BMC Cell Biology最新文献

{"title":"Mesenchymal stem cells and myoblast differentiation under HGF and IGF-1 stimulation for 3D skeletal muscle tissue engineering.","authors":"R Witt,&nbsp;A Weigand,&nbsp;A M Boos,&nbsp;A Cai,&nbsp;D Dippold,&nbsp;A R Boccaccini,&nbsp;D W Schubert,&nbsp;M Hardt,&nbsp;C Lange,&nbsp;A Arkudas,&nbsp;R E Horch,&nbsp;J P Beier","doi":"10.1186/s12860-017-0131-2","DOIUrl":"https://doi.org/10.1186/s12860-017-0131-2","url":null,"abstract":"<p><strong>Background: </strong>Volumetric muscle loss caused by trauma or after tumour surgery exceeds the natural regeneration capacity of skeletal muscle. Hence, the future goal of tissue engineering (TE) is the replacement and repair of lost muscle tissue by newly generating skeletal muscle combining different cell sources, such as myoblasts and mesenchymal stem cells (MSCs), within a three-dimensional matrix. Latest research showed that seeding skeletal muscle cells on aligned constructs enhance the formation of myotubes as well as cell alignment and may provide a further step towards the clinical application of engineered skeletal muscle. In this study the myogenic differentiation potential of MSCs upon co-cultivation with myoblasts and under stimulation with hepatocyte growth factor (HGF) and insulin-like growth factor-1 (IGF-1) was evaluated. We further analysed the behaviour of MSC-myoblast co-cultures in different 3D matrices.</p><p><strong>Results: </strong>Primary rat myoblasts and rat MSCs were mono- and co-cultivated for 2, 7 or 14 days. The effect of different concentrations of HGF and IGF-1 alone, as well as in combination, on myogenic differentiation was analysed using microscopy, multicolour flow cytometry and real-time PCR. Furthermore, the influence of different three-dimensional culture models, such as fibrin, fibrin-collagen-I gels and parallel aligned electrospun poly-ε-caprolacton collagen-I nanofibers, on myogenic differentiation was analysed. MSCs could be successfully differentiated into the myogenic lineage both in mono- and in co-cultures independent of HGF and IGF-1 stimulation by expressing desmin, myocyte enhancer factor 2, myosin heavy chain 2 and alpha-sarcomeric actinin. An increased expression of different myogenic key markers could be observed under HGF and IGF-1 stimulation. Even though, stimulation with HGF/IGF-1 does not seem essential for sufficient myogenic differentiation. Three-dimensional cultivation in fibrin-collagen-I gels induced higher levels of myogenic differentiation compared with two-dimensional experiments. Cultivation on poly-ε-caprolacton-collagen-I nanofibers induced parallel alignment of cells and positive expression of desmin.</p><p><strong>Conclusions: </strong>In this study, we were able to myogenically differentiate MSC upon mono- and co-cultivation with myoblasts. The addition of HGF/IGF-1 might not be essential for achieving successful myogenic differentiation. Furthermore, with the development of a biocompatible nanofiber scaffold we established the basis for further experiments aiming at the generation of functional muscle tissue.</p>","PeriodicalId":9051,"journal":{"name":"BMC Cell Biology","volume":"18 1","pages":"15"},"PeriodicalIF":0.0,"publicationDate":"2017-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1186/s12860-017-0131-2","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34771360","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hinokitiol induces DNA demethylation via DNMT1 and UHRF1 inhibition in colon cancer cells.","authors":"Jung Seon Seo,&nbsp;Young Ha Choi,&nbsp;Ji Wook Moon,&nbsp;Hyeon Soo Kim,&nbsp;Sun-Hwa Park","doi":"10.1186/s12860-017-0130-3","DOIUrl":"https://doi.org/10.1186/s12860-017-0130-3","url":null,"abstract":"<p><strong>Background: </strong>DNA hypermethylation is a key epigenetic mechanism for the silencing of many genes in cancer. Hinokitiol, a tropolone-related natural compound, is known to induce apoptosis and cell cycle arrest and has anti-inflammatory and anti-tumor activities. However, the relationship between hinokitiol and DNA methylation is not clear. The aim of our study was to explore whether hinokitiol has an inhibitory ability on the DNA methylation in colon cancer cells.</p><p><strong>Results: </strong>MTT data showed that hinokitiol had higher sensitivity in colon cancer cells, HCT-116 and SW480, than in normal colon cells, CCD18Co. Hinokitiol reduced DNA methyltransferase 1 (DNMT1) and ubiquitin-like plant homeodomain and RING finger domain 1 (UHRF1) expression in HCT-116 cells. In addition, the expression of ten-eleven translocation protein 1 (TET1), a known DNA demethylation initiator, was increased by hinokitiol treatment. ELISA and FACS data showed that hinokitiol increased the 5-hydroxymethylcytosine (5hmC) level in the both colon cancer cells, but 5-methylcytosine (5mC) level was not changed. Furthermore, hinokitiol significantly restored mRNA expression of O⁶-methylguanine DNA methyltransferase (MGMT), carbohydrate sulfotransferase 10 (CHST10), and B-cell translocation gene 4 (BTG4) concomitant with reduction of methylation status in HCT-116 cells.</p><p><strong>Conclusions: </strong>These results indicate that hinokitiol may exert DNA demethylation by inhibiting the expression of DNMT1 and UHRF1 in colon cancer cells.