BMC Cell Biology最新文献

{"title":"Hierarchical imaging: a new concept for targeted imaging of large volumes from cells to tissues","authors":"I. Wacker, Waldemar Spomer, Andreas Hofmann, Marlene Thaler, S. Hillmer, U. Gengenbach, Rasmus R. Schröder","doi":"10.1186/s12860-016-0122-8","DOIUrl":"https://doi.org/10.1186/s12860-016-0122-8","url":null,"abstract":"","PeriodicalId":9051,"journal":{"name":"BMC Cell Biology","volume":"17 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2016-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1186/s12860-016-0122-8","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"65673804","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nuclear envelope structural defect underlies the main cause of aneuploidy in ovarian carcinogenesis.","authors":"Callinice D Capo-Chichi,&nbsp;Toni M Yeasky,&nbsp;Elizabeth R Smith,&nbsp;Xiang-Xi Xu","doi":"10.1186/s12860-016-0114-8","DOIUrl":"https://doi.org/10.1186/s12860-016-0114-8","url":null,"abstract":"<p><strong>Background: </strong>The Cancer Atlas project has shown that p53 is the only commonly (96 %) mutated gene found in high-grade serous epithelial ovarian cancer, the major histological subtype. Another general genetic change is extensive aneuploidy caused by chromosomal numerical instability, which is thought to promote malignant transformation. Conventionally, aneuploidy is thought to be the result of mitotic errors and chromosomal nondisjunction during mitosis. Previously, we found that ovarian cancer cells often lost or reduced nuclear lamina proteins lamin A/C, and suppression of lamin A/C in cultured ovarian epithelial cells leads to aneuploidy. Following up, we investigated the mechanisms of lamin A/C-suppression in promoting aneuploidy and synergy with p53 inactivation.</p><p><strong>Results: </strong>We found that suppression of lamin A/C by siRNA in human ovarian surface epithelial cells led to frequent nuclear protrusions and formation of micronuclei. Lamin A/C-suppressed cells also often underwent mitotic failure and furrow regression to form tetraploid cells, which frequently underwent aberrant multiple polar mitosis to form aneuploid cells. In ovarian surface epithelial cells isolated from p53 null mice, transient suppression of lamin A/C produced massive aneuploidy with complex karyotypes, and the cells formed malignant tumors when implanted in mice.</p><p><strong>Conclusions: </strong>Based on the results, we conclude that a nuclear envelope structural defect, such as the loss or reduction of lamin A/C proteins, leads to aneuploidy by both the formation of tetraploid intermediates following mitotic failure, and the reduction of chromosome (s) following nuclear budding and subsequent loss of micronuclei. We suggest that the nuclear envelope defect, rather than chromosomal unequal distribution during cytokinesis, is the main cause of aneuploidy in ovarian cancer development.</p>","PeriodicalId":9051,"journal":{"name":"BMC Cell Biology","volume":" ","pages":"37"},"PeriodicalIF":0.0,"publicationDate":"2016-11-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1186/s12860-016-0114-8","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39980691","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A functional endosomal pathway is necessary for lysosome biogenesis in Drosophila","authors":"Anne-Claire Jacomin, M. Fauvarque, E. Taillebourg","doi":"10.1186/s12860-016-0115-7","DOIUrl":"https://doi.org/10.1186/s12860-016-0115-7","url":null,"abstract":"","PeriodicalId":9051,"journal":{"name":"BMC Cell Biology","volume":"17 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2016-11-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1186/s12860-016-0115-7","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"65673566","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Expressed alleles of imprinted IGF2, DLK1 and MEG3 colocalize in 3D-preserved nuclei of porcine fetal cells","authors":"Y. Lahbib-Mansais, H. Barasc, M. Marti-Marimon, F. Mompart, E. Iannuccelli, D. Robelin, J. Riquet, M. Yerle-Bouissou","doi":"10.1186/s12860-016-0113-9","DOIUrl":"https://doi.org/10.1186/s12860-016-0113-9","url":null,"abstract":"","PeriodicalId":9051,"journal":{"name":"BMC Cell Biology","volume":"17 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2016-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1186/s12860-016-0113-9","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"65673928","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nuclear and structural dynamics during the establishment of a specialized effector-secreting cell by Magnaporthe oryzae in living rice cells","authors":"E. Shipman, Kiersun Jones, C. B. Jenkinson, D. W. Kim, Jie Zhu, C. Khang","doi":"10.1186/s12860-017-0126-z","DOIUrl":"https://doi.org/10.1186/s12860-017-0126-z","url":null,"abstract":"","PeriodicalId":9051,"journal":{"name":"BMC Cell Biology","volume":"18 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2016-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1186/s12860-017-0126-z","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"65674432","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Loss of hif-1 promotes resistance to the exogenous mitochondrial stressor ethidium bromide in Caenorhabditis elegans","authors":"Muntasir