Sequential Culture Method of Human Embryos

Abstract

The blastocyst transfer (BT), transferring blastocysts following IVF, has been recently focused as an effective assisted reproductive technology in humans because it has powerful efficacy on not only prevention of multiple pregnancies but also high pregnancy rates. The BT has been already applied to about 70% in IVF cycles per year in our facility resulted in excellent clinical results. Quality of the resulting blastocyst is the most important key for the successful BT. High quality blastocysts can be produced in vitro by the sequential culture method using commercially supplied media under low oxygen atmosphere phase in a multi-gas incubator. Optimized environments of the embryo culture room (27 ° C, high humidity and bio-clean room without ultraviolet light) and completed conditions of the culture media are essential for production of the excellent blastocysts. Other fine advices for success are also introduced in the present article.