Journal of mammalian ova research最新文献_第7页

Production of Calves Derived from Embryos Produced by In Vitro Matured, Fertilized and Cultured Oocytes Recovered from Individual Cows 用母牛个体的体外成熟、受精和培养卵母细胞产生的胚胎生产小牛

Journal of mammalian ova research Pub Date : 2004-04-01 DOI: 10.1274/JMOR.21.52

S. Abe, T. Okazaki, T. Shiraishi

{"title":"Production of Calves Derived from Embryos Produced by In Vitro Matured, Fertilized and Cultured Oocytes Recovered from Individual Cows","authors":"S. Abe, T. Okazaki, T. Shiraishi","doi":"10.1274/JMOR.21.52","DOIUrl":"https://doi.org/10.1274/JMOR.21.52","url":null,"abstract":"The objective of this study was to improve culture condition of in vitro produced bovine embryo cultured in CR1aa and to examine the developmental ability of the oocytes from a small number of oocytes per drop culture to blastocyst stage in vitro. Bovine cumulus-oocytes complexes (COCs) were matured, fertilized in a 100 μl-drop (10 presumptive COCs/drop) of medium. After insemination, zygotes were divided into three groups; (1) CR1aa: zygotes were cultured in 100 μl-drop of CR1aa (2) CR1aa+TCM: at 72 h after culture in CR1aa, embryos were cultured in TCM (3) TCM: zygotes were cultured in TCM. The blastocyst production rate was significantly higher (p<0.05, P<0.01) with CR1aa+TCM (42.4%) than those of CR1aa+CR1aa and TCM+TCM (31.3, 22.5%, respectively). To examine the effect of number of oocytes on the IVMFC, zygotes were cultured 2-9 or 10 embryos per 100 μl drop in CR1aa+TCM for 11 days. The proportion of embryos cleaved and blastocysts were not different between embryos derived from 10 embryos/drop and 2-9 embryos/drop (80.9% vs. 13.6% and 65.5% vs. 13.7%, respectively). Four blastocysts developed from 2-9 embryos per drop were transferred into recipients. One of the 4 recipients became pregnant. One male live calf was born at 286 day. These results indicate that CR1aa+TCM are effective media for IVC of a small number of embryos per drop, and embryos from a small number of embryos per drop have the developmental capacity to become calf.","PeriodicalId":90599,"journal":{"name":"Journal of mammalian ova research","volume":"26 1","pages":"52-55"},"PeriodicalIF":0.0,"publicationDate":"2004-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75902189","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

The Art of ICSI: Animal Eggs ICSI的艺术:动物卵子

Journal of mammalian ova research Pub Date : 2004-01-01 DOI: 10.1274/JMOR.21.56

M. Takenaka, T. Horiuchi

引用次数: 0

Prenatal Diagnosis of Chromosomal Abnormalities through Amniocentesis 羊膜穿刺术产前诊断染色体异常

Journal of mammalian ova research Pub Date : 2004-01-01 DOI: 10.1274/JMOR.21.18

H. Sago

引用次数: 21

How to Use and Maintain the Microscopes Used in ICSI ICSI中使用的显微镜如何使用和维护

Journal of mammalian ova research Pub Date : 2004-01-01 DOI: 10.1274/JMOR.21.225

Takaaki Tanaka

引用次数: 0

Sequential Culture Method of Human Embryos 人类胚胎序贯培养方法

Journal of mammalian ova research Pub Date : 2004-01-01 DOI: 10.1274/JMOR.21.220

S. Ieda, M. Kuwayama

引用次数: 0

Intracytoplasmic Sperm Injection in the Pig: Sperm-based Approaches to Improve Viability of Embryos Generated by ICSI 猪卵胞浆内单精子注射:基于精子的方法提高ICSI产生的胚胎存活率

Journal of mammalian ova research Pub Date : 2004-01-01 DOI: 10.1274/JMOR.21.185

M. Nakai, K. Kikuchi, Maiko Koichi, N. Kashiwazaki

引用次数: 1

Activation and Development of Pig Oocytes after Microinjection of Crude Sperm Extract 粗精子提取物微注射后猪卵母细胞的活化和发育