Kamal, D. D’Amora, T. Kubiseski","doi":"10.1186/s12860-016-0112-x","DOIUrl":"https://doi.org/10.1186/s12860-016-0112-x","url":null,"abstract":"","PeriodicalId":9051,"journal":{"name":"BMC Cell Biology","volume":"17 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2016-09-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1186/s12860-016-0112-x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"65673516","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"High resolution imaging reveals heterogeneity in chromatin states between cells that is not inherited through cell division.","authors":"David Dickerson,&nbsp;Marek Gierliński,&nbsp;Vijender Singh,&nbsp;Etsushi Kitamura,&nbsp;Graeme Ball,&nbsp;Tomoyuki U Tanaka,&nbsp;Tom Owen-Hughes","doi":"10.1186/s12860-016-0111-y","DOIUrl":"https://doi.org/10.1186/s12860-016-0111-y","url":null,"abstract":"<p><strong>Background: </strong>Genomes of eukaryotes exist as chromatin, and it is known that different chromatin states can influence gene regulation. Chromatin is not a static structure, but is known to be dynamic and vary between cells. In order to monitor the organisation of chromatin in live cells we have engineered fluorescent fusion proteins which recognize specific operator sequences to tag pairs of syntenic gene loci. The separation of these loci was then tracked in three dimensions over time using fluorescence microscopy.</p><p><strong>Results: </strong>We established a work flow for measuring the distance between two fluorescently tagged, syntenic gene loci with a mean measurement error of 63 nm. In general, physical separation was observed to increase with increasing genomic separations. However, the extent to which chromatin is compressed varies for different genomic regions. No correlation was observed between compaction and the distribution of chromatin markers from genomic datasets or with contacts identified using capture based approaches. Variation in spatial separation was also observed within cells over time and between cells. Differences in the conformation of individual loci can persist for minutes in individual cells. Separation of reporter loci was found to be similar in related and unrelated daughter cell pairs.</p><p><strong>Conclusions: </strong>The directly observed physical separation of reporter loci in live cells is highly dynamic both over time and from cell to cell. However, consistent differences in separation are observed over some chromosomal regions that do not correlate with factors known to influence chromatin states. We conclude that as yet unidentified parameters influence chromatin configuration. We also find that while heterogeneity in chromatin states can be maintained for minutes between cells, it is not inherited through cell division. This may contribute to cell-to-cell transcriptional heterogeneity.</p>","PeriodicalId":9051,"journal":{"name":"BMC Cell Biology","volume":"17 1","pages":"33"},"PeriodicalIF":0.0,"publicationDate":"2016-09-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1186/s12860-016-0111-y","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34374554","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A specific FMNL2 isoform is up-regulated in invasive cells.","authors":"Christine Péladeau,&nbsp;Allan Heibein,&nbsp;Melissa T Maltez,&nbsp;Sarah J Copeland,&nbsp;John W Copeland","doi":"10.1186/s12860-016-0110-z","DOIUrl":"https://doi.org/10.1186/s12860-016-0110-z","url":null,"abstract":"<p><strong>Background: </strong>Formins are a highly conserved family of cytoskeletal remodeling proteins. A growing body of evidence suggests that formins play key roles in the progression and spread of a variety of cancers. There are 15 human formin proteins and of these the Diaphanous-Related Formins (DRFs) are the best characterized. Included in the DRFs are the Formin-Like proteins, FMNL1, 2 & 3, each of which have been strongly implicated in driving tumorigenesis and metastasis of specific tumors. In particular, increased FMNL2 expression correlates with increased invasiveness of colorectal cancer (CRC) in vivo and for a variety of CRC cell-lines in vitro. FMNL2 expression is also required for invasive cell motility in other cancer cell-lines. There are multiple alternatively spliced isoforms of FMNL2 and it is predicted that the encoded proteins will differ in their regulation, subcellular localization and in their ability to regulate cytoskeletal dynamics.</p><p><strong>Results: </strong>Using RT-PCR we identified four FMNL2 isoforms expressed in CRC and melanoma cell-lines. We find that a previously uncharacterized FMNL2 isoform is predominantly expressed in a variety of melanoma and CRC cell lines; this isoform is also more effective in driving 3D motility. Building on previous reports, we also show that FMNL2 is required for invasion in A375 and WM266.4 melanoma cells.</p><p><strong>Conclusions: </strong>Taken together, these results suggest that FMNL2 is likely to be generally required in melanoma cells for invasion, that a specific isoform of FMNL2 is up-regulated in invasive CRC and melanoma cells and this isoform is the most effective at facilitating invasion.