</p>","PeriodicalId":9051,"journal":{"name":"BMC Cell Biology","volume":"18 1","pages":"14"},"PeriodicalIF":0.0,"publicationDate":"2017-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1186/s12860-017-0130-3","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34767801","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Variation in human dental pulp stem cell ageing profiles reflect contrasting proliferative and regenerative capabilities","authors":"A. Alraies, Nadia Y. A. Alaidaroos, R. Waddington, R. Moseley, A. Sloan","doi":"10.1186/s12860-017-0128-x","DOIUrl":"https://doi.org/10.1186/s12860-017-0128-x","url":null,"abstract":"","PeriodicalId":9051,"journal":{"name":"BMC Cell Biology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2017-02-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1186/s12860-017-0128-x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44529889","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Release of endothelial cell associated VEGFR2 during TGF-β modulated angiogenesis in vitro","authors":"Mai Jarad, E. Kuczynski, J. Morrison, A. Viloria‐Petit, B. Coomber","doi":"10.1186/s12860-017-0127-y","DOIUrl":"https://doi.org/10.1186/s12860-017-0127-y","url":null,"abstract":"","PeriodicalId":9051,"journal":{"name":"BMC Cell Biology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2017-01-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1186/s12860-017-0127-y","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42817137","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"miR-27b inhibits fibroblast activation via targeting TGFβ signaling pathway","authors":"Xiangming Zeng, Chaoqun Huang, Lakmini Senavirathna, Pengcheng Wang, Lin Liu","doi":"10.1186/s12860-016-0123-7","DOIUrl":"https://doi.org/10.1186/s12860-016-0123-7","url":null,"abstract":"","PeriodicalId":9051,"journal":{"name":"BMC Cell Biology","volume":"18 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2017-01-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1186/s12860-016-0123-7","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"65674052","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Intermittent hypoxia causes NOX2-dependent remodeling of atrial connexins.","authors":"Joanna Gemel, Zihan Su, Alex Gileles-Hillel, Abdelnaby Khalyfa, David Gozal, Eric C Beyer","doi":"10.1186/s12860-016-0117-5","DOIUrl":"10.1186/s12860-016-0117-5","url":null,"abstract":"<p><strong>Background: </strong>Obstructive sleep apnea has been linked to the development of heart disease and arrhythmias, including atrial fibrillation. Since altered conduction through gap junction channels can contribute to the pathogenesis of such arrhythmias, we examined the abundance and distributions of the major cardiac gap junction proteins, connexin40 (Cx40) and connexin43 (Cx43) in mice treated with sleep fragmentation or intermittent hypoxia (IH) as animal models of the components of obstructive sleep apnea.</p><p><strong>Results: </strong>Wild type C57BL/6 mice or mice lacking NADPH 2 (NOX2) oxidase activity (gp91phox(-/Y)) were exposed to room air or to SF or IH for 6 weeks. Then, the mice were sacrificed, and atria and ventricles were immediately dissected. The abundances of Cx40 or Cx43 in atria and ventricles were unaffected by SF. In contrast, immunoblots showed that the abundance of atrial Cx40 and Cx43 and ventricular Cx43 were reduced in mice exposed to IH. qRT-PCR demonstrated significant reductions of atrial Cx40 and Cx43 mRNAs. Immunofluorescence microscopy revealed that the abundance and size of gap junctions containing Cx40 or Cx43 were reduced in atria by IH treatment of mice. However, no changes of connexin abundance or gap junction size/abundance were observed in IH-treated NOX2-null mice.</p><p><strong>Conclusions: </strong>These results demonstrate that intermittent hypoxia (but not sleep fragmentation) causes reductions and remodeling of atrial Cx40 and Cx43. These alterations may contribute to the substrate for atrial fibrillation that develops in response to obstructive sleep apnea. Moreover, these connexin changes are likely generated in response to reactive oxygen species generated by NOX2.</p>","PeriodicalId":9051,"journal":{"name":"BMC Cell Biology","volume":"18 1","pages":"7"},"PeriodicalIF":0.0,"publicationDate":"2017-01-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1186/s12860-016-0117-5","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"65673670","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nuclear envelope structural proteins facilitate nuclear shape changes accompanying embryonic differentiation and fidelity of gene expression","authors":"Elizabeth R. Smith, Yue Meng, Robert Moore, Jeffrey D. Tse, Arn G. Xu, Xiang-Xi Xu","doi":"10.1186/s12860-017-0125-0","DOIUrl":"https://doi.org/10.1186/s12860-017-0125-0","url":null,"abstract":"","PeriodicalId":9051,"journal":{"name":"BMC Cell Biology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2017-01-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1186/s12860-017-0125-0","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43763698","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Erratum to: Nuclear envelope structural defect underlies the main cause of aneuploidy in ovarian carcinogenesis","authors":"C. D. Capo-chichi, T. Yeasky, Elizabeth R. Smith, Xiang-Xi Xu","doi":"10.1186/s12860-016-0124-6","DOIUrl":"https://doi.org/10.1186/s12860-016-0124-6","url":null,"abstract":"","PeriodicalId":9051,"journal":{"name":"BMC Cell Biology","volume":"11 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2017-01-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1186/s12860-016-0124-6","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"65674194","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cardiac Cx43, Cx40 and Cx45 co-assembling: involvement of connexins epitopes in formation of hemichannels and Gap junction channels","authors":"T. Desplantez","doi":"10.1186/s12860-016-0118-4","DOIUrl":"https://doi.org/10.1186/s12860-016-0118-4","url":null,"abstract":"","PeriodicalId":9051,"journal":{"name":"BMC Cell Biology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1186/s12860-016-0118-4","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44499339","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Gap junctions and hemichannels: communicating cell death in neurodevelopment and disease","authors":"A. Belousov, J. Fontes, Moises Freitas-Andrade, C. Naus","doi":"10.1186/s12860-016-0120-x","DOIUrl":"https://doi.org/10.1186/s12860-016-0120-x","url":null,"abstract":"","PeriodicalId":9051,"journal":{"name":"BMC Cell Biology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1186/s12860-016-0120-x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45964285","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}