Journal of mammalian ova research Pub Date : 2004-01-01 DOI: 10.1274/JMOR.21.134

K. Okada, T. Miyano, M. Miyake

引用次数: 7

The Indication of ICSI ICSI的指征

Journal of mammalian ova research Pub Date : 2004-01-01 DOI: 10.1274/JMOR.21.204

Y. Hamada, Mutsuko Fujiwara, K. Takebayashi, Kentaro Takahashi, Y. Noda

{"title":"The Indication of ICSI","authors":"Y. Hamada, Mutsuko Fujiwara, K. Takebayashi, Kentaro Takahashi, Y. Noda","doi":"10.1274/JMOR.21.204","DOIUrl":"https://doi.org/10.1274/JMOR.21.204","url":null,"abstract":"The indication of ICSI is generally based on sperm analysis, past therapeutic history, and prediction of fertilization failure. In this study, we attempted to evaluate the usefulness of SMI (Sperm Motility Index) and \"split cycle\" procedure on ICSI indication. According to SMI score, patients were separated into three groups: I) Poor (0≤SMI<80, n=10), II) Medium (80≤SMI<160, n=9), III) Good (160≤SMI, n=43). Among these, no significant difference was observed in fertilization rates (Poor 56.4%, Medium 69.6%, and Good 71.1%) or in pregnancy rates (20.0%, 33.3%, and 48.8%, respectively). On the other hand, patients were separated into three groups: I) conventional IVF (n=188), II) split cycle (n=14), III) ICSI (n=72). Among these, no significant difference was observed in fertilization rates (conventional IVF 64.8%, split cycle 63.0%, and ICSI 56.0%) or in pregnancy rates (46.6%, 57.1%, and 31.7%, respectively). Surprisingly, among those in split cycle who had had fertilization failure on previous conventional IVF trial, no significant difference was found in fertilization rates between conventional IVF eggs and ICSI eggs. In such cases, split cycle might be a better choice.","PeriodicalId":90599,"journal":{"name":"Journal of mammalian ova research","volume":"87 1","pages":"204-208"},"PeriodicalIF":0.0,"publicationDate":"2004-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73464158","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Histone Deacetylase Inhibitor Trichostatin A Induces Retrogressive Chromatin Decondensation in the Germinal Vesicle of Porcine Cumulus-enclosed Oocytes 组蛋白去乙酰化酶抑制剂曲古霉素A诱导猪卵母细胞生发囊中染色质退行性去浓缩

Journal of mammalian ova research Pub Date : 2004-01-01 DOI: 10.1274/JMOR.21.110

Y. Hirao, N. Takenouchi, M. Shimizu, K. Iga, T. Miyano, T. Nagai

{"title":"The Histone Deacetylase Inhibitor Trichostatin A Induces Retrogressive Chromatin Decondensation in the Germinal Vesicle of Porcine Cumulus-enclosed Oocytes","authors":"Y. Hirao, N. Takenouchi, M. Shimizu, K. Iga, T. Miyano, T. Nagai","doi":"10.1274/JMOR.21.110","DOIUrl":"https://doi.org/10.1274/JMOR.21.110","url":null,"abstract":"In porcine growing oocytes, chromatin remains diffuse. As the oocytes approach their final size, 120 μm, the chromatin becomes partly condensed and forms a perinucleolar sheath. These changes occur simultaneously with a decrease in transcriptional activity. In many other cell types, it has been shown that the state of acetylation of nucleosome core histones is essential in chromatin remodeling and transcription so that partial chromatin condensation in oocytes may involve the recruitment of histone deacetylases. In order to test this hypothesis, porcine oocyte-cumulus cell complexes were treated with a specific inhibitor of histone deacetylases, trichostatin A (TSA). The perinucleolar sheath loosened or disappeared after 24 hr culture with 100 nM TSA, but after further culture in TSA-free medium, about 40% developed the perinucleolar sheath again. In the presence of 4 mM hypoxanthine, the decondensation induced by TSA progressed rather slowly, but continuously, for 72 hr. When oocytes were denuded before culture, spontaneous maturation occurred in the presence of TSA. Thus, the inhibitor-induced decondensation is not attributed to the inhibition of the maturation-promoting factor. These results suggest that deacetylation of histones may be involved in chromatin remodeling in oocytes near the end of the growth phase.","PeriodicalId":90599,"journal":{"name":"Journal of mammalian ova research","volume":"65 1","pages":"110-117"},"PeriodicalIF":0.0,"publicationDate":"2004-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86005572","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Xenografting of Bovine Secondary Follicles into Male and Female SCID Mice 牛次级卵泡在雌雄SCID小鼠体内的异种移植

Journal of mammalian ova research Pub Date : 2004-01-01 DOI: 10.1274/JMOR.21.157

S. Senbon, Atsushi Ota, Masao Tachibana, T. Miyano

{"title":"Xenografting of Bovine Secondary Follicles into Male and Female SCID Mice","authors":"S. Senbon, Atsushi Ota, Masao Tachibana, T. Miyano","doi":"10.1274/JMOR.21.157","DOIUrl":"https://doi.org/10.1274/JMOR.21.157","url":null,"abstract":": Xenografting of ovarian tissue into immunodeficient mice is useful in studying the dynamics of follicular development. We have demonstrated that xenografted bovine secondary follicles develop to the antral stage in female severe combined immunodeficient (SCID) mice. We did this by examining the development of bovine secondary follicles (140(cid:150)190 µ m in diameter) that had been grafted into male and female SCID mice for 4 and 6 weeks. We then compared the results for the two groups. The rate of surviving follicles in the grafts was similar in male and female mice, but the survival rate of oocytes was lower in male mice in these follicles, especially the antral follicles. In addition, the basement membranes of relatively large follicles were thinner and torn in the male mice, and erythrocytes had invaded the follicular cavity. The mean diameters of surviving follicles and oocytes were significantly larger in both male and female mice than before grafting. In female mice, the diameter of antral follicles increased gradually as the grafting was prolonged, although the difference was not in significant. Surviving oocytes in the follicles increased in diameter. In contrast, development of antral follicles in male mice seemed to be accelerated, but, in contrast to female mice, the mean diameters of antral follicles and surviving oocytes showed no further increase after 4 weeks of grafting. These results suggest that bovine follicles can develop in male SCID mice, but oocyte degeneration together with the follicular degeneration occurs in large antral follicles at a higher rate in males than in the females.","PeriodicalId":90599,"journal":{"name":"Journal of mammalian ova research","volume":"17 1","pages":"157-161"},"PeriodicalIF":0.0,"publicationDate":"2004-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75579027","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4