</p>","PeriodicalId":9051,"journal":{"name":"BMC Cell Biology","volume":"17 1","pages":"32"},"PeriodicalIF":0.0,"publicationDate":"2016-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1186/s12860-016-0110-z","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34350143","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prolactin-induced PAK1 tyrosyl phosphorylation promotes FAK dephosphorylation, breast cancer cell motility, invasion and metastasis.","authors":"Alan Hammer,&nbsp;Maria Diakonova","doi":"10.1186/s12860-016-0109-5","DOIUrl":"https://doi.org/10.1186/s12860-016-0109-5","url":null,"abstract":"<p><strong>Background: </strong>The serine/threonine kinase PAK1 is an important regulator of cell motility. Both PAK1 and the hormone/cytokine prolactin (PRL) have been implicated in breast cancer cell motility, however, the exact mechanisms guiding PRL/PAK1 signaling in breast cancer cells have not been fully elucidated. Our lab has previously demonstrated that PRL-activated tyrosine kinase JAK2 phosphorylates PAK1 on tyrosines 153, 201, and 285, and that tyrosyl phosphorylated PAK1 (pTyr-PAK1) augments migration and invasion of breast cancer cells.</p><p><strong>Results: </strong>Here we further investigate the mechanisms by which pTyr-PAK1 enhances breast cancer cell motility in response to PRL. We demonstrate a distinct reduction in PRL-induced FAK auto-phosphorylation in T47D and TMX2-28 breast cancer cells overexpressing wild-type PAK1 (PAK1 WT) when compared to cells overexpressing either GFP or phospho-tyrosine-deficient mutant PAK1 (PAK1 Y3F). Furthermore, pTyr-PAK1 phosphorylates MEK1 on Ser298 resulting in subsequent ERK1/2 activation. PRL-induced FAK auto-phosphorylation is rescued in PAK1 WT cells by inhibiting tyrosine phosphatases and tyrosine phosphatase inhibition abrogates cell motility and invasion in response to PRL. siRNA-mediated knockdown of the tyrosine phosphatase PTP-PEST rescues FAK auto-phosphorylation in PAK1 WT cells and reduces both cell motility and invasion. Finally, we provide evidence that PRL-induced pTyr-PAK1 stimulates tumor cell metastasis in vivo.</p><p><strong>Conclusion: </strong>These data provide insight into the mechanisms guiding PRL-mediated breast cancer cell motility and invasion and highlight a significant role for pTyr-PAK1 in breast cancer metastasis.</p>","PeriodicalId":9051,"journal":{"name":"BMC Cell Biology","volume":"17 1","pages":"31"},"PeriodicalIF":0.0,"publicationDate":"2016-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1186/s12860-016-0109-5","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34380751","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Aneuploidy screening of embryonic stem cell clones by metaphase karyotyping and droplet digital polymerase chain reaction.","authors":"Gemma F Codner, Loic Lindner, Adam Caulder, Marie Wattenhofer-Donzé, Adam Radage, Annelyse Mertz, Benjamin Eisenmann, Joffrey Mianné, Edward P Evans, Colin V Beechey, Martin D Fray, Marie-Christine Birling, Yann Hérault, Guillaume Pavlovic, Lydia Teboul","doi":"10.1186/s12860-016-0108-6","DOIUrl":"10.1186/s12860-016-0108-6","url":null,"abstract":"<p><strong>Background: </strong>Karyotypic integrity is essential for the successful germline transmission of alleles mutated in embryonic stem (ES) cells. Classical methods for the identification of aneuploidy involve cytological analyses that are both time consuming and require rare expertise to identify mouse chromosomes.</p><p><strong>Results: </strong>As part of the International Mouse Phenotyping Consortium, we gathered data from over 1,500 ES cell clones and found that the germline transmission (GLT) efficiency of clones is compromised when over 50 % of cells harbour chromosome number abnormalities. In JM8 cells, chromosomes 1, 8, 11 or Y displayed copy number variation most frequently, whilst the remainder generally remain unchanged. We developed protocols employing droplet digital polymerase chain reaction (ddPCR) to accurately quantify the copy number of these four chromosomes, allowing efficient triage of ES clones prior to microinjection. We verified that assessments of aneuploidy, and thus decisions regarding the suitability of clones for microinjection, were concordant between classical cytological and ddPCR-based methods. Finally, we improved the method to include assay multiplexing so that two unstable chromosomes are counted simultaneously (and independently) in one reaction, to enhance throughput and further reduce the cost.</p><p><strong>Conclusion: </strong>We validated a PCR-based method as an alternative to classical karyotype analysis. This technique enables laboratories that are non-specialist, or work with large numbers of clones, to precisely screen ES cells for the most common aneuploidies prior to microinjection to ensure the highest level of germline transmission potential. The application of this method allows early exclusion of aneuploid ES cell clones in the ES cell to mouse conversion process, thus improving the chances of obtaining germline transmission and reducing the number of animals used in failed microinjection attempts. This method can be applied to any other experiments that require accurate analysis of the genome for copy number variation (CNV).</p>","PeriodicalId":9051,"journal":{"name":"BMC Cell Biology","volume":"17 1","pages":"30"},"PeriodicalIF":0.0,"publicationDate":"2016-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4974727/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34345808","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}