1","pages":"93-98"},"PeriodicalIF":0.0,"publicationDate":"2003-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77590300","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

Specific Inhibition of Transient and Stable EGFP Gene Expression by Double Stranded RNA Interference in Mouse Preimplantation Embryos 双链RNA干扰对小鼠着床前胚胎瞬时和稳定EGFP基因表达的特异性抑制

Journal of mammalian ova research Pub Date : 2003-10-01 DOI: 10.1274/JMOR.20.99

M. Hosoe, T. Furusawa, F. Inoue, M. Sakatani, T. Tokunaga, R. Schultz, Masashi Takahashi

{"title":"Specific Inhibition of Transient and Stable EGFP Gene Expression by Double Stranded RNA Interference in Mouse Preimplantation Embryos","authors":"M. Hosoe, T. Furusawa, F. Inoue, M. Sakatani, T. Tokunaga, R. Schultz, Masashi Takahashi","doi":"10.1274/JMOR.20.99","DOIUrl":"https://doi.org/10.1274/JMOR.20.99","url":null,"abstract":"Double stranded RNA (dsRNA) interference is a useful tool for interfering with gene function by promoting the sequence-dependent degradation of targeted mRNA in several organisms. In the present study, in order to confirm and improve the effect of dsRNA, we investigated an inhibitory effect of dsRNA on both transient and stable gene expression of enhanced green fluorescent protein (EGFP) in mouse preimplantation embryos. In the transient expression system, the rates of fluorescent embryos were significantly decreased by co-injection of EGFP dsRNA and EGFP expression vector fragment into the pronucleus of zygotes. In the stable expression system, EGFP expression in transgenic embryos was significantly decreased by injection of EGFP dsRNA into both the pronucleus and cytoplasm of zygotes, but, cytoplasmic injection caused a more significant EGFP inhibition than pronuclear injection. In quantitative PCR analysis, the expression of the EGFP gene was also inhibited by dsRNA injection, whereas the endogenous gene expression was not affected. These data suggest that dsRNA can inhibit the specific gene expression without affecting the development and expression of other genes.","PeriodicalId":90599,"journal":{"name":"Journal of mammalian ova research","volume":"42 1","pages":"99-105"},"PeriodicalIF":0.0,"publicationDate":"2003-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80794644","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

In Vitro Growth of Oocytes from Domestic Species 国产种卵母细胞的体外培养

Journal of mammalian ova research Pub Date : 2003-10-01 DOI: 10.1274/JMOR.20.78

T. Miyano, Y. Hirao

引用次数: 18

A Vitrification Method by Means of a Straw to Prevent Infections in Mouse Pronuclear Embryos 用吸管玻璃化法预防小鼠原核胚胎感染

Journal of mammalian ova research Pub Date : 2003-10-01 DOI: 10.1274/JMOR.20.124

M. Kumon, Y. Kumasako, T. Utsunomiya, Y. Araki

引用次数: 4

Germ Cell Differentiation in Culture 培养中的生殖细胞分化

Journal of mammalian ova research Pub Date : 2003-10-01 DOI: 10.1274/JMOR.20.69

T. Noce

{"title":"Germ Cell Differentiation in Culture","authors":"T. Noce","doi":"10.1274/JMOR.20.69","DOIUrl":"https://doi.org/10.1274/JMOR.20.69","url":null,"abstract":"The generation of animals cloned by means of the nuclear transfer technique indicates that somatic cell nuc le i can be rep rogrammed when t hey a re transplanted into the egg cytoplasmin other words, the transplanted somatic nuclei can be induced to return to a pluripotent state similar to that of the nuclei of fert il ized eggs. Therefore, the potential for both reprogramming and pluripotency is retained in germ cells throughout their development. For example, embryonic germ (EG) cells that are generated from primordial germ cells (PGCs) show similar pluripotency to the undifferentiated embryonic stem (ES) cells that originate in the inner cell mass of blastocyst embryos in mice [1 ] . And some te ra tomas tha t genera te differentiated cells of various typesfrom all three pr imary germ layersor ig inate in PGCs in the embryonic gonads [2]. Therefore, germ cells must have the ab i l i t y to be reprogrammed, as we l l as to differentiate to form gametes. So how do germ cells acquire these character ist ics, and how are they maintained during development? The discovery of the molecular and cellular mechanisms that are involved in these processes will provide important insights not only for basic research into reproductive biology but also for the development of new techniques for regenerative medical treatments. There is an urgent need to develop i n v i t r o s ys tems t ha t can mode l t he en t i r e developmental processfrom pluripotent stem cells to func t i ona l spe rm and eggsto f u r t he r ou r understanding of the mechanisms of germ-cel l differentiation.","PeriodicalId":90599,"journal":{"name":"Journal of mammalian ova research","volume":"32 1","pages":"69-73"},"PeriodicalIF":0.0,"publicationDate":"2003-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84226112","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Golgi-Associated PDZ and Coiled-Coil Motif-Containing Protein 高尔基相关PDZ和盘绕盘绕基序蛋白

Journal of mammalian ova research Pub Date : 2003-08-01 DOI: 10.32388/uqy6ze

呉竹昭治, 八尾良司, 柳田薫, 佐藤章, 年森清隆, 野田哲生

引用次数: 0

Trials for Improvement of Blastocyst Stage Transfer Technique 囊胚期移植技术改进的试验

Journal of mammalian ova research Pub Date : 2003-04-01 DOI: 10.1274/JMOR.20.20

Y. Morimoto, Takuji Nishihara

引用次数